LOCUS       NM_033321               2426 bp    mRNA    linear   ROD 16-JUN-2024
DEFINITION  Mus musculus purinergic receptor P2X, ligand-gated ion channel, 5
            (P2rx5), transcript variant 1, mRNA.
ACCESSION   NM_033321
VERSION     NM_033321.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Ghnaimawi,S., Zhang,S., Baum,J.I. and Huang,Y.
  TITLE     The Effects of Maternal Intake of EPA and DHA Enriched Diet During
            Pregnancy and Lactation on Offspring's Muscle Development and
            Energy Homeostasis
  JOURNAL   Front Physiol 13, 881624 (2022)
   PUBMED   35733999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2426)
  AUTHORS   Illes,P., Muller,C.E., Jacobson,K.A., Grutter,T., Nicke,A.,
            Fountain,S.J., Kennedy,C., Schmalzing,G., Jarvis,M.F.,
            Stojilkovic,S.S., King,B.F. and Di Virgilio,F.
  TITLE     Update of P2X receptor properties and their pharmacology: IUPHAR
            Review 30
  JOURNAL   Br J Pharmacol 178 (3), 489-514 (2021)
   PUBMED   33125712
  REMARK    Review article
REFERENCE   4  (bases 1 to 2426)
  AUTHORS   Jeong,Y.H., Walsh,M.C., Yu,J., Shen,H., Wherry,E.J. and Choi,Y.
  TITLE     Mice Lacking the Purinergic Receptor P2X5 Exhibit Defective
            Inflammasome Activation and Early Susceptibility to Listeria
            monocytogenes
  JOURNAL   J Immunol 205 (3), 760-766 (2020)
   PUBMED   32540996
  REMARK    GeneRIF: Mice Lacking the Purinergic Receptor P2X5 Exhibit
            Defective Inflammasome Activation and Early Susceptibility to
            Listeria monocytogenes.
REFERENCE   5  (bases 1 to 2426)
  AUTHORS   Kim,H., Walsh,M.C., Takegahara,N., Middleton,S.A., Shin,H.I.,
            Kim,J. and Choi,Y.
  TITLE     The purinergic receptor P2X5 regulates inflammasome activity and
            hyper-multinucleation of murine osteoclasts
  JOURNAL   Sci Rep 7 (1), 196 (2017)
   PUBMED   28298636
  REMARK    GeneRIF: Results identify P2X5 as highly expressed during the
            osteoclasts (OCs) maturation phase and demonstrate that P2X5 is
            required for ATP-mediated inflammasome activation and IL-1beta
            production by OCs, and that P2X5-deficient OC maturation is rescued
            in vitro by addition of exogenous IL-1beta.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2426)
  AUTHORS   Jarvis,M.F. and Khakh,B.S.
  TITLE     ATP-gated P2X cation-channels
  JOURNAL   Neuropharmacology 56 (1), 208-215 (2009)
   PUBMED   18657557
  REMARK    Review article
REFERENCE   7  (bases 1 to 2426)
  AUTHORS   Guo,W., Xu,X., Gao,X., Burnstock,G., He,C. and Xiang,Z.
  TITLE     Expression of P2X5 receptors in the mouse CNS
  JOURNAL   Neuroscience 156 (3), 673-692 (2008)
   PUBMED   18773945
  REMARK    GeneRIF: The identification of extensive P2X5 receptor
            immunoreactivity and mRNA distribution within the CNS of the mouse
            demonstrated here is consistent with a role for extracellular ATP
            acting as a fast neurotransmitter.
REFERENCE   8  (bases 1 to 2426)
  AUTHORS   Shigematsu,Y., Shimoda,Y. and Kaneda,M.
  TITLE     Distribution of immunoreactivity for P2X3, P2X5, and
            P2X6-purinoceptors in mouse retina
  JOURNAL   J Mol Histol 38 (4), 369-371 (2007)
   PUBMED   17605080
  REMARK    GeneRIF: Immunoreactivity for P2X3-, P2X5-, and P2X6-purinoceptors
            was observed in mouse retina.
REFERENCE   9  (bases 1 to 2426)
  AUTHORS   Ryten,M., Yang,S.Y., Dunn,P.M., Goldspink,G. and Burnstock,G.
  TITLE     Purinoceptor expression in regenerating skeletal muscle in the mdx
            mouse model of muscular dystrophy and in satellite cell cultures
  JOURNAL   FASEB J 18 (12), 1404-1406 (2004)
   PUBMED   15231720
  REMARK    GeneRIF: sequential expression of the P2X5, P2Y1, and P2X2
            receptors during the process of muscle regeneration
REFERENCE   10 (bases 1 to 2426)
  AUTHORS   Cox,J.A., Barmina,O. and Voigt,M.M.
  TITLE     Gene structure, chromosomal localization, cDNA cloning and
            expression of the mouse ATP-gated ionotropic receptor P2X5 subunit
  JOURNAL   Gene 270 (1-2), 145-152 (2001)
   PUBMED   11404011
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL670399.4.
            
            On Dec 6, 2019 this sequence version replaced NM_033321.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134662.1, AF333331.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-346               AL670399.4         184249-184594
            347-497             AL670399.4         188717-188867
            498-569             AL670399.4         189401-189472
            570-645             AL670399.4         190004-190079
            646-742             AL670399.4         190166-190262
            743-823             AL670399.4         190423-190503
            824-962             AL670399.4         190774-190912
            963-1096            AL670399.4         191355-191488
            1097-1190           AL670399.4         191740-191833
            1191-1256           AL670399.4         191950-192015
            1257-1339           AL670399.4         194277-194359
            1340-1507           AL670399.4         194944-195111
            1508-2426           AL670399.4         195626-196544
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.25 cM"
     gene            1..2426
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /note="purinergic receptor P2X, ligand-gated ion channel,
                     5"
                     /db_xref="GeneID:94045"
                     /db_xref="MGI:MGI:2137026"
     exon            1..346
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..1577
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     purinergic receptor P2X5-2; P2X purinoceptor 5"
                     /codon_start=1
                     /product="P2X purinoceptor 5 isoform 1"
                     /protein_id="NP_201578.2"
                     /db_xref="CCDS:CCDS24998.1"
                     /db_xref="GeneID:94045"
                     /db_xref="MGI:MGI:2137026"
                     /translation="MGQAAWKGFVLSLFDYKTAKFVVAKSKKVGLLYRVLQLTILLYL
                     LIWVFLIKKSYQDIDTSLQSAVVTKVKGVAYTNTTMLGERLWDVADFVIPSQGENVFF
                     VVTNLIVTPNQRQGICAEREGIPDGECSEDTDCHAGESVVAGHGLKTGRCLRVGNSTR
                     GTCEIFAWCPVETKSMPTDPLLKDAEGFTIFIKNFIRFPKFNFSKANVLETGNKHFLK
                     TCHFSSTNLYCPIFRLGSIVRWAGADFQDIALKGGVIGIHIEWDCDLDKAASHCNPHY
                     YFNRLDNKHTQSISSGYNFRFARYYRDPHGVEFRDLMKAYGIRFDVIVNGKAGKFSII
                     PTVINIGSGLALMGAGAFFCDLVLIYLIRKSEFYRDKKFEKVRGQKEEDNVEVEANEM
                     EQELPEDKPLERVHQDEQALELAQSGRKQNSNCQVLFEPARSGLQENAFVNMKPSQIL
                     QTVKT"
     exon            347..497
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            498..569
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            570..645
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            646..742
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            743..823
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            824..962
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1096
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1190
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1256
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1339
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1507
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..2426
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2402..2407
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /note="hexamer: GATAAA"
     polyA_site      2426
                     /gene="P2rx5"
                     /gene_synonym="P2X5"
                     /note="major polyA site"
ORIGIN      
        1 atcccaggct gggcgctcgc cgcagtcagg ctctccgcgg cagcaggatc ctcctggccg
       61 cgctgtgctc gcgcccgggt ctgcgcgtct gctctccagc tcatgccctt tggagccgcg
      121 cgccccggga ctgcggcagc gcacacagag tgctcggttg gctgccgggc agcgggaggc
      181 ggcggcggtg gccaaagtga gctggaggca tgggccaggc ggcctggaag gggttcgtgt
      241 tgtctctgtt cgactacaag acagcaaagt tcgtggtcgc caagagcaag aaggtggggc
      301 tgctctaccg ggtgctgcag ctcaccatcc tgttgtactt actcatatgg gtgtttctga
      361 taaagaagag ttatcaggac attgacactt ccctgcagag tgctgtggtc accaaagtca
      421 agggggtggc ctataccaac accacgatgc ttggggaacg actctgggat gtggcagact
      481 ttgtcattcc gtctcagggg gagaacgttt tcttcgtggt caccaacctg atcgtgactc
      541 ctaaccagcg gcagggcatc tgtgctgagc gtgaaggcat cccggatggc gagtgttcag
      601 aggacaccga ctgtcacgct ggggagtctg ttgtagcggg acacggactg aaaactggtc
      661 gctgtctacg ggtggggaac tctacccggg gcacctgtga gatctttgct tggtgcccag
      721 tggagacaaa gtccatgcca acggatcccc tcctgaagga cgcagaaggc ttcaccattt
      781 tcataaagaa cttcattcgc ttccccaagt tcaacttctc caaagccaat gtgctagaaa
      841 caggcaacaa acatttcctg aaaacctgtc acttcagctc caccaatctc tactgcccca
      901 tcttccgact ggggtccatt gttcgctggg caggggctga cttccaggac atagccctga
      961 agggcggtgt gataggaatc cacattgaat gggactgtga ccttgataaa gctgcctccc
     1021 actgcaaccc acactattat ttcaaccgtc tggacaacaa acacacacaa tccatctcct
     1081 ctgggtataa cttcaggttt gccaggtatt accgtgaccc tcatggggta gagttccgtg
     1141 acctgatgaa agcatatggc atccgctttg atgtgatagt taatggcaag gcgggaaaat
     1201 tcagcatcat ccccacagtc atcaacattg gttccgggct ggcgctcatg ggtgctgggg
     1261 ctttcttctg tgacctggta cttatctacc tcatcagaaa gagcgagttt taccgagaca
     1321 agaagtttga gaaggtgagg ggtcagaagg aggaagataa tgttgaggtt gaggccaacg
     1381 agatggagca ggagctgcca gaggacaagc cactggagag ggttcatcag gacgagcagg
     1441 ccctggaact ggcccagagt ggcaggaagc agaatagcaa ctgccaggtg ctcttcgagc
     1501 ctgccaggtc tggcctccag gagaatgcct tcgtgaacat gaagccatca cagatcttgc
     1561 aaacagtgaa gacgtagcca gagctcttgg cagggaacag attcaaagat gtctgggcca
     1621 ggacaggcca gggctctgaa ctggggagag gcatctcggt ggtgccggct caggatgtgg
     1681 cttctgagaa caggtccttc aactccaccc agcttgggtg ggatcctgct gcaagatttg
     1741 tttcatggaa ggcttcctgg tggccaaagt ggctcaggat gcaggaagga ggccttgggg
     1801 caggccctca gggaaggggc aggcctgggg tttcagtggt gggtttctgt tcctgactct
     1861 gagcccagct ggacacttgt ttatttatac aggcagtaca catttattgg tgcatgctgc
     1921 attttagtcc tatctgagag acttaaaggc gatgctgacc tggccctagg gtagggggca
     1981 cacaagttaa ctctcaccag agacatcata gtcaacccag ccagcctggc cttcctccag
     2041 cctctagcca agtctttgac aagtccccgc agagaaagac ggctgctcag aaaaagctgt
     2101 tggatgacat ccaggggaca cttagctcca cccccagatg ttaacaagta aagctggcca
     2161 aggaggcacc tggaaaaaag cttaagggga gagcaaacac tcagggctcc taggtaagac
     2221 tggggaagga aggcaagcca gccactacgg gaagcctagc gtggctctgg aaactgactt
     2281 acttgcccag gtgagacatg gacttgtttg tgtggttcag cacttgctgt ttggttggtg
     2341 ctgggggtcc ctgtctgact tccttcctgc tctcagaagc ccagccttga tgtgctaatg
     2401 agataaagga taattttcac tgaaaa
//
