LOCUS       NM_001356972            2124 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphoribosylaminoimidazole carboxylase,
            phosphoribosylaminoribosylaminoimidazole, succinocarboxamide
            synthetase (Paics), transcript variant 3, mRNA.
ACCESSION   NM_001356972 XM_006535181
VERSION     NM_001356972.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Mizukoshi,T., Yamada,S. and Sakakibara,S.I.
  TITLE     Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis
            during Brain Development
  JOURNAL   eNeuro 10 (10) (2023)
   PUBMED   37770184
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2124)
  AUTHORS   Baresova,V., Krijt,M., Skopova,V., Souckova,O., Kmoch,S. and
            Zikanova,M.
  TITLE     CRISPR-Cas9 induced mutations along de novo purine synthesis in
            HeLa cells result in accumulation of individual enzyme substrates
            and affect purinosome formation
  JOURNAL   Mol Genet Metab 119 (3), 270-277 (2016)
   PUBMED   27590927
REFERENCE   4  (bases 1 to 2124)
  AUTHORS   Lastwika,K.J., Wilson,W. 3rd, Li,Q.K., Norris,J., Xu,H.,
            Ghazarian,S.R., Kitagawa,H., Kawabata,S., Taube,J.M., Yao,S.,
            Liu,L.N., Gills,J.J. and Dennis,P.A.
  TITLE     Control of PD-L1 Expression by Oncogenic Activation of the AKT-mTOR
            Pathway in Non-Small Cell Lung Cancer
  JOURNAL   Cancer Res 76 (2), 227-238 (2016)
   PUBMED   26637667
REFERENCE   5  (bases 1 to 2124)
  AUTHORS   Combes,A.N., Lesieur,E., Harley,V.R., Sinclair,A.H., Little,M.H.,
            Wilhelm,D. and Koopman,P.
  TITLE     Three-dimensional visualization of testis cord morphogenesis, a
            novel tubulogenic mechanism in development
  JOURNAL   Dev Dyn 238 (5), 1033-1041 (2009)
   PUBMED   19334288
REFERENCE   6  (bases 1 to 2124)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   7  (bases 1 to 2124)
  AUTHORS   Lepourcelet,M., Tou,L., Cai,L., Sawada,J., Lazar,A.J.,
            Glickman,J.N., Williamson,J.A., Everett,A.D., Redston,M., Fox,E.A.,
            Nakatani,Y. and Shivdasani,R.A.
  TITLE     Insights into developmental mechanisms and cancers in the mammalian
            intestine derived from serial analysis of gene expression and study
            of the hepatoma-derived growth factor (HDGF)
  JOURNAL   Development 132 (2), 415-427 (2005)
   PUBMED   15604097
REFERENCE   8  (bases 1 to 2124)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   9  (bases 1 to 2124)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2124)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114666.31.
            
            On Oct 13, 2017 this sequence version replaced XM_006535181.3.
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1-4 all encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.173293.1,
                                           SRR7345562.159240.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AC114666.31        82152-82251         c
            101-167             AC114666.31        81718-81784         c
            168-365             AC114666.31        76810-77007         c
            366-544             AC114666.31        74060-74238         c
            545-724             AC114666.31        72264-72443         c
            725-838             AC114666.31        72047-72160         c
            839-922             AC114666.31        71043-71126         c
            923-1103            AC114666.31        68931-69111         c
            1104-1262           AC114666.31        67148-67306         c
            1263-2124           AC114666.31        66044-66905         c
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 41.45 cM"
     gene            1..2124
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="phosphoribosylaminoimidazole carboxylase,
                     phosphoribosylaminoribosylaminoimidazole,
                     succinocarboxamide synthetase"
                     /db_xref="GeneID:67054"
                     /db_xref="MGI:MGI:1914304"
     exon            1..100
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            101..167
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..1429
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /EC_number="6.3.2.6"
                     /EC_number="4.1.1.21"
                     /note="isoform a is encoded by transcript variant 3;
                     multifunctional protein ADE2; bifunctional
                     phosphoribosylaminoimidazole
                     carboxylase/phosphoribosylaminoimidazole
                     succinocarboxamide synthetase"
                     /codon_start=1
                     /product="bifunctional phosphoribosylaminoimidazole
                     carboxylase/phosphoribosylaminoimidazole
                     succinocarboxamide synthetase isoform a"
                     /protein_id="NP_001343901.1"
                     /db_xref="CCDS:CCDS19367.1"
                     /db_xref="GeneID:67054"
                     /db_xref="MGI:MGI:1914304"
                     /translation="MATAVVVNIGKKLYEGKTKEVYELLDTPGRVLLQSKDQITAGNA
                     ARKNHLEGKAAISNKITSCIFQLLQEAGIKTAFTKKCGETAFIAPQCEMIPIEWVCRR
                     IATGSFLKRNPGVQEGYKFYPPKVEMFFKDDANNDPQWSEEQLIAAKFCFAGLVIGQT
                     EVDIMSHATQAIFEILEKSWLPQDCTLVDMKIEFGVDVTTKEIVLADVIDNDSWRLWP
                     SGDRSQQKDKQSYRDLKEVTPEGLQMVKKNFEWVADRVELLLKSDSQCRVVVLMGSTS
                     DLGHCEKIKKACGNFGIPCELRVTSAHKGPDETLRIKAEYEGDGIPTVFVSVAGRSNG
                     LGPVLSGNTAYPVISCPPITPDWGAQDVWSSLRLPSGIGCSTILSPEGSAQFAAQIFG
                     LNNHLVWAKLRASILNTWISLKQADKKVRQCNL"
     exon            168..365
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            366..544
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            545..724
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            725..838
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            839..922
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1103
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1262
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..2124
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2098..2103
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="hexamer: ATTAAA"
     polyA_site      2122
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="major polyA site"
ORIGIN      
        1 gggcggggct acggagtcaa agaggtgggg aggaggccga ggtcaggatc gctactttct
       61 ctgcacgtgg gaggctccgt tattttcccg cgcccaagga aacactgccc ttttttcccg
      121 cgcggtctgc tcctctagtt cacctcggat aatggcgaca gccgtagtag tgaacatcgg
      181 gaagaagctc tacgagggta aaacaaaaga agtctatgag ttgttagaca ccccaggcag
      241 agtcctcctg cagtccaagg accagatcac agcagggaat gcagctagga agaaccacct
      301 ggaaggcaaa gccgccatct ccaacaagat caccagctgc atatttcagt tgttacagga
      361 agctggtata aaaactgctt tcaccaagaa atgcggggag actgctttca ttgcacccca
      421 gtgtgaaatg atccccattg agtgggtgtg ccgaagaata gcaactgggt ctttcctcaa
      481 aaggaaccct ggggtacagg aggggtacaa gttctatcca cccaaagtgg agatgttctt
      541 caaggacgat gccaataatg atccacagtg gtctgaggag caactcattg ctgcaaaatt
      601 ctgttttgct ggccttgtta tagggcagac tgaagtggac atcatgagtc atgccacaca
      661 agctattttt gaaattctgg agaaatcctg gctgccccag gactgtacgc tggttgatat
      721 gaagattgaa tttggtgttg atgtaaccac caaagagatt gttcttgctg atgtcattga
      781 taatgattcc tggagactat ggccatcggg agatcggagc cagcagaagg acaaacagtc
      841 ttaccgtgat ctcaaagaag taactccaga aggactccag atggtaaaga aaaactttga
      901 gtgggttgca gaccgagtgg agttgcttct caagtcagac agtcagtgca gggtggtagt
      961 gctgatgggc tccacctctg accttggtca ctgtgagaaa attaagaagg cttgtggaaa
     1021 ttttgggatt ccatgtgaac ttagagtaac atctgcgcat aaaggaccag atgagacttt
     1081 gaggattaaa gcagaatatg aaggggatgg catacctact gtatttgtct ctgtggcggg
     1141 cagaagcaat ggtctaggcc cagtgctgtc tggcaatact gcatatccgg ttatcagctg
     1201 tcccccgatc acaccagact ggggcgctca ggatgtgtgg tcatctcttc gactgcccag
     1261 tggtattggc tgttcaacta tactttctcc agaaggatca gctcagtttg ctgctcagat
     1321 atttgggtta aacaatcatt tggtgtgggc caaacttcga gcgagcatat taaacacgtg
     1381 gatatcctta aagcaggctg ataagaaggt cagacaatgc aatttgtaaa gaagcaattt
     1441 aatttttagg agaaaagtta cgtagtccta gtttggattt aaagaaaaaa gtcaagcaaa
     1501 atgatttcac ataagagaaa acaatttagt tactaatgaa caaatgttac tcagtgttta
     1561 tgagttggca cattgtcagt ggatattact aatatttcta gctctacaaa gtgatatgtc
     1621 cctgtaacct tagcactcca gaggctgagg cagcagcagg acctcttaag ttcagggcca
     1681 ccctgagctg tgtattgaga ctcaacttgc cgtggaggga atctttctag ctagtaaaat
     1741 tggcatgaca ctactaaatt tattctcacg ctctgtatag tcactgttgg gtaaatatgt
     1801 aactatggaa ctggaacaat gtgtctgctg tctgaaccac tatgaagaac cagtgtggct
     1861 ctcttgcaga cgacctgaat ttgcttccca gcatctctgt agactgtttc acaataaata
     1921 ccctttaaat caggggatct gaccccatgg cttcctcatg cacctgcttt caaatactat
     1981 tacattcccc cacccaacac acacaatttt aaaaagtaaa ctttttaaaa agagtaatag
     2041 cattttttgt tgttgttaat cagtagataa tgcttgaact gagttgatac attgaaaatt
     2101 aaaatacttg tgtataacaa tata
//
