LOCUS       NM_001085376            8975 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus pappalysin 2 (Pappa2), mRNA.
ACCESSION   NM_001085376 XM_355248
VERSION     NM_001085376.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Basu,S., Mautner,L., Whiting,K., Hasenpusch-Theil,K., Borkowska,M.
            and Theil,T.
  TITLE     Identification of Pappa and Sall3 as Gli3 direct target genes
            acting downstream of cilia signaling in corticogenesis
  JOURNAL   Cereb Cortex 34 (12) (2024)
   PUBMED   39716738
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Wang,J., Noguchi,S., Takizawa,T., Negishi,Y., Morita,R., Luo,S.S.
            and Takizawa,T.
  TITLE     Placenta-specific lncRNA 1600012P17Rik is expressed in
            spongiotrophoblast and glycogen trophoblast cells of mouse placenta
  JOURNAL   Histochem Cell Biol 158 (1), 65-78 (2022)
   PUBMED   35486179
REFERENCE   3  (bases 1 to 8975)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8975)
  AUTHORS   Rubio,L., Vargas,A., Rivera,P., Lopez-Gambero,A.J., Tovar,R.,
            Christians,J.K., Martin-de-Las-Heras,S., Rodriguez de Fonseca,F.,
            Chowen,J.A., Argente,J. and Suarez,J.
  TITLE     Recombinant IGF-1 Induces Sex-Specific Changes in Bone Composition
            and Remodeling in Adult Mice with Pappa2 Deficiency
  JOURNAL   Int J Mol Sci 22 (8), 4048 (2021)
   PUBMED   33919940
  REMARK    GeneRIF: Recombinant IGF-1 Induces Sex-Specific Changes in Bone
            Composition and Remodeling in Adult Mice with Pappa2 Deficiency.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8975)
  AUTHORS   Lloyd,K.A., Parsons,B.N., Burkitt,M.D., Moore,A.R.,
            Papoutsopoulou,S., Boyce,M., Duckworth,C.A., Exarchou,K., Howes,N.,
            Rainbow,L., Fang,Y., Oxvig,C., Dodd,S., Varro,A., Hall,N. and
            Pritchard,D.M.
  TITLE     Netazepide Inhibits Expression of Pappalysin 2 in Type 1 Gastric
            Neuroendocrine Tumors
  JOURNAL   Cell Mol Gastroenterol Hepatol 10 (1), 113-132 (2020)
   PUBMED   32004755
  REMARK    GeneRIF: Netazepide Inhibits Expression of Pappalysin 2 in Type 1
            Gastric Neuroendocrine Tumors.
REFERENCE   6  (bases 1 to 8975)
  AUTHORS   Christians,J.K., Hoeflich,A. and Keightley,P.D.
  TITLE     PAPPA2, an enzyme that cleaves an insulin-like
            growth-factor-binding protein, is a candidate gene for a
            quantitative trait locus affecting body size in mice
  JOURNAL   Genetics 173 (3), 1547-1553 (2006)
   PUBMED   16702428
  REMARK    GeneRIF: The refinement of the target region to four genes and the
            finding that the QTL affects IGFBP-5 levels suggest that PAPPA2 may
            be involved with normal postnatal growth
REFERENCE   7  (bases 1 to 8975)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   8  (bases 1 to 8975)
  AUTHORS   Veal,E., Groisman,R., Eisenstein,M. and Gill,G.
  TITLE     The secreted glycoprotein CREG enhances differentiation of NTERA-2
            human embryonal carcinoma cells
  JOURNAL   Oncogene 19 (17), 2120-2128 (2000)
   PUBMED   10815803
REFERENCE   9  (bases 1 to 8975)
  AUTHORS   Veal,E., Eisenstein,M., Tseng,Z.H. and Gill,G.
  TITLE     A cellular repressor of E1A-stimulated genes that inhibits
            activation by E2F
  JOURNAL   Mol Cell Biol 18 (9), 5032-5041 (1998)
   PUBMED   9710587
REFERENCE   10 (bases 1 to 8975)
  AUTHORS   Rougeon,F., Chambraud,B., Foote,S., Panthier,J.J., Nageotte,R. and
            Corvol,P.
  TITLE     Molecular cloning of a mouse submaxillary gland renin cDNA fragment
  JOURNAL   Proc Natl Acad Sci U S A 78 (10), 6367-6371 (1981)
   PUBMED   6171818
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162173.4, AC115116.6 and AC158910.3.
            
            On Jun 20, 2019 this sequence version replaced NM_001085376.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164134
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AC162173.4         58136-58184
            50-1314             AC162173.4         58530-59794
            1315-2386           AC162173.4         79294-80365
            2387-2532           AC115116.6         187180-187325       c
            2533-2826           AC115116.6         172912-173205       c
            2827-3019           AC115116.6         170628-170820       c
            3020-3141           AC115116.6         166874-166995       c
            3142-3631           AC115116.6         163714-164203       c
            3632-3760           AC115116.6         160782-160910       c
            3761-3852           AC115116.6         154327-154418       c
            3853-4046           AC115116.6         150528-150721       c
            4047-4193           AC115116.6         148487-148633       c
            4194-4329           AC115116.6         130515-130650       c
            4330-4537           AC115116.6         130118-130325       c
            4538-4709           AC115116.6         103368-103539       c
            4710-4887           AC115116.6         99675-99852         c
            4888-5101           AC115116.6         97959-98172         c
            5102-5270           AC115116.6         80706-80874         c
            5271-5406           AC115116.6         78847-78982         c
            5407-5516           AC115116.6         77308-77417         c
            5517-5588           AC115116.6         66308-66379         c
            5589-5687           AC158910.3         163111-163209       c
            5688-8975           AC158910.3         157882-161169       c
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 68.49 cM"
     gene            1..8975
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /note="pappalysin 2"
                     /db_xref="GeneID:23850"
                     /db_xref="MGI:MGI:3051647"
     exon            1..49
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            50..1314
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     CDS             393..5762
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /note="pregnancy-associated plasma preproprotein-A2;
                     placenta-specific 3; pregnancy-associated plasma
                     protein-E"
                     /codon_start=1
                     /product="pappalysin-2 precursor"
                     /protein_id="NP_001078845.1"
                     /db_xref="CCDS:CCDS48409.1"
                     /db_xref="GeneID:23850"
                     /db_xref="MGI:MGI:3051647"
                     /translation="MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVL
                     LEGERCWLGYKVRRPRTAPQHHLFGVYPSRLENYPRSYPVGTQQQTLYAEWSQRSPER
                     QAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQLLRNDDTYLGEGGSKEALGE
                     PAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMTGDPQNTPQ
                     GFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLR
                     PEVLAEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGW
                     RDARFFFSLRTDRMKKATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQ
                     FGPLNSPFMASCRSLILGGDSSEDGHYFRGYLGTLVIWSTALSQTHLQHSPLHPSAAD
                     ELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPEILSPLQPPPCGQTACDNV
                     ELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFSRYNISW
                     QLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNR
                     RDGLCHVECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNST
                     HFLNVYFASSVREDLAGSATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVL
                     GLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCRDPEPANDTCGFTL
                     FPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSESQKPTPIPIPPMVIGQ
                     THKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYWTPEE
                     AVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVT
                     YLSMNSSKAFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERM
                     EIDAALLTSRPNSSLCSGCKPMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMY
                     QYQVQAEAVGELGEASPPLSHIHGGPYCGDGKVERSIGEMCDDGDLLNGDGCSRACEL
                     EEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLDQWASQAYSYHEDKE
                     KCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQKDN
                     EIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQ
                     QNPLVINVSHHVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNY
                     EGKSCAQRPCGEQGRCAPLLLDHTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLR
                     NMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLVNYANFSCLEGTDFLKQCSISCVPP
                     AKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFLEGNHDVGTICKY
                     ECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGF
                     KLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQG
                     YGIGAVCSPSCVIPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVH
                     CIQSCEPFQADGWCDTINNRAYCNYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPK
                     AEENQ"
     exon            1315..2386
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2532
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2826
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2827..3019
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3020..3141
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3142..3631
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3632..3760
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3761..3852
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3853..4046
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            4047..4193
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            4194..4329
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            4330..4537
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            4538..4709
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            4710..4887
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            4888..5101
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            5102..5270
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            5271..5406
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            5407..5516
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            5517..5588
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            5589..5687
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     exon            5688..8975
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8954..8959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /note="hexamer: ATTAAA"
     polyA_site      8975
                     /gene="Pappa2"
                     /gene_synonym="PAPP-A2; Pappe; PLAC3"
                     /note="major polyA site"
ORIGIN      
        1 agactggaag ctgaatggac acctagctga ggacctctga ggaaagaagt gaggagagga
       61 gttactgaag gaagaaaagg aaacctgagg tctctgggtg gagattcaaa gtaagaacac
      121 ttaacttcac tctgaaggac tgaaatcaag aagagaggtc acagaagaag gcaagagaag
      181 ccaaaagtta cagaagccac tggggttggc tgaatcccca ggacaggtag ccagtagagg
      241 catccttctg gggccacttg agaaagtttg gcttgggaat cgatttcccc ggtgacttca
      301 attccattgc tggttgtctt tccttcttct ccttattctt gtgtggtacc aagaagctga
      361 ctttgggttc taaggaacaa gctgagggag atatgatgtg ttggaaggtc ctgagaataa
      421 gcctggtggt cctgactggg tgggcactca gtaccaccaa ctcagagcag ggctggacac
      481 gcaagaaatt cctggcccag aaggaacacc tgaaccaagt gctgttggaa ggggaacgct
      541 gttggctggg gtataaggtt cgaagaccca ggactgctcc acagcaccat ctctttgggg
      601 tctacccaag ccgcctcgaa aactacccaa ggtcctatcc tgtggggaca cagcagcaaa
      661 ccctgtatgc agaatggagt cagcgaagcc cagagagaca ggctccgagt ggtattcccc
      721 gagacctgac tgaaaaccca gcaggagcaa ggagagagtc tcagcagcca gctgccccgt
      781 gggtagggga tggtcctatt gggcaatcgc agctgctgag aaatgatgac acttatcttg
      841 gcgagggagg atctaaggag gctctaggtg agcctgcgat tcagggaagc tcagaaattg
      901 ctgtctccac cattgctacc ttcgcatatc agaagggacc cacagcagag acccagagga
      961 agggccagtc caagtctagg cttcctcgcc aagtgcagaa gaggcaggca gaagacatga
     1021 ctggagaccc ccagaatacc cctcaaggtt tccagctttg gcctaaagag ccacttaagc
     1081 atggagatag tgacagtcca ttggagggca ccatccaaaa tggtggaggg gcctctctcc
     1141 ggagagtaga gacctttaac tcccaaggag gattgcctgt actgtatttc actgggaaga
     1201 gggagcggct gttgctgcgt ccagaagtgc tggctgagat tccccgggag gcgttcacag
     1261 tggaagcctg ggtgagacca gagggaggac agagcaaccc agccatcatt gcaggtgtgt
     1321 ttgataactg ctcccacaca gtcaatgaca agggctgggc cctggggatc cgctcaggga
     1381 aggacaaggg gtggagagat gctcgcttct tcttttccct ccgcactgac cggatgaaga
     1441 aagccactat tgtgactggc cacagccgtt accagccagg catgtggact catgtagcag
     1501 ccacttacga tggacagcat acagctctgt atgtggatgg cactcgagtg gctagtagtc
     1561 gtgaccagtt tggaccgcta aatagccctt tcatggcgtc ttgccgctct ttaatcctgg
     1621 gaggggacag ctccgaggat ggacattatt tccgtggtta cctgggcaca ctggtaatct
     1681 ggtcgaccgc cctttcacaa acccacctcc agcacagtcc cctgcatcca agtgcagcag
     1741 atgagttgac caccctgatc ctgacagcca ctttcgatcc tctgatggaa cagtgggctc
     1801 catttagaga tgacttatat ccccggctgg aggttctcca ggactctgag tcacagcctg
     1861 agattctgtc acctttacaa cccccacctt gtgggcaaac agcttgtgac aatgtggaac
     1921 tcatctccca gtacaataag catgggcccc ttcgtagaga gaaagtgatc cgctaccagg
     1981 tggtaaacat ctatgatgat gagggtctgc atcccattgt gagtgatcat cagatccgcc
     2041 gtcagcatga ggccctcaac caggccttca gccgctacaa tatcagctgg cagctgagtg
     2101 tccaccgagt ccacaactcc accctgcgcc accgggttgt gcttgttaat tgtgagccca
     2161 gcaagattgg caatgaccac tgtgaccctg aatgtgagca tccactcacc ggcttcgatg
     2221 ggggcgattg ccgcctgcag ggacgctgct actcctggaa tcgcagggat ggcctctgtc
     2281 acgtggagtg caacaacatg ctgaatgact tcgatgatgg agactgctgc gatccggaag
     2341 tgactgatgt ccgaaagact tgctttgatc ctgactcacc caagagggca tacatgagtg
     2401 tgaaggaact aaaggaggcc ttgcagctca acagcactca cttccttaat gtctacttcg
     2461 ccagctcagt ccgggaagac cttgcaggtt ctgccacatg gccctgggac aaggaagctc
     2521 tcagtcacct gggtggtgtt gtcctcaacc caacctatta tggcatgctt ggccacacca
     2581 acatcatgat ccatgaggtg ggacatgtcc tgggactcta ccatgtattc aaaggagtca
     2641 gtgaaagaga atcctgtgat gatccctgta gagagacagt gccatccatg gagacaggag
     2701 acctgtgtgc tgacactgct cccacaccca agagcaagct gtgtcgggac ccagagccag
     2761 ctaatgacac ctgtggcttc accctcttcc caggggctcc gttcaataac tacatgagct
     2821 acacagatga tgagtgcact gacaacttca cccctaacca agtggcccgg atgcattgct
     2881 acttggacct cgtataccag caatggagtg aaagccaaaa acctaccccc atacccatac
     2941 cacccatggt tattgggcaa acccacaagt cccttactat ccattggctg cctccaatca
     3001 gtggagttgt gtatgatagg gcccctgaca gcatgtgtag tgcctgcact gaagatggga
     3061 cattccgtca gtatgtatac aaggcatcct ctgggcgggt gtgtgactct tcaggttact
     3121 ggactccaga ggaggctgtg gggcctcctg atgtcgatca accctgtgag cctagcttgc
     3181 aggcctggag tcctgagctc cacctaaacc acatgaatat gaccgtgccc tgcccagcag
     3241 aaggctgtag cttggagttg ctttttcaat acccggtcca ggcggatacc ctcaccctct
     3301 gggtcactta cctctccatg aactcttcca aggcattctt tgacatagag atcttgctag
     3361 aacacaaaaa atctatgcac cttggacctt taaacacttt ttgtgacata ccgctcacca
     3421 tcaaactcca catggatgag aaggtcatgg gagcgaaagt ctacaccttt gatgaacgaa
     3481 tggaaattga tgctgctctc ctgacctctc ggcccaatag ttccttgtgt tctggatgca
     3541 agcctatgag ttaccaggtt cttcgagagc ctcctttttc cagtggcttg cccatggtgg
     3601 tgacacatcc acacagaaag ttcacagata cggaggtcat ccctgggcag atgtatcagt
     3661 accaagttca agctgaagct gtaggggaac tcggagaagc ttcacctcct ctgagccaca
     3721 ttcacggagg cccttactgt ggagatggga aggtagaaag gagtatcgga gagatgtgtg
     3781 atgatggaga cctgttgaat ggagatgggt gctcaagagc atgtgaactg gaggaaggct
     3841 tcaattgtgc aggggagcca agcctttgct acaggtatga gggagatggg gtttgtgaag
     3901 attttgagaa ggaaagcatc atcacagact gtggcctcca cactcctgaa ggatatttgg
     3961 atcagtgggc tagccaggct tattcctacc atgaagacaa ggagaaatgt cctgtttccc
     4021 tggtaacagg agaacctcat tcaatgatct gtacttcatg gcacccggat tcatccccat
     4081 atcattcctt tcctggctgg tttccctgtg tcttcagtct gaagaaagac caggatgcag
     4141 gcagtgagca ggctaaagat agcctgcaga aggacaatga aatttggctt gaagtgtgtt
     4201 tcaataggcc aggggttgca gtggcaattt acattttctt ggcatcggat ggcgtgaccc
     4261 ctggggagca tcagcaggca acagtgaccc tttacctgat tgatgtcagt ggaagcaacc
     4321 actctcttgg aacctatgga ctgtcgtgtc aacaaaatcc actggttatc aatgtgagcc
     4381 atcatgtgaa tgtctacccc caacatacct cctccatgct gctgaatttt tcatccccac
     4441 tggtgggcat ctcagcagtg gctctaagga catcctccca gaccagttct tcagctccca
     4501 gtaactgcat cccagagcaa aattatgagg gaaagagctg tgctcaacga ccttgtggag
     4561 aacagggaag atgtgcgcca ttgctactcg atcatacgga catggtgaat tgtacctcca
     4621 gtagcccagg tcacatggag tgcgctatca cttgtcaaag ggggtatgtc cttcagacca
     4681 gcagtgggca gtacctgagg aacatgcaga aagagattct ccttacttgt tcctctggcc
     4741 actgggacaa ggatgtgatc tgcaagcgtc ttgactgtgg tgtcccagac tcctccttgg
     4801 tgaattatgc aaacttctcc tgcttggagg gtacagactt tctgaaacaa tgttccatct
     4861 cgtgtgtccc accggctaag ctccaaggac tgaacccatg gctgacatgt cttgaagatg
     4921 gactctggtc tctccctgaa gtctactgca agttggaatg tgaagctcct ccagttattc
     4981 ccaatgccaa tctgctcctg ccacatttcc tggaaggcaa ccatgatgtg ggcaccatct
     5041 gcaaatatga gtgcaaacca ggctactatg tgaaggagac ttcaggaagt caaggcaaga
     5101 acaagttcct gaagatccag tgcctggatg atggaagctg ggaacaaggg agctgcgtcc
     5161 ccgtggtttg tgagcctcct cctccggtgt ttgaaggcat gtacgaatgt actgatggct
     5221 tcaagctgga cagccagtgt gtgctcaact gtaaccagga aacggaaggg attcccatcc
     5281 tctgcactaa agaggggttg tggacccagg aattcaagtt gtgtgaaaac ctgcaaggag
     5341 aatgcccacc acccacctca gagctgaatt ctgtggagta caagtgtgga cagggatatg
     5401 ggatcggtgc agtgtgctcc ccatcatgtg taatcccccc tagtgaccct gtgatactac
     5461 cagagaatgt gactactgac actttggagc actggatgga acctgtcaaa gtccagagta
     5521 tcgtgtgtac cggcaggcgc cagtggcacc cagatccctc tctggtgcac tgcatccagt
     5581 catgtgagcc tttccaagca gatggttggt gtgacactat taataaccgg gcctactgca
     5641 actatgacgg gggagactgc tgctcttcca cactctcttc caagaaggtc attccatttg
     5701 ctgctgattg tgacttggat gaatgcacct gtcgagaccc caaggcagaa gaaaaccagt
     5761 aactctagga gcagagccca tctcctctgc cctgggggca gactaaaaga gaagctcccg
     5821 tagcaggatg caaagggtga atgaacaaga taggttccat ggtggaagga gagcctaaaa
     5881 gagagccttt aagaaatgca agaaatgttt gtaggtgcca attaatgcat taagtagtcc
     5941 aagtagggag ggattagagg caattgtttc tgtaaaaaga cacttggtta gaaatggaag
     6001 gagaaattga cagacccctc cccccatctg tactctgctc tatccttctc tcaatctcct
     6061 ccccactctg cacgctgtca gtggctcagc cccacccaac atgtaaaccc atatcaaaat
     6121 attagaagat aagctgccag ggactccgtc aatacccatt tcagatggac tgtcatcttt
     6181 cagggctcat ctattccaga ctggcccttt gccttccttt tgtgtggtct ctctcaaata
     6241 aggaggttag ctatttcttt ataagtattt aaacataatt atataaatat attatatatt
     6301 atattttttg ctgtttacta agctacagat tatccattgt tccacaaatg ctgctgtgaa
     6361 gttcatgtcc agagggatac tgaagcttcg aagacagaaa gaaaggttcc tttgccttct
     6421 tcccatagat agggattggc aggtcggatg gggagaagag agtcgatgga attctggatg
     6481 ctagcctgtc agctgatcat cattttatga cctgggagct gggcctactg aaaggagata
     6541 gacttggcat gctaagagca agaggcatgg aaagttacat gggaacctat ttggcagtag
     6601 gtccaggaga aatagacaat ggtttctaag tatctaccaa aaggagcata caaactcttc
     6661 tacgcttctc agaactctca gttggagctg gtaaagacca gggtcttttc cacttctcca
     6721 tgtacttagt ccaagtatag ctgccagcca gccaaccaac cagccagcca gccagccagc
     6781 cagccagcca gccaaccagc cagccaaaaa gtttgtatga gggctcattc tgtgaacatc
     6841 aggcttacag gaacttacac cctcttcata cttggccagt tgctgtctct ccttgttgca
     6901 agtctcaagg tctggccaat tgttcacagc cacatcacta actgttgatt tttctgagaa
     6961 aagaaaatgc tttggagatt tggaaatgtg ggggaggaga taagagatga aagaacgggg
     7021 agttagaaga gcgaaaggct gtcagagagg tgaaaggtca gagaggtgag aggtcaagat
     7081 taaatgaggt ctgaggataa aaggatagaa tgagtgaaca agcccctgat ccaagtctcc
     7141 aaaagaggcc agggatcctt cgagcaaaga gataaatctt attcaagtgc aaaatctgaa
     7201 gtttgtggcc cagtaagcaa catgtggagc gagggtgaca ctctgtgggg agaaagaaga
     7261 atttcaacta tttagggtcc atgctctctt taccatttct tgttcaatag ctgtatggac
     7321 tatgcacaaa acagtcacat taacacttct ctgtttatcc agactattct catacttttc
     7381 tataggctct gtccttggta tgttttctta tagcagtttc tagatcacac ctagaaagag
     7441 agtcaggaca tagtttcttt cctttaatga cggtcttgtt ctcttacaga gaccctagtt
     7501 ttttcctcca gtggaaacta agaccaagca aaacttggag tcacttttgg tatctccaaa
     7561 acctctcctc ctcttccttc tactcttttg tcatcaagac tgaagcaact aatctataga
     7621 atgagaaagg aaactttttt ttatcatcat aagcacaatt atccctgctc tatatgaaga
     7681 gcaatattct gcagtgactg gatatggcaa tatacataca gttacctatg aatatgtgga
     7741 tcatggacaa caaagccatt gagaagggga aaggcagtgt tagtcttcta ctcctagcaa
     7801 taaggtaaac agtgcttaaa tactgtttac tatctaggtc acacttatta ccctttgtca
     7861 ggaaatacca ttcatattct gaatctcata agtttaatat ggaaagaaaa atagaaaagt
     7921 ttaggttctc tactacatat ttcaattcct atttctgtat ctcattaagg tgagtatgaa
     7981 aagaccaata gaaaaattta aattctctac taaacacaca cacacacaca cacacacaca
     8041 cacacacaca ctgtttcctt agagaacatt caaattcctg gatgtgtgtc aacttttgcc
     8101 ttaggaaaat cctgacagag acagagcaat agttaccttg ggcacaactt agtgcacaga
     8161 gactgccatt cccaagagca ctcatgggca cagactaccc accagtatct gtccatgctc
     8221 agtctcctca ttaagtccac taagactaac agagacccca tgcttcactt taagatactt
     8281 tgttcttttt ggcaacattc accctgccct gggaaggcaa gaggacagtt tctggggtgt
     8341 gtgtaaagct ccccaggctg aaggccattt agccatcatc agccagagac agagacggga
     8401 gtcttatcta gactttgtcc cttggctcca gtatttgaag aaacttaagt ccctaataaa
     8461 gaaaatgatc agagatccta ggaacaagtc atgtttgccc ccaggtggtt gtattcagtt
     8521 ctccttagac atccataatg tcatccaggg ccctccacag aggctcctct gcctgtttct
     8581 ttattacaaa gttacctgtc tatttctagt tcctggtaac cacagttttg agatcaacat
     8641 ggcccaaagg tggctggctt caatagtagc atggcagcct tcaatcacag atacagaaaa
     8701 gagatcagca ggggcaaaga gcagatctgg gcaaataagg cagcgagtag agtcttcttg
     8761 gttcttttct ttcctttcca acatttaagg ccttagtctt tgacacatca tctattctgt
     8821 gtgatcacca ttgagtgtgt cagttagcac ccaaggggac agtttgatgg caaacacagt
     8881 gaaaggggct gatctactgt attgtaatgt aaaacagcta catccagtta ttttataaga
     8941 ttataaaacg ttcattaaaa aatcagcaca taaaa
//
