LOCUS       NM_001347440            4460 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus poly (A) polymerase alpha (Papola), transcript variant
            2, mRNA.
ACCESSION   NM_001347440 XM_017314993
VERSION     NM_001347440.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   3  (bases 1 to 4460)
  AUTHORS   Kiefer,H., Mizutani,A., Iemura,S., Natsume,T., Ando,H., Kuroda,Y.
            and Mikoshiba,K.
  TITLE     Inositol 1,4,5-triphosphate receptor-binding protein released with
            inositol 1,4,5-triphosphate (IRBIT) associates with components of
            the mRNA 3' processing machinery in a phosphorylation-dependent
            manner and inhibits polyadenylation
  JOURNAL   J Biol Chem 284 (16), 10694-10705 (2009)
   PUBMED   19224921
REFERENCE   4  (bases 1 to 4460)
  AUTHORS   Lee,S.H., Choi,H.S., Kim,H. and Lee,Y.
  TITLE     ERK is a novel regulatory kinase for poly(A) polymerase
  JOURNAL   Nucleic Acids Res 36 (3), 803-813 (2008)
   PUBMED   18084034
REFERENCE   5  (bases 1 to 4460)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   6  (bases 1 to 4460)
  AUTHORS   Kashiwabara,S., Noguchi,J., Zhuang,T., Ohmura,K., Honda,A.,
            Sugiura,S., Miyamoto,K., Takahashi,S., Inoue,K., Ogura,A. and
            Baba,T.
  TITLE     Regulation of spermatogenesis by testis-specific, cytoplasmic
            poly(A) polymerase TPAP
  JOURNAL   Science 298 (5600), 1999-2002 (2002)
   PUBMED   12471261
REFERENCE   7  (bases 1 to 4460)
  AUTHORS   Kim,H. and Lee,Y.
  TITLE     Interaction of poly(A) polymerase with the 25-kDa subunit of
            cleavage factor I
  JOURNAL   Biochem Biophys Res Commun 289 (2), 513-518 (2001)
   PUBMED   11716503
REFERENCE   8  (bases 1 to 4460)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 4460)
  AUTHORS   Hwang,S.Y., Oh,B., Knowles,B.B., Solter,D. and Lee,J.S.
  TITLE     Expression of genes involved in mammalian meiosis during the
            transition from egg to embryo
  JOURNAL   Mol Reprod Dev 59 (2), 144-158 (2001)
   PUBMED   11389549
REFERENCE   10 (bases 1 to 4460)
  AUTHORS   Zhao,W. and Manley,J.L.
  TITLE     Complex alternative RNA processing generates an unexpected
            diversity of poly(A) polymerase isoforms
  JOURNAL   Mol Cell Biol 16 (5), 2378-2386 (1996)
   PUBMED   8628305
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158526.3, BY308303.1, AK132704.1, BC060128.1, CF167932.1,
            BU056874.1 and CX200364.1.
            
            On Nov 29, 2016 this sequence version replaced XM_017314993.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region compared to variant 1. The encoded
            isoform (2) has the same N- and C-termini, but is shorter than
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.701371.1,
                                           SRR1660811.135746.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AC158526.3         52135-52139         c
            6-19                BY308303.1         1-14
            20-1538             AK132704.1         2-1520
            1539-1840           BC060128.1         1381-1682
            1841-2160           AK132704.1         1823-2142
            2161-2646           CF167932.1         1-486
            2647-3657           BC060128.1         2552-3562
            3658-4320           BU056874.1         38-700
            4321-4460           CX200364.1         376-515
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 56.06 cM"
     gene            1..4460
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /note="poly (A) polymerase alpha"
                     /db_xref="GeneID:18789"
                     /db_xref="MGI:MGI:109301"
     exon            1..218
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     CDS             211..2367
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /EC_number="2.7.7.19"
                     /note="isoform 2 is encoded by transcript variant 2;
                     PAP-alpha; polynucleotide adenylyltransferase"
                     /codon_start=1
                     /product="poly(A) polymerase alpha isoform 2"
                     /protein_id="NP_001334369.1"
                     /db_xref="CCDS:CCDS84000.1"
                     /db_xref="GeneID:18789"
                     /db_xref="MGI:MGI:109301"
                     /translation="MPFPVTTQGSQQTQPPQRHYGITSPISLAAPKETDCLLTQKLIE
                     TLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGS
                     YRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLC
                     FDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNID
                     NFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVF
                     SKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTR
                     MVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVG
                     LVESKIRILVGSLEKNEFITLAHVNPQSFPAPKESPDREEFRTMWVIGLVFKKTENSE
                     NLSVDLTYDIQSFTDTVYRQAINSKMFELDMKIAAMHVKRKQLHQLLPSHVLQKRKKH
                     STEGVKLTALNDSSLDLSMDSDNSMSVPSPTSAMKTSPLNSSGSSQGRNSPAPAVTAA
                     SVTSIQASEVSVPQANSSESPGGPSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNP
                     SPRPSGNTATKVPNPIVGVKRTSSPNKEESPKKTKTEEEQVDLETSAVQSETVPASAS
                     LLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR"
     exon            219..392
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            393..459
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            460..541
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            542..651
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            652..705
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            706..817
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            818..907
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1046
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1119
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1240
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1325
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1379
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1499
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1609
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1731
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1874
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..1975
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2196
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2271
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..4451
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2438
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /note="major polyA site"
     regulatory      4196..4201
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
                     /note="hexamer: AATAAA"
     polyA_site      4218
                     /gene="Papola"
                     /gene_synonym="Pap; PapIII; Plap"
ORIGIN      
        1 ggttctagaa cgttgctgtg gtagcgctcg ggcgccatgt taggacgaag gggaaggagg
       61 agaagcgctt aaagcggcgg ggcacgggtg caggactggg ttggccccgg ggctgaggta
      121 ggctgccgtc cttcccgcct cggtggatca tgcccaggac ggcagcggtc gcggggggga
      181 agtgactggg cggtgccggc gccggagacg atgccgtttc cagttacaac gcagggatca
      241 caacaaacgc agccaccaca gaggcactat ggcattacct ctcctatcag cttagcggcc
      301 cccaaggaga ctgactgcct actcacacag aagctcatcg agacgctgaa gccctttggg
      361 gtttttgaag aagaagagga actgcagcgc aggattttaa ttttgggaaa attaaataac
      421 ctggtgaaag aatggattcg agaaatcagt gaaagcaaga atctcccaca atctgtaatt
      481 gaaaatgttg gagggaagat ttttacattt ggatcttaca gactaggagt ccacacgaaa
      541 ggtgctgata ttgatgcgtt gtgtgttgca ccaagacatg ttgatcgaag tgactttttc
      601 acctcattct atgataaatt gaaattacaa gaagaagtga aagatttaag agctgttgaa
      661 gaggcatttg taccagttat caaactctgt tttgatggaa tagagattga tattttgttt
      721 gcaagattag cactgcagac tattccagaa gatttggacc tacgagatga cagtctgctt
      781 aaaaacctag atataagatg cataagaagc cttaatggtt gcagggtaac cgatgaaatt
      841 ttacatctag taccaaacat tgacaacttc aggttaactc tgagagccat caaactgtgg
      901 gccaaacggc acaacatcta ttccaatata ttaggtttcc tcggtggtgt ttcctgggct
      961 atgctagtag caagaacttg ccagctttat ccaaatgcaa tagcatcaac tcttgtacat
     1021 aaatttttct tggtattttc taaatgggaa tggccaaatc cagtgctatt gaaacagcct
     1081 gaagaatgca atcttaattt gcctgtgtgg gacccaaggg taaaccccag tgataggtac
     1141 catcttatgc ctataattac accagcatac ccacagcaga actccacgta caatgtgtcc
     1201 gtttcaacac ggatggtcat ggttgaggag tttaaacaag gtcttgctat cacagatgaa
     1261 attttgctga gtaaggcaga gtggtccaaa ctttttgaag ctccaaactt ctttcagaag
     1321 tacaagcatt atattgtact tctagcaagt gcgcccacgg aaaagcagcg tctggaatgg
     1381 gtgggcttgg tggaatcaaa aatccgcatc ctggttggaa gcttggagaa gaatgagttt
     1441 attacactgg ctcatgtgaa tccccagtca tttccagccc ccaaagaaag tcctgacagg
     1501 gaagaatttc gcacaatgtg ggtgattggg ttagtgttta aaaaaactga aaactctgaa
     1561 aatctcagtg tcgacctcac ctatgatatc cagtctttca cagacacagt ttataggcaa
     1621 gcaataaaca gcaaaatgtt tgagttggat atgaagattg cagcaatgca tgtgaagaga
     1681 aagcaactcc atcagctgct gcctagtcac gtgcttcaga agaggaagaa gcattcaaca
     1741 gaaggagtca agttaacagc tctgaatgac agcagccttg acttgtctat ggacagtgat
     1801 aacagcatgt ctgtgccttc acccaccagt gctatgaaga ccagtccatt gaatagttct
     1861 ggcagctccc agggcagaaa cagtcctgct ccagctgtga ccgcagcatc tgtgaccagc
     1921 atccaggctt ctgaggtttc tgtaccgcaa gcaaattcca gtgaaagccc agggggtcca
     1981 tcgagcgaaa gcattcctca aactgccaca cagccagcca tttctccacc accaaagcct
     2041 acagtctcca gagttgtctc ctcaacacga ctggtaaacc catcgcctag accttcagga
     2101 aacacagcaa caaaagtccc taatcctata gtaggagtca agagaacgtc ctcacccaat
     2161 aaagaagaaa gtcctaagaa aaccaaaaca gaagaggaac aagttgatct ggagacaagt
     2221 gcggttcaat cagaaactgt tccggcatcg gcttctctgt tggcctctca gaaaacatcc
     2281 agtacagacc tttctgatat ccctgctctc cctgcaaatc ctattcctgt tatcaagaat
     2341 tcaataaaac tgagactgaa tcggtaaaac cacctcaggg tccataaaca gtatctgcca
     2401 actcaacccg ttgtctccag atgcttaaac aaaggaaaga aagaaagaaa ggaaaagagg
     2461 agattggagg gacaagacta gacctgactg acagtgggtt tagctgtttg gtggcctccc
     2521 tgccttgggc tcatgagcac ttgatcagaa gtccaggtta gtatgcgaag ccaggagtgc
     2581 tattattact gtgttagcga cagctgcatt aactactcag aaaaattact gcctttaaga
     2641 aggaaacaaa acaaaaccct cgagctatgt ttgtatccat aactgacatc cggatcgcgc
     2701 ctctgtgtgc tgcattgttt ggaaaactgc aatacaagct ggcagaagaa ctcctcattc
     2761 tgatgatgca cttttatgta ttttttttta attagaaagt agaactaatt ttagattttc
     2821 agcttaatgg attttcaatt ttttttcccc aaagaatttt ctttacaatt agtctttaaa
     2881 ttggatacgt tgtgcattgg tgtaccattc tgtcccagaa agggcaggag gacaccgagc
     2941 tcagcccagt ccttacgggc agctgtagcc cttagggtga attgtcaatg ctaaggtata
     3001 ctttggacac tgaaagaaga attaggcgat acttggtttt gatgaggtta tgattaagca
     3061 gtatatattc cttttattta tagagttgtt ttatagtgca tacaaatcag cattcggcca
     3121 acaagtgtgg tctttgagct ttcgcaagct ctgttctttt gccttcagtc tgttagtctt
     3181 cagaaagaca gtgctccccc accccctttt attttatttt ttgcctttct tttttcccct
     3241 ttgcttgtat gcacaaggta aggcttattt cacagaaacc agaatgccag tattttccta
     3301 agccatgaca tgtaaccttg accctgtggc tgcgtgacac agacactaac tttggtccca
     3361 tggctagaac ttgagagtga ctgaagccat acctgtgaag catccccgga ggcggccctt
     3421 tgatctctgc gaggactttt gtacactcca cacaactcct gttcctgtct tagacaaatt
     3481 gattttcatt tttaaaagtg ggcaaaaggc attttcttcc agatttttaa actaattttt
     3541 atttttaacc agtttatcaa aatttgtcag tgaattttac atacaaaaac attttaaaaa
     3601 tcatttctag taagcacttg acatttagtc agctttccac ttttgaattt ttttttttgt
     3661 tctatcaaaa attagaaatc ttctttaaag acaatgattt ctcataatta agtagcagaa
     3721 ttctcgtcca tcttgtgaca tctgacgggt gccagatgtt gataaagtta ctctttgtta
     3781 atctgggtaa aacagacctg tagcagcaga tctttgtatg agcatggttt ttgaatcctt
     3841 catttcgctg cattctatca cacgtttgtg cattttcatg ttacactgca attacttaaa
     3901 ctctcccctg tccttccaaa ttaatttcca aagttggagt atagtctttc ccctgaagct
     3961 atgcattaat taaggctttc tttaccactt acattgttta tatacagtgt tctactctcc
     4021 acctcttgct ctgcagtcac cttgcccttc ctcccaccat ttgagtaaag aagacggtcc
     4081 gctaacaggt gacatgtact ggtgtctgtg tgtgtagctt cagagttaga ttgacatcac
     4141 caggcacaga tttagtcttg tcgttttgtt tacacattgg ggaaaaattc agtttaataa
     4201 acgttccatg taactgcatc caagtttcac caggctggag acgtggatct tttctgtgta
     4261 gtgacttttt acttctagtt tccataacct ggagatcaga ctgttgcttt cgcatgatgt
     4321 aagtagtgtc tcatgactgg agtttgcttt gttttatagt atctgtactc cttgtatttt
     4381 tcaagagcta ttttgtaaac agatgatgta tttctccatt gaaaacacaa taaaaaaaaa
     4441 aacagcacaa aaaaaaaaaa
//
