LOCUS       NM_001316371            4057 bp    mRNA    linear   ROD 17-MAR-2024
DEFINITION  Mus musculus procollagen-proline, 2-oxoglutarate 4-dioxygenase
            (proline 4-hydroxylase), alpha 1 polypeptide (P4ha1), transcript
            variant 2, mRNA.
ACCESSION   NM_001316371 XM_006513340
VERSION     NM_001316371.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Salo,A.M., Rappu,P., Koski,M.K., Karjalainen,E., Izzi,V.,
            Drushinin,K., Miinalainen,I., Kapyla,J., Heino,J. and Myllyharju,J.
  TITLE     Collagen prolyl 4-hydroxylase isoenzymes I and II have sequence
            specificity towards different X-Pro-Gly triplets
  JOURNAL   Matrix Biol 125, 73-87 (2024)
   PUBMED   38081527
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Cao,J., Zhong,Q., Huang,Y., Zhu,M., Wang,Z. and Xiong,Z.
  TITLE     Identification and validation of INHBE and P4HA1 as hub genes in
            non-alcoholic fatty liver disease
  JOURNAL   Biochem Biophys Res Commun 686, 149180 (2023)
   PUBMED   37922570
  REMARK    GeneRIF: Identification and validation of INHBE and P4HA1 as hub
            genes in non-alcoholic fatty liver disease.
REFERENCE   3  (bases 1 to 4057)
  AUTHORS   Wang,N., Langfelder,P., Stricos,M., Ramanathan,L., Richman,J.B.,
            Vaca,R., Plascencia,M., Gu,X., Zhang,S., Tamai,T.K., Zhang,L.,
            Gao,F., Ouk,K., Lu,X., Ivanov,L.V., Vogt,T.F., Lu,Q.R.,
            Morton,A.J., Colwell,C.S., Aaronson,J.S., Rosinski,J., Horvath,S.
            and Yang,X.W.
  TITLE     Mapping brain gene coexpression in daytime transcriptomes unveils
            diurnal molecular networks and deciphers perturbation gene
            signatures
  JOURNAL   Neuron 110 (20), 3318-3338 (2022)
   PUBMED   36265442
REFERENCE   4  (bases 1 to 4057)
  AUTHORS   Shi,R., Gao,S., Smith,A.H., Li,H., Shao,M., Shangguan,J., Zhang,J.,
            Xu,J., Ye,J., Graham,L.M. and Li,C.
  TITLE     Superoxide-induced Type I collagen secretion depends on prolyl
            4-hydroxylases
  JOURNAL   Biochem Biophys Res Commun 529 (4), 1011-1017 (2020)
   PUBMED   32819558
  REMARK    GeneRIF: Superoxide-induced Type I collagen secretion depends on
            prolyl 4-hydroxylases.
REFERENCE   5  (bases 1 to 4057)
  AUTHORS   Yorgan,T.A., Peters,S., Amling,M. and Schinke,T.
  TITLE     Osteoblast-specific expression of Panx3 is dispensable for
            postnatal bone remodeling
  JOURNAL   Bone 127, 155-163 (2019)
   PUBMED   31202927
REFERENCE   6  (bases 1 to 4057)
  AUTHORS   Holster,T., Pakkanen,O., Soininen,R., Sormunen,R., Nokelainen,M.,
            Kivirikko,K.I. and Myllyharju,J.
  TITLE     Loss of assembly of the main basement membrane collagen, type IV,
            but not fibril-forming collagens and embryonic death in collagen
            prolyl 4-hydroxylase I null mice
  JOURNAL   J Biol Chem 282 (4), 2512-2519 (2007)
   PUBMED   17135260
REFERENCE   7  (bases 1 to 4057)
  AUTHORS   Wandzioch,E., Kolterud,A., Jacobsson,M., Friedman,S.L. and
            Carlsson,L.
  TITLE     Lhx2-/- mice develop liver fibrosis
  JOURNAL   Proc Natl Acad Sci U S A 101 (47), 16549-16554 (2004)
   PUBMED   15536133
REFERENCE   8  (bases 1 to 4057)
  AUTHORS   Rogers,M.S., Rohan,R.M., Birsner,A.E. and D'Amato,R.J.
  TITLE     Genetic loci that control vascular endothelial growth
            factor-induced angiogenesis
  JOURNAL   FASEB J 17 (14), 2112-2114 (2003)
   PUBMED   12958152
REFERENCE   9  (bases 1 to 4057)
  AUTHORS   Helaakoski,T., Annunen,P., Vuori,K., MacNeil,I.A., Pihlajaniemi,T.
            and Kivirikko,K.I.
  TITLE     Cloning, baculovirus expression, and characterization of a second
            mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an
            alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta
            subunit
  JOURNAL   Proc Natl Acad Sci U S A 92 (10), 4427-4431 (1995)
   PUBMED   7753822
REFERENCE   10 (bases 1 to 4057)
  AUTHORS   Helaakoski,T., Pajunen,L., Kivirikko,K.I. and Pihlajaniemi,T.
  TITLE     Increases in mRNA concentrations of the alpha and beta subunits of
            prolyl 4-hydroxylase accompany increased gene expression of type IV
            collagen during differentiation of mouse F9 cells
  JOURNAL   J Biol Chem 265 (20), 11413-11416 (1990)
   PUBMED   1694844
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BE985798.1, BY721363.1, AK045008.1, AK142352.1, AK149127.1 and
            BX518478.1.
            
            [WARNING] On Mar 18, 2024 this sequence was replaced by
            NM_001316371.2.
            
            On Oct 20, 2015 this sequence version replaced XM_006513340.2.
            
            Transcript Variant: This variant (2) contains an alternate in-frame
            exon in the central coding region, compared to variant 1. The
            encoded isoform (2) is longer than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045008.1, SRR1660815.244360.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BE985798.1         1-19
            20-178              BY721363.1         1-159
            179-2495            AK045008.1         108-2424
            2496-2990           AK045008.1         2426-2920
            2991-3096           AK142352.1         45-150
            3097-3295           AK142352.1         152-350
            3296-4038           AK149127.1         3187-3929
            4039-4057           BX518478.1         343-361
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 29.52 cM"
     gene            1..4057
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /note="procollagen-proline, 2-oxoglutarate 4-dioxygenase
                     (proline 4-hydroxylase), alpha 1 polypeptide"
                     /db_xref="GeneID:18451"
                     /db_xref="MGI:MGI:97463"
     exon            1..181
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            182..284
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..1813
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /EC_number="1.14.11.2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; prolyl 4-hydroxylase subunit alpha-1;
                     procollagen-proline,2-oxoglutarate-4-dioxygenase subunit
                     alpha-1; 4-PH alpha-1"
                     /codon_start=1
                     /product="prolyl 4-hydroxylase subunit alpha-1 isoform 2
                     precursor"
                     /protein_id="NP_001303300.1"
                     /db_xref="CCDS:CCDS83698.1"
                     /db_xref="GeneID:18451"
                     /db_xref="MGI:MGI:97463"
                     /translation="MIWVVLMMAILLPQSLAHPGFFTSIGQMTDLIHNEKDLVTSLKD
                     YIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLI
                     LKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVQHKS
                     FLTAEDCFELGKVAYTEADYYHTELWMEQALTQLEEGELSTVDKVSVLDYLSYAVYQQ
                     GDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTAPKKK
                     GIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDK
                     PRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDP
                     VVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRI
                     ATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVG
                     NKWVSNKWLHERGQEFRRPCTLSELE"
     exon            285..381
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            382..533
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            534..671
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            672..911
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1108
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1285
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1356
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1456
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1510
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1576
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1645
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1742
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..4043
                     /gene="P4ha1"
                     /gene_synonym="P4ha"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcccc ctctccggcg cgccggcgcg gcaggggaat ataaaaggga cgcctggctc
       61 cctctactgc ccagtcgcac tgccagcagg ctgagggcgg cagggagcta gctagcgggg
      121 actgcaggcg acaggactga cccgagccga gctgtcgcct tcctcccggc ccgctatcca
      181 ggtgtgtgaa cctgtgaagt tccccaagat gatctgggtt gttttaatga tggcgattct
      241 acttcctcag tctttagccc atccagggtt ttttacgtca attggtcaga tgactgactt
      301 gatccataat gagaaagacc tggtgacatc actaaaagat tatattaaag cagaagagga
      361 caagttagag caaatcaaaa aatgggcaga gaagttagac cggctaacga gtacagcaac
      421 aaaagaccca gaggggtttg ttgggcatcc tgtaaacgca ttcaagttaa tgaaacgtct
      481 gaacactgag tggagtgagt tggagaatct gatcctcaag gacatgtcgg atggcttcat
      541 ctctaaccta accattcaga ggcagtactt ccctaatgat gaagatcagg ttggagctgc
      601 taaagctctg tttcggctgc aagacaccta caatctggac acaaatacca tctcaaaggg
      661 gaatcttcca ggagtgcaac ataagtcttt cctgacagcc gaggactgct ttgagctggg
      721 caaagtggcc tatacagagg cagattatta ccacacagaa ctgtggatgg agcaggctct
      781 gacgcagctg gaggagggag agctgtctac tgtagataaa gtctctgttc tggattacct
      841 gagctatgcg gtgtaccagc agggagacct ggataaggcg cttctcctca ccaagaagct
      901 tcttgagcta gatcctgaac atcagagagc taatggtaac ttagtatatt ttgaatatat
      961 aatgagtaaa gaaaaagatg ccaataagtc agcgtcgggc gaccaatctg atcagaaaac
     1021 tgcaccaaag aaaaaaggga tagctgtgga ttacctgcca gagagacaga agtacgaaat
     1081 gctgtgccgt ggggagggta tcaaaatgac tccccggaga cagaaaagac tgttctgccg
     1141 ctaccatgat gggaaccgga atcctaagtt catcctggcc ccagccaagc aggaggacga
     1201 gtgggacaag ccccggatta tccgtttcca tgacatcatc tctgacgcgg agattgagat
     1261 tgtcaaagac ttagcaaaac caaggctgag ccgagctaca gtacatgacc ctgagacggg
     1321 gaaattgacc acagcacagt acagagtatc taagagtgct tggctgtctg gctacgaaga
     1381 ccctgtggta tcccgaatta atatgagaat acaagatctc acaggactgg atgtttctac
     1441 ggccgaggag ttacaggtag caaattacgg agttggagga cagtatgagc cccattttga
     1501 ctttgcaagg aaagatgagc cggatgcctt tagagagctt gggacaggaa ataggattgc
     1561 cacgtggctg ttctatatga gcgatgtgtc tgctggagga gctactgttt ttcctgaagt
     1621 gggagccagt gtttggccca aaaaaggcac tgctgtcttc tggtataatc tgtttgccag
     1681 tggggaagga gattacagta cacggcatgc agcctgtccc gtgctagttg gaaacaaatg
     1741 ggtatccaac aaatggctcc atgaacgtgg acaagaattt cgaaggccgt gcaccctgtc
     1801 agaattggaa tgacaaccag gcttcccttg gctcctgttg tcctctaacg caccaggcac
     1861 gatggctgat tataactccg acgtttacag ctgactaaca ctccatgatt aattgggcca
     1921 tgagcctcag ccccatgtct cacctgtgga caatcactta ttttgtggat ttctttaatt
     1981 aatactcaat caccacatga tgtaaaactt atggttcagt tttcacagga tacaaggcat
     2041 tgaaaatgag gaacctaatt ttgttgttta aaagagcttt ttggttaatt tttttaaaaa
     2101 aagcatccag aagtagattt caccacaagt caggagctgg tggagcaggc gaggctgtgt
     2161 ggcaggcgtg caggtggcag gcgagcagag cctgtgggct tacagacggc aggcatgcag
     2221 acgccagtgt gctgctcttc cgatgtgtgg actgctgagc actggtaact aactagggcc
     2281 cagtcaggtc tgctattcca cgtgcagagt tcctattatt taaagaaact ctttttatca
     2341 aatccccctc ctcaccacgg gtgaattcag ccagtttgag aaaattactt tgttcaaaaa
     2401 gtcctgcaga actggcctcc caaccgagtg ctcagccttc gcccttttcc tcagtgactg
     2461 caggttgtcc agagactgtt ccaagtgcca atcagctgca gcaggcactg gtgttggcat
     2521 tctgattcaa ttctgtgcct taggagactg ggagttagaa aacggacagg tccttggact
     2581 gagggagttg atttttaaaa agaaatgttt tataagaagc caagatgttt gtttttctcc
     2641 attctgaaat gtgttataac aatgacctat ttatgatctt aaatcttttt tttttaaaaa
     2701 gtctctgtgt ggtattttca tgtttaaatc caaatgtaag aattgagcac tgctcccagg
     2761 cccgttctaa tttacttctt tactgcacag agcttagatt tactactcac ggttagacct
     2821 tcgtcaccca gaacaggctc ccttgggata agcttcgggg aatttgttcc aggatggtga
     2881 ggctttggtg tgtgttgtta cagtgaagac cctgcagttc ctcagaactg cagttcagaa
     2941 gcaggggcgt ggtctcggcc ctgcacttga tgaatatgaa ggaattcttt acacggggct
     3001 ccggctcatt ttaattctcc aagaaagtag atattaaaat gagctgtgtc catcacaagg
     3061 attttagcaa aaaaaaaaaa aaaaaaaaaa aaaaagtcaa agattaaggt cagcaattta
     3121 agctgattgt gtaaccaaac tgttttaaaa aacaacaggc tggaaatggt aggcccatat
     3181 ctttggtcct agcacttagg aggcagcagc agcagaatct ctctgagttc aaagacagcc
     3241 tggcctacat agtgagttct gggtcagtca gggctacata gaaagatccc atctcaaaaa
     3301 caggcaaaag aaattctttt aggctagttt tatagcttag tcagagcacc agtgtgagtc
     3361 cctgggttcc attcccacta ccaaagccct gtattctagc taacatgaaa gcaacagatt
     3421 ttacaagtaa aatagggatt tcaaggctaa aaaacagttc tgtcacaagg tgttagcaag
     3481 cagcttgtaa gttaggaagc taacatatgc atcagcctgc ctatatcctg agtactgatg
     3541 agaaagtggg gactttgttg aaaccagcaa tggcctataa acacgtgagg aaacactcca
     3601 tctctgggcc tttaagaaga tcagcagtgt taacccaggg tcctatcagg acaataaagt
     3661 tgcctagaat gtgtgagatc tgttattatg gaaaagttcc tcaaaattca atttcctatc
     3721 atctgtttct ggatatgaaa tgaagacagg ggtggaatgt ttataccagt gctcataaca
     3781 ggattgtttg taatcaccat aaagaggaag caacctaagt tcagaaacag gtgagtggag
     3841 aaatgatggc atgtgcatta gaaaaatatc cacagccaat tcatcccact caacatggat
     3901 gaacccactt acaggagatc cctagaggag ccaatggtgc ctgagccgag atgagacact
     3961 tggagttagt atttaatggg tgtgttgcac agcaatgtaa tacatttact gtgatctaaa
     4021 tacactcttt ggtttcagat ttaaaaaaaa aaaaaaa
//
