LOCUS       NM_019943               2337 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus poly (A) polymerase beta (testis specific) (Papolb),
            mRNA.
ACCESSION   NM_019943
VERSION     NM_019943.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Kashiwabara,S.I., Tsuruta,S., Yamaoka,Y., Oyama,K., Iwazaki,C. and
            Baba,T.
  TITLE     PAPOLB/TPAP regulates spermiogenesis independently of chromatoid
            body-associated factors
  JOURNAL   J Reprod Dev 64 (1), 25-31 (2018)
   PUBMED   29109362
  REMARK    GeneRIF: While PIWIL1 and YBX2 were associated with translationally
            inactive messenger ribonucleoproteins and translating
            polyribosomes, PAPOLB was present almost exclusively in the
            mRNA-free fractions of sucrose gradients. These results suggest
            that PAPOLB may regulate spermiogenesis through a pathway distinct
            from that mediated by chromatoid body-associated factors.
REFERENCE   3  (bases 1 to 2337)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2337)
  AUTHORS   Auclair,G., Guibert,S., Bender,A. and Weber,M.
  TITLE     Ontogeny of CpG island methylation and specificity of DNMT3
            methyltransferases during embryonic development in the mouse
  JOURNAL   Genome Biol 15 (12), 545 (2014)
   PUBMED   25476147
REFERENCE   5  (bases 1 to 2337)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   6  (bases 1 to 2337)
  AUTHORS   Kashiwabara,S., Noguchi,J., Zhuang,T., Ohmura,K., Honda,A.,
            Sugiura,S., Miyamoto,K., Takahashi,S., Inoue,K., Ogura,A. and
            Baba,T.
  TITLE     Regulation of spermatogenesis by testis-specific, cytoplasmic
            poly(A) polymerase TPAP
  JOURNAL   Science 298 (5600), 1999-2002 (2002)
   PUBMED   12471261
  REMARK    GeneRIF: propose that TPAP governs germ cell morphogenesis by
            modulating specific transcription factors at posttranscriptional
            and posttranslational levels
REFERENCE   7  (bases 1 to 2337)
  AUTHORS   Murata,T., Nagaso,H., Kashiwabara,S., Baba,T., Okano,H. and
            Yokoyama,K.K.
  TITLE     The hiiragi gene encodes a poly(A) polymerase, which controls the
            formation of the wing margin in Drosophila melanogaster
  JOURNAL   Dev Biol 233 (1), 137-147 (2001)
   PUBMED   11319863
REFERENCE   8  (bases 1 to 2337)
  AUTHORS   Lee,Y.J., Lee,Y. and Chung,J.H.
  TITLE     An intronless gene encoding a poly(A) polymerase is specifically
            expressed in testis
  JOURNAL   FEBS Lett 487 (2), 287-292 (2000)
   PUBMED   11150526
REFERENCE   9  (bases 1 to 2337)
  AUTHORS   Kashiwabara,S., Zhuang,T., Yamagata,K., Noguchi,J., Fukamizu,A. and
            Baba,T.
  TITLE     Identification of a novel isoform of poly(A) polymerase, TPAP,
            specifically present in the cytoplasm of spermatogenic cells
  JOURNAL   Dev Biol 228 (1), 106-115 (2000)
   PUBMED   11087630
REFERENCE   10 (bases 1 to 2337)
  AUTHORS   Zhao,W. and Manley,J.L.
  TITLE     Complex alternative RNA processing generates an unexpected
            diversity of poly(A) polymerase isoforms
  JOURNAL   Mol Cell Biol 16 (5), 2378-2386 (1996)
   PUBMED   8628305
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CAAA01187077.1.
            
            On Oct 21, 2005 this sequence version replaced NM_019943.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AB030729.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2337              CAAA01187077.1     11327-13663         c
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 81.53 cM"
     gene            1..2337
                     /gene="Papolb"
                     /gene_synonym="PAP; Paplob; Papola-ps; Papt; Plap-ps;
                     Tpap"
                     /note="poly (A) polymerase beta (testis specific)"
                     /db_xref="GeneID:56522"
                     /db_xref="MGI:MGI:1932115"
     exon            1..2337
                     /gene="Papolb"
                     /gene_synonym="PAP; Paplob; Papola-ps; Papt; Plap-ps;
                     Tpap"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..2119
                     /gene="Papolb"
                     /gene_synonym="PAP; Paplob; Papola-ps; Papt; Plap-ps;
                     Tpap"
                     /EC_number="2.7.7.19"
                     /note="PAP-beta; polynucleotide adenylyltransferase beta;
                     testis-specific poly(A) polymerase"
                     /codon_start=1
                     /product="poly(A) polymerase beta"
                     /protein_id="NP_064327.1"
                     /db_xref="CCDS:CCDS39362.1"
                     /db_xref="GeneID:56522"
                     /db_xref="MGI:MGI:1932115"
                     /translation="MMPFAVTTQGAQQPAPAPKQFGISSPISLAAPKDTDRELTQKLI
                     ETLQPFGVFEEEEELQRRILILQKLNNLVKEWIREISESRNLPQAVIENVGGKIFTFG
                     SYRLGVHTKGADIDALCVAPRHVDRNDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKL
                     CFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNI
                     DSFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVRKFFLV
                     FSEWEWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVST
                     RMVMIEEFKQGLAITHEILLNKAEWSKLFEAPSFFQKYKHYIVLLASAPTEKQHLEWV
                     GLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKETADKEEFRTMWVIGLVLKKPENS
                     EILSIDLTYDIQSFTDTVYRQAINSKMFEMDMKIAAMHLRRKELHQLLPNHVLQKKET
                     HLTESVRLTAVTDSSLLLSIDSENSMTAPSPTGTMKTGPLTGNPQGRNSPALAVMAAS
                     VTNIQFPDVSLQHVNPIESSGIALSESIPQIPSQPTISPPPKPTMTRVVSSTHLVNHP
                     SRPSGNTATNIPNPILGV"
     regulatory      2309..2314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Papolb"
                     /gene_synonym="PAP; Paplob; Papola-ps; Papt; Plap-ps;
                     Tpap"
                     /note="hexamer: AATAAA"
     polyA_site      2337
                     /gene="Papolb"
                     /gene_synonym="PAP; Paplob; Papola-ps; Papt; Plap-ps;
                     Tpap"
                     /note="major polyA site"
ORIGIN      
        1 ggagcccggc gccatgttgg cccggaggga taccagggga agcgcttgag gcggcgggct
       61 cggagcggag cgggggccga cagggccggc cggcaggagc ccgcccctcg cgccttcgga
      121 tcatgagccg agcagaggcc gtcgaggtcg cggtggcggg gcaggtgccc tggcggcgcg
      181 ggagctagag atgatgccat ttgcggtgac cacccaagga gcacaacaac cggctcccgc
      241 cccgaagcag ttcggcatat cgtcccccat cagcctggcc gcccccaagg acacagaccg
      301 cgaactcacc cagaagctga tcgagaccct ccagcccttc ggggtgtttg aagaggaaga
      361 ggaactgcag cgcaggattt taattttgca gaaattaaat aatctggtga aggaatggat
      421 ccgagaaatc agtgaaagca ggaatcttcc acaagctgta attgagaatg ttggggggaa
      481 aatttttacg ttcggctctt accgattagg agtacacacg aaaggtgcag atatcgatgc
      541 tttgtgcgtt gcaccaagac acgtggatcg aaatgacttt ttcacctcct tctatgacaa
      601 attgaaacta caagaggaag taaaagattt aagagctgtt gaggaggcct ttgtgccggt
      661 tatcaaactg tgttttgatg gaatagagat tgatattttg tttgcaagat tagcattgca
      721 gactattcca gaagatttgg acctacgaga tgatagtctg cttaaaaatt tagatattag
      781 gtgcataaga agccttaacg gttgccgggt aactgatgag attttacatc tagtaccaaa
      841 cattgacagc ttcaggttaa cactgagagc catcaaatta tgggccaaat gccacaacat
      901 ctattccaat atattgggtt tccttggagg tgtttcctgg gctatgctag tagcaagaac
      961 ttgccagctt tacccaaatg caatagcatc cactcttgtg cgaaaatttt tcttggtgtt
     1021 ttctgagtgg gaatggccaa atccagtgtt gttaaaagaa ccagaagaac gaaatcttaa
     1081 cttgcctgta tgggacccaa gagtgaatcc tagtgacagg taccatctta tgcctataat
     1141 tacaccagca tacccacagc agaactccac gtacaatgtg tctgtttcga ccaggatggt
     1201 catgattgag gagtttaaac aaggacttgc tatcacacat gaaattctgc tgaataaggc
     1261 agaatggtcc aaactttttg aagctccaag cttttttcaa aaatacaagc attatattgt
     1321 acttctggca agtgcgccaa cagagaaaca acatttagag tgggtgggct tggtggaatc
     1381 aaagatccga atcctggttg ggagcctgga aaagaatgag ttcattactc tggctcatgt
     1441 gaatcctcag tcgtttccag caccaaaaga aactgctgac aaggaagaat ttcgtactat
     1501 gtgggtgatc gggttagtgt tgaagaagcc agaaaactct gaaattctca gtattgatct
     1561 cacctatgat attcagtctt tcacagatac agtgtacaga caagcaataa atagtaagat
     1621 gtttgagatg gatatgaaga ttgctgcaat gcatctaaga aggaaggaac ttcatcagct
     1681 acttccaaat catgttcttc agaaaaagga aacccattta acagaaagtg taaggctgac
     1741 agctgtgact gacagcagcc ttctcttgtc tatagacagt gaaaacagca tgactgctcc
     1801 ttcacctact ggcactatga agacaggtcc actgactgga aaccctcagg gcagaaacag
     1861 tcctgctctg gctgttatgg cagcatctgt gaccaacata cagtttcctg atgtttcctt
     1921 gcagcatgtg aatcccatag aaagctcagg aattgcactg agtgaaagca tccctcagat
     1981 tccctcacaa cctaccattt caccaccacc taagcctaca atgaccagag tggtttcttc
     2041 gacacatctg gtaaaccatc catctagacc ttcaggaaat acagcaacga acatacccaa
     2101 tcctatacta ggagtctaga agacagtctc agctgataag aagaaagaaa caagaaaccc
     2161 aacagaaggg aagaaccaga tagatgagtc ccgcttagac accactcaga cagcagcttc
     2221 tctgttggcc tctcaggaaa cacctgggaa cagataagcc cggtatccct gtttttccta
     2281 gagatcctat tccttttatt gagagttcaa taaaacagat tgaatggata gaaacta
//
