LOCUS       NM_001289678            1864 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus protein kinase C and casein kinase substrate in
            neurons 3 (Pacsin3), transcript variant 3, mRNA.
ACCESSION   NM_001289678 XM_006500422
VERSION     NM_001289678.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1864)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1864)
  AUTHORS   Seemann,E., Sun,M., Krueger,S., Troger,J., Hou,W., Haag,N.,
            Schuler,S., Westermann,M., Huebner,C.A., Romeike,B., Kessels,M.M.
            and Qualmann,B.
  TITLE     Deciphering caveolar functions by syndapin III KO-mediated
            impairment of caveolar invagination
  JOURNAL   Elife 6, e29854 (2017)
   PUBMED   29202928
  REMARK    GeneRIF: The authors show that muscle cells of syndapin III
            knockout mice show severe reductions of caveolae reminiscent of
            human caveolinopathies. The membrane-shaping protein syndapin III
            is crucial for caveolar invagination and gene knockout rendered the
            cells sensitive to membrane tensions.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1864)
  AUTHORS   Samasilp,P., Lopin,K., Chan,S.A., Ramachandran,R. and Smith,C.
  TITLE     Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine
            chromaffin cells
  JOURNAL   Am J Physiol Cell Physiol 306 (9), C831-C843 (2014)
   PUBMED   24500282
  REMARK    GeneRIF: Show that in neuroendocrine chromaffin cells fusion pore
            expansion and catecholamine release are limited specifically by
            mutation of syndapin 3.
REFERENCE   6  (bases 1 to 1864)
  AUTHORS   Cuajungco,M.P., Grimm,C., Oshima,K., D'hoedt,D., Nilius,B.,
            Mensenkamp,A.R., Bindels,R.J., Plomann,M. and Heller,S.
  TITLE     PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the
            subcellular localization of TRPV4
  JOURNAL   J Biol Chem 281 (27), 18753-18762 (2006)
   PUBMED   16627472
  REMARK    GeneRIF: PACSIN 3 specifically affects the endocytosis of TRPV4,
            thereby modulating the subcellular localization
REFERENCE   7  (bases 1 to 1864)
  AUTHORS   Desai,J., Shannon,M.E., Johnson,M.D., Ruff,D.W., Hughes,L.A.,
            Kerley,M.K., Carpenter,D.A., Johnson,D.K., Rinchik,E.M. and
            Culiat,C.T.
  TITLE     Nell1-deficient mice have reduced expression of extracellular
            matrix proteins causing cranial and vertebral defects
  JOURNAL   Hum Mol Genet 15 (8), 1329-1341 (2006)
   PUBMED   16537572
REFERENCE   8  (bases 1 to 1864)
  AUTHORS   Sumoy,L., Pluvinet,R., Andreu,N., Estivill,X. and Escarceller,M.
  TITLE     PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the
            pacsin-syndapin-FAP52 gene family
  JOURNAL   Gene 262 (1-2), 199-205 (2001)
   PUBMED   11179684
REFERENCE   9  (bases 1 to 1864)
  AUTHORS   Modregger,J., Ritter,B., Witter,B., Paulsson,M. and Plomann,M.
  TITLE     All three PACSIN isoforms bind to endocytic proteins and inhibit
            endocytosis
  JOURNAL   J Cell Sci 113 Pt 24, 4511-4521 (2000)
   PUBMED   11082044
REFERENCE   10 (bases 1 to 1864)
  AUTHORS   Howard,L., Nelson,K.K., Maciewicz,R.A. and Blobel,C.P.
  TITLE     Interaction of the metalloprotease disintegrins MDC9 and MDC15 with
            two SH3 domain-containing proteins, endophilin I and SH3PX1
  JOURNAL   J Biol Chem 274 (44), 31693-31699 (1999)
   PUBMED   10531379
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY250979.1, AF149824.1, AK049166.1 and AV333674.1.
            
            On Jan 25, 2014 this sequence version replaced XM_006500422.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. Variants 1 through 4 encode the same
            protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003884.1, AF149824.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                BY250979.1         2-96
            96-1292             AF149824.1         10-1206
            1293-1862           AK049166.1         1424-1993
            1863-1864           AV333674.1         255-256
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 50.61 cM"
     gene            1..1864
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /note="protein kinase C and casein kinase substrate in
                     neurons 3"
                     /db_xref="GeneID:80708"
                     /db_xref="MGI:MGI:1891410"
     exon            1..114
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            115..178
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            179..269
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..1490
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /codon_start=1
                     /product="protein kinase C and casein kinase II substrate
                     protein 3"
                     /protein_id="NP_001276607.1"
                     /db_xref="CCDS:CCDS16429.1"
                     /db_xref="GeneID:80708"
                     /db_xref="MGI:MGI:1891410"
                     /translation="MAPEEDAGGEVLGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQ
                     ERARIEKAYAQQLADWARKWRGAVEKGPQYGTLEKAWHAFFTAAERLSELHLEVREKL
                     HGPDSERVRTWQRGAFHRPVLGGFRESRAAEDGFRKAQKPWLKRLKEVEASKKSYHTA
                     RKDEKTAQTRESHAKADSSMSQEQLRKLQERVGRCTKEAEKMKTQYEQTLAELNRYTP
                     RYMEDMEQAFESCQAAERQRLLFFKDVLLTLHQHLDLSSSDKFHELHRDLQQSIEAAS
                     DEEDLRWWRSTHGPGMAMNWPQFEEWSLDTQRAISRKEKGGRSPDEVTLTSIVPTRDG
                     TAPPPQSPSSPGSGQDEDWSDEESPRKVATGVRVRALYDYAGQEADELSFRAGEELLK
                     MSEEDEQGWCQGQLQSGRIGLYPANYVECVGA"
     exon            270..426
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            427..662
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            663..818
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            819..994
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1115
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1252
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1374
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1864
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1826..1831
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /note="hexamer: ATTAAA"
     regulatory      1842..1847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1854
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
                     /note="major polyA site"
     polyA_site      1863
                     /gene="Pacsin3"
                     /gene_synonym="4921507A02Rik; 6330413E15Rik"
ORIGIN      
        1 gggggagggc ccgaagcgct ccaggaggcg gtggcccgga cccgggagtg gtaggactcc
       61 gaactgagcg tggccagatc cccctaaccc accgagggcc cctgagagga cacgacaggc
      121 tcatagtggc agcttggaga cttggacacg ggtcctggaa gtgatctgga gaccccagca
      181 ccacatctgc caccattccg tgctgcaggg ataccatggc tccagaggag gacgccggag
      241 gggaggtcct agggggcagt ttctgggagg ctggcaacta cagacggacg gtgcagaggg
      301 ttgaggatgg gcacaggctg tgtggggacc tggttagctg cttccaagaa cgtgcccgca
      361 ttgagaaggc ctacgcccag cagctggctg actgggcccg caagtggagg ggtgctgtgg
      421 agaagggccc acagtacggc accctggaga aggcctggca tgccttcttc actgcggctg
      481 agaggctgag cgagctgcac ttggaggtga gggagaagtt gcacggtcca gacagcgagc
      541 gagtgcggac ctggcagcga ggggctttcc accggccagt gctggggggc tttcgggaaa
      601 gccgggctgc agaggatggt ttccgtaaag ctcagaagcc ctggctaaag aggctgaagg
      661 aggttgaggc ttctaagaag agctaccaca cagcccgcaa ggatgagaag acagcccaga
      721 cccgggagag ccacgcgaag gcagacagct ccatgtccca ggagcagctt cgaaaattac
      781 aggagcgggt gggccgctgc accaaggagg cagagaagat gaaaacccag tatgagcaga
      841 ccctggccga gctaaatcgc tacaccccac gctacatgga ggacatggag caggcttttg
      901 agagctgcca ggctgctgag cgccagcggc ttctcttctt caaggatgtc ttgctcaccc
      961 tgcatcaaca cctcgacctc tccagtagcg acaagttcca tgaactccat cgagacctgc
     1021 agcagagcat tgaggctgcc agtgatgaag aggatctgcg ctggtggcgg agcacacacg
     1081 ggcctggcat ggccatgaac tggccacagt ttgaggaatg gtcgttagac acacagagag
     1141 ccatcagccg caaggagaag ggtggccgga gcccagatga ggttactctg accagcattg
     1201 taccgacaag agatggcacc gcacccccac cccagtcccc atcgtccccg ggtagcgggc
     1261 aggacgagga ctggtcagat gaggagagtc cccggaaagt tgccactggg gtgagggtac
     1321 gggcgcttta tgactatgct ggccaggagg ctgatgagct gagcttccga gcaggggaag
     1381 agttgctgaa gatgagcgag gaggatgagc agggctggtg ccagggccag ctgcagagtg
     1441 gccgcattgg tctgtaccct gccaattacg tagaatgtgt gggcgcctga gtgccctgac
     1501 agccctacca aggcgtctct ctgacctggg ccagagctta acccttcctg gactgctgga
     1561 cctgaaggcc ctgaaccatg tgctgctgct gtctctcttt tcccacagga ggaagtctca
     1621 gggcccatga agggcagggg tctgtgtcta gagttggagg ttggaatgat ggagggtcga
     1681 gaggtgcccg agcctcctcg ggaacatttg ggtcatccca gagtgggagg atccagctcc
     1741 taaactgttt aggagttcct ggggtgaggt tggggagaat gtagtttgag gctggcagca
     1801 ccttcttttg tgacttgtag tgtgcattaa atttgattaa gaataaaaac cccatcaagt
     1861 gttg
//
