LOCUS       NM_019783               3210 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus prolyl 3-hydroxylase 1 (P3h1), transcript variant 2,
            mRNA.
ACCESSION   NM_019783
VERSION     NM_019783.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Wu,J., Li,H., Han,L., Sun,T., Tian,Y. and Wang,X.
  TITLE     The spatiotemporal expression pattern of Syndecans in murine
            embryonic teeth
  JOURNAL   Gene Expr Patterns 36, 119109 (2020)
   PUBMED   32220631
REFERENCE   3  (bases 1 to 3210)
  AUTHORS   Fratzl-Zelman,N., Bachinger,H.P., Vranka,J.A., Roschger,P.,
            Klaushofer,K. and Rauch,F.
  TITLE     Bone matrix hypermineralization in prolyl-3 hydroxylase 1 deficient
            mice
  JOURNAL   Bone 85, 15-22 (2016)
   PUBMED   26808442
  REMARK    GeneRIF: P3H1 deficiency leads to decreased deposition of
            extracellular matrix by osteoblasts and increased incorporation of
            mineral into the matrix.
REFERENCE   4  (bases 1 to 3210)
  AUTHORS   Street,V.A., Kujawa,S.G., Manichaikul,A., Broman,K.W.,
            Kallman,J.C., Shilling,D.J., Iwata,A.J., Robinson,L.C.,
            Robbins,C.A., Li,J., Liberman,M.C. and Tempel,B.L.
  TITLE     Resistance to noise-induced hearing loss in 129S6 and MOLF mice:
            identification of independent, overlapping, and interacting
            chromosomal regions
  JOURNAL   J Assoc Res Otolaryngol 15 (5), 721-738 (2014)
   PUBMED   24952082
REFERENCE   5  (bases 1 to 3210)
  AUTHORS   Homan,E.P., Lietman,C., Grafe,I., Lennington,J., Morello,R.,
            Napierala,D., Jiang,M.M., Munivez,E.M., Dawson,B., Bertin,T.K.,
            Chen,Y., Lua,R., Lichtarge,O., Hicks,J., Weis,M.A., Eyre,D. and
            Lee,B.H.
  TITLE     Differential effects of collagen prolyl 3-hydroxylation on skeletal
            tissues
  JOURNAL   PLoS Genet 10 (1), e1004121 (2014)
   PUBMED   24465224
  REMARK    GeneRIF: we generated knock-in mice carrying a single amino acid
            substitution in the catalytic site of P3h1 (Lepre1(H662A) ). This
            substitution abolished P3h1 activity but retained ability to form a
            complex with Crtap and thus the collagen chaperone
            Erratum:[PLoS Genet. 2014 Jun;10(6):e1004473]
REFERENCE   6  (bases 1 to 3210)
  AUTHORS   Tiainen,P., Pasanen,A., Sormunen,R. and Myllyharju,J.
  TITLE     Characterization of recombinant human prolyl 3-hydroxylase
            isoenzyme 2, an enzyme modifying the basement membrane collagen IV
  JOURNAL   J Biol Chem 283 (28), 19432-19439 (2008)
   PUBMED   18487197
REFERENCE   7  (bases 1 to 3210)
  AUTHORS   Vranka,J.A., Sakai,L.Y. and Bachinger,H.P.
  TITLE     Prolyl 3-hydroxylase 1, enzyme characterization and identification
            of a novel family of enzymes
  JOURNAL   J Biol Chem 279 (22), 23615-23621 (2004)
   PUBMED   15044469
REFERENCE   8  (bases 1 to 3210)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 3210)
  AUTHORS   Kaul,S.C., Sugihara,T., Yoshida,A., Nomura,H. and Wadhwa,R.
  TITLE     Gros1, a potential growth suppressor on chromosome 1: its identity
            to basement membrane-associated proteoglycan, leprecan
  JOURNAL   Oncogene 19 (32), 3576-3583 (2000)
   PUBMED   10951563
REFERENCE   10 (bases 1 to 3210)
  AUTHORS   Wassenhove-McCarthy,D.J. and McCarthy,K.J.
  TITLE     Molecular characterization of a novel basement membrane-associated
            proteoglycan, leprecan
  JOURNAL   J Biol Chem 274 (35), 25004-25017 (1999)
   PUBMED   10455179
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK010578.1, AK030436.1 and CO040878.1.
            
            On Jun 16, 2006 this sequence version replaced NM_019783.1.
            
            Transcript Variant: This variant (2) uses alternate splice sites in
            the 3' coding region and differs in the 3' UTR, compared to variant
            1. It encodes isoform 2, which is shorter and has a distinct
            C-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030436.1, SRR1660819.181903.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2160              AK010578.1         3-2162
            2161-2589           AK030436.1         2159-2587
            2590-3210           CO040878.1         1-621               c
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 55.34 cM"
     gene            1..3210
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /note="prolyl 3-hydroxylase 1"
                     /db_xref="GeneID:56401"
                     /db_xref="MGI:MGI:1888921"
     exon            1..516
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..2262
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /EC_number="1.14.11.7"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; growth suppressor 1; leucine- and
                     proline-enriched proteoglycan 1; leprecan 1"
                     /codon_start=1
                     /product="prolyl 3-hydroxylase 1 isoform 2 precursor"
                     /protein_id="NP_062757.2"
                     /db_xref="CCDS:CCDS38859.1"
                     /db_xref="GeneID:56401"
                     /db_xref="MGI:MGI:1888921"
                     /translation="MAVSERRLLAAMLAVAAAAALRVAAESEPGWDVAAPDLLYAEGT
                     AAYSRGDWPGVVLNMERALRSRAALRALRLRCRTRCATELPWAPDLDLGPDPSLSQDP
                     GAAALHDLRFFGAVLRRAACLRRCLGPPSAHLLSEELDLEFNKRSPYNYLQVAYFKIN
                     KLEKAVAAAHTFFVGNPEHMEMRQNLDYYQTMSGVKEADFRDLEAKPHMHEFRLGVRL
                     YSEEKPQEAVPHLEAALQEYFVADEECRALCEGPYDYDGYNYLDYSADLFQAITDHYV
                     QVLNCKQNCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAIECAKTYLLFF
                     PNDEVMHQNLAYYTAMLGEEEASSISPRENAEEYRRRSLLEKELLFFAYDIFGIPFVD
                     PDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLTR
                     EGGPLLYEGISLTMNSKVLNGSQRVVMDGVISDDECQELQRLTNAAATSGDGYRGQTS
                     PHTPNEKFYGVTVLKALKLGQEGKVPLQSARMYYNVTEKVRRVMESYFRLDTPLYFSY
                     SHLVCRTAIEESQAERKDSSHPVHVDNCILNAEALMCIKEPPAYTFRDYSAILYLNGD
                     FDGGNFYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLD
                     PRHSERDRVQADDLVKMLFSPEEVDLPQEQPLPDQQGSPEPGEESLSDRGSLHKDEL"
     exon            517..669
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            670..859
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..991
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1131
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1221
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1274
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1396
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1524
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1620
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1771
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1889
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1965
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2106
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..3193
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2564..2569
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /note="hexamer: AATAAA"
     polyA_site      2591
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /note="major polyA site"
     regulatory      3174..3179
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /note="hexamer: AATAAA"
     polyA_site      3193
                     /gene="P3h1"
                     /gene_synonym="2410024C15Rik; Gros1; Lepre1"
                     /note="major polyA site"
ORIGIN      
        1 gtgccccagg cgagcagtcc cgttaagtgg aggagcaagg ccatggcggt gagcgaaagg
       61 aggctgctgg cagcgatgct agcggtcgcc gccgccgccg ccttacgggt tgcggcggag
      121 tctgagccgg gatgggacgt ggcagcccct gacctgcttt acgcagaggg gaccgcggcc
      181 tactcgcgcg gggactggcc cggggtggtc ctgaacatgg agcgggcgct gcgctcgcgg
      241 gcggccctgc gtgccctccg cctgcgctgc cgcacacgct gtgccaccga actgccgtgg
      301 gcaccggacc tggatctcgg tccggacccc agcctgagcc aggacccggg cgccgccgcc
      361 ctgcacgacc tgcgcttctt cggagccgtg ctgcgccgtg ccgcctgcct acgccgctgc
      421 ctcgggccgc cctctgccca cttgctgagt gaagaactgg acctggagtt caacaagcgg
      481 agcccgtaca actacctgca ggtcgcctat ttcaagataa acaagctgga gaaagctgtg
      541 gctgcggcac acaccttctt tgtgggcaat cctgagcaca tggagatgcg gcagaacctc
      601 gactattacc aaaccatgtc tggggtgaag gaggcagact tcagggatct cgaggccaag
      661 ccccatatgc atgagtttcg gctgggggta cgactctact cagaggagaa gccacaggaa
      721 gctgtgcccc acctggaggc ggcactgcaa gagtactttg tggccgatga ggaatgccgt
      781 gccctctgcg aagggcccta tgactacgac ggctacaact acctagacta cagcgctgac
      841 ctcttccagg ccatcacaga tcattacgtc caggtcctca actgtaagca gaactgtgtc
      901 acggagctgg cttcccaccc aagtagggaa aagccctttg aagacttcct cccttcacac
      961 tataattacc tacagtttgc ctactacaac attgggaact atacacaagc tattgaatgt
     1021 gccaagacct acctcctctt ctttcccaat gatgaggtga tgcaccagaa tctggcttat
     1081 tacacagcca tgcttggaga agaagaggcc agctccatca gccccaggga gaatgccgag
     1141 gaataccgac gtcgaagcct gttggagaaa gaactgcttt tcttcgctta tgacattttt
     1201 ggaattccct ttgtggatcc ggactcatgg actccagaag aggtgattcc caagagactg
     1261 caggagaaac agaagtcgga acgcgaaacc gcagtccgca tctcccagga gattggcaac
     1321 cttatgaagg agatcgagac cctcgtggag gagaagacca aggagtcctt ggatgtgagc
     1381 cgactgacgc gggaaggtgg tcccctgctg tatgaaggca tcagtctcac catgaattcc
     1441 aaagtcttga atggctccca gcgggtggtg atggatggtg tgatctctga tgatgagtgc
     1501 caggagctgc agagactgac caatgcggca gcaacttcgg gagatggcta ccgaggtcag
     1561 acctccccac acaccccaaa tgaaaagttc tatggtgtta ctgtcctcaa agctctcaag
     1621 ctcgggcagg aaggaaaagt tcctctgcag agtgcccgca tgtactacaa cgtgacagag
     1681 aaggtgcggc gcgtcatgga gtcctacttc cgcctggaca cgcccctcta tttctcttat
     1741 tcccacctcg tgtgccgcac tgcaatagaa gagtcacagg ctgagaggaa ggacagtagc
     1801 caccccgtcc acgtggataa ctgcatcctg aatgccgaag ccctcatgtg tatcaaggag
     1861 cccccagcct acacgttccg ggactacagc gccatccttt acctcaatgg cgacttcgat
     1921 ggaggaaact tttacttcac agaactagat gccaagactg tgacggcaga ggtgcagccc
     1981 cagtgtggaa gggctgtggg attctcttct ggcactgaga acccacatgg agtgaaggct
     2041 gtcaccaggg ggcagcgctg cgccatcgcc ctgtggttca cgctggatcc tcggcacagt
     2101 gagagagaca gggtgcaggc agatgacctg gtgaagatgc tgttcagccc agaagaggtg
     2161 gacctccccc aggaacagcc cctgcctgac cagcagggct cgccagagcc tggagaagag
     2221 tctctgtcag acagagggtc gctgcacaag gatgagctat gacaacagct aggtcacagt
     2281 tgggtgtcct tggactcatg agggtgactc agtccttcag cctggacttc ccggcccctc
     2341 aaggctgcag agtgagaggc gcttctgttg gccccccggc cagaggatcc tgtccacagc
     2401 cttctgcatg gtgctactgt tcttggagtg ggcatagcag gacacactct tctctgggct
     2461 tggctgtagg ctcagaatgc aggcccagaa ccaccctggg gcctatgtag gcagctgccg
     2521 tcagcagcgt gatatattta agtgtctgta aagacaacca aagaataaat gatttgtgtt
     2581 tttttcactt agtggtttgt tccaagagag aaaatatttc tactcttcct gggacgctga
     2641 gcttggtagc agctcctcta ggtagtgtgc cgtggcacat gccacatggg aattcctctg
     2701 tagcctcagg ccagataacc caggtacctt gggctgttac catagaaggg gcatcttggt
     2761 cctcactgcc taccttctat gggaactgga tgtcccagat acagcaccca ggatggcagc
     2821 acatcatcag ctttaaaggc tttcaacatc tagattgtca ctaatcctct tctggcagag
     2881 agcaacagtc agcgctgctc tggcagggca gccgcaggct tcctgctcgg ctggctggcc
     2941 tcagagcccg cacctgactg gagtttcagc tcctccggcc acatagctgg cacgaagccc
     3001 acccccttca gcactcctga gatgctgttg tacggagtta taggaacctg cggctgagta
     3061 gtcattggtc agtcgaacta tttatttagt gagcaccagc ttgttgctgg gaatatgaga
     3121 gggagcaaag caaacctagg cctctcagag cttgtattat ataggggata cataataaat
     3181 gtgtaaatat gtaaaaaaaa aaaaaaaaaa
//
