LOCUS       NM_028944               1801 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus prolyl 4-hydroxylase, transmembrane (endoplasmic
            reticulum) (P4htm), transcript variant 1, mRNA.
ACCESSION   NM_028944
VERSION     NM_028944.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Byts,N., Sharma,S., Laurila,J., Paudel,P., Miinalainen,I.,
            Ronkainen,V.P., Hinttala,R., Tornquist,K., Koivunen,P. and
            Myllyharju,J.
  TITLE     Transmembrane Prolyl 4-Hydroxylase is a Novel Regulator of Calcium
            Signaling in Astrocytes
  JOURNAL   eNeuro 8 (1) (2021)
   PUBMED   33298456
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Leinonen,H., Koivisto,H., Lipponen,H.R., Matilainen,A., Salo,A.M.,
            Dimova,E.Y., Hamalainen,E., Staven,S., Miettinen,P., Myllyharju,J.,
            Koivunen,P. and Tanila,H.
  TITLE     Null mutation in P4h-tm leads to decreased fear and anxiety and
            increased social behavior in mice
  JOURNAL   Neuropharmacology 153, 63-72 (2019)
   PUBMED   31029587
  REMARK    GeneRIF: The knock-out of P4H-TM was linked to decreased fear and
            anxiety and increased social behavior.
REFERENCE   3  (bases 1 to 1801)
  AUTHORS   Leinonen,H., Rossi,M., Salo,A.M., Tiainen,P., Hyvarinen,J.,
            Pitkanen,M., Sormunen,R., Miinalainen,I., Zhang,C., Soininen,R.,
            Kivirikko,K.I., Koskelainen,A., Tanila,H., Myllyharju,J. and
            Koivunen,P.
  TITLE     Lack of P4H-TM in mice results in age-related retinal and renal
            alterations
  JOURNAL   Hum Mol Genet 25 (17), 3810-3823 (2016)
   PUBMED   27466183
  REMARK    GeneRIF: Results show that P4h-tm is expressed to a high degree in
            the mouse retinal pigment epithelium (RPE) and brain. P4h-tm-/-
            mice develop age-related renal dysfunction, resulting in
            proteinuria and impaired RPE functions, which eventually lead to an
            AMD-like pathology.
REFERENCE   4  (bases 1 to 1801)
  AUTHORS   Laitala,A., Aro,E., Walkinshaw,G., Maki,J.M., Rossi,M.,
            Heikkila,M., Savolainen,E.R., Arend,M., Kivirikko,K.I., Koivunen,P.
            and Myllyharju,J.
  TITLE     Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase
            regulating EPO production and erythropoiesis
  JOURNAL   Blood 120 (16), 3336-3344 (2012)
   PUBMED   22955912
REFERENCE   5  (bases 1 to 1801)
  AUTHORS   Hyvarinen,J., Hassinen,I.E., Sormunen,R., Maki,J.M.,
            Kivirikko,K.I., Koivunen,P. and Myllyharju,J.
  TITLE     Hearts of hypoxia-inducible factor prolyl 4-hydroxylase-2
            hypomorphic mice show protection against acute ischemia-reperfusion
            injury
  JOURNAL   J Biol Chem 285 (18), 13646-13657 (2010)
   PUBMED   20185832
  REMARK    GeneRIF: chronic stabilization of Hif-1alpha and Hif-2alpha by
            genetic knockdown of Hif-p4h-2 promotes cardioprotection by
            induction of many genes involved in glucose metabolism, cardiac
            function, and blood pressure.
REFERENCE   6  (bases 1 to 1801)
  AUTHORS   Li,L., Cai,X.J., Feng,M., Rong,Y.Y., Zhang,Y. and Zhang,M.
  TITLE     Effect of adiponectin overexpression on stability of preexisting
            plaques by inducing prolyl-4-hydroxylase expression
  JOURNAL   Circ J 74 (3), 552-559 (2010)
   PUBMED   20075561
  REMARK    GeneRIF: Adiponectin increases collagen production by inducing the
            expression of P4H, which may play a major role in the development
            of the thick fibrous cap of advanced atherosclerotic plaque
REFERENCE   7  (bases 1 to 1801)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046783.1 and BE944288.1.
            
            On Oct 28, 2017 this sequence version replaced NM_028944.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046783.1, AK047714.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1784              AK046783.1         1-1784
            1785-1801           BE944288.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.52 cM"
     gene            1..1801
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /note="prolyl 4-hydroxylase, transmembrane (endoplasmic
                     reticulum)"
                     /db_xref="GeneID:74443"
                     /db_xref="MGI:MGI:1921693"
     exon            1..405
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..1560
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /EC_number="1.14.11.29"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transmembrane prolyl 4-hydroxylase; HIF-prolyl hydroxylase
                     4; hypoxia-inducible factor prolyl hydroxylase 4"
                     /codon_start=1
                     /product="transmembrane prolyl 4-hydroxylase isoform 1"
                     /protein_id="NP_083220.3"
                     /db_xref="CCDS:CCDS23533.1"
                     /db_xref="GeneID:74443"
                     /db_xref="MGI:MGI:1921693"
                     /translation="MAAAVATVQRPEAETVEEASNLQWPLPPEHRPSGAATRPGDSED
                     APVRPLCKPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESTDPGPQRREQS
                     PQPVPTLGPLTRLEGIKVGYERKVQVVAGRDHFIRTLSLKPLLFEIPGFLSDEECRLI
                     IHLAQMKGLQRSQILPTEEYEEAMSAMQVSQLDLFQLLDQNHDGRLQLREVLAQTRLG
                     NGRWMTPENIQEMYSAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESNELVRNS
                     HHTWLHQGEGAHHVMRAIRQRVLRLTRLSPEIVEFSEPLQVVRYGEGGHYHAHVDSGP
                     VYPETICSHTKLVANESVPFETSCRYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLI
                     QDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGEVDDYSLHGGCLVTRG
                     TKWIANNWINVDPSRARQALFQQEMARLAREGGMDSQPEWALDRAYSDARVEL"
     exon            406..487
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     exon            488..678
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     exon            679..775
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     exon            776..938
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1124
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1215
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1339
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1786
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1746..1751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /note="hexamer: AATAAA"
     polyA_site      1786
                     /gene="P4htm"
                     /gene_synonym="4933406E20Rik; HIF-PH4; HPH-4; P4H-TM;
                     Ph-4"
                     /note="major polyA site"
ORIGIN      
        1 gagcaccccc gcggcccctc ccctgggcgc gcgcgcgacc tgggcgccat ggcggcagcg
       61 gtggcgaccg tgcagcggcc tgaggctgag acagtcgagg aggcctcaaa tcttcagtgg
      121 ccgctaccac cggagcatcg cccttccggg gcagcgacca gaccaggcga tagtgaagac
      181 gccccggtgc ggccactgtg caagccgcgg ggcatctgtt cccgtgccta cttcttggtg
      241 ctgatggtgt tcgtgcacct gtacttgggc aacgtgctgg ccctgctgct cttcgtgcac
      301 tacagcaacg gagacgagag cacagacccc ggaccccagc gccgcgagca gagcccccag
      361 ccagtgccaa ccttgggtcc cctcacccgg ctggagggca tcaaggtggg atatgagcgg
      421 aaagtccagg tggtcgcggg cagggatcac ttcatccgaa ccctcagcct caagccgctg
      481 ctttttgaaa tcccaggctt cctgagtgac gaggagtgtc ggctcatcat ccacctggca
      541 cagatgaaag ggttacagcg aagccagatc ctgcccactg aggagtatga ggaggcgatg
      601 agcgccatgc aggtcagcca gctggacctc ttccagctgt tggatcagaa ccacgacggt
      661 cgcctgcagc tccgagaggt cctggcccag actcgcctgg gaaatggacg gtggatgact
      721 ccagagaaca ttcaggagat gtactctgca atcaaagcag accctgacgg tgatggcgtg
      781 ctgagtctgc aggaattttc caacatggac ctccgagact tccacaagta catgaggagc
      841 cataaggcag aatccaatga gctggtgagg aacagccacc acacatggct ccaccaggga
      901 gagggagcac atcacgtcat gcgtgccatc cgccagaggg tcctgcgcct cactcgcctc
      961 tccccggaga ttgtggagtt cagtgagcca ctacaggttg tacgatatgg tgagggaggt
     1021 cactaccatg cccatgtgga cagcggaccc gtgtacccag agaccatctg ctcccatacc
     1081 aagctggtag ccaatgaatc tgtaccgttt gagacctcct gtcgctacat gacagtgctg
     1141 ttttatttga acaatgtcac cggtgggggt gagaccgtct tccctgtggc agacaacagg
     1201 acctatgatg aaatgagtct gattcaggat gatgtcgatc tccgggacac tcgaaggcac
     1261 tgtgacaagg gcaaccttcg tgtcaagccc cagcaaggaa ctgcagtctt ttggtacaac
     1321 taccttcctg atgggcaagg ctgggtgggt gaagtggacg actactcgtt gcatgggggc
     1381 tgcctggtca cccgcggcac caagtggatc gctaacaact ggatcaatgt ggaccctagc
     1441 cgggcacggc aagccttgtt ccagcaggag atggctcgtc tggctcggga aggtggcatg
     1501 gactcacaac ccgagtgggc cttggaccga gcctacagtg atgcccgtgt ggagctctga
     1561 cgcaaggccc tgatgcgaat cacccgctgc ccaacgtcaa agaccctgca ggtcctctgt
     1621 cctactgcag actgaagttc tggccgacct cttgccctag ccctccctgc ctgcagccgc
     1681 gatcaaaggc gcagttccta cattcacgtt atttattggt gtacagcctc catgctgctc
     1741 cgttaaataa aactacaggg cttccagcca ggggccggcg agctaaaaaa aaaaaaaaaa
     1801 a
//
