LOCUS       NM_011863               2503 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus 3'-phosphoadenosine 5'-phosphosulfate synthase 1
            (Papss1), transcript variant 1, mRNA.
ACCESSION   NM_011863
VERSION     NM_011863.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Schroder,E., Gebel,L., Eremeev,A.A., Morgner,J., Grum,D.,
            Knauer,S.K., Bayer,P. and Mueller,J.W.
  TITLE     Human PAPS synthase isoforms are dynamically regulated enzymes with
            access to nucleus and cytoplasm
  JOURNAL   PLoS One 7 (1), e29559 (2012)
   PUBMED   22242175
REFERENCE   3  (bases 1 to 2503)
  AUTHORS   Capellini,T.D., Dunn,M.P., Passamaneck,Y.J., Selleri,L. and Di
            Gregorio,A.
  TITLE     Conservation of notochord gene expression across chordates:
            insights from the Leprecan gene family
  JOURNAL   Genesis 46 (11), 683-696 (2008)
   PUBMED   18798549
REFERENCE   4  (bases 1 to 2503)
  AUTHORS   Stelzer,C., Brimmer,A., Hermanns,P., Zabel,B. and Dietz,U.H.
  TITLE     Expression profile of Papss2 (3'-phosphoadenosine 5'-phosphosulfate
            synthase 2) during cartilage formation and skeletal development in
            the mouse embryo
  JOURNAL   Dev Dyn 236 (5), 1313-1318 (2007)
   PUBMED   17436279
REFERENCE   5  (bases 1 to 2503)
  AUTHORS   Singh,B. and Schwartz,N.B.
  TITLE     Identification and functional characterization of the novel
            BM-motif in the murine phosphoadenosine phosphosulfate (PAPS)
            synthetase
  JOURNAL   J Biol Chem 278 (1), 71-75 (2003)
   PUBMED   12414806
  REMARK    GeneRIF: Data illustrate the functional importance of several
            highly conserved residues in the active site of phosphoadenosine
            phosphosulfate synthetase.
REFERENCE   6  (bases 1 to 2503)
  AUTHORS   Beccari,T., Bibi,L., Stinchi,S., Stirling,J.L. and Orlacchio,A.
  TITLE     Mouse beta-mannosidase: cDNA cloning, expression, and chromosomal
            localization
  JOURNAL   Biosci Rep 21 (3), 315-323 (2001)
   PUBMED   11892998
REFERENCE   7  (bases 1 to 2503)
  AUTHORS   Xu,Z.H., Otterness,D.M., Freimuth,R.R., Carlini,E.J., Wood,T.C.,
            Mitchell,S., Moon,E., Kim,U.J., Xu,J.P., Siciliano,M.J. and
            Weinshilboum,R.M.
  TITLE     Human 3'-phosphoadenosine 5'-phosphosulfate synthetase 1 (PAPSS1)
            and PAPSS2: gene cloning, characterization and chromosomal
            localization
  JOURNAL   Biochem Biophys Res Commun 268 (2), 437-444 (2000)
   PUBMED   10679223
REFERENCE   8  (bases 1 to 2503)
  AUTHORS   Kurima,K., Warman,M.L., Krishnan,S., Domowicz,M., Krueger,R.C. Jr.,
            Deyrup,A. and Schwartz,N.B.
  TITLE     A member of a family of sulfate-activating enzymes causes murine
            brachymorphism
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8681-8685 (1998)
   PUBMED   9671738
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1998 Sep 29;95(20):12071]
REFERENCE   9  (bases 1 to 2503)
  AUTHORS   Deyrup,A.T., Krishnan,S., Cockburn,B.N. and Schwartz,N.B.
  TITLE     Deletion and site-directed mutagenesis of the ATP-binding motif
            (P-loop) in the bifunctional murine ATP-sulfurylase/adenosine
            5'-phosphosulfate kinase enzyme
  JOURNAL   J Biol Chem 273 (16), 9450-9456 (1998)
   PUBMED   9545271
REFERENCE   10 (bases 1 to 2503)
  AUTHORS   Li,H., Deyrup,A., Mensch,J.R. Jr., Domowicz,M., Konstantinidis,A.K.
            and Schwartz,N.B.
  TITLE     The isolation and characterization of cDNA encoding the mouse
            bifunctional ATP sulfurylase-adenosine 5'-phosphosulfate kinase
  JOURNAL   J Biol Chem 270 (49), 29453-29459 (1995)
   PUBMED   7493984
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127686.4.
            
            On Aug 10, 2022 this sequence version replaced NM_011863.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK169124.1, AK168982.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               AC127686.4         62537-62655
            120-234             AC127686.4         76983-77097
            235-470             AC127686.4         80053-80288
            471-609             AC127686.4         80759-80897
            610-728             AC127686.4         82720-82838
            729-842             AC127686.4         97537-97650
            843-954             AC127686.4         99647-99758
            955-1160            AC127686.4         103517-103722
            1161-1296           AC127686.4         105000-105135
            1297-1565           AC127686.4         116693-116961
            1566-1795           AC127686.4         129258-129487
            1796-2503           AC127686.4         140636-141343
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 61.05 cM"
     gene            1..2503
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /note="3'-phosphoadenosine 5'-phosphosulfate synthase 1"
                     /db_xref="GeneID:23971"
                     /db_xref="MGI:MGI:1330587"
     exon            1..119
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1934
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /EC_number="2.7.1.25"
                     /EC_number="2.7.7.4"
                     /note="isoform a is encoded by transcript variant 1;
                     bifunctional 3'-phosphoadenosine 5'-phosphosulfate
                     synthase 1; PAPS synthase 1; sulfurylase kinase 1"
                     /codon_start=1
                     /product="bifunctional 3'-phosphoadenosine
                     5'-phosphosulfate synthase 1 isoform a"
                     /protein_id="NP_035993.1"
                     /db_xref="CCDS:CCDS38641.1"
                     /db_xref="GeneID:23971"
                     /db_xref="MGI:MGI:1330587"
                     /translation="MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVV
                     GTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFS
                     PEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDA
                     PLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVE
                     LLQERDIVPVDASYEVKELYVPENKLHLAKTDAEALPALKINKVDMQWVQVLAEGWAT
                     PLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALVYEGRR
                     VAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVLEQGDWLIGGDLQVLDRIYWNDG
                     LDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLL
                     LHPLGGWTKDDDVPLMWRMKQHAAVLEEGILDPETTVVAIFPSPMMYAGPTEVQWHCR
                     ARMVAGANFYIVGRDPAGMPHPETGKDLYEPTHGAKVLTMAPGLITLEIVPFRVAAYN
                     KKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLVEYYKSLEKA
                     "
     exon            120..234
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            235..470
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            471..609
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            610..728
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            729..842
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            843..954
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1160
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1296
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1565
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1795
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..2503
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2481..2486
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /note="hexamer: AATAAA"
     polyA_site      2503
                     /gene="Papss1"
                     /gene_synonym="Asapk; SK1"
                     /note="major polyA site"
ORIGIN      
        1 ctctctccca ctgctcggcc ccggggctct cagcagcagt gcatccgcgc tccgcggcca
       61 tggagattcc tgggagtctg tgcaagaaag tcaagctgag caataacgcg cagaactggg
      121 ggatgcagag agcaaccaac gtcacctatc aagcccacca tgtcagcagg aataagagag
      181 ggcaggtggt ggggaccaga ggtggcttcc gtggttgcac agtttggctg acaggattgt
      241 ccggagcagg gaagacgacc gtgagcatgg ctctggagga gtacctggtg tgccacggca
      301 ttccgtgcta cactttggat ggtgacaaca tccgccaagg actcaataag aacctcggct
      361 tcagtcctga ggacagagaa gagaacgttc gccgcatagc tgaggtggcg aagctgtttg
      421 cagatgctgg cttagtgtgt atcaccagct ttatatcgcc ttacacacag gatcgcaaca
      481 acgcaaggca gattcatgag ggtgcaagct tgcccttctt tgaagttttt gtcgatgctc
      541 ctctgcatgt ctgtgagcag agggatgtca aaggcctcta caagaaggcg cgggcagggg
      601 agataaaagg cttcactggc atcgattctg agtatgagaa acccgaggcc ccggagctgg
      661 tgctgaaaac ggattcctgt gacgtcaacg actgcgtcca gcaggttgtg gagcttcttc
      721 aggaacggga catcgtccct gtggatgctt cctatgaagt gaaagagcta tatgtgccag
      781 agaataaact tcacttggcc aaaactgatg cagaagcctt accagccctg aaaatcaata
      841 aagtggatat gcagtgggtg caggttttgg cagaaggttg ggcgactcct ctgaacggct
      901 tcatgagaga gagggagtac ttgcagtgcc tccatttcga ttgtcttctg gatggaggcg
      961 tcatcaactt atcggtgcct atagttctga cagctacgca cgaggataag gagaggctgg
     1021 acggctgcac cgcgttcgct ctggtgtatg agggccgccg cgtggccatc cttcggaatc
     1081 ctgaattttt tgagcaccgg aaagaggagc ggtgtgccag acagtgggga acaacatgca
     1141 agaaccaccc ctacatcaag atggttctgg aacaagggga ttggctgatt ggaggagatc
     1201 ttcaagtcct ggaccggatt tactggaatg atggtcttga tcagtaccgc cttaccccga
     1261 cggagctcaa gcagaagttt aaagatatga acgctgatgc tgtctttgca tttcagttgc
     1321 gcaacccagt gcacaacggg cacgctctgt taatgcagga tacccacaag cagcttctgg
     1381 agaggggcta ccggcgccct gtcctgctcc tccatcctct tggtggctgg acgaaggatg
     1441 acgatgtccc tctgatgtgg cgtatgaagc agcacgctgc agtgctggag gagggcatcc
     1501 tggatcctga aacgacagtg gtggccattt tcccgtctcc tatgatgtat gctgggccaa
     1561 ccgaggtcca gtggcactgc agggcgcgga tggtggccgg agccaatttt tatattgttg
     1621 gacgagaccc tgctggcatg cctcatccgg agacagggaa ggacctctat gagccaacac
     1681 atggtgccaa agtgctgacg atggccccag gcctgattac cttggaaatc gttcccttcc
     1741 gagttgcagc ttacaacaag aaaaagaagc ggatggacta ctatgactct gagcaccacg
     1801 aagacttcga gtttatttca ggaacgagga tgcgcaagct ggcacgcgaa ggccagaaac
     1861 ctccagaggg cttcatggcc cccaaggcct ggactgtgct ggtagagtac tacaagtcct
     1921 tagagaaagc ctaactgctg agctgggcac tctgcctggg acgcatggct gagtaacaga
     1981 ggggaccacg cagccaccga cagcccttct ctgcggttgt ctgactggac acacttcctg
     2041 aaatcaggcc gtttccctcc gcgtgcattg gttttggtct gtctttcagt tctgtttcaa
     2101 actagtgtaa caccacgctg gctctaatgt atcttttcca cttattgtta atattcttat
     2161 aatacgattt taaaaatctc gcctttttat attgtattta tgcttctgtc ttacaatttt
     2221 tccaagctga tttattagtt ataaccagta atacacacat catctagctt ttcattcttt
     2281 aagtaagggg gaaaaaatca ctaaacaata aagttggcta gaccttgttt ggggaatttt
     2341 acaaatttta taaactatct gtagcaatta gggtttttat tttacattaa aaatgtaaac
     2401 tgctccccct ctcccttgca gccctacgtg ttgaggttct cttgcaatct gtgttaagta
     2461 ctgaatgaat gccttgattc aataaaatta attttttgac tta
//
