LOCUS       NM_176830               2047 bp    mRNA    linear   ROD 04-AUG-2024
DEFINITION  Mus musculus prolyl 3-hydroxylase family member 4 (non-enzymatic)
            (P3h4), transcript variant 2, mRNA.
ACCESSION   NM_176830 XM_982688
VERSION     NM_176830.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Hudson,D.M., Weis,M., Rai,J., Joeng,K.S., Dimori,M., Lee,B.H.,
            Morello,R. and Eyre,D.R.
  TITLE     P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine
            Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos
            Syndrome Type VIA
  JOURNAL   J Biol Chem 292 (9), 3877-3887 (2017)
   PUBMED   28115524
  REMARK    GeneRIF: both P3h3- and Sc65-null mice displayed a similar pattern
            of severe under-hydroxylation of lysines, particularly at
            cross-linking sites in type I collagen from bone, skin, dentin,
            cornea, and aorta, revealing a complex evolution and interplay
            between the proteins forming the machinery of collagen prolyl
            3-hydroxylation and lysyl hydroxylation in the ER
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Klank,R.L., Decker Grunke,S.A., Bangasser,B.L., Forster,C.L.,
            Price,M.A., Odde,T.J., SantaCruz,K.S., Rosenfeld,S.S., Canoll,P.,
            Turley,E.A., McCarthy,J.B., Ohlfest,J.R. and Odde,D.J.
  TITLE     Biphasic Dependence of Glioma Survival and Cell Migration on CD44
            Expression Level
  JOURNAL   Cell Rep 18 (1), 23-31 (2017)
   PUBMED   28052252
  REMARK    Erratum:[Cell Rep. 2017 Apr 18;19(3):668. doi:
            10.1016/j.celrep.2017.03.074. PMID: 28423327]
REFERENCE   3  (bases 1 to 2047)
  AUTHORS   Heard,M.E., Besio,R., Weis,M., Rai,J., Hudson,D.M., Dimori,M.,
            Zimmerman,S.M., Kamykowski,J.A., Hogue,W.R., Swain,F.L.,
            Burdine,M.S., Mackintosh,S.G., Tackett,A.J., Suva,L.J., Eyre,D.R.
            and Morello,R.
  TITLE     Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum
            Complex Regulating Collagen Lysyl Hydroxylation
  JOURNAL   PLoS Genet 12 (4), e1006002 (2016)
   PUBMED   27119146
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2047)
  AUTHORS   Gruenwald,K., Castagnola,P., Besio,R., Dimori,M., Chen,Y.,
            Akel,N.S., Swain,F.L., Skinner,R.A., Eyre,D.R., Gaddy,D., Suva,L.J.
            and Morello,R.
  TITLE     Sc65 is a novel endoplasmic reticulum protein that regulates bone
            mass homeostasis
  JOURNAL   J Bone Miner Res 29 (3), 666-675 (2014)
   PUBMED   23959653
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL590968.8.
            
            On Mar 17, 2020 this sequence version replaced NM_176830.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029271.1, BC031856.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-540               AL590968.8         131153-131692       c
            541-693             AL590968.8         130903-131055       c
            694-865             AL590968.8         130585-130756       c
            866-994             AL590968.8         129677-129805       c
            995-1140            AL590968.8         128705-128850       c
            1141-1224           AL590968.8         128090-128173       c
            1225-1369           AL590968.8         126339-126483       c
            1370-2047           AL590968.8         125443-126120       c
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 63.47 cM"
     gene            1..2047
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /note="prolyl 3-hydroxylase family member 4
                     (non-enzymatic)"
                     /db_xref="GeneID:66180"
                     /db_xref="MGI:MGI:1913430"
     exon            1..540
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     exon            541..693
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     exon            694..865
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     exon            866..994
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1140
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1224
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1369
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..2047
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1745..1750
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /note="hexamer: AACAAA"
     polyA_site      1770
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /note="major polyA site"
     regulatory      2023..2028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
                     /note="hexamer: AATAAA"
     polyA_site      2047
                     /gene="P3h4"
                     /gene_synonym="1110036O03Rik; Leprel4; Sc65"
ORIGIN      
        1 gagctggctg gagcccgacg gtcgccggct gctccgtaag cctccgggcg cggtgcgggc
       61 atggctcgcg gggcgtgggg gctgctgtgg ctgctgctgg gcagcgccgg ggcccagtac
      121 gagaaataca gcttccgagg attccctccg gaggacctga tgccactggc cacggcctac
      181 ggccacgctc tggagcaata cgaaggcgaa agctggcgcg agagcgcgcg ctacctggag
      241 gcggcgctgc ggctgcaccg gctgctgcgc gacagcgagg ccttctgcca cgccaactgt
      301 agtggccccg ccacctctca gcctcggccc gcgtccggcc cggacggcga ccacgacggc
      361 gatggcgagg actgggctcg cgagctgcgg ctcttcggtc acgtcctgga gcgcgccgcc
      421 tgcctgcggc gctgcaagaa gacgctgccc gcctttcaag tcccttaccc accgcggcag
      481 ctgctgcgcg acttccagag ccgcctgccc tatcagtacc tgcactacgc gcacttcaag
      541 gcgaaccgac tggagaaggc ggtggctgcg gcctacacct tcctccagag gaaccccaag
      601 cacgagctga ccgccaagta tctcaactac tacaggggga tgctggatat tggagatgag
      661 tccctcaccg acctggaggc ccagccctac gaggctgtgt tcctccgggc tgtgaaactt
      721 tacaacagcg gggactttcg cagcagcaca gaagacatgg agcgggccct ggctgaatat
      781 atgacggtct ttgctcggtg tctagctggc tgtgaggggg cccacgagca ggtagacttc
      841 aaggacttct acccagcgat agcagatctc ttcgcagaat ccctgcagtg caaggtggac
      901 tgtgagacca atctcacccc caacgtaggt ggcttctttg tggacaagtt tgtggccacc
      961 atgtatcact acctgcagtt tgcctactac aaactgaatg atgtacgcca ggctgcccgc
     1021 agtgctgcta gctacatgct ctttgacccc aaggacagcg ttatgcagca gaacctggta
     1081 tattaccgct tccaccgggc tcgctggggc cttgaagacg aggactttca accccgggag
     1141 gaagctatgc tctaccacaa ccagacctct gagctccggg agttgctaga cttcacacac
     1201 atgtaccttc agtcagacga tgagatggaa ctggaggaga cagaatcact cccagagcct
     1261 gaagaacctc tatctgatgc tgagttcgaa ggcgagggcg actatgagga aggtctctat
     1321 gctgactggt ggcaagagcc agaagccaag ggtgacgagg ccgaggccga gccagagcct
     1381 gaactgacat aaggcggtga catggcgcac tcttcaagct caggacacct ggctgggggc
     1441 aagaactatt tattaaaact taaaatagcc aggtgtggtg gcacacaccc ttaaccctgg
     1501 cacccggggg aggcagaagc aggcagatct cgggaccagc ccagcctaca cagtgagatc
     1561 ctggccaggc caggctacat aatgagattt tgtttcaaaa caaaaacaac tgagtgtggc
     1621 atcacatgcc tttaattgca gcagctggga tgcaggggca ggtggattgc ggtgagtttc
     1681 aggctagcct gtagtgagtt ctaggccagt agaggctctg ttgtaagacc ctgtctcaaa
     1741 ataaaacaaa aggaaaaacc ttaaaaggga cacagttgac ctagtttcca ctcagcctcc
     1801 accttagctc tctctctctc tcttgtgtct ttgaaccgca gactggtagc catattgctg
     1861 gttgggaagt gagctcttgg gggtctctgt ctccctttct gctcctgcgg aagttttcag
     1921 cttccggctt gagtctcaca ggaccaaggc cagccaggtg gcggttgtgt ctgagctgca
     1981 gagtgggttg tgaagaacat tgggcttaga gctctgctgc tcaataaatg tttattgttg
     2041 aaatgaa
//
