LOCUS       NM_001285874            3136 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus platelet-activating factor acetylhydrolase 2 (Pafah2),
            transcript variant 3, mRNA.
ACCESSION   NM_001285874
VERSION     NM_001285874.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Taketomi,Y., Higashi,T., Kano,K., Miki,Y., Mochizuki,C.,
            Toyoshima,S., Okayama,Y., Nishito,Y., Nakae,S., Tanaka,S.,
            Tokuoka,S.M., Oda,Y., Shichino,S., Ueha,S., Matsushima,K.,
            Akahoshi,N., Ishii,S., Chun,J., Aoki,J. and Murakami,M.
  TITLE     Lipid-orchestrated paracrine circuit coordinates mast cell
            maturation and anaphylaxis through functional interaction with
            fibroblasts
  JOURNAL   Immunity 57 (8), 1828-1847 (2024)
   PUBMED   39002541
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Moriyama,H., Endo,J., Kataoka,M., Shimanaka,Y., Kono,N.,
            Sugiura,Y., Goto,S., Kitakata,H., Hiraide,T., Yoshida,N., Isobe,S.,
            Yamamoto,T., Shirakawa,K., Anzai,A., Katsumata,Y., Suematsu,M.,
            Kosaki,K., Fukuda,K., Arai,H. and Sano,M.
  TITLE     Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells
            regulate pulmonary vascular remodeling
  JOURNAL   Nat Commun 13 (1), 3013 (2022)
   PUBMED   35641514
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3136)
  AUTHORS   Shimanaka,Y., Kono,N., Taketomi,Y., Arita,M., Okayama,Y.,
            Tanaka,Y., Nishito,Y., Mochizuki,T., Kusuhara,H., Adibekian,A.,
            Cravatt,B.F., Murakami,M. and Arai,H.
  TITLE     Omega-3 fatty acid epoxides are autocrine mediators that control
            the magnitude of IgE-mediated mast cell activation
  JOURNAL   Nat Med 23 (11), 1287-1297 (2017)
   PUBMED   29035365
REFERENCE   4  (bases 1 to 3136)
  AUTHORS   Kono,N., Inoue,T., Yoshida,Y., Sato,H., Matsusue,T., Itabe,H.,
            Niki,E., Aoki,J. and Arai,H.
  TITLE     Protection against oxidative stress-induced hepatic injury by
            intracellular type II platelet-activating factor acetylhydrolase by
            metabolism of oxidized phospholipids in vivo
  JOURNAL   J Biol Chem 283 (3), 1628-1636 (2008)
   PUBMED   18024956
  REMARK    GeneRIF: These results indicate that PAF-AH (II) is involved in the
            metabolism of esterified 8-isoprostaglandin F(2alpha) and protects
            tissue from oxidative stress-induced injury.
REFERENCE   5  (bases 1 to 3136)
  AUTHORS   Prows,D.R., Hafertepen,A.P., Winterberg,A.V., Gibbons,W.J. Jr.,
            Liu,C. and Nick,T.G.
  TITLE     Genetic analysis of hyperoxic acute lung injury survival in
            reciprocal intercross mice
  JOURNAL   Physiol Genomics 30 (3), 271-281 (2007)
   PUBMED   17488888
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669982.10, BY233826.1, BQ179290.1, AK075918.1 and AI846306.1.
            
            Transcript Variant: This variant (3) uses an alternate 5' exon and
            it thus differs in its 5' UTR and initiates translation at a
            downstream in-frame start codon, compared to variant 1. The encoded
            isoform (b) is shorter at the N-terminus, compared to isoform a.
            Both variants 2 and 3 encode isoform b.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156774.1, BC025938.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AL669982.10        62216-62222
            8-363               BY233826.1         2-357
            364-944             BQ179290.1         133-713
            945-1010            AL669982.10        79262-79327
            1011-2083           AK075918.1         1-1073
            2084-2084           AL669982.10        85679-85679
            2085-3118           AK075918.1         1074-2107
            3119-3136           AI846306.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 66.68 cM"
     gene            1..3136
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /note="platelet-activating factor acetylhydrolase 2"
                     /db_xref="GeneID:100163"
                     /db_xref="MGI:MGI:2140321"
     exon            1..193
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..1276
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /EC_number="3.1.1.47"
                     /EC_number="2.3.1.149"
                     /note="isoform b is encoded by transcript variant 3;
                     platelet-activating factor acetylhydrolase 2, cytoplasmic;
                     SD-PLA2; PAF:sphingosine transacetylase;
                     PAF:lysophospholipid transacetylase; platelet-activating
                     factor acetyltransferase PAFAH2; serine-dependent
                     phospholipase A2"
                     /codon_start=1
                     /product="platelet-activating factor acetylhydrolase 2,
                     cytoplasmic isoform b"
                     /protein_id="NP_001272803.1"
                     /db_xref="CCDS:CCDS18771.1"
                     /db_xref="GeneID:100163"
                     /db_xref="MGI:MGI:2140321"
                     /translation="MGAGQSVCFPPISGPHHIGCTDVMEGHSLEGSLFRLFYPCQASE
                     KCEQPLWIPRYEYSMGLADYLQYNKRWVGLLFNVGIGSCRLPVSWNGPFKAKESGYPL
                     IILSHGLGGFRASYSAFCMELASRGFVVAAVEHRDQSAAATYFCKPTSQESSPAESLE
                     EEWLPFRRIKEGEKEFHVRNPQVHQRVKECVRVLRILQDASAGKTVVNVFPGGLDLMT
                     LKGSIDRNRVAVMGHSFGGATAVLALTQEVQFRCAIALDAWMFPLERDFYPKARGPVF
                     FINVEKFQTVESVNLMKKICAQHEQSRIVTVLGAVHRSQTDFAFVTGNLIAKFFSSNS
                     RGTLDPYESQEVMVRAILAFLQKHLDLKEDYDQWSSFIEGVGPSLIQGAPHYLSSL"
     exon            194..344
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            345..441
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            442..510
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            511..649
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            650..763
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            764..855
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1026
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1181
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..3121
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3096..3101
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3114
                     /gene="Pafah2"
                     /gene_synonym="2310074E22Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagaaacagc aagtgaggga gagaccagca agaggctgtt ctgtgttctg tcttccagcc
       61 agcaaattcc cgagcacccg gctttggtcc agctggtgat gagatggggg ccggccagtc
      121 tgtgtgcttc ccacccatct cgggacccca ccacattggc tgcacggatg tgatggaggg
      181 tcacagtctt gaggggagcc tcttccgact cttctaccct tgccaagcat cagagaagtg
      241 cgagcagccc ctgtggattc cccgctacga gtacagcatg ggtctggccg attacctgca
      301 gtataacaag cgctgggtgg ggttgctgtt caacgtgggt atcggatctt gtcgcttgcc
      361 tgttagctgg aacggcccct ttaaggcaaa ggagtctgga taccctttga tcatcttgtc
      421 ccatggtcta ggagggttca gggcgtcata ctcggccttc tgcatggagc tggcctctcg
      481 tggctttgtg gttgctgccg tagagcacag ggaccagtca gctgcggcca cctacttctg
      541 caagccgacc tcccaggaga gctctcccgc tgaatctctg gaggaggagt ggctcccctt
      601 ccgtcgcatc aaggaagggg agaaggagtt ccacgtcagg aatcctcagg tgcaccagcg
      661 ggtgaaagag tgtgtgcgag tgctgcggat ccttcaggac gcctctgctg ggaagactgt
      721 cgtcaatgtc ttcccaggag gactggatct gatgaccttg aagggcagca tcgacaggaa
      781 ccgcgtagcc gtgatgggtc actcctttgg aggagctacg gctgttcttg ctttgaccca
      841 ggaggtccag tttcggtgtg ctatagccct ggacgcttgg atgttccctc tggaacggga
      901 cttttacccc aaagcccggg gccctgtgtt ctttatcaac gtcgagaaat tccagacggt
      961 agagagcgtc aacttgatga agaagatctg tgcccagcac gagcagtcca ggatcgtgac
     1021 tgtcctaggc gctgttcatc ggagtcaaac tgactttgcc tttgtgacgg gcaacctgat
     1081 tgctaaattc ttttccagta actctcgtgg taccttggac ccttacgaaa gtcaggaggt
     1141 catggtgagg gccatcttgg ccttcctgca gaagcacctt gacctgaagg aagactacga
     1201 tcaatggagc agcttcatcg aaggcgtcgg accatcgctc atccaagggg ccccgcacta
     1261 tctgtctagt ctataggtcg cttgagctgc ccaaggatac ttgcagccaa tcagcaggac
     1321 cctttttccc agatggagaa gatgtaatca gagagctgat tgggtaactg gatactttgc
     1381 tcttggggtt gttgatctta aacgcacctc agatcactgg ctgattggga agcggatttt
     1441 tttcttttta agtcaaggac tcatgatcca gactggctct gaacttctta tgtattgagg
     1501 atgacccttc tgaatgacag acattaccac tatacttggc ttgtattctg tgctgggaat
     1561 cggacctgat atcagccact gtgcctgagt taaagactat gaagtactta tgtgctgagg
     1621 acctgttctc aatgacagtg gtccccacct ctgtcccagg tcctgtttgc cttaagtaaa
     1681 atagtgtcta cctgctgggc tgagttctct ctcttggctc aggccatttc tacggttctg
     1741 gccatgcagt ttaccatatg gaatccatac cagtaggtgc cattccgtct gtgccatcat
     1801 aacctcgtag ccggtctttt cggccccagg cagagcccca tggagcctgg atatgacaac
     1861 ttgacttggc tagcccactc tctcaccttg ggcaattgtt gcctcatgaa gccaaaggcc
     1921 agagggaaac cttagggctt ccagccctgc ctggcgctct ccacctgccc cctctctctc
     1981 cagagcagtt ctcctaccat atgaagtaca tcttcagggc agtcttccca tcacatgaaa
     2041 tacagtaatg gacactttgg accgtcatgc ttaggagccc atgtggggca aacagtattg
     2101 tcctcggaac acagacgcca aggagcccct cagaagttca gtgtctttga ggtttcgcaa
     2161 gtatgtcttg tcaggaagca tcctgagtag gttgagatgg gcaggaaact attaacagag
     2221 ctgagctgcc ctacatgggc cacagaggac accgggttgg ccagctcagc tgggggaatg
     2281 tccctagaga ggtctgaggt gagcttcgtc ttgcctctta ctcaaaccag ggagctttag
     2341 ttgtctgctt ttgtcttgtt tctggttttg gtttgttttt ttgaggccat ccctcaattt
     2401 aagtcaagga accgatccag tctgcttcct cctggttcgt tgaatattga tgctcatgtt
     2461 ggcctaacgg catgctggag ttccgcctgt ttaattgaca ttagagtgag ttggagagcc
     2521 atctggaagt gacttttccg atttcctttg tttgctaatc acgtgtgccc acacggaaaa
     2581 aaacaaaaaa caaaaaaacg aagacttccc agagttccaa gctctgtggg aagccccccc
     2641 caggactgtg tcgctgccat ataagatctg tcacctgaat ccaaggaggg aagaacctca
     2701 gaggagcaag tacagatcaa aagtgtttct ccgcacagta ttccaaggtc agggccactg
     2761 aattgatcca gagaggtgtg cacacatcag gaggcccctc aggtatggct ctgcagcccc
     2821 accgctgggc ttggctccaa cgctgcataa atcgcataaa tcggagcaaa tcctgccatc
     2881 ttcccaacct caattcctgc atatgtaaaa tgagggcctg cgctagcaac ggatggaatg
     2941 attgagtgga gggttgggtg acccctgggt tattaaatgt gcatttccca cgtcccacac
     3001 acatgctttc taatcgtttg aatattaaat gtttccaaga tagaatgttc catatgatct
     3061 ctacccttca tgattgttgg cccactcact gtatgattaa aaagtctata tccactctga
     3121 aaaaaaaaaa aaaaaa
//
