LOCUS       NM_011031               2284 bp    mRNA    linear   ROD 11-SEP-2024
DEFINITION  Mus musculus procollagen-proline, 2-oxoglutarate 4-dioxygenase
            (proline 4-hydroxylase), alpha II polypeptide (P4ha2), transcript
            variant 2, mRNA.
ACCESSION   NM_011031
VERSION     NM_011031.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Dang,X., Chen,X., Liang,Z., Dai,Z., Ding,W., Song,J. and Fu,J.
  TITLE     P4HA2 promotes tumor progression and is transcriptionally regulated
            by SP1 in colorectal cancer
  JOURNAL   Cancer Biol Ther 25 (1), 2361594 (2024)
   PUBMED   38857058
  REMARK    GeneRIF: P4HA2 promotes tumor progression and is transcriptionally
            regulated by SP1 in colorectal cancer.
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Salo,A.M., Rappu,P., Koski,M.K., Karjalainen,E., Izzi,V.,
            Drushinin,K., Miinalainen,I., Kapyla,J., Heino,J. and Myllyharju,J.
  TITLE     Collagen prolyl 4-hydroxylase isoenzymes I and II have sequence
            specificity towards different X-Pro-Gly triplets
  JOURNAL   Matrix Biol 125, 73-87 (2024)
   PUBMED   38081527
REFERENCE   3  (bases 1 to 2284)
  AUTHORS   Zhang,J., Lyu,Z., Li,B., You,Z., Cui,N., Li,Y., Li,Y., Huang,B.,
            Chen,R., Chen,Y., Peng,Y., Fang,J., Wang,Q., Miao,Q., Tang,R.,
            Gershwin,M.E., Lian,M., Xiao,X. and Ma,X.
  TITLE     P4HA2 induces hepatic ductular reaction and biliary fibrosis in
            chronic cholestatic liver diseases
  JOURNAL   Hepatology 78 (1), 10-25 (2023)
   PUBMED   36799463
  REMARK    GeneRIF: P4HA2 induces hepatic ductular reaction and biliary
            fibrosis in chronic cholestatic liver diseases.
REFERENCE   4  (bases 1 to 2284)
  AUTHORS   Shi,R., Gao,S., Smith,A.H., Li,H., Shao,M., Shangguan,J., Zhang,J.,
            Xu,J., Ye,J., Graham,L.M. and Li,C.
  TITLE     Superoxide-induced Type I collagen secretion depends on prolyl
            4-hydroxylases
  JOURNAL   Biochem Biophys Res Commun 529 (4), 1011-1017 (2020)
   PUBMED   32819558
  REMARK    GeneRIF: Superoxide-induced Type I collagen secretion depends on
            prolyl 4-hydroxylases.
REFERENCE   5  (bases 1 to 2284)
  AUTHORS   Funato,N. and Yanagisawa,H.
  TITLE     Deletion of the T-box transcription factor gene, Tbx1, in mice
            induces differential expression of genes associated with cleft
            palate in humans
  JOURNAL   Arch Oral Biol 95, 149-155 (2018)
   PUBMED   30121012
REFERENCE   6  (bases 1 to 2284)
  AUTHORS   Wenderfer,S.E., Slack,J.P., McCluskey,T.S. and Monaco,J.J.
  TITLE     Identification of 40 genes on a 1-Mb contig around the IL-4
            cytokine family gene cluster on mouse chromosome 11
  JOURNAL   Genomics 63 (3), 354-373 (2000)
   PUBMED   10704283
REFERENCE   7  (bases 1 to 2284)
  AUTHORS   Zhu,Y., Jong,M.C., Frazer,K.A., Gong,E., Krauss,R.M., Cheng,J.F.,
            Boffelli,D. and Rubin,E.M.
  TITLE     Genomic interval engineering of mice identifies a novel modulator
            of triglyceride production
  JOURNAL   Proc Natl Acad Sci U S A 97 (3), 1137-1142 (2000)
   PUBMED   10655497
REFERENCE   8  (bases 1 to 2284)
  AUTHORS   Annunen,P., Helaakoski,T., Myllyharju,J., Veijola,J.,
            Pihlajaniemi,T. and Kivirikko,K.I.
  TITLE     Cloning of the human prolyl 4-hydroxylase alpha subunit isoform
            alpha(II) and characterization of the type II enzyme tetramer. The
            alpha(I) and alpha(II) subunits do not form a mixed
            alpha(I)alpha(II)beta2 tetramer
  JOURNAL   J Biol Chem 272 (28), 17342-17348 (1997)
   PUBMED   9211872
REFERENCE   9  (bases 1 to 2284)
  AUTHORS   Frazer,K.A., Ueda,Y., Zhu,Y., Gifford,V.R., Garofalo,M.R.,
            Mohandas,N., Martin,C.H., Palazzolo,M.J., Cheng,J.F. and Rubin,E.M.
  TITLE     Computational and biological analysis of 680 kb of DNA sequence
            from the human 5q31 cytokine gene cluster region
  JOURNAL   Genome Res 7 (5), 495-512 (1997)
   PUBMED   9149945
REFERENCE   10 (bases 1 to 2284)
  AUTHORS   Helaakoski,T., Annunen,P., Vuori,K., MacNeil,I.A., Pihlajaniemi,T.
            and Kivirikko,K.I.
  TITLE     Cloning, baculovirus expression, and characterization of a second
            mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an
            alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta
            subunit
  JOURNAL   Proc Natl Acad Sci U S A 92 (10), 4427-4431 (1995)
   PUBMED   7753822
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL596103.22, AK145535.1, U16163.1, CD563665.1, AK142689.1 and
            AV252891.1.
            
            On Apr 16, 2009 this sequence version replaced NM_011031.1.
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1218685.1,
                                           SRR1660819.65487.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                AL596103.22        5343-5416
            75-118              AK145535.1         2-45
            119-1582            U16163.1           6-1469
            1583-1584           CD563665.1         441-442
            1585-1951           U16163.1           1472-1838
            1952-2272           AK142689.1         641-961
            2273-2284           AV252891.1         186-197
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 32.13 cM"
     gene            1..2284
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /note="procollagen-proline, 2-oxoglutarate 4-dioxygenase
                     (proline 4-hydroxylase), alpha II polypeptide"
                     /db_xref="GeneID:18452"
                     /db_xref="MGI:MGI:894286"
     exon            1..117
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            118..245
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            246..351
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     CDS             264..1877
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /EC_number="1.14.11.2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; prolyl 4-hydroxylase subunit alpha-2; Prolyl
                     4-hydroxylase alpha IIa subunit; 4-PH alpha-2;
                     procollagen-proline,2-oxoglutarate-4-dioxygenase subunit
                     alpha-2"
                     /codon_start=1
                     /product="prolyl 4-hydroxylase subunit alpha-2 isoform 2
                     precursor"
                     /protein_id="NP_035161.2"
                     /db_xref="CCDS:CCDS24691.1"
                     /db_xref="GeneID:18452"
                     /db_xref="MGI:MGI:894286"
                     /translation="MKLQVLVLVLLMSWFGVLSWVQAEFFTSIGHMTDLIYAEKDLVQ
                     SLKEYILVEEAKLAKIKSWASKMEALTSRSAADPEGYLAHPVNAYKLVKRLNTDWPAL
                     GDLVLQDASAGFVANLSVQRQFFPTDEDESGAARALMRLQDTYKLDPDTISRGELPGT
                     KYQAMLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSLVLDYLSYA
                     VFQLGDLHRAVELTRRLLSLDPSHERAGGNLRYFERLLEEERGKSLSNQTDAGLATQE
                     NLYERPTDYLPERDVYESLCRGEGVKLTPRRQKKLFCRYHHGNRVPQLLIAPFKEEDE
                     WDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEED
                     DDPVVARVNRRMQHITGLTVKTAELLQVANYGMGGQYEPHFDFSRSDEQDAFKRLGTG
                     NRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPV
                     LVGCKWVSNKWFHERGQEFLRPCGTTEVD"
     exon            352..448
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            449..600
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            601..738
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            739..978
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1172
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1349
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1420
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1520
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1574
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1640
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1709
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1806
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..2284
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2248..2253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /note="hexamer: AATAAA"
     polyA_site      2277
                     /gene="P4ha2"
                     /gene_synonym="P4hl"
                     /note="major polyA site"
ORIGIN      
        1 ggaggtgggg acgtggcgga ggctcaagcg ggcggagact gtgacgaggg cggagccgag
       61 agcgccgggg cgacgccgcg ggaggttctg gaaacgccgg cttctgcgcg tgttcagttt
      121 cagagaccgg tggcgattgg ctgactgatt caacaaatag agcattctct gtgcctggag
      181 actttcaagg actgaggcag gcagaaggga agactcagaa agttcaggtc cagagcatca
      241 gcaaggtact gccctttcca gttatgaagc tccaggtgtt ggtgttggtg ttgctgatgt
      301 cctggttcgg tgtcctgagc tgggtgcagg cagaattctt cacctccatt gggcacatga
      361 ccgatctgat ttacgcagag aaggacctgg tacagtctct gaaggagtac atccttgtgg
      421 aggaagccaa gctcgccaag attaagagct gggccagcaa gatggaagcc ctgaccagca
      481 gatcagctgc cgaccccgag ggctacctgg ctcatcctgt gaatgcctac aagctggtga
      541 agcggttgaa cacagactgg cctgcactgg gggaccttgt ccttcaggat gcttcggcag
      601 gttttgtcgc taacctctca gttcagcggc aattcttccc cactgatgag gacgagtctg
      661 gagctgccag agccctgatg agacttcagg acacgtacaa actggatccg gacacgattt
      721 ccagagggga acttccaggc acaaagtacc aggccatgct gagtgtggac gactgctttg
      781 ggctgggccg ctcagcttac aatgaaggag actattacca tactgtgctg tggatggagc
      841 aggtactgaa gcagctcgat gctggggagg aggccactgt taccaagtcc ctggtgctgg
      901 actacctgag ctatgctgtc ttccaactgg gtgacctgca ccgtgctgtg gaactcaccc
      961 gccgcctgct ctctcttgac ccaagccacg aacgagctgg agggaatctg cggtactttg
     1021 aacggttgtt agaggaagaa agagggaaat cactgtcaaa tcagacagac gccggactgg
     1081 ccacccagga aaacttgtac gagaggccca cggactacct gcctgagagg gatgtgtacg
     1141 agagcctgtg tcgaggggag ggcgtgaaac tgacaccccg gaggcagaag aagcttttct
     1201 gtaggtacca tcatggaaac agagtgccac agctcctcat cgcccccttc aaagaggaag
     1261 acgagtggga cagcccacac atcgtcaggt actatgatgt gatgtccgac gaagaaatcg
     1321 agaggatcaa ggagattgct aagcccaaac ttgcacgagc cactgtgcgt gaccccaaga
     1381 caggtgtcct cactgttgcc agctacagag tttccaaaag ctcctggcta gaggaggatg
     1441 acgaccctgt tgtggcccgg gtcaaccggc ggatgcaaca tatcaccggg ctaacggtga
     1501 agactgcaga gctattgcag gtcgcaaact acggaatggg gggacagtac gaaccacact
     1561 ttgacttctc aaggagcgat gagcaagatg ctttcaagcg tttagggact gggaaccgtg
     1621 tggccacgtt tctaaactac atgagcgatg tcgaagctgg tggtgccacc gtctttcctg
     1681 acttgggagc tgctatttgg cccaagaagg gcacagctgt attctggtac aaccttcttc
     1741 gcagtgggga aggtgattat cggacgagac atgcagcctg ccctgtgctt gtgggctgca
     1801 agtgggtctc caacaagtgg ttccatgagc gaggacagga gttcttaaga ccttgtggaa
     1861 caacggaagt tgattgacgt ccttttctct ccgctcctcc ctggcccata gtccaaatca
     1921 tcttcaagtt caacatgaca gcttcctttt tatgtcccag ctcctgtcag gcaggtcatt
     1981 ggaggagcca gtgtttgact gaattgagag agtatatcct gagcctagtc ctgggtgacc
     2041 tgggccccag actctgacca gcttacacct gccctggctc tgggggtgtc ttggcatggc
     2101 tgcggtagag ccagactata gcacccggca cggtgccttt gtacctcaga tatttcaggt
     2161 agaagatgtc tcagtgaaac caaagttctg atgctgttta catgtgtgtt tttatcacat
     2221 ttctatttgt tgtggcttta accaaaaaat aaaaatgttc ctgccagaag ccttaaatag
     2281 cctt
//
