LOCUS       NM_025939               2509 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphoribosylaminoimidazole carboxylase,
            phosphoribosylaminoribosylaminoimidazole, succinocarboxamide
            synthetase (Paics), transcript variant 1, mRNA.
ACCESSION   NM_025939
VERSION     NM_025939.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Mizukoshi,T., Yamada,S. and Sakakibara,S.I.
  TITLE     Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis
            during Brain Development
  JOURNAL   eNeuro 10 (10) (2023)
   PUBMED   37770184
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2509)
  AUTHORS   Baresova,V., Krijt,M., Skopova,V., Souckova,O., Kmoch,S. and
            Zikanova,M.
  TITLE     CRISPR-Cas9 induced mutations along de novo purine synthesis in
            HeLa cells result in accumulation of individual enzyme substrates
            and affect purinosome formation
  JOURNAL   Mol Genet Metab 119 (3), 270-277 (2016)
   PUBMED   27590927
REFERENCE   4  (bases 1 to 2509)
  AUTHORS   Lastwika,K.J., Wilson,W. 3rd, Li,Q.K., Norris,J., Xu,H.,
            Ghazarian,S.R., Kitagawa,H., Kawabata,S., Taube,J.M., Yao,S.,
            Liu,L.N., Gills,J.J. and Dennis,P.A.
  TITLE     Control of PD-L1 Expression by Oncogenic Activation of the AKT-mTOR
            Pathway in Non-Small Cell Lung Cancer
  JOURNAL   Cancer Res 76 (2), 227-238 (2016)
   PUBMED   26637667
REFERENCE   5  (bases 1 to 2509)
  AUTHORS   Combes,A.N., Lesieur,E., Harley,V.R., Sinclair,A.H., Little,M.H.,
            Wilhelm,D. and Koopman,P.
  TITLE     Three-dimensional visualization of testis cord morphogenesis, a
            novel tubulogenic mechanism in development
  JOURNAL   Dev Dyn 238 (5), 1033-1041 (2009)
   PUBMED   19334288
REFERENCE   6  (bases 1 to 2509)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   7  (bases 1 to 2509)
  AUTHORS   Lepourcelet,M., Tou,L., Cai,L., Sawada,J., Lazar,A.J.,
            Glickman,J.N., Williamson,J.A., Everett,A.D., Redston,M., Fox,E.A.,
            Nakatani,Y. and Shivdasani,R.A.
  TITLE     Insights into developmental mechanisms and cancers in the mammalian
            intestine derived from serial analysis of gene expression and study
            of the hepatoma-derived growth factor (HDGF)
  JOURNAL   Development 132 (2), 415-427 (2005)
   PUBMED   15604097
REFERENCE   8  (bases 1 to 2509)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   9  (bases 1 to 2509)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2509)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114666.31, BC052691.1 and BP772410.1.
            
            On Oct 14, 2017 this sequence version replaced NM_025939.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a). Variants 1-4 all
            encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052691.1, AK159398.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-109               AC114666.31        82143-82251         c
            110-2487            BC052691.1         1-2378
            2488-2509           BP772410.1         26-47               c
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 41.45 cM"
     gene            1..2509
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="phosphoribosylaminoimidazole carboxylase,
                     phosphoribosylaminoribosylaminoimidazole,
                     succinocarboxamide synthetase"
                     /db_xref="GeneID:67054"
                     /db_xref="MGI:MGI:1914304"
     exon            1..534
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     CDS             519..1796
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /EC_number="6.3.2.6"
                     /EC_number="4.1.1.21"
                     /note="isoform a is encoded by transcript variant 1;
                     multifunctional protein ADE2; bifunctional
                     phosphoribosylaminoimidazole
                     carboxylase/phosphoribosylaminoimidazole
                     succinocarboxamide synthetase"
                     /codon_start=1
                     /product="bifunctional phosphoribosylaminoimidazole
                     carboxylase/phosphoribosylaminoimidazole
                     succinocarboxamide synthetase isoform a"
                     /protein_id="NP_080215.1"
                     /db_xref="CCDS:CCDS19367.1"
                     /db_xref="GeneID:67054"
                     /db_xref="MGI:MGI:1914304"
                     /translation="MATAVVVNIGKKLYEGKTKEVYELLDTPGRVLLQSKDQITAGNA
                     ARKNHLEGKAAISNKITSCIFQLLQEAGIKTAFTKKCGETAFIAPQCEMIPIEWVCRR
                     IATGSFLKRNPGVQEGYKFYPPKVEMFFKDDANNDPQWSEEQLIAAKFCFAGLVIGQT
                     EVDIMSHATQAIFEILEKSWLPQDCTLVDMKIEFGVDVTTKEIVLADVIDNDSWRLWP
                     SGDRSQQKDKQSYRDLKEVTPEGLQMVKKNFEWVADRVELLLKSDSQCRVVVLMGSTS
                     DLGHCEKIKKACGNFGIPCELRVTSAHKGPDETLRIKAEYEGDGIPTVFVSVAGRSNG
                     LGPVLSGNTAYPVISCPPITPDWGAQDVWSSLRLPSGIGCSTILSPEGSAQFAAQIFG
                     LNNHLVWAKLRASILNTWISLKQADKKVRQCNL"
     exon            535..732
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            733..911
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1091
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1205
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1289
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1470
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1629
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..2491
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2465..2470
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="hexamer: ATTAAA"
     polyA_site      2489
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="major polyA site"
ORIGIN      
        1 gggcggggct acggagtcaa agaggtgggg aggaggccga ggtcaggatc gctactttct
       61 ctgcacgtgg gaggctccgt tattttcccg cgcccaagga gtaggtgatg agagtggcgc
      121 agctgatcgc gtgggtccct cagaaaacct gagccgttcg tgccccctgg tctcacgccg
      181 tcccccggcc gtcaggactt ggtccgctcc aacccagaaa cctctccggc cagcgggggc
      241 cttgcagcat gcgcgccact ttttggctgc ctgcggggag gggccgcccg gaagcggggt
      301 cgcgtcagcg cgcctgcgcg gcgggaagcc tggggacacg actccagccc caagcacagc
      361 cagcccggta cttcgggttc gccggtgggc tgtggggcta gggcgtgtcc ttgatattgc
      421 acctcccttt cccgccctct gatcctgtgg tcgcccttgt tttccagaac actgcccttt
      481 tttcccgcgc ggtctgctcc tctagttcac ctcggataat ggcgacagcc gtagtagtga
      541 acatcgggaa gaagctctac gagggtaaaa caaaagaagt ctatgagttg ttagacaccc
      601 caggcagagt cctcctgcag tccaaggacc agatcacagc agggaatgca gctaggaaga
      661 accacctgga aggcaaagcc gccatctcca acaagatcac cagctgcata tttcagttgt
      721 tacaggaagc tggtataaaa actgctttca ccaagaaatg cggggagact gctttcattg
      781 caccccagtg tgaaatgatc cccattgagt gggtgtgccg aagaatagca actgggtctt
      841 tcctcaaaag gaaccctggg gtacaggagg ggtacaagtt ctatccaccc aaagtggaga
      901 tgttcttcaa ggacgatgcc aataatgatc cacagtggtc tgaggagcaa ctcattgctg
      961 caaaattctg ttttgctggc cttgttatag ggcagactga agtggacatc atgagtcatg
     1021 ccacacaagc tatttttgaa attctggaga aatcctggct gccccaggac tgtacgctgg
     1081 ttgatatgaa gattgaattt ggtgttgatg taaccaccaa agagattgtt cttgctgatg
     1141 tcattgataa tgattcctgg agactatggc catcgggaga tcggagccag cagaaggaca
     1201 aacagtctta ccgtgatctc aaagaagtaa ctccagaagg actccagatg gtaaagaaaa
     1261 actttgagtg ggttgcagac cgagtggagt tgcttctcaa gtcagacagt cagtgcaggg
     1321 tggtagtgct gatgggctcc acctctgacc ttggtcactg tgagaaaatt aagaaggctt
     1381 gtggaaattt tgggattcca tgtgaactta gagtaacatc tgcgcataaa ggaccagatg
     1441 agactttgag gattaaagca gaatatgaag gggatggcat acctactgta tttgtctctg
     1501 tggcgggcag aagcaatggt ctaggcccag tgctgtctgg caatactgca tatccggtta
     1561 tcagctgtcc cccgatcaca ccagactggg gcgctcagga tgtgtggtca tctcttcgac
     1621 tgcccagtgg tattggctgt tcaactatac tttctccaga aggatcagct cagtttgctg
     1681 ctcagatatt tgggttaaac aatcatttgg tgtgggccaa acttcgagcg agcatattaa
     1741 acacgtggat atccttaaag caggctgata agaaggtcag acaatgcaat ttgtaaagaa
     1801 gcaatttaat ttttaggaga aaagttacgt agtcctagtt tggatttaaa gaaaaaagtc
     1861 aagcaaaatg atttcacata agagaaaaca atttagttac taatgaacaa atgttactca
     1921 gtgtttatga gttggcacat tgtcagtgga tattactaat atttctagct ctacaaagtg
     1981 atatgtccct gtaaccttag cactccagag gctgaggcag cagcaggacc tcttaagttc
     2041 agggccaccc tgagctgtgt attgagactc aacttgccgt ggagggaatc tttctagcta
     2101 gtaaaattgg catgacacta ctaaatttat tctcacgctc tgtatagtca ctgttgggta
     2161 aatatgtaac tatggaactg gaacaatgtg tctgctgtct gaaccactat gaagaaccag
     2221 tgtggctctc ttgcagacga cctgaatttg cttcccagca tctctgtaga ctgtttcaca
     2281 ataaataccc tttaaatcag gggatctgac cccatggctt cctcatgcac ctgctttcaa
     2341 atactattac attcccccac ccaacacaca caattttaaa aagtaaactt tttaaaaaga
     2401 gtaatagcat tttttgttgt tgttaatcag tagataatgc ttgaactgag ttgatacatt
     2461 gaaaattaaa atacttgtgt ataacaatat aaaaaaaaaa aaaaaaaaa
//
