LOCUS       NM_172272               2228 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus prolyl-tRNA synthetase (mitochondrial)(putative)
            (Pars2), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_172272
VERSION     NM_172272.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929585.11 and BY768753.1.
            
            On Apr 4, 2007 this sequence version replaced NM_172272.1.
            
            Transcript Variant: This variant (1) differs in the 5' UTR and the
            5' coding region and initiates translation at an alternate start
            codon, compared to variant 3. Variants 1 and 4 encode the same
            isoform (1), which is shorter at the N-terminus compared to isoform
            2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038817.1, SRR7345562.937397.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AL929585.11        90561-90601
            42-322              BY768753.1         2-282
            323-2228            AL929585.11        92869-94774
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 49.67 cM"
     gene            1..2228
                     /gene="Pars2"
                     /note="prolyl-tRNA synthetase (mitochondrial)(putative)"
                     /db_xref="GeneID:230577"
                     /db_xref="MGI:MGI:2386296"
     exon            1..63
                     /gene="Pars2"
                     /inference="alignment:Splign:2.1.0"
     exon            64..2228
                     /gene="Pars2"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..1504
                     /gene="Pars2"
                     /EC_number="6.1.1.15"
                     /note="isoform 1 is encoded by transcript variant 1; class
                     II tRNA synthetase; probable prolyl-tRNA synthetase,
                     mitochondrial; proRS; proline--tRNA ligase; probable
                     proline--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="probable proline--tRNA ligase, mitochondrial
                     isoform 1"
                     /protein_id="NP_758476.2"
                     /db_xref="CCDS:CCDS18423.1"
                     /db_xref="GeneID:230577"
                     /db_xref="MGI:MGI:2386296"
                     /translation="MEGLLTRCRTLSALAACSLRHCRYIIHKCYHRAPGRGQRLVLSR
                     MFQPQNLREDQVLSLEGRASDLTCKSQRLMLQVGLILPASPGCYHLMPYTVRAVEKLV
                     RVIDQEMQAIGGQKINMPSLSPAELWRATSRWDLMGRELLRLRDRHGKEYCLGPTHEE
                     AVTALVASQKKLSYKQLPLLLYQVTRKFRDEPRPRFGLLRGREFYMKDMYTFDSSSEA
                     AQETYGLVCDAYCRLFDRLGLQWMKARADVGSIGGTMSHEFQLPVDIGEDRLVVCPSC
                     HFSANTEILDLSQKICPDCQGPLTETKGIEVGHTFYLSTKYSSIFNALFTNAHGESLL
                     AEMGCYGLGVTRILAAAIEVLSTEDCIRWPRLLAPYQVCVIPPKKGSKETAATEIVEK
                     LYDDIMEAVPQLRGEVLLDDRTHLTIGNRLKDANKLGYPFVIIAGKRALEDPAHFEVW
                     SQNTGEVVFLTKEGVMELLTGVHVV"
     regulatory      2204..2209
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pars2"
                     /note="hexamer: AATAAA"
     polyA_site      2228
                     /gene="Pars2"
                     /note="major polyA site"
ORIGIN      
        1 gaggctccgg ccatgtcggc gcaggcgcac tggctttgcg tgactgaaga gcgcccgact
       61 caggtgccag ggtgtcatgg aaggtctgct gacaagatgc agaacactgt ctgccttggc
      121 tgcctgcagc ctccgtcact gcagatacat catccacaag tgttaccatc gtgcgcccgg
      181 gagagggcag cgcttggtat tatcccgcat gttccagcct cagaaccttc gggaagacca
      241 ggtgctttct ctggagggca gagctagcga cctgacctgt aagagccagc ggctgatgct
      301 gcaagtgggc ctgatccttc cagccagccc tggctgttac cacctcatgc cctatacagt
      361 gcgtgccgtg gagaagcttg tgcgggtgat agaccaggag atgcaggcca tcggcgggca
      421 gaaaatcaac atgcccagcc tcagcccagc tgagctgtgg cgggccacca gccgctggga
      481 cctgatgggc agggagctgc tgagactgag agacaggcac ggcaaagagt actgcttggg
      541 accgactcat gaggaagcgg ttacagccct ggttgcctcc cagaagaaac tttcctacaa
      601 gcagctcccc ttgttgttgt accaggttac aaggaagttt cgggatgagc ccaggccccg
      661 gtttggtctt ctccgtggcc gggagttcta tatgaaggac atgtacacct ttgattcctc
      721 ctctgaggct gcccaggaaa cctacggcct ggtgtgtgat gcttactgta ggctgtttga
      781 caggctggga ttgcaatgga tgaaggccag ggcagatgtg ggcagcatcg ggggcacgat
      841 gtcccatgaa ttccagctac cagtggacat tggagaggac cgccttgtgg tctgtcccag
      901 ctgccacttc tccgccaaca cagagatact agacttgtca caaaaaatct gccctgactg
      961 ccagggtcca ctgacagaaa ccaaaggcat tgaggtgggg cacacattct accttagtac
     1021 caagtactct tccattttca atgccctctt taccaatgcc catggggaat ctttgctggc
     1081 tgaaatgggg tgctacgggc tgggagtcac tcggatcctg gctgcagcca tcgaagttct
     1141 ctctacggaa gactgcatcc gctggccccg cctccttgcc ccttaccaag tctgtgtcat
     1201 cccccctaag aaaggcagca aggagacagc agccacagag attgtggaga agttatatga
     1261 tgacatcatg gaggctgtgc cccagctccg aggggaggtc ctgctggacg acaggaccca
     1321 cctgaccatt ggaaacagac tgaaagatgc caacaagctt ggctacccct ttgtgatcat
     1381 tgcgggcaag agggccttgg aggacccagc acactttgag gtttggtctc agaacactgg
     1441 tgaggtggtc ttcctcacca aagagggagt catggaacta ctaactggag tgcatgttgt
     1501 ctagggggcc aaccacacct gcccctagga ttggtgtcca ctgtaacact gtagttccct
     1561 ccacttctgt cctgggtggt ttctccagaa gtggcttctc gtggaagggg agagcgtgtt
     1621 aggaaaaact ctactcctcc cttgtgatgg tttgtatgta gccattaact ccgtcctccc
     1681 tgggctagtt gtgctaccat acaccgttcc ttttacatgt ggacacttct gttaggaggg
     1741 ctgaaaggtg gcaaagagac ccaggtcact ttcccttgag ctgagtttct gggtgtaaga
     1801 tgagaatgct tgaaagctgg ggccatgctt ccccaacccc accccacagt ctgagcacac
     1861 gccttgcatt tcattcaatc cagatagtgg ggctaccggc ccttctcctc tgagaagctg
     1921 ggagccgcat agtctggaac ctggctgctg ctaattcact ggtgactcga cacacccaca
     1981 cccctgctgg ggcctgagac tcccttctgg atgtggagat ggagttgggt ctgtgaggtc
     2041 ctagggctgg ctctcactga gcctccacaa gacacatgga tctggtcgct catgcctcta
     2101 agcttcctca gaagttgtca taattgccag atggcccaaa gctacctcca gatacctgac
     2161 cttactgggt cacttcagca ctgagatcat gttctgttga ttgaataaag tcaagccttt
     2221 ggggtgaa
//
