LOCUS       NM_001357362            3048 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus p21 (RAC1) activated kinase 1 (Pak1), transcript
            variant 2, mRNA.
ACCESSION   NM_001357362 XM_006507434
VERSION     NM_001357362.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Baudouin,L., Ades,N., Kante,K., Bachelin,C., Hmidan,H., Deboux,C.,
            Panic,R., Ben Messaoud,R., Velut,Y., Hamada,S., Pionneau,C.,
            Duarte,K., Poea-Guyon,S., Barnier,J.V., Nait Oumesmar,B. and
            Bouslama-Oueghlani,L.
  TITLE     Antagonistic actions of PAK1 and NF2/Merlin drive myelin membrane
            expansion in oligodendrocytes
  JOURNAL   Glia 72 (8), 1518-1540 (2024)
   PUBMED   38794866
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Ma,Y., Dumesny,C., Dong,L., Ang,C.S., Asadi,K., Zhan,Y.,
            Nikfarjam,M. and He,H.
  TITLE     Inhibition of P21-activated kinases 1 and 4 synergistically
            suppresses the growth of pancreatic cancer by stimulating
            anti-tumour immunity
  JOURNAL   Cell Commun Signal 22 (1), 287 (2024)
   PUBMED   38797819
  REMARK    GeneRIF: Inhibition of P21-activated kinases 1 and 4
            synergistically suppresses the growth of pancreatic cancer by
            stimulating anti-tumour immunity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3048)
  AUTHORS   Hu,B., Moiseev,D., Schena,I., Faezov,B., Dunbrack,R., Chernoff,J.
            and Li,J.
  TITLE     PAK2 is necessary for myelination in the peripheral nervous system
  JOURNAL   Brain 147 (5), 1809-1821 (2024)
   PUBMED   38079473
REFERENCE   4  (bases 1 to 3048)
  AUTHORS   Chen,M., Pan,L., Chen,D., Wu,Y., Ye,J., Li,K., Zhang,N. and Xu,J.
  TITLE     PAK1 Promotes Inflammation Induced by Sepsis through the
            Snail/CXCL2 Signaling Pathway
  JOURNAL   ACS Infect Dis 10 (4), 1370-1378 (2024)
   PUBMED   38503263
  REMARK    GeneRIF: PAK1 Promotes Inflammation Induced by Sepsis through the
            Snail/CXCL2 Signaling Pathway.
REFERENCE   5  (bases 1 to 3048)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 3048)
  AUTHORS   Vignal,E., De Toledo,M., Comunale,F., Ladopoulou,A.,
            Gauthier-Rouviere,C., Blangy,A. and Fort,P.
  TITLE     Characterization of TCL, a new GTPase of the rho family related to
            TC10 andCcdc42
  JOURNAL   J Biol Chem 275 (46), 36457-36464 (2000)
   PUBMED   10967094
REFERENCE   7  (bases 1 to 3048)
  AUTHORS   Pittoggi,C., Renzi,L., Zaccagnini,G., Cimini,D., Degrassi,F.,
            Giordano,R., Magnano,A.R., Lorenzini,R., Lavia,P. and Spadafora,C.
  TITLE     A fraction of mouse sperm chromatin is organized in nucleosomal
            hypersensitive domains enriched in retroposon DNA
  JOURNAL   J Cell Sci 112 (Pt 20), 3537-3548 (1999)
   PUBMED   10504302
REFERENCE   8  (bases 1 to 3048)
  AUTHORS   Burbelo,P.D., Kozak,C.A., Finegold,A.A., Hall,A. and Pirone,D.M.
  TITLE     Cloning, central nervous system expression and chromosomal mapping
            of the mouse PAK-1 and PAK-3 genes
  JOURNAL   Gene 232 (2), 209-215 (1999)
   PUBMED   10352232
REFERENCE   9  (bases 1 to 3048)
  AUTHORS   Osada,S., Izawa,M., Saito,R., Mizuno,K., Suzuki,A., Hirai,S. and
            Ohno,S.
  TITLE     YSK1, a novel mammalian protein kinase structurally related to
            Ste20 and SPS1, but is not involved in the known MAPK pathways
  JOURNAL   Oncogene 14 (17), 2047-2057 (1997)
   PUBMED   9160885
REFERENCE   10 (bases 1 to 3048)
  AUTHORS   Burbelo,P.D., Drechsel,D. and Hall,A.
  TITLE     A conserved binding motif defines numerous candidate target
            proteins for both Cdc42 and Rac GTPases
  JOURNAL   J Biol Chem 270 (49), 29071-29074 (1995)
   PUBMED   7493928
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC120438.10.
            
            On Mar 31, 2022 this sequence version replaced NM_001357362.1.
            
            Transcript Variant: Variants 2 and 6 encode the same isoform (2).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1356455.1,
                                           SRR7974084.299.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               AC120438.10        46428-46598
            172-385             AC120438.10        57893-58106
            386-486             AC120438.10        68929-69029
            487-634             AC120438.10        69519-69666
            635-672             AC120438.10        75032-75069
            673-789             AC120438.10        86929-87045
            790-813             AC120438.10        88210-88233
            814-988             AC120438.10        89755-89929
            989-1052            AC120438.10        92414-92477
            1053-1101           AC120438.10        97422-97470
            1102-1214           AC120438.10        101982-102094
            1215-1332           AC120438.10        103797-103914
            1333-1432           AC120438.10        107094-107193
            1433-1629           AC120438.10        108095-108291
            1630-1767           AC120438.10        111265-111402
            1768-3048           AC120438.10        114553-115833
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 53.57 cM"
     gene            1..3048
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /note="p21 (RAC1) activated kinase 1"
                     /db_xref="GeneID:18479"
                     /db_xref="MGI:MGI:1339975"
     exon            1..171
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            172..385
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     CDS             196..1854
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine/threonine-protein kinase PAK 1; p65-PAK; alpha-PAK;
                     p21-activated kinase 1; CDC42/RAC effector kinase PAK-A;
                     activated protein kinase alpha; P21 CDKN1A-activated
                     kinase 1; p21 protein (Cdc42/Rac)-activated kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PAK 1 isoform 2"
                     /protein_id="NP_001344291.1"
                     /db_xref="GeneID:18479"
                     /db_xref="MGI:MGI:1339975"
                     /translation="MSNNGVDIQDKPPAPPMRNTSTMIGAGSKDTGTLNHGSKPLPPN
                     PEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQ
                     WARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNTLN
                     VKTVSETPAVPPVSEDEDDDDDATPPPVIAPRPEHTKSFGQKDQREVYTRSVIEPLPV
                     TPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKK
                     YTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNI
                     VNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQV
                     IHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGP
                     KVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRC
                     LEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH"
     exon            386..486
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            487..634
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            635..672
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            673..789
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            790..813
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            814..988
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1052
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1101
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1214
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1332
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1432
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1629
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1767
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..3048
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1896..1901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /note="hexamer: AATAAA"
     polyA_site      1929
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
     regulatory      3029..3034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /note="hexamer: AATAAA"
     polyA_site      3048
                     /gene="Pak1"
                     /gene_synonym="PAK-1; Paka"
                     /note="major polyA site"
ORIGIN      
        1 gagtgtgctg cgccttggga gctgggtggc tgcgctgcgg cgcttagccg aatccagcct
       61 gtcaggctgg ttcagcccgg gctttgtccc aactgctgag cagggagagg tgcggctctg
      121 cgacagatac acaagatcat cagagatgct gggccccgga cccaccgcca gtagctgctg
      181 ctgctggtgg tgacaatgtc aaataacggc gtagacatcc aggacaaacc cccagcccct
      241 ccgatgagaa acaccagcac tatgattgga gccggcagca aagacactgg aaccctaaac
      301 cacggctcca aacctctgcc tccaaaccca gaggagaaga aaaagaagga ccggttttat
      361 cgatccatct tacctggaga taaaacaaat aaaaagaagg agaaggagcg accagagatt
      421 tctcttcctt cagattttga gcatacaatt catgttggtt ttgatgctgt cacaggggag
      481 tttacgggaa tgccagaaca gtgggctcgc ttgcttcaaa catcaaatat cacaaagtca
      541 gaacagaaga aaaacccaca ggctgttctg gatgtgttgg aattttataa ctctaagaag
      601 acctccaata gtcagaagta catgagtttt acagataagt cagctgaaga ttataattct
      661 tctaacactt tgaatgtgaa gactgtgtct gagaccccag cagtaccacc agtgtcagaa
      721 gatgaagatg atgatgacga tgctacccca cctccagtga ttgctccacg cccagaacac
      781 acaaaatctt ttgggcagaa ggatcagaga gaggtatata cacgatctgt gattgaacca
      841 cttcctgtta ctccaactcg ggatgtggct acatctccta tttctcctac tgagaataac
      901 accactccgc cagatgcttt gacccggaac acggaaaaac agaagaagaa gcctaaaatg
      961 tctgatgagg agatcttaga gaaattacgg agcatagtga gtgtgggtga tcctaagaag
     1021 aagtacacac ggttcgagaa gattggacaa ggtgcttcag gcacagtgta tactgcaatg
     1081 gatgtagcca cagggcagga ggtggccatt aaacagatga atcttcagca gcagccgaag
     1141 aaagagctga ttattaatga gatcctggtc atgagggaaa acaaaaaccc aaatattgtc
     1201 aactacctgg acagttacct tgtgggagat gagctgtggg ttgttatgga atacttggct
     1261 ggaggctcct tgacagatgt ggtgacagaa acctgtatgg atgaaggcca gatagcagct
     1321 gtgtgccgag agtgtctaca agctttggag tttctacatt caaaccaagt cattcacagg
     1381 gacatcaaga gtgacaatat tctgctggga atggatggct ctgtcaagtt aactgacttt
     1441 ggattctgtg cacagataac tccagagcag agcaaaagga gcaccatggt gggaactcca
     1501 tattggatgg cacctgaagt tgtgacacgc aaggcctatg gacccaaggt tgacatctgg
     1561 tccctgggca ttatggcaat tgaaatgatt gagggggagc ccccatacct caatgaaaac
     1621 cctttgagag ccttgtacct cattgctacc aatgggacgc cagagcttca gaacccagag
     1681 aagttgtcag ctattttccg ggacttcctg aaccgctgtc ttgagatgga tgtggagaag
     1741 agaggctcag ctaaagagct gctgcagcat cagttcctga agattgccaa gcccctctct
     1801 agcctgactc cactgattgc tgcagcaaaa gaggcaacca agaacaatca ctgaaaccac
     1861 gctcatccca gcctcatgtg ccaagccttc tatgaaataa acacttgttt caggaactcc
     1921 gacccctcat gtcctcttct cctttccttg cttctcccat ttcctgatct agtgctccca
     1981 agactttgat ccttggaaac tgtctagcac tgaagagaat tgcaactgga cgagtaatcc
     2041 agcagaggcc atttctaaat aggaattccc ttccagtttg tgggcatgga gggaactgat
     2101 gagcaagggt ttacccgaat aaacctgttt ctacgaaaca gaatctcagc catcccattc
     2161 cttacccctc acaatcagtt cttaactata taaacttttg gtttgatagc attatcgatt
     2221 tgagatcagt tgaaattgct ttactttttt atttctgtga ctaaattgcc tgaacacctc
     2281 attgtatttg aaaaccataa cagctttgaa atgccacggg gcctgataat ctgccaggga
     2341 caggaagagg ctctgtttcc cgaaccatgc ccttggtgca gctgatcctg acatgggaga
     2401 acgaccatgt ttttctttgt gtgtgcttct agcagctgtt ttggagaacc atgacccagt
     2461 agtgttccct gtgctatttc tagtgaaatg gtcttagcta tgtagcagct tttgattccc
     2521 tgcatcccct aggctgctgc ttgcattctg tccttattta tagcattgag agatttccta
     2581 gagcacatgc tgagtaagag aagtgtgaga agtcagaggg aaagtgcagc tacttttagc
     2641 acatggctgg cttcagcacc tgtccggcta tacttgtgtg atatttcaag atctcagcct
     2701 ctgtttctac ctgggactca gatcttctgg tagcttaact atttcagtcg aaagagaagg
     2761 tctctaaatc cgagatgatg tgctactagt aataagggga ccctgttttt aagtgcatga
     2821 ccaagctctc tgcaagtgga catgcttact ctctgcaaat ggacgcgtat tgcgggtgtt
     2881 tgctacctcc tgccctcctc atggttttgg ttctcctgct gtggtaggat gcttggccag
     2941 cattgtggct tgtcatgcca gccccattgc ctaccttgtc atgctcagag gtcctactgc
     3001 ctcagcagca caagtttcta tttccttcaa taaaaggaga tgaaaata
//
