LOCUS       NM_001356971            2409 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phosphoribosylaminoimidazole carboxylase,
            phosphoribosylaminoribosylaminoimidazole, succinocarboxamide
            synthetase (Paics), transcript variant 2, mRNA.
ACCESSION   NM_001356971 XM_006535180
VERSION     NM_001356971.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Mizukoshi,T., Yamada,S. and Sakakibara,S.I.
  TITLE     Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis
            during Brain Development
  JOURNAL   eNeuro 10 (10) (2023)
   PUBMED   37770184
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2409)
  AUTHORS   Baresova,V., Krijt,M., Skopova,V., Souckova,O., Kmoch,S. and
            Zikanova,M.
  TITLE     CRISPR-Cas9 induced mutations along de novo purine synthesis in
            HeLa cells result in accumulation of individual enzyme substrates
            and affect purinosome formation
  JOURNAL   Mol Genet Metab 119 (3), 270-277 (2016)
   PUBMED   27590927
REFERENCE   4  (bases 1 to 2409)
  AUTHORS   Lastwika,K.J., Wilson,W. 3rd, Li,Q.K., Norris,J., Xu,H.,
            Ghazarian,S.R., Kitagawa,H., Kawabata,S., Taube,J.M., Yao,S.,
            Liu,L.N., Gills,J.J. and Dennis,P.A.
  TITLE     Control of PD-L1 Expression by Oncogenic Activation of the AKT-mTOR
            Pathway in Non-Small Cell Lung Cancer
  JOURNAL   Cancer Res 76 (2), 227-238 (2016)
   PUBMED   26637667
REFERENCE   5  (bases 1 to 2409)
  AUTHORS   Combes,A.N., Lesieur,E., Harley,V.R., Sinclair,A.H., Little,M.H.,
            Wilhelm,D. and Koopman,P.
  TITLE     Three-dimensional visualization of testis cord morphogenesis, a
            novel tubulogenic mechanism in development
  JOURNAL   Dev Dyn 238 (5), 1033-1041 (2009)
   PUBMED   19334288
REFERENCE   6  (bases 1 to 2409)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   7  (bases 1 to 2409)
  AUTHORS   Lepourcelet,M., Tou,L., Cai,L., Sawada,J., Lazar,A.J.,
            Glickman,J.N., Williamson,J.A., Everett,A.D., Redston,M., Fox,E.A.,
            Nakatani,Y. and Shivdasani,R.A.
  TITLE     Insights into developmental mechanisms and cancers in the mammalian
            intestine derived from serial analysis of gene expression and study
            of the hepatoma-derived growth factor (HDGF)
  JOURNAL   Development 132 (2), 415-427 (2005)
   PUBMED   15604097
REFERENCE   8  (bases 1 to 2409)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   9  (bases 1 to 2409)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2409)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114666.31, AK146722.1 and BP772410.1.
            
            On Oct 13, 2017 this sequence version replaced XM_006535180.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1-4 all encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146722.1, SRR1660819.19762.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AC114666.31        82194-82251         c
            59-2385             AK146722.1         2-2328
            2386-2409           BP772410.1         26-49               c
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 41.45 cM"
     gene            1..2409
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="phosphoribosylaminoimidazole carboxylase,
                     phosphoribosylaminoribosylaminoimidazole,
                     succinocarboxamide synthetase"
                     /db_xref="GeneID:67054"
                     /db_xref="MGI:MGI:1914304"
     exon            1..367
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            368..434
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     CDS             419..1696
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /EC_number="6.3.2.6"
                     /EC_number="4.1.1.21"
                     /note="isoform a is encoded by transcript variant 2;
                     multifunctional protein ADE2; bifunctional
                     phosphoribosylaminoimidazole
                     carboxylase/phosphoribosylaminoimidazole
                     succinocarboxamide synthetase"
                     /codon_start=1
                     /product="bifunctional phosphoribosylaminoimidazole
                     carboxylase/phosphoribosylaminoimidazole
                     succinocarboxamide synthetase isoform a"
                     /protein_id="NP_001343900.1"
                     /db_xref="CCDS:CCDS19367.1"
                     /db_xref="GeneID:67054"
                     /db_xref="MGI:MGI:1914304"
                     /translation="MATAVVVNIGKKLYEGKTKEVYELLDTPGRVLLQSKDQITAGNA
                     ARKNHLEGKAAISNKITSCIFQLLQEAGIKTAFTKKCGETAFIAPQCEMIPIEWVCRR
                     IATGSFLKRNPGVQEGYKFYPPKVEMFFKDDANNDPQWSEEQLIAAKFCFAGLVIGQT
                     EVDIMSHATQAIFEILEKSWLPQDCTLVDMKIEFGVDVTTKEIVLADVIDNDSWRLWP
                     SGDRSQQKDKQSYRDLKEVTPEGLQMVKKNFEWVADRVELLLKSDSQCRVVVLMGSTS
                     DLGHCEKIKKACGNFGIPCELRVTSAHKGPDETLRIKAEYEGDGIPTVFVSVAGRSNG
                     LGPVLSGNTAYPVISCPPITPDWGAQDVWSSLRLPSGIGCSTILSPEGSAQFAAQIFG
                     LNNHLVWAKLRASILNTWISLKQADKKVRQCNL"
     exon            435..632
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            633..811
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            812..991
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1105
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1189
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1370
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1529
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..2391
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2365..2370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="hexamer: ATTAAA"
     polyA_site      2389
                     /gene="Paics"
                     /gene_synonym="2610511I09Rik; ADE2H1; AIRC; PAIS"
                     /note="major polyA site"
ORIGIN      
        1 gggcggggct acggagtcaa agaggtgggg aggaggccga ggtcaggatc gctactttct
       61 ctgcacgtgg gaggctccgt tattttcccg cgcccaagga gtaggtgatg agagtggcgc
      121 agctgatcgc gtgggtccct cagaaaacct gagccgttcg tgccccctgg tctcacgccg
      181 tcccccggcc gtcaggactt ggtccgctcc aacccagaaa cctctccggc cagcgggggc
      241 cttgcagcat gcgcgccact ttttggctgc ctgcggggag gggccgcccg gaagcggggt
      301 cgcgtcagcg cgcctgcgcg gcgggaagcc tggggacacg actccagccc caagcacagc
      361 cagcccgaac actgcccttt tttcccgcgc ggtctgctcc tctagttcac ctcggataat
      421 ggcgacagcc gtagtagtga acatcgggaa gaagctctac gagggtaaaa caaaagaagt
      481 ctatgagttg ttagacaccc caggcagagt cctcctgcag tccaaggacc agatcacagc
      541 agggaatgca gctaggaaga accacctgga aggcaaagcc gccatctcca acaagatcac
      601 cagctgcata tttcagttgt tacaggaagc tggtataaaa actgctttca ccaagaaatg
      661 cggggagact gctttcattg caccccagtg tgaaatgatc cccattgagt gggtgtgccg
      721 aagaatagca actgggtctt tcctcaaaag gaaccctggg gtacaggagg ggtacaagtt
      781 ctatccaccc aaagtggaga tgttcttcaa ggacgatgcc aataatgatc cacagtggtc
      841 tgaggagcaa ctcattgctg caaaattctg ttttgctggc cttgttatag ggcagactga
      901 agtggacatc atgagtcatg ccacacaagc tatttttgaa attctggaga aatcctggct
      961 gccccaggac tgtacgctgg ttgatatgaa gattgaattt ggtgttgatg taaccaccaa
     1021 agagattgtt cttgctgatg tcattgataa tgattcctgg agactatggc catcgggaga
     1081 tcggagccag cagaaggaca aacagtctta ccgtgatctc aaagaagtaa ctccagaagg
     1141 actccagatg gtaaagaaaa actttgagtg ggttgcagac cgagtggagt tgcttctcaa
     1201 gtcagacagt cagtgcaggg tggtagtgct gatgggctcc acctctgacc ttggtcactg
     1261 tgagaaaatt aagaaggctt gtggaaattt tgggattcca tgtgaactta gagtaacatc
     1321 tgcgcataaa ggaccagatg agactttgag gattaaagca gaatatgaag gggatggcat
     1381 acctactgta tttgtctctg tggcgggcag aagcaatggt ctaggcccag tgctgtctgg
     1441 caatactgca tatccggtta tcagctgtcc cccgatcaca ccagactggg gcgctcagga
     1501 tgtgtggtca tctcttcgac tgcccagtgg tattggctgt tcaactatac tttctccaga
     1561 aggatcagct cagtttgctg ctcagatatt tgggttaaac aatcatttgg tgtgggccaa
     1621 acttcgagcg agcatattaa acacgtggat atccttaaag caggctgata agaaggtcag
     1681 acaatgcaat ttgtaaagaa gcaatttaat ttttaggaga aaagttacgt agtcctagtt
     1741 tggatttaaa gaaaaaagtc aagcaaaatg atttcacata agagaaaaca atttagttac
     1801 taatgaacaa atgttactca gtgtttatga gttggcacat tgtcagtgga tattactaat
     1861 atttctagct ctacaaagtg atatgtccct gtaaccttag cactccagag gctgaggcag
     1921 cagcaggacc tcttaagttc agggccaccc tgagctgtgt attgagactc aacttgccgt
     1981 ggagggaatc tttctagcta gtaaaattgg catgacacta ctaaatttat tctcacgctc
     2041 tgtatagtca ctgttgggta aatatgtaac tatggaactg gaacaatgtg tctgctgtct
     2101 gaaccactat gaagaaccag tgtggctctc ttgcagacga cctgaatttg cttcccagca
     2161 tctctgtaga ctgtttcaca ataaataccc tttaaatcag gggatctgac cccatggctt
     2221 cctcatgcac ctgctttcaa atactattac attcccccac ccaacacaca caattttaaa
     2281 aagtaaactt tttaaaaaga gtaatagcat tttttgttgt tgttaatcag tagataatgc
     2341 ttgaactgag ttgatacatt gaaaattaaa atacttgtgt ataacaatat aaaaaaaaaa
     2401 aaaaaaaaa
//
