LOCUS       NM_011026               2702 bp    mRNA    linear   ROD 12-AUG-2024
DEFINITION  Mus musculus purinergic receptor P2X, ligand-gated ion channel 4
            (P2rx4), transcript variant 1, mRNA.
ACCESSION   NM_011026
VERSION     NM_011026.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Sierra-Marquez,J., Schaller,L., Sassenbach,L.,
            Ramirez-Fernandez,A., Alt,P., Rissiek,B., Zimmer,B.,
            Schredelseker,J., Hector,J., Stahler,T., Koch-Nolte,F.,
            Staab-Weijnitz,C.A., Dietrich,A., Kopp,R. and Nicke,A.
  TITLE     Different localization of P2X4 and P2X7 receptors in native mouse
            lung - lack of evidence for a direct P2X4-P2X7 receptor interaction
  JOURNAL   Front Immunol 15, 1425938 (2024)
   PUBMED   38953020
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2702)
  AUTHORS   Liu,Z., Sun,M., Liu,W., Feng,F., Li,X., Jin,C., Zhang,Y. and
            Wang,J.
  TITLE     Deficiency of purinergic P2X4 receptor alleviates experimental
            autoimmune hepatitis in mice
  JOURNAL   Biochem Pharmacol 221, 116033 (2024)
   PUBMED   38301964
  REMARK    GeneRIF: Deficiency of purinergic P2X4 receptor alleviates
            experimental autoimmune hepatitis in mice.
REFERENCE   4  (bases 1 to 2702)
  AUTHORS   Brock,V.J., Lory,N.C., Mockl,F., Birus,M., Stahler,T., Woelk,L.M.,
            Jaeckstein,M., Heeren,J., Koch-Nolte,F., Rissiek,B.,
            Mittrucker,H.W., Guse,A.H., Werner,R. and Diercks,B.P.
  TITLE     Time-resolved role of P2X4 and P2X7 during CD8+ T cell activation
  JOURNAL   Front Immunol 15, 1258119 (2024)
   PUBMED   38426095
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2702)
  AUTHORS   Ishikawa,M., Hasanali,Z.S., Zhao,Y., Das,A., Lavaert,M.,
            Roman,C.J., Londregan,J., Allman,D. and Bhandoola,A.
  TITLE     Bone marrow plasma cells require P2RX4 to sense extracellular ATP
  JOURNAL   Nature 626 (8001), 1102-1107 (2024)
   PUBMED   38355795
  REMARK    GeneRIF: Bone marrow plasma cells require P2RX4 to sense
            extracellular ATP.
REFERENCE   6  (bases 1 to 2702)
  AUTHORS   Coutinho-Silva,R., Ojcius,D.M., Gorecki,D.C., Persechini,P.M.,
            Bisaggio,R.C., Mendes,A.N., Marks,J., Burnstock,G. and Dunn,P.M.
  TITLE     Multiple P2X and P2Y receptor subtypes in mouse J774, spleen and
            peritoneal macrophages
  JOURNAL   Biochem Pharmacol 69 (4), 641-655 (2005)
   PUBMED   15670583
REFERENCE   7  (bases 1 to 2702)
  AUTHORS   Ma,B., Ruan,H.Z., Cockayne,D.A., Ford,A.P., Burnstock,G. and
            Dunn,P.M.
  TITLE     Identification of P2X receptors in cultured mouse and rat
            parasympathetic otic ganglion neurones including P2X knockout
            studies
  JOURNAL   Neuropharmacology 46 (7), 1039-1048 (2004)
   PUBMED   15081800
REFERENCE   8  (bases 1 to 2702)
  AUTHORS   Tschop,J., Braun,G.S., Borscheid,R., Horster,M.F. and Huber,S.M.
  TITLE     Ontogeny of purinergic receptor-regulated Ca2+ signaling in mouse
            cortical collecting duct epithelium
  JOURNAL   Cell Physiol Biochem 12 (2-3), 75-82 (2002)
   PUBMED   12077552
REFERENCE   9  (bases 1 to 2702)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2702)
  AUTHORS   Townsend-Nicholson,A., King,B.F., Wildman,S.S. and Burnstock,G.
  TITLE     Molecular cloning, functional characterization and possible
            cooperativity between the murine P2X4 and P2X4a receptors
  JOURNAL   Brain Res Mol Brain Res 64 (2), 246-254 (1999)
   PUBMED   9931497
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY150918.1, AK162820.1, AF089751.1, BM244734.2 and AC115728.11.
            
            On Jul 18, 2015 this sequence version replaced NM_011026.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK162820.1, AK150351.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                BY150918.1         2-49
            49-1225             AK162820.1         2-1178
            1226-1654           AF089751.1         1147-1575
            1655-2047           AK162820.1         1606-1998
            2048-2048           BM244734.2         29-29               c
            2049-2339           AK162820.1         2000-2290
            2340-2340           AC115728.11        134883-134883       c
            2341-2702           AK162820.1         2292-2653
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 62.53 cM"
     gene            1..2702
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /note="purinergic receptor P2X, ligand-gated ion channel
                     4"
                     /db_xref="GeneID:18438"
                     /db_xref="MGI:MGI:1338859"
     exon            1..244
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..1277
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /note="isoform 1 is encoded by transcript variant 1; P2X
                     purinoceptor 4; ionotropic purinergic receptor"
                     /codon_start=1
                     /product="P2X purinoceptor 4 isoform 1"
                     /protein_id="NP_035156.2"
                     /db_xref="CCDS:CCDS19654.2"
                     /db_xref="GeneID:18438"
                     /db_xref="MGI:MGI:1338859"
                     /translation="MAGCCSVLRAFLFEYDTPRIVLIRSRKVGLMNRVVQLLILAYVI
                     GWVFVWEKGYQETDSVVSSVTTKAKGVAVTNTSQLGFRIWDVADYVVPAQEENSLFIM
                     TNMIVTVNQTQGTCPEIPDKTSICDSDANCTLGSSDTHSSGIGTGRCVPFNASVKTCE
                     VAAWCPVENDAGVPTPAFLKAAENFTLLVKNNIWYPKFNFSKRNILPNITTSYLKSCI
                     YNARTDPFCPIFRLGQIVADAGHSFQEMAVEGGIMGIQIKWDCNLDRAASHCLPRYSF
                     RRLDTRDLEHNVSPGYNFRFAKYYRDLAGNEQRTLTKAYGIRFDIIVFGKAGKFDIIP
                     TMINVGSGLALLGVATVLCDVIVLYCMKKRYYYRDKKYKYVEDYEQGLSGETDQ"
     exon            245..392
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            393..464
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            465..537
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            538..634
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            635..715
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            716..857
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            858..994
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1088
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1154
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1250
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..2702
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1988..1993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /note="hexamer: AAGAAA"
     polyA_site      2008
                     /gene="P2rx4"
                     /gene_synonym="D5Ertd444e; P2X4"
                     /note="major polyA site"
ORIGIN      
        1 atttccgggc cgagctgctg ggcggggctg ctgcccggac ccggtggtgg tggtggcagc
       61 gacaaggcga cccgcggtgg ccacgacgcg gagcagcggg cggcggagcc atggcaggct
      121 gctgctccgt gctccgggct ttcctgttcg agtacgacac gccgcgcatc gtgctcatcc
      181 gcagccgtaa agtggggctc atgaaccgcg tggtgcagct gctcatcctg gcttacgtca
      241 ttgggtgggt gttcgtgtgg gaaaagggct accaggaaac ggactctgtg gtcagctcgg
      301 tgacaaccaa agccaaaggt gtggctgtga ccaacacttc tcagcttgga ttccggatct
      361 gggacgtggc cgactatgtg gtcccagctc aggaggaaaa ctccctcttc atcatgacca
      421 acatgatcgt caccgtgaac cagacgcagg gtacctgtcc agagattcct gataagacca
      481 gcatttgcga ttcagacgcc aactgcactc tcggctcctc ggacacccac agcagtggaa
      541 ttgggactgg aaggtgtgtt ccattcaacg cgtctgtgaa gacctgtgag gtggccgcat
      601 ggtgcccagt ggagaatgac gctggtgtgc caacgccagc ttttttaaag gctgcagaaa
      661 acttcaccct cttggtaaag aacaacatct ggtaccccaa gtttaatttc agcaagagga
      721 atatcctccc caacattacc acctcctacc tcaagtcgtg catttataat gcgcggacgg
      781 atcccttctg ccccatattc cgtcttggcc aaatcgtggc ggatgcgggc cacagctttc
      841 aggagatggc agtggaggga ggcatcatgg gtatccagat caagtgggac tgcaaccttg
      901 acagagctgc ctcccactgc ctgcccagat attccttccg gcgcctcgac actcgggact
      961 tagaacacaa cgtgtctcct ggctacaatt tcaggtttgc caagtactac agggaccttg
     1021 ctggcaatga gcaacgcaca ctcaccaagg catatggcat ccgctttgac atcatcgtgt
     1081 ttggaaaggc tggcaagttt gacatcatcc caaccatgat caatgttggc tctggcttgg
     1141 cgctcctagg ggtggcgacc gtgctgtgtg acgtcatagt cctctactgt atgaagaaga
     1201 gatactacta ccgggacaag aaatataagt atgtggaaga ctacgagcag ggtctttccg
     1261 gagagacgga ccagtgatgc ctaaccctac atttcaatcc cactcagcct gtgatgcaga
     1321 aatatgggag acttggctgc tgcgtctgtc actctagaga cggttccaga gtctcactcg
     1381 gtctccactc cacaaatact cagggttgcc cgagcacgtc ttgtattgtt tttttgtggg
     1441 ttttgagccc gggtcctgct ctgcagctca gatgggcttc agattcaaga tcctcctgct
     1501 tcaacctcca ggggtgctgg aatcccacac gtccataaca gcacccactt cccacgggag
     1561 ttccggcatt tttacatttt acaggttcat tgtacacatc tcaggctggc ctcaaactcc
     1621 aagacatcca agaaacccta ttaatctcat tgtgcatatg gggggggggt catttgcaca
     1681 ctacacacaa tggatttctg gtgtgctgtt ggctgggtca cctgttgctt atacaatgtg
     1741 agtgtactga ggtaagggga atatgggagc agagacacgg ctgtggctta cggacaggcc
     1801 cagtccttgg aggcacagca gctctgtcca agcactttat aaggaaggag gctcccccag
     1861 gtgccgtcag gaggcctggg atactattcc tcttccctat gaccagagaa gttgtcctcg
     1921 gaacaaaggc agggttagct tttcctttca taagggctgt gttgaaatga cctaggacca
     1981 aacattaaag aaaataatgt ttttaaaaga tccttgtctg tgctgcctcc cggcttgtga
     2041 gccggatccg ccttacatta ttaaagttgt cagcatacaa acggagccac caaacaaggc
     2101 tggggtggga ggtccttgtg tggtatctgc ctgttggtaa gattatgtat atgcacattc
     2161 ttgcagtgaa ggtcacttcc aggagcagct agattttcta tccgctgctt ccgtctctgc
     2221 ctgaggtaaa tccgtgacca gaacttgtac ttcccaacag cggcataagt cctcttctta
     2281 cctttcaaag tttgccttgg ctttctggct gtaacaacat gccgcctctg tcctgcccta
     2341 acccacaggg cactcgcatc ccacacggca ccctccacca tctctaaaga gactccttgc
     2401 tttggagagc ctcttcctgt cagtatttgt ctgtgacagc ctcctgtggg ctagccagcc
     2461 atgctcagga aagctcacgt tgcttctggc aagaggtttt gtttcttatg gcgctggggg
     2521 caagacttat gaaatggttt taaggagacg ctggtttgtg ttgtttgttt gtttttgaga
     2581 cagggtttct ctgtgtagcc ctggctgtcc tggaactcaa gctgtagacc aggctggctt
     2641 tgaactccta gaaacctgcc tgcctctgcc ttcaagtacg ggaattaaaa gcgtgcagca
     2701 cc
//
