LOCUS       NM_001357127            4120 bp    mRNA    linear   ROD 24-DEC-2022
DEFINITION  Mus musculus peptidylglycine alpha-amidating monooxygenase (Pam),
            transcript variant 2, mRNA.
ACCESSION   NM_001357127 XM_006529244
VERSION     NM_001357127.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Powers KG, Ma XM, Eipper BA and Mains RE.
  TITLE     Cell-type specific knockout of peptidylglycine alpha-amidating
            monooxygenase reveals specific behavioral roles in excitatory
            forebrain neurons and cardiomyocytes
  JOURNAL   Genes Brain Behav 20 (2), e12699 (2021)
   PUBMED   32902163
  REMARK    GeneRIF: Cell-type specific knockout of peptidylglycine
            alpha-amidating monooxygenase reveals specific behavioral roles in
            excitatory forebrain neurons and cardiomyocytes.
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Back N, Luxmi R, Powers KG, Mains RE and Eipper BA.
  TITLE     Peptidylglycine alpha-amidating monooxygenase is required for
            atrial secretory granule formation
  JOURNAL   Proc Natl Acad Sci U S A 117 (30), 17820-17831 (2020)
   PUBMED   32661174
REFERENCE   3  (bases 1 to 4120)
  AUTHORS   Chen YC, Mains RE, Eipper BA, Hoffman BG, Czyzyk TA, Pintar JE and
            Verchere CB.
  TITLE     PAM haploinsufficiency does not accelerate the development of diet-
            and human IAPP-induced diabetes in mice
  JOURNAL   Diabetologia 63 (3), 561-576 (2020)
   PUBMED   31984442
  REMARK    Erratum:[Diabetologia. 2020 May;63(5):1090. PMID: 32172312]
REFERENCE   4  (bases 1 to 4120)
  AUTHORS   Powers KG, Ma XM, Eipper BA and Mains RE.
  TITLE     Identifying roles for peptidergic signaling in mice
  JOURNAL   Proc Natl Acad Sci U S A 116 (40), 20169-20179 (2019)
   PUBMED   31455734
REFERENCE   5  (bases 1 to 4120)
  AUTHORS   Quarta C, Fisette A, Xu Y, Collden G, Legutko B, Tseng YT, Reim A,
            Wierer M, De Rosa MC, Klaus V, Rausch R, Thaker VV, Graf E, Strom
            TM, Poher AL, Gruber T, Le Thuc O, Cebrian-Serrano A, Kabra D,
            Bellocchio L, Woods SC, Pflugfelder GO, Nogueiras R, Zeltser L,
            Grunwald Kadow IC, Moon A, Garcia-Caceres C, Mann M, Treier M,
            Doege CA and Tschop MH.
  TITLE     Functional identity of hypothalamic melanocortin neurons depends on
            Tbx3
  JOURNAL   Nat Metab 1 (2), 222-235 (2019)
   PUBMED   32694784
REFERENCE   6  (bases 1 to 4120)
  AUTHORS   Alam MR, Caldwell BD, Johnson RC, Darlington DN, Mains RE and
            Eipper BA.
  TITLE     Novel proteins that interact with the COOH-terminal cytosolic
            routing determinants of an integral membrane peptide-processing
            enzyme
  JOURNAL   J Biol Chem 271 (45), 28636-28640 (1996)
   PUBMED   8910496
REFERENCE   7  (bases 1 to 4120)
  AUTHORS   Shirozu M, Tada H, Tashiro K, Nakamura T, Lopez ND, Nazarea M,
            Hamada T, Sato T, Nakano T and Honjo T.
  TITLE     Characterization of novel secreted and membrane proteins isolated
            by the signal sequence trap method
  JOURNAL   Genomics 37 (3), 273-280 (1996)
   PUBMED   8938438
REFERENCE   8  (bases 1 to 4120)
  AUTHORS   Burmeister M, Ren Q, Makris GJ, Samson D and Bennett V.
  TITLE     Genes for the neuronal immunoglobulin domain cell adhesion
            molecules neurofascin and Nr-CAM map to mouse chromosomes 1 and 12
            and homologous human chromosomes
  JOURNAL   Mamm Genome 7 (7), 558-559 (1996)
   PUBMED   8672144
REFERENCE   9  (bases 1 to 4120)
  AUTHORS   Lossie AC, Eipper BA, Hand TA and Camper SA.
  TITLE     Localization of the peptidylglycine alpha-amidating monooxygenase
            gene (Pam) introduces a region of homology between human chromosome
            5q and mouse chromosome 1
  JOURNAL   Mamm Genome 5 (11), 738-739 (1994)
   PUBMED   7873888
REFERENCE   10 (bases 1 to 4120)
  AUTHORS   Ouafik LH, Stoffers DA, Campbell TA, Johnson RC, Bloomquist BT,
            Mains RE and Eipper BA.
  TITLE     The multifunctional peptidylglycine alpha-amidating monooxygenase
            gene: exon/intron organization of catalytic, processing, and
            routing domains
  JOURNAL   Mol Endocrinol 6 (10), 1571-1584 (1992)
   PUBMED   1448112
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157923.4 and AC102191.11.
            
            [WARNING] On May 10, 2023 this sequence was replaced by
            NM_001357127.2.
            
            On Oct 21, 2017 this sequence version replaced XM_006529244.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.233262.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AC157923.4         10925-11086
            163-565             AC157923.4         129312-129714
            566-686             AC157923.4         130666-130786
            687-744             AC157923.4         161894-161951
            745-832             AC157923.4         171833-171920
            833-918             AC157923.4         182055-182140
            919-1002            AC157923.4         183394-183477
            1003-1051           AC102191.11        17112-17160
            1052-1119           AC102191.11        18226-18293
            1120-1200           AC102191.11        19452-19532
            1201-1277           AC102191.11        19793-19869
            1278-1381           AC102191.11        21037-21140
            1382-1566           AC102191.11        29715-29899
            1567-1638           AC102191.11        31304-31375
            1639-1956           AC102191.11        50981-51298
            1957-2086           AC102191.11        62269-62398
            2087-2203           AC102191.11        70789-70905
            2204-2276           AC102191.11        73197-73269
            2277-2487           AC102191.11        74592-74802
            2488-2688           AC102191.11        75021-75221
            2689-2804           AC102191.11        77423-77538
            2805-2913           AC102191.11        80985-81093
            2914-2967           AC102191.11        83993-84046
            2968-3171           AC102191.11        89461-89664
            3172-3225           AC102191.11        92512-92565
            3226-4120           AC102191.11        93513-94407
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 47.76 cM"
     gene            1..4120
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /note="peptidylglycine alpha-amidating monooxygenase"
                     /db_xref="GeneID:18484"
                     /db_xref="MGI:MGI:97475"
     exon            1..162
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            163..565
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     CDS             465..3404
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /EC_number="1.14.17.3"
                     /EC_number="4.3.2.5"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; PEPTIDYL-GLYCINE ALPHA-AMIDATING MONOOXYGENASE
                     PRECURSOR (PAM)"
                     /codon_start=1
                     /product="peptidyl-glycine alpha-amidating monooxygenase
                     isoform 2 precursor"
                     /protein_id="NP_001344056.1"
                     /db_xref="CCDS:CCDS87863.1"
                     /db_xref="GeneID:18484"
                     /db_xref="MGI:MGI:97475"
                     /translation="MAGRARSRLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFSN
                     ECLGTTRPITPIDSSDFTLDIRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRASM
                     DTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGE
                     TGSKYFVLQVHYGDISAFRDNHKDCSGVSLHLTRVPQPLIAGMYLMMSVNTVIPPGEK
                     VVNSDISCHYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPV
                     EHPVDVAFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCT
                     QNVAPDMFRTIPEEANIPIPVKSDMVMIHGHHKETENKEKSALIQQPKQGEEEAFEQG
                     DFYSLLSKLLGEREDVVHVHKYNPTEKTESGSDLVAEIANVVQKKDLGRSDAREGAEH
                     EEGGNAILVRDRIHKFHRLESTLRPAESRALSFQQPGEGPWEPELAGDFHVEEALEWP
                     GVYLLPGQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEEDTILVID
                     PNKAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLEPRSKEGPLLVLGRSM
                     QPGSDQNHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFSPSGKFITQWGEESSGSSPK
                     PGQFSVPHSLALVPHLNQLCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYI
                     PGFLFAVNGKPYFGDQEPVQGFVMNFSSGEIIDVFKPVRKHFDMPHDIVASEDGTVYI
                     GDAHTNTVWKFTLTESRLEVEHRSVKKAGIEVPEIKEAEAVVEPKVKNKPTSSELQKM
                     QEKKKLIKDPGSGVPVVLITTLLVIPVVVLLAIAMFIRWKKSRAFGDHDRKLESSSGR
                     VLGRLRGKGSSGLNLGNFFASRKGYSRKGFDRVSTEGSDQEKDEDDGSESEEEYSAPL
                     PTPAPSS"
     exon            566..686
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            687..744
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            745..832
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            833..918
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1002
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1051
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1119
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1200
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1277
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1381
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1566
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1638
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1956
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2086
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2203
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2204..2276
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2487
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2688
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2804
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2913
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2914..2967
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            2968..3171
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3225
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
     exon            3226..4120
                     /gene="Pam"
                     /gene_synonym="PHM"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggtcccggag cccagcgcgc ggcccgggaa ggaaggaggc cagcagcggc cgcggccgcg
       61 tgctcagcct gtccccgccc tgggacccgg agcctccaac taactccgcc cgccgcccgc
      121 gggctcccgc ttccgtcccg ggaagagcct gcggccgcgc aggttccgaa tgatgactga
      181 cgcgggtctg cgcgatagcc ctcacagccc ctggcgttgt gagcgataag gaccctgctc
      241 atccagccct gtggctgcac cggctcccgg acgctctgga gggcgggcgg ctcccgcggg
      301 cgggcggagc ggactccagc gccgcttccc acgcgtccgg agcggacgga gcggacctgg
      361 ctggccgcgc atcgccctcg cctggggcca tgaagtagct gcggccgcca gccttgccct
      421 cccgctggcc cggccactgc ccgctgcccc ggtcctgcgc ggacatggcc ggacgcgccc
      481 gcagccgcct gctgctgctg ctggggctgc tcgccttgca gagcagctgc ctggccttca
      541 gaagcccact ttctgtcttt aagaggttta aagaaactac cagatcattt tccaatgaat
      601 gccttggtac caccagaccc atcactccta ttgattcttc ggattttaca ctggatattc
      661 gcatgcctgg ggtcacacct aaagagtctg acacgtactt ctgcatgtcc atgcgtctgc
      721 ctgtggatga ggaagccttc gtgattgact tcaagcctcg ggccagcatg gacactgtcc
      781 accatatgct gctgtttgga tgcaacatgc cctcatccac tgggagttac tggttttgtg
      841 atgaaggaac ctgtacagat aaagccaata ttctatatgc ctgggcaaga aatgctcccc
      901 ccacccggct ccctaaaggt gttggattca gagttggagg ggaaactgga agcaaatact
      961 tcgtccttca agttcactat ggggacatca gtgcttttcg agataatcac aaagactgtt
     1021 ccggtgtgtc cttacatctc acacgtgtgc cacagccttt gattgcgggc atgtacctta
     1081 tgatgtctgt caacactgtt atacccccag gagagaaagt agtgaattct gacatttcgt
     1141 gccattacaa aatgtatcca atgcatgtgt ttgcctaccg agtccatact caccatttag
     1201 gtaaggtagt gagtggatac agagtaagaa acggacagtg gacgctgatt ggacgccaga
     1261 gcccccagct gccacaggct ttttacccag tggaacaccc agtagatgtt gcttttggtg
     1321 atatactggc agccagatgt gtgttcactg gtgaggggag gaccgaagcc acccacatcg
     1381 gcggcacatc tagtgatgaa atgtgcaact tgtacattat gtattacatg gaagccaagc
     1441 atgcagtttc cttcatgacc tgtacacaga atgtggctcc agatatgttc agaactatcc
     1501 cagaagaggc caatattcca attcctgtaa aatccgacat ggttatgatc catgggcatc
     1561 acaaagaaac agaaaacaaa gaaaagagtg ctttaataca gcagccaaag cagggagagg
     1621 aggaagcctt cgagcagggt gatttctatt cactactttc caagctgcta ggagaaaggg
     1681 aagatgttgt tcatgtgcac aaatataatc ctacagaaaa gacagaatct gggtcagacc
     1741 tggtagctga gattgcaaac gtagtccaga aaaaggacct tggtcggtct gacgccagag
     1801 agggagcgga gcatgaggaa gggggtaatg ctatcctggt cagagacagg atccacaaat
     1861 tccacaggct agagtcaacc ttgaggccag ctgagagcag agctctatcc ttccagcagc
     1921 ctggtgaagg tccttgggaa ccagaactcg caggagattt ccatgtggaa gaagcactgg
     1981 agtggcctgg agtgtacttg ttaccaggcc aggtttctgg ggtggccctg gattctaaga
     2041 ataaccttgt gattttccac agaggtgacc atgtttggga tggaaactct tttgacagca
     2101 agttcgttta ccaacaaaga ggtcttggac caattgaaga agataccatc ctggtcattg
     2161 acccaaataa agctgaaata ctccagtcca gcggcaagaa tctgttttat ttaccgcatg
     2221 gtttgagcat agatacagat ggaaattatt gggtcacgga tgtggctctc catcaggtgt
     2281 tcaaattgga accacgtagc aaagaaggcc ctctcttagt tctgggaagg agcatgcaac
     2341 ctggcagcga ccaaaatcat ttctgccagc ccactgatgt ggcggtggag cccagtactg
     2401 gagctgtctt cgtgtcagat ggttactgca acagtcggat cgtgcagttc tcaccaagcg
     2461 ggaagttcat cactcagtgg ggagaagagt cttctgggag cagtcctaag ccagggcagt
     2521 tcagtgttcc tcacagtttg gcccttgtgc ctcacctgaa ccagttgtgt gtggccgata
     2581 gggaaaatgg ccggatccag tgtttcaaga ctgacaccaa agaatttgtg agagagatta
     2641 agcatgcgtc atttggaagg aatgtctttg caatctcata tataccaggt ttcctctttg
     2701 cagtaaatgg gaagccttac tttggagacc aagaacccgt gcaaggattt gtgatgaact
     2761 tttccagtgg ggaaattata gatgttttca aaccagttcg caagcacttt gacatgcctc
     2821 atgatattgt ggcttctgaa gatgggactg tgtacattgg cgacgcacac acaaacaccg
     2881 tgtggaagtt caccctcact gaaagcaggc tggaagtgga gcatcgatca gttaaaaagg
     2941 ctggcattga ggtcccggaa atcaaagaag ccgaggcagt tgttgaaccc aaagtgaaga
     3001 acaaacccac ctcctcagaa ttgcagaaga tgcaggagaa aaagaaactg atcaaagatc
     3061 caggctcggg agtgcctgtg gttctcatta caacccttct ggttatccct gtggttgtcc
     3121 tgctggccat tgccatgttt attcgatgga aaaaatcaag ggcctttgga gatcatgacc
     3181 gaaagctcga gtcgagttct ggaagagtac tgggaagact ccgaggaaag ggaagcagcg
     3241 gcttaaatct aggaaatttc tttgcaagcc ggaaaggcta cagcagaaaa gggttcgacc
     3301 gagtgagcac agaggggagt gaccaagaga aggacgaaga tgatggaagt gagtctgaag
     3361 aggagtactc ggctccgctg cccactcctg caccttcctc ctgaactcca gccttccttc
     3421 cgtggagctc gagtgaggtt tacggaggtg cccagattcc tttcccttta acgcgtgtaa
     3481 agttctgtgc atttgattgt aaactgtact cgtccgtgtg gaactgtaca caccttagtt
     3541 acttcatttg gctccgttgg cttgtgtttc taggtgagga gtttcctacc agttcactcc
     3601 agtgccattg tctttatatg aacgtagtgt agagaagctg ccctcctctt ccatggtagc
     3661 gctcaaagcc ctaatggaag tttagctcat ttacatttgg agaccctttt ggttttttgg
     3721 ttttggtttt ggtttggttt tttttttttt tttttttttt ttttttttgt ggatgtaaat
     3781 agccctattc tctgcttgaa cacagtattc tcccagtcca cacccattgc cagtgtcttt
     3841 ctttggtgcc tttcctgttc agcattctca gcctgtggca gtaaagagaa actttgctac
     3901 acaacaaaaa gctgctaaat ctccctctat ttttttaaaa tcactaacat tatattgcaa
     3961 tgagagaaat tttaaaaagt ctctatttaa attctttttt taaatttctc ttcagttggt
     4021 gtgtttccgg gatgtcttat ttttagatgg ttacactgtt agaacactat ttttcagaat
     4081 ctgaatgtaa tttgtgtaat aaagtgtttt cagagcatta
//
