LOCUS       NP_722478                504 aa            linear   ROD 30-APR-2024
DEFINITION  peroxisomal N(1)-acetyl-spermine/spermidine oxidase isoform 1 [Mus
            musculus].
ACCESSION   NP_722478
VERSION     NP_722478.2
DBSOURCE    REFSEQ: accession NM_153783.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 504)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 504)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (residues 1 to 504)
  AUTHORS   Tormos,J.R., Henderson Pozzi,M. and Fitzpatrick,P.F.
  TITLE     Mechanistic studies of the role of a conserved histidine in a
            mammalian polyamine oxidase
  JOURNAL   Arch Biochem Biophys 528 (1), 45-49 (2012)
   PUBMED   22959971
  REMARK    GeneRIF: Steady-state kinetics of His64 mutant proteins shows that
            the mutations result in a decrease of ~15-fold in the rate constant
            for amine oxidation.
REFERENCE   4  (residues 1 to 504)
  AUTHORS   Henderson Pozzi,M. and Fitzpatrick,P.F.
  TITLE     A lysine conserved in the monoamine oxidase family is involved in
            oxidation of the reduced flavin in mouse polyamine oxidase
  JOURNAL   Arch Biochem Biophys 498 (2), 83-88 (2010)
   PUBMED   20417173
REFERENCE   5  (residues 1 to 504)
  AUTHORS   Pozzi,M.H., Gawandi,V. and Fitzpatrick,P.F.
  TITLE     Mechanistic studies of para-substituted
            N,N'-dibenzyl-1,4-diaminobutanes as substrates for a mammalian
            polyamine oxidase
  JOURNAL   Biochemistry 48 (51), 12305-12313 (2009)
   PUBMED   19911805
  REMARK    GeneRIF: N,N'-dibenzyl-1,4-diaminobutanes, with only two nitrogens,
            are also substrates of polyamine oxidase.
REFERENCE   6  (residues 1 to 504)
  AUTHORS   Bianchi,M., Polticelli,F., Ascenzi,P., Botta,M., Federico,R.,
            Mariottini,P. and Cona,A.
  TITLE     Inhibition of polyamine and spermine oxidases by polyamine
            analogues
  JOURNAL   FEBS J 273 (6), 1115-1123 (2006)
   PUBMED   16519678
REFERENCE   7  (residues 1 to 504)
  AUTHORS   Royo,M. and Fitzpatrick,P.F.
  TITLE     Mechanistic studies of mouse polyamine oxidase with
            N1,N12-bisethylspermine as a substrate
  JOURNAL   Biochemistry 44 (18), 7079-7084 (2005)
   PUBMED   15865452
REFERENCE   8  (residues 1 to 504)
  AUTHORS   Chaturvedi,R., Cheng,Y., Asim,M., Bussiere,F.I., Xu,H.,
            Gobert,A.P., Hacker,A., Casero,R.A. Jr. and Wilson,K.T.
  TITLE     Induction of polyamine oxidase 1 by Helicobacter pylori causes
            macrophage apoptosis by hydrogen peroxide release and mitochondrial
            membrane depolarization
  JOURNAL   J Biol Chem 279 (38), 40161-40173 (2004)
   PUBMED   15247269
  REMARK    GeneRIF: new mechanism for pathogen-induced oxidative stress in
            macrophages in which activation of PAO1 leads to H(2)O(2) release
            and apoptosis by a mitochondrial-dependent cell death pathway
REFERENCE   9  (residues 1 to 504)
  AUTHORS   Wu,T., Yankovskaya,V. and McIntire,W.S.
  TITLE     Cloning, sequencing, and heterologous expression of the murine
            peroxisomal flavoprotein, N1-acetylated polyamine oxidase
  JOURNAL   J Biol Chem 278 (23), 20514-20525 (2003)
   PUBMED   12660232
  REMARK    GeneRIF: translated amino acid sequences of mpao and bpao have a
            -Pro-Arg-Leu peroxisomal targeting signal at the extreme C termini
            and a beta-alpha-beta FAD-binding motif is present in the
            N-terminal portion of mPAO.
REFERENCE   10 (residues 1 to 504)
  AUTHORS   Vujcic,S., Liang,P., Diegelman,P., Kramer,D.L. and Porter,C.W.
  TITLE     Genomic identification and biochemical characterization of the
            mammalian polyamine oxidase involved in polyamine back-conversion
  JOURNAL   Biochem J 370 (Pt 1), 19-28 (2003)
   PUBMED   12477380
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC109205.12.
            
            On Jan 31, 2003 this sequence version replaced NP_722478.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC082783.1, AK030664.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 85.14 cM"
     Protein         1..504
                     /product="peroxisomal N(1)-acetyl-spermine/spermidine
                     oxidase isoform 1"
                     /EC_number="1.5.3.13"
                     /note="peroxisomal N1-acetyl-spermine/spermidine oxidase;
                     peroxisomal N(1)-acetyl-spermine/spermidine oxidase;
                     exo-N4-amino"
                     /calculated_mol_wt=55316
     Region          6..490
                     /region_name="PLN02568"
                     /note="polyamine oxidase"
                     /db_xref="CDD:215308"
     Region          502..504
                     /region_name="Microbody targeting signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8C0L6.3)"
     CDS             1..504
                     /gene="Paox"
                     /gene_synonym="2410012F02Rik; Pao"
                     /coded_by="NM_153783.4:42..1556"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS21965.1"
                     /db_xref="GeneID:212503"
                     /db_xref="MGI:MGI:1916983"
ORIGIN      
        1 mafpgprvlv vgsgiaglga aqklcshraa phlrvleata saggrirser cfggvvelga
       61 hwihgpsqdn pvfqlaaefg llgekelsee nqlvdtgghv alpsmiwsss gtsvslelmt
      121 emarlfygli ertreflnes etpmasvgef lkkeisqqva swteddedtr krklailntf
      181 fnieccvsgt hsmdlvalap fgeytvlpgl dcilaggyqg ltdrilaslp kdtvafdkpv
      241 ktihwngsfq eaafpgetfp vlvecedgar lpahhvivtv plgflkehqd tffepplpak
      301 kaeaikklgf gtnnkiflef eepfwepdcq fiqvvwedts plqdtalslq dtwfkkligf
      361 lvqpsfessh vlcgfiagle sefmetlsde evllsltqvl rrvtgnpqlp aaksvrrsqw
      421 hsapytrgsy syvavgstgd dldlmaqplp edgtgtqlqv lfageathrt fystthgall
      481 sgwreadrlv slwdsqveqs rprl
//
