LOCUS       NM_153783               1737 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus polyamine oxidase (exo-N4-amino) (Paox), transcript
            variant 1, mRNA.
ACCESSION   NM_153783
VERSION     NM_153783.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 1737)
  AUTHORS   Tormos,J.R., Henderson Pozzi,M. and Fitzpatrick,P.F.
  TITLE     Mechanistic studies of the role of a conserved histidine in a
            mammalian polyamine oxidase
  JOURNAL   Arch Biochem Biophys 528 (1), 45-49 (2012)
   PUBMED   22959971
  REMARK    GeneRIF: Steady-state kinetics of His64 mutant proteins shows that
            the mutations result in a decrease of ~15-fold in the rate constant
            for amine oxidation.
REFERENCE   4  (bases 1 to 1737)
  AUTHORS   Henderson Pozzi,M. and Fitzpatrick,P.F.
  TITLE     A lysine conserved in the monoamine oxidase family is involved in
            oxidation of the reduced flavin in mouse polyamine oxidase
  JOURNAL   Arch Biochem Biophys 498 (2), 83-88 (2010)
   PUBMED   20417173
REFERENCE   5  (bases 1 to 1737)
  AUTHORS   Pozzi,M.H., Gawandi,V. and Fitzpatrick,P.F.
  TITLE     Mechanistic studies of para-substituted
            N,N'-dibenzyl-1,4-diaminobutanes as substrates for a mammalian
            polyamine oxidase
  JOURNAL   Biochemistry 48 (51), 12305-12313 (2009)
   PUBMED   19911805
  REMARK    GeneRIF: N,N'-dibenzyl-1,4-diaminobutanes, with only two nitrogens,
            are also substrates of polyamine oxidase.
REFERENCE   6  (bases 1 to 1737)
  AUTHORS   Bianchi,M., Polticelli,F., Ascenzi,P., Botta,M., Federico,R.,
            Mariottini,P. and Cona,A.
  TITLE     Inhibition of polyamine and spermine oxidases by polyamine
            analogues
  JOURNAL   FEBS J 273 (6), 1115-1123 (2006)
   PUBMED   16519678
REFERENCE   7  (bases 1 to 1737)
  AUTHORS   Royo,M. and Fitzpatrick,P.F.
  TITLE     Mechanistic studies of mouse polyamine oxidase with
            N1,N12-bisethylspermine as a substrate
  JOURNAL   Biochemistry 44 (18), 7079-7084 (2005)
   PUBMED   15865452
REFERENCE   8  (bases 1 to 1737)
  AUTHORS   Chaturvedi,R., Cheng,Y., Asim,M., Bussiere,F.I., Xu,H.,
            Gobert,A.P., Hacker,A., Casero,R.A. Jr. and Wilson,K.T.
  TITLE     Induction of polyamine oxidase 1 by Helicobacter pylori causes
            macrophage apoptosis by hydrogen peroxide release and mitochondrial
            membrane depolarization
  JOURNAL   J Biol Chem 279 (38), 40161-40173 (2004)
   PUBMED   15247269
  REMARK    GeneRIF: new mechanism for pathogen-induced oxidative stress in
            macrophages in which activation of PAO1 leads to H(2)O(2) release
            and apoptosis by a mitochondrial-dependent cell death pathway
REFERENCE   9  (bases 1 to 1737)
  AUTHORS   Wu,T., Yankovskaya,V. and McIntire,W.S.
  TITLE     Cloning, sequencing, and heterologous expression of the murine
            peroxisomal flavoprotein, N1-acetylated polyamine oxidase
  JOURNAL   J Biol Chem 278 (23), 20514-20525 (2003)
   PUBMED   12660232
  REMARK    GeneRIF: translated amino acid sequences of mpao and bpao have a
            -Pro-Arg-Leu peroxisomal targeting signal at the extreme C termini
            and a beta-alpha-beta FAD-binding motif is present in the
            N-terminal portion of mPAO.
REFERENCE   10 (bases 1 to 1737)
  AUTHORS   Vujcic,S., Liang,P., Diegelman,P., Kramer,D.L. and Porter,C.W.
  TITLE     Genomic identification and biochemical characterization of the
            mammalian polyamine oxidase involved in polyamine back-conversion
  JOURNAL   Biochem J 370 (Pt 1), 19-28 (2003)
   PUBMED   12477380
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC109205.12.
            
            On May 1, 2007 this sequence version replaced NM_153783.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC082783.1, AK030664.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               AC109205.12        135875-136072
            199-688             AC109205.12        136516-137005
            689-888             AC109205.12        137698-137897
            889-1141            AC109205.12        139208-139460
            1142-1254           AC109205.12        141837-141949
            1255-1412           AC109205.12        142571-142728
            1413-1737           AC109205.12        144200-144524
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 85.14 cM"
     gene            1..1737
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /note="polyamine oxidase (exo-N4-amino)"
                     /db_xref="GeneID:212503"
                     /db_xref="MGI:MGI:1916983"
     exon            1..198
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1556
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /EC_number="1.5.3.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     peroxisomal N1-acetyl-spermine/spermidine oxidase;
                     peroxisomal N(1)-acetyl-spermine/spermidine oxidase;
                     exo-N4-amino"
                     /codon_start=1
                     /product="peroxisomal N(1)-acetyl-spermine/spermidine
                     oxidase isoform 1"
                     /protein_id="NP_722478.2"
                     /db_xref="CCDS:CCDS21965.1"
                     /db_xref="GeneID:212503"
                     /db_xref="MGI:MGI:1916983"
                     /translation="MAFPGPRVLVVGSGIAGLGAAQKLCSHRAAPHLRVLEATASAGG
                     RIRSERCFGGVVELGAHWIHGPSQDNPVFQLAAEFGLLGEKELSEENQLVDTGGHVAL
                     PSMIWSSSGTSVSLELMTEMARLFYGLIERTREFLNESETPMASVGEFLKKEISQQVA
                     SWTEDDEDTRKRKLAILNTFFNIECCVSGTHSMDLVALAPFGEYTVLPGLDCILAGGY
                     QGLTDRILASLPKDTVAFDKPVKTIHWNGSFQEAAFPGETFPVLVECEDGARLPAHHV
                     IVTVPLGFLKEHQDTFFEPPLPAKKAEAIKKLGFGTNNKIFLEFEEPFWEPDCQFIQV
                     VWEDTSPLQDTALSLQDTWFKKLIGFLVQPSFESSHVLCGFIAGLESEFMETLSDEEV
                     LLSLTQVLRRVTGNPQLPAAKSVRRSQWHSAPYTRGSYSYVAVGSTGDDLDLMAQPLP
                     EDGTGTQLQVLFAGEATHRTFYSTTHGALLSGWREADRLVSLWDSQVEQSRPRL"
     exon            199..688
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /inference="alignment:Splign:2.1.0"
     exon            689..888
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1141
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1254
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1412
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1737
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggcgcctaa ggccaggtct ctggaggacc ctgagctagc catggcgttc cctggcccgc
       61 gggtgctggt ggtgggcagc ggcatcgcgg ggctgggcgc tgcgcagaag ctctgcagcc
      121 accgggctgc tccacacctg cgggttctgg aggccaccgc cagcgccggg ggccgcatcc
      181 gctcggaacg ctgcttcggt ggtgtagtgg agctgggtgc acactggatc catgggccct
      241 cccaggacaa tcctgtcttc cagctggctg cagaattcgg gctcctgggg gagaaagaat
      301 tatcggaaga gaaccagctt gtggatacag gagggcacgt ggccctgccc tccatgatct
      361 ggtccagctc tggaacaagt gtgagcctgg agctgatgac agagatggcc cgtctgttct
      421 atggactgat agagcggacc cgagaattcc tgaatgaatc tgaaacccca atggccagtg
      481 ttggagagtt cctgaagaag gagataagtc agcaggtggc tagctggacc gaggatgatg
      541 aggacaccag aaagcgcaag ctggctatcc tgaatacttt cttcaacata gagtgttgtg
      601 tgagcggcac ccacagcatg gacctggtgg ctcttgcacc gttcggggag tatacagtgc
      661 tgcctgggct ggattgcatc ttggctggtg gctaccaagg acttaccgac cgcatactgg
      721 cttccttgcc caaggatacg gtcgcttttg acaagccggt gaagaccatt cactggaatg
      781 ggtccttcca ggaagctgct tttccagggg agaccttccc cgtattggtg gagtgtgaag
      841 atggtgcccg cctgcctgca catcatgtca ttgtcacagt gccattaggt tttcttaaag
      901 aacaccaaga tacctttttt gagccaccac tgcctgccaa aaaagcagaa gccatcaaaa
      961 aactaggctt tggtaccaac aacaaaatct tcttggagtt tgaagagccc ttctgggagc
     1021 ccgactgcca gttcatccag gtggtgtggg aagatacatc gcccttacag gacacagccc
     1081 tctcactcca agacacctgg ttcaagaagc tcattggatt tttggtccag ccttcttttg
     1141 agtcttcaca tgtgctctgt gggtttattg ctgggctgga gtcggagttt atggagactc
     1201 tgtcagatga agaagtgctt ctgtctctaa cccaagtact ccggagagtg acaggaaacc
     1261 cacaattgcc agcagccaag agcgtacgaa ggtctcagtg gcacagtgcc ccgtacaccc
     1321 gaggttccta cagctatgtg gctgtgggca gcactggcga tgacctagat ctgatggctc
     1381 agcccctccc tgaagatgga actggcaccc agctccaggt actctttgct ggggaagcca
     1441 cacatcggac attttattct accacacatg gggccctcct gtctggctgg agagaagctg
     1501 atcgcctcgt tagtctgtgg gactcacagg tggagcagtc gcggcccagg ctgtgagtgc
     1561 ctgctgctct gctccaccac ttgggctgac caagctctgt ttggtttgag gtttggggat
     1621 cttactggta gctttaattt tggggaagtg gttttgtttt tcttatctgt tggagtctgg
     1681 cttggtctga tcatggatgc cccgttaagt gttgtacgca taaagcagtt aacattg
//
