LOCUS       NP_062525                675 aa            linear   ROD 27-APR-2025
DEFINITION  protein PALS1 [Mus musculus].
ACCESSION   NP_062525
VERSION     NP_062525.1
DBSOURCE    REFSEQ: accession NM_019579.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 675)
  AUTHORS   Groh,A.C., Moller-Kerutt,A., Gilhaus,K., Hoffken,V., Nedvetsky,P.,
            Kleimann,S., Behrens,M., Ghosh,S., Hansen,U., Krahn,M.P., Ebnet,K.,
            Pavenstadt,H., Ludwig,A. and Weide,T.
  TITLE     PALS1 is a key regulator of the lateral distribution of tight
            junction proteins in renal epithelial cells
  JOURNAL   J Cell Sci 137 (5) (2024)
   PUBMED   38265145
  REMARK    GeneRIF: PALS1 is a key regulator of the lateral distribution of
            tight junction proteins in renal epithelial cells.
REFERENCE   2  (residues 1 to 675)
  AUTHORS   Gredler,M.L. and Zallen,J.A.
  TITLE     Multicellular rosettes link mesenchymal-epithelial transition to
            radial intercalation in the mouse axial mesoderm
  JOURNAL   Dev Cell 58 (11), 933-950 (2023)
   PUBMED   37080203
REFERENCE   3  (residues 1 to 675)
  AUTHORS   Sterling,N.A., Park,J.Y., Park,R., Cho,S.H. and Kim,S.
  TITLE     An entosis-like process induces mitotic disruption in Pals1
            microcephaly pathogenesis
  JOURNAL   Nat Commun 14 (1), 82 (2023)
   PUBMED   36604424
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 675)
  AUTHORS   Brault,J.B., Bardin,S., Lampic,M., Carpentieri,J.A., Coquand,L.,
            Penisson,M., Lachuer,H., Victoria,G.S., Baloul,S., El Marjou,F.,
            Boncompain,G., Miserey-Lenkei,S., Belvindrah,R., Fraisier,V.,
            Francis,F., Perez,F., Goud,B. and Baffet,A.D.
  TITLE     RAB6 and dynein drive post-Golgi apical transport to prevent
            neuronal progenitor delamination
  JOURNAL   EMBO Rep 23 (10), e54605 (2022)
   PUBMED   35979738
REFERENCE   5  (residues 1 to 675)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 675)
  AUTHORS   Wang,Q., Hurd,T.W. and Margolis,B.
  TITLE     Tight junction protein Par6 interacts with an evolutionarily
            conserved region in the amino terminus of PALS1/stardust
  JOURNAL   J Biol Chem 279 (29), 30715-30721 (2004)
   PUBMED   15140881
REFERENCE   7  (residues 1 to 675)
  AUTHORS   Hurd,T.W., Gao,L., Roh,M.H., Macara,I.G. and Margolis,B.
  TITLE     Direct interaction of two polarity complexes implicated in
            epithelial tight junction assembly
  JOURNAL   Nat Cell Biol 5 (2), 137-142 (2003)
   PUBMED   12545177
REFERENCE   8  (residues 1 to 675)
  AUTHORS   Makarova,O., Roh,M.H., Liu,C.J., Laurinec,S. and Margolis,B.
  TITLE     Mammalian Crumbs3 is a small transmembrane protein linked to
            protein associated with Lin-7 (Pals1)
  JOURNAL   Gene 302 (1-2), 21-29 (2003)
   PUBMED   12527193
REFERENCE   9  (residues 1 to 675)
  AUTHORS   Roh,M.H., Makarova,O., Liu,C.J., Shin,K., Lee,S., Laurinec,S.,
            Goyal,M., Wiggins,R. and Margolis,B.
  TITLE     The Maguk protein, Pals1, functions as an adapter, linking
            mammalian homologues of Crumbs and Discs Lost
  JOURNAL   J Cell Biol 157 (1), 161-172 (2002)
   PUBMED   11927608
REFERENCE   10 (residues 1 to 675)
  AUTHORS   Kamberov,E., Makarova,O., Roh,M., Liu,A., Karnak,D., Straight,S.
            and Margolis,B.
  TITLE     Molecular cloning and characterization of Pals, proteins associated
            with mLin-7
  JOURNAL   J Biol Chem 275 (15), 11425-11431 (2000)
   PUBMED   10753959
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB858385.1, AK032731.1, BC138624.1, AK133187.1, AK040265.1 and
            AW488563.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC075698.1, SRR1660813.180875.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 35.51 cM"
     Protein         1..675
                     /product="protein PALS1"
                     /note="MAGUK p55 subfamily member 5; protein associated
                     with Lin-7 1; membrane protein, palmitoylated 3 (MAGUK p55
                     subfamily member 5); membrane protein, palmitoylated 5
                     (MAGUK p55 subfamily member 5)"
                     /calculated_mol_wt=77099
     Region          1..345
                     /region_name="Required for the correct localization of
                     PALS1 and PATJ at cell-cell contacts and the normal
                     formation of tight junctions and adherens junctions.
                     /evidence=ECO:0000269|PubMed:17182851"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Site            14
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8N3R9; propagated from
                     UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Region          21..140
                     /region_name="Interaction with PARD6B.
                     /evidence=ECO:0000269|PubMed:15140881"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Site            25
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Region          51..79
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Site            83
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8N3R9; propagated from
                     UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Site            84
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8N3R9; propagated from
                     UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Region          123..170
                     /region_name="L27_N"
                     /note="pfam09060"
                     /db_xref="CDD:462666"
     Region          181..243
                     /region_name="Interaction with LIN7C.
                     /evidence=ECO:0000269|PubMed:10753959"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JLB2.1)"
     Region          189..237
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          255..334
                     /region_name="PDZ_MPP5-like"
                     /note="PDZ domain of membrane palmitoylated protein 5
                     (MPP5), Drosophila Stardust, and related domains; cd06798"
                     /db_xref="CDD:467259"
     Site            order(266..272,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467259"
     Region          349..411
                     /region_name="SH3_MPP5"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated 5 (or MAGUK p55 subfamily member 5);
                     cd12036"
                     /db_xref="CDD:212969"
     Site            order(351,375..376)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212969"
     Site            order(354,356,359,370,388..389,408,410..411)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212969"
     Site            order(486,492,509,516,519,528,551,556)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(486,492)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     Region          491..661
                     /region_name="GuKc"
                     /note="Guanylate kinase homologues; smart00072"
                     /db_xref="CDD:214504"
     CDS             1..675
                     /gene="Pals1"
                     /gene_synonym="3830420B02Rik; Mpp5"
                     /coded_by="NM_019579.3:408..2435"
                     /db_xref="CCDS:CCDS26001.1"
                     /db_xref="GeneID:56217"
                     /db_xref="MGI:MGI:1927339"
ORIGIN      
        1 mttsymnghv teesdsgikn ldlaspeeyp khremavdcp gdlgtrmmpv rrsaqlerir
       61 qqqedmrrrr eeegkkqeld lnssmrlkkl aqippktgid npifdteegi vlesphyavn
      121 ildvedlfss lkhiqhtlvd sqsqedisll lqlvqnrdfq nafkihnavt vhmskasppf
      181 plianvqdlv qevqtvlkpv hqkegqelta llnaphiqal llahdkvaeq emqlepitde
      241 rvyesighyg getvkivrie kardiplgat vrnemdsvii srivkggaae ksgllhegde
      301 vleingieir gkdvnevfdl lsdmhgtltf vlipsqqikp ppaketvihv kahfdydpsd
      361 dpyvpcrelg lsfqkgdilh visqedpnww qayregdedn qplaglvpgk sfqqqreamk
      421 qtieedkepe ksgklwcakk nkkkrkkvly nanknddydn eeiltyeems lyhqpanrkr
      481 piiligpqnc gqnelrqrlm nkekdrfasa vphttrnrrd hevagrdyhf vsrqafeadi
      541 aagkfiehge feknlygtsi dsvrqvinsg kicllslrtq slktlrnsdl kpyiifiapp
      601 sqerlralla kegknpkpee lreiiektre meqnnghyfd taivnsdldk ayqellrlin
      661 kldtepqwvp stwlr
//
