LOCUS       NM_027470               2898 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus p21 (RAC1) activated kinase 4 (Pak4), mRNA.
ACCESSION   NM_027470
VERSION     NM_027470.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Wu,D., Yu,H.C., Cha,H.N., Park,S., Lee,Y., Yoon,S.J., Park,S.Y.,
            Park,B.H. and Bae,E.J.
  TITLE     PAK4 phosphorylates and inhibits AMPKalpha to control glucose
            uptake
  JOURNAL   Nat Commun 15 (1), 6858 (2024)
   PUBMED   39127697
  REMARK    GeneRIF: PAK4 phosphorylates and inhibits AMPKalpha to control
            glucose uptake.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Ma,Y., Dumesny,C., Dong,L., Ang,C.S., Asadi,K., Zhan,Y.,
            Nikfarjam,M. and He,H.
  TITLE     Inhibition of P21-activated kinases 1 and 4 synergistically
            suppresses the growth of pancreatic cancer by stimulating
            anti-tumour immunity
  JOURNAL   Cell Commun Signal 22 (1), 287 (2024)
   PUBMED   38797819
  REMARK    GeneRIF: Inhibition of P21-activated kinases 1 and 4
            synergistically suppresses the growth of pancreatic cancer by
            stimulating anti-tumour immunity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2898)
  AUTHORS   Hu,B., Moiseev,D., Schena,I., Faezov,B., Dunbrack,R., Chernoff,J.
            and Li,J.
  TITLE     PAK2 is necessary for myelination in the peripheral nervous system
  JOURNAL   Brain 147 (5), 1809-1821 (2024)
   PUBMED   38079473
REFERENCE   4  (bases 1 to 2898)
  AUTHORS   Yu,H.C., Jeon,Y.G., Na,A.Y., Han,C.Y., Lee,M.R., Yang,J.D.,
            Yu,H.C., Son,J.B., Kim,N.D., Kim,J.B., Lee,S., Bae,E.J. and
            Park,B.H.
  TITLE     p21-activated kinase 4 counteracts PKA-dependent lipolysis by
            phosphorylating FABP4 and HSL
  JOURNAL   Nat Metab 6 (1), 94-112 (2024)
   PUBMED   38216738
  REMARK    GeneRIF: p21-activated kinase 4 counteracts PKA-dependent lipolysis
            by phosphorylating FABP4 and HSL.
REFERENCE   5  (bases 1 to 2898)
  AUTHORS   Shi,M.Y., Yu,H.C., Han,C.Y., Bang,I.H., Park,H.S., Jang,K.Y.,
            Lee,S., Son,J.B., Kim,N.D., Park,B.H. and Bae,E.J.
  TITLE     p21-activated kinase 4 suppresses fatty acid beta-oxidation and
            ketogenesis by phosphorylating NCoR1
  JOURNAL   Nat Commun 14 (1), 4987 (2023)
   PUBMED   37591884
  REMARK    GeneRIF: p21-activated kinase 4 suppresses fatty acid
            beta-oxidation and ketogenesis by phosphorylating NCoR1.
            Erratum:[Nat Commun. 2023 Nov 2;14(1):7007. doi:
            10.1038/s41467-023-42957-1.. PMID: 37919334]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2898)
  AUTHORS   Li,X. and Minden,A.
  TITLE     Targeted disruption of the gene for the PAK5 kinase in mice
  JOURNAL   Mol Cell Biol 23 (20), 7134-7142 (2003)
   PUBMED   14517284
  REMARK    Erratum:[Mol Cell Biol. 2004 Jun;24(12):5637]
REFERENCE   7  (bases 1 to 2898)
  AUTHORS   Qu,J., Li,X., Novitch,B.G., Zheng,Y., Kohn,M., Xie,J.M., Kozinn,S.,
            Bronson,R., Beg,A.A. and Minden,A.
  TITLE     PAK4 kinase is essential for embryonic viability and for proper
            neuronal development
  JOURNAL   Mol Cell Biol 23 (20), 7122-7133 (2003)
   PUBMED   14517283
  REMARK    GeneRIF: PAK4 has a role in neural tube development
REFERENCE   8  (bases 1 to 2898)
  AUTHORS   Lu,Y., Pan,Z.Z., Devaux,Y. and Ray,P.
  TITLE     p21-activated protein kinase 4 (PAK4) interacts with the
            keratinocyte growth factor receptor and participates in
            keratinocyte growth factor-mediated inhibition of oxidant-induced
            cell death
  JOURNAL   J Biol Chem 278 (12), 10374-10380 (2003)
   PUBMED   12529371
  REMARK    GeneRIF: PAK4 interacts with KGF receptor and mediate
            anti-apoptosis effects of KGF on epithelial cells.
REFERENCE   9  (bases 1 to 2898)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2898)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY197606.1, BB839524.1, CF172418.1, BQ572351.1, AC136456.3 and
            AK017713.1.
            
            On Dec 20, 2006 this sequence version replaced NM_027470.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK129298.2, AK088512.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-351               BY197606.1         1-351
            352-424             BB839524.1         326-398
            425-872             CF172418.1         244-691
            873-1364            BQ572351.1         131-622
            1365-1376           AC136456.3         105057-105068       c
            1377-1973           AK017713.1         1-597
            1974-2898           AC136456.3         99657-100581        c
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 16.84 cM"
     gene            1..2898
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /note="p21 (RAC1) activated kinase 4"
                     /db_xref="GeneID:70584"
                     /db_xref="MGI:MGI:1917834"
     exon            1..150
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            151..224
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            225..489
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     CDS             286..2067
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /EC_number="2.7.11.1"
                     /note="PAK-4; p21-activated kinase 4; p21 protein
                     (Cdc42/Rac)-activated kinase 4"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PAK 4"
                     /protein_id="NP_081746.1"
                     /db_xref="CCDS:CCDS21049.1"
                     /db_xref="GeneID:70584"
                     /db_xref="MGI:MGI:1917834"
                     /translation="MFGKKKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIE
                     ESARRPKPLIDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRR
                     ESPPPPARAHQENGMLEERAAPARMAPDKAGSRARATGHSEAGSGSGDRRRVGPEKRP
                     KSSRDGPGGPQEASRDKRPLSGPDVSTPQPGSLTSGTKLAAGRPFNTYPRADTDHPPR
                     GAQGEPHTMAPNGPSATGLAAPQSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPARA
                     LAAPAVPPAPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGST
                     GIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYRHENVVEMYNSYLVGDE
                     LWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALAVLHAQGVIHRDIKSDSILL
                     THDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGVMVI
                     EMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKASPSLKGFLDRLLVRDPAQRATAA
                     ELLKHPFLTKAGPPASIVPLMRQHRTR"
     exon            490..948
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1389
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1523
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1650
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1776
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1911
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2898
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2873..2878
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /note="hexamer: ATTAAA"
     polyA_site      2896
                     /gene="Pak4"
                     /gene_synonym="5730488L07Rik; mKIAA1142"
                     /note="major polyA site"
ORIGIN      
        1 gagtagggga ggccccggat gttcattggg gattcaacat ggcggcggca gtagccgcgg
       61 cggcggcggc gcgagaccgc tgagggcggt acgcgggcac agagttctgg gccaggcagc
      121 taggccgcga gcgaccgcgg cgccaagccg atgagtaact caaagcctca ggaggagacg
      181 tcacccacgc aaaggtgctt ctagcccagc cagcttcaga ccaggtgcct gtaagaactc
      241 aagccacccc actgttcaca aactctgtgc taaggccctg gaaccatgtt tggaaagaag
      301 aagaagcggg tggagatctc agccccatcc aacttcgagc accgtgtaca cacaggcttt
      361 gatcagcatg agcagaagtt cacagggctg ccccgccagt ggcagagcct gattgaggaa
      421 tcagcacgca gacccaagcc cctgatcgac cctgcctgca tcacgtccat ccagcctgga
      481 gcccccaaga ccatcgttcg gggcagcaaa ggtgccaagg atggggctct cactctgctg
      541 cttgacgaat ttgagaacat gtcggtgacg cgctccaact ccctacgcag agagagtcca
      601 ccaccacctg cccgtgccca ccaggagaat ggaatgctgg aggagcgggc agccccggcc
      661 agaatggccc ctgacaaagc tggaagcaga gcccgggcca caggccacag tgaggcaggc
      721 agtggcagtg gtgacagacg gcgggtgggg ccagagaaaa ggcccaaatc ttcgagggat
      781 ggtccaggag gaccccagga ggcctcccgc gataagcgcc cactctctgg gcctgatgtc
      841 agcactcctc agcctggcag tctgaccagc gggacaaaac tagcagctgg caggcccttt
      901 aacacatacc cacgggctga cacggaccac ccaccccgcg gtgcccaggg ggagccacat
      961 accatggccc ctaatgggcc ttcagccaca ggcctggccg ctcctcagtc ttcctcctcc
     1021 tcccggcctc ccacccgagc ccgtggtgct cccagcccag gagttctggg tccccatgcc
     1081 tctgagcccc agttggcccc cccagcacgt gcccttgctg cccctgctgt acctcctgcc
     1141 cctgggcccc ctgggcctcg ctcaccacag cgggagcccc aacgagtgtc ccatgagcag
     1201 ttccgggctg ccctgcagct ggtggtggac ccaggggacc ctcgttccta tctagacaac
     1261 ttcatcaaga ttggtgaggg ctctacaggt attgtgtgca ttgccactgt acgcagctca
     1321 ggcaaactgg tggccgtcaa gaagatggac ttgcgcaagc agcaaagacg tgaactgctc
     1381 ttcaatgagg tggtgatcat gcgggactac cggcacgaga acgtggtgga gatgtacaac
     1441 agctacctgg tgggtgacga actctgggtc gtcatggagt tcctggaagg cggcgccctc
     1501 acggatattg tcacccacac caggatgaac gaggaacaga tcgccgccgt gtgcctggct
     1561 gtgcttcagg cgctggctgt gctccacgcc cagggtgtca tccaccgtga catcaagagt
     1621 gactctatct tgctgaccca tgatggccgg gtgaagctgt ccgacttcgg gttttgtgcc
     1681 caggtgagca aggaggtgcc tcggaggaag tcgctggtgg gcaccccgta ttggatggcc
     1741 ccggagctca tctcccgcct tccctatggg ccagaggtgg atatctggtc actgggggtg
     1801 atggtgatcg agatggtgga tggggagccc ccttacttca acgagccacc cctcaaagct
     1861 atgaagatga tccgggacaa cctcccgccc cgactgaaga acctgcacaa ggcgtcaccg
     1921 tctctgaaag gcttcttgga tcgcctgcta gtgcgggacc cggcccagcg ggccactgct
     1981 gccgagctgc tgaagcaccc gttcctcacc aaggcggggc caccagccag catcgtgccc
     2041 ctgatgcgcc agcaccgaac cagatgagcc cgagcctagt cttgaaccaa agagcctggc
     2101 tacccttgcc aggcagaggc cagtagaggg ccaaccggct cccacagccc aggagacatt
     2161 ctagggcacc gccgtcactg tgctggggcc ttcgtgggac ctactactga actctggttt
     2221 tgattccatg acttgtaaag ccacaccagg acatgtggag cacatgaggc tcccaggaac
     2281 ccaggcccct cccctgggcg ctgtctgcag gagagaggcg gctgcccatc actggaaatc
     2341 tgcatcaggg gccactggga cagagacgac actatgagaa aggttgtgtg tgtgtgtgtg
     2401 tgtgtgtgtg tgtgtgtgtg tgacagagag agagagagag agagagagag agagtgtgtg
     2461 tgagagagag agagtgtgtg tgagagagag agagcactcg tgcaccaaga aagtccagct
     2521 cctctgtcct cagcctatct ggtgcccagc ccctgaccct gagccattga agggtcaatc
     2581 atgaatgttt gaagagtggc cttttcccag agccccacac cctctttcca caggagaaac
     2641 atggggcctc cccttcccca acctctgcag caaatgacta ctgcacccag acagcctccg
     2701 ttttctagaa gtctatttat attgtcattt tataacacta gctgtgtctc tgtctcagcc
     2761 taggaacaga tggtccctga cctatggggt ggctggccca gggacagggc cactttgaag
     2821 aatcaggctc ctgtccccca ccctgccgcc agtccctcaa gttagtttta caattaaagt
     2881 attttcttgt ttttggtg
//
