LOCUS       NM_133985               4647 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus oxidative-stress responsive 1 (Oxsr1), transcript
            variant 1, mRNA.
ACCESSION   NM_133985 XM_135264 XM_902947 XM_916586 XM_924327 XM_993229
            XM_993249
VERSION     NM_133985.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Biggs O'May,J., Vanes,L., de Boer,L.L., Lewis,D.A., Hartweger,H.,
            Kunzelmann,S., Hayward,D., Llorian,M., Kochl,R. and
            Tybulewicz,V.L.J.
  TITLE     WNK1-dependent water influx is required for CD4+ T cell activation
            and T cell-dependent antibody responses
  JOURNAL   Nat Commun 16 (1), 1857 (2025)
   PUBMED   39984435
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   de Boer,L.L., Vanes,L., Melgrati,S., Biggs O'May,J., Hayward,D.,
            Driscoll,P.C., Day,J., Griffiths,A., Magueta,R., Morrell,A.,
            MacRae,J.I., Kochl,R. and Tybulewicz,V.L.J.
  TITLE     T cell migration requires ion and water influx to regulate actin
            polymerization
  JOURNAL   Nat Commun 14 (1), 7844 (2023)
   PUBMED   38057317
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4647)
  AUTHORS   He,Z., Xu,L., Zeng,X., Yang,B., Liu,P., Han,D., Xue,H. and Luo,B.
  TITLE     circROCK1 Promotes septic myocardial injury through regulating
            miR-96-5p/OXSR1 axis
  JOURNAL   Acta Biochim Pol 70 (3), 567-574 (2023)
   PUBMED   37721476
  REMARK    GeneRIF: circROCK1 Promotes septic myocardial injury through
            regulating miR-96-5p/OXSR1 axis.
REFERENCE   4  (bases 1 to 4647)
  AUTHORS   Hayward,D.A., Vanes,L., Wissmann,S., Sivapatham,S., Hartweger,H.,
            Biggs O'May,J., de Boer,L.L., Mitter,R., Kochl,R., Stein,J.V. and
            Tybulewicz,V.L.J.
  TITLE     B cell-intrinsic requirement for WNK1 kinase in antibody responses
            in mice
  JOURNAL   J Exp Med 220 (3) (2023)
   PUBMED   36662229
REFERENCE   5  (bases 1 to 4647)
  AUTHORS   Kochl,R., Vanes,L., Llorian Sopena,M., Chakravarty,P.,
            Hartweger,H., Fountain,K., White,A., Cowan,J., Anderson,G. and
            Tybulewicz,V.L.
  TITLE     Critical role of WNK1 in MYC-dependent early mouse thymocyte
            development
  JOURNAL   Elife 9, e56934 (2020)
   PUBMED   33051000
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4647)
  AUTHORS   Vallon,V.
  TITLE     Regulation of the Na+-Cl- cotransporter by dietary NaCl: a role for
            WNKs, SPAK, OSR1, and aldosterone
  JOURNAL   Kidney Int 74 (11), 1373-1375 (2008)
   PUBMED   19008908
  REMARK    GeneRIF: Regulation of the Na+-Cl- cotransporter by dietary NaCl: a
            role for WNKs, SPAK, OSR1, and aldosterone.
REFERENCE   7  (bases 1 to 4647)
  AUTHORS   Chiga,M., Rai,T., Yang,S.S., Ohta,A., Takizawa,T., Sasaki,S. and
            Uchida,S.
  TITLE     Dietary salt regulates the phosphorylation of OSR1/SPAK kinases and
            the sodium chloride cotransporter through aldosterone
  JOURNAL   Kidney Int 74 (11), 1403-1409 (2008)
   PUBMED   18800028
  REMARK    GeneRIF: phosphorylation states of NCC and OSR1/SPAK (Gene: Oxsr1,
            GeneID: 108737) were increased by low-salt diets and decreased by
            high-salt diets
REFERENCE   8  (bases 1 to 4647)
  AUTHORS   Gagnon,K.B., England,R. and Delpire,E.
  TITLE     Characterization of SPAK and OSR1, regulatory kinases of the
            Na-K-2Cl cotransporter
  JOURNAL   Mol Cell Biol 26 (2), 689-698 (2006)
   PUBMED   16382158
  REMARK    GeneRIF: Characterization of SPAK and OSR1, regulatory kinases of
            the Na-K-2Cl cotransporter.
REFERENCE   9  (bases 1 to 4647)
  AUTHORS   Chen,W., Yazicioglu,M. and Cobb,M.H.
  TITLE     Characterization of OSR1, a member of the mammalian Ste20p/germinal
            center kinase subfamily
  JOURNAL   J Biol Chem 279 (12), 11129-11136 (2004)
   PUBMED   14707132
REFERENCE   10 (bases 1 to 4647)
  AUTHORS   Piechotta,K., Lu,J. and Delpire,E.
  TITLE     Cation chloride cotransporters interact with the stress-related
            kinases Ste20-related proline-alanine-rich kinase (SPAK) and
            oxidative stress response 1 (OSR1)
  JOURNAL   J Biol Chem 277 (52), 50812-50819 (2002)
   PUBMED   12386165
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC128702.4 and BC060645.1.
            
            On Dec 24, 2011 this sequence version replaced NM_133985.1.
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060645.1, AK129291.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AC128702.4         66208-66224
            18-3796             BC060645.1         1-3779
            3797-4647           AC128702.4         149353-150203
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 71.33 cM"
     gene            1..4647
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /note="oxidative-stress responsive 1"
                     /db_xref="GeneID:108737"
                     /db_xref="MGI:MGI:1917378"
     exon            1..423
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     CDS             354..1937
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /EC_number="2.7.11.1"
                     /note="oxidative stress-responsive 1 protein"
                     /codon_start=1
                     /product="serine/threonine-protein kinase OSR1"
                     /protein_id="NP_598746.2"
                     /db_xref="CCDS:CCDS57714.1"
                     /db_xref="GeneID:108737"
                     /db_xref="MGI:MGI:1917378"
                     /translation="MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKERVA
                     IKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLD
                     IIKHIVAKGEHKSGVLDEPTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSV
                     QIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAI
                     ELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDP
                     EKRPTAAELLRHKFFQKAKNKEFLQEKILQRAPTISERSKKVRRVPGSSGRLHKTEDG
                     GWEWSDDEFDEESEEGRAAISQLRSPRVKDSLSSSELFAAAEPMGTLLQVPEQISAHL
                     PQPAGQMPTQPAQVSLLPPAEPAKPAQAQSSGERSQETKIPISLVLRLRNSKKELNDI
                     RFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVE
                     GSDIPDDGKLIGFAQLSIS"
     exon            424..536
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..645
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            646..787
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            788..843
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            844..953
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1055
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1189
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1238
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1304
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1427
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1463
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1610
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1675
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1728
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1797
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1862
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..4647
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2386..2391
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /note="hexamer: ATTAAA"
     polyA_site      2409
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /note="major polyA site"
     regulatory      4614..4619
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /note="hexamer: AATAAA"
     regulatory      4621..4626
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /note="hexamer: AGTAAA"
     polyA_site      4641
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
                     /note="major polyA site"
     polyA_site      4647
                     /gene="Oxsr1"
                     /gene_synonym="2210022N24Rik; 2810422B09Rik; mKIAA1101;
                     Osr1"
ORIGIN      
        1 gtgtcggcag gtggagggag aagggaaagt ttggcccgtg cttggggttc gggtggaggg
       61 cgggacggag gggcagggct gggtagaagc ttgtttcgcg gtgccgcgga ggttaaggcc
      121 ggctcgtggg ctgggccggg gactgcggga cgcggagccg cgggaggcga ggtcggtcgc
      181 agccgcgcgt ctccgcctcg gggccgcgct tgacccgcgc gccccggcgt tgtgcgcccc
      241 aaggctgggc caagcggcgg gttgggggcg gcggctgggc gtggggaggc gcgcggcgag
      301 gtgacctgag agcccggagc gtgaggagca cggagccagg cgctgcagcc gtcatgtccg
      361 aggactcgag cgccctgccc tggtccatca acagggacga ctacgagctg caggaggtga
      421 tagggagtgg agcaactgcc gtggtccaag cagcttactg tgcccccaaa aaggagagag
      481 tggcgatcaa gcggataaac ctggagaagt gtcagaccag catggatgag ctcctgaaag
      541 aaatccaggc catgagccaa tgccatcatc ctaacattgt gtcttactac acatcttttg
      601 tggtaaagga tgagctgtgg ctcgtcatga agctgctaag tggaggttct gttctggata
      661 ttattaaaca cattgtggca aagggggaac ataagagtgg agttctggac gagccaacca
      721 ttgctacaat cctccgagaa gtcttagaag gactggagta cctgcataag aatgggcaga
      781 ttcacagaga tgtgaaagct ggaaacattc ttctaggaga agacggctct gtgcagattg
      841 cagatttcgg cgtcagtgct tttttagcca ctggtggtga cattaccaga aataaagtga
      901 gaaaaacgtt tgttggcacc ccgtgctgga tggcacccga agttatggag caggttcgag
      961 gttatgattt taaagctgat atttggagtt ttggaatcac tgcgattgaa ctggccacag
     1021 gggcagctcc ttatcataaa tatccaccaa tgaaggtttt aatgctgacg ctccagaatg
     1081 atcctccttc tttggaaact ggtgttcaag ataaagaaat gctgaagaaa tatggaaaat
     1141 catttagaaa aatgatttca ttgtgccttc aaaaggatcc agaaaaaagg ccaacagcag
     1201 cagaactgtt gaggcacaaa tttttccaga aagcaaagaa taaagaattt cttcaagaaa
     1261 aaatactcca gagagcacct accatttctg aaagatctaa aaaggtccgc agagtccccg
     1321 gctccagtgg ccgtctccat aagacagagg acggaggctg ggagtggagt gacgatgagt
     1381 ttgatgagga gagcgaggaa gggagagctg ccatttctca gctgaggtct ccccgagtaa
     1441 aagactcatt atccagttct gagctcttcg cggcagctga gcccatgggg actttgcttc
     1501 aagttccaga acagatttct gctcatctac ctcagccagc tggccagatg cccacacagc
     1561 cagctcaagt ctctctcctg ccccctgcag agccagcaaa accagcacag gctcagtctt
     1621 caggagaacg gtcccaggag accaagatcc ctatcagtct agtgctaaga ttaaggaatt
     1681 ccaaaaaaga actaaatgat attcgatttg aatttactcc tgggagagat acagcagagg
     1741 gtgtttctca ggagctcatt tctgctggcc tggttgatgg aagggattta gtaatagtgg
     1801 cagccaatct gcagaagatt gtggaagaac ctcagtcaaa ccgatctgtc actttcaaac
     1861 tggcatctgg tgttgaaggc tcggatattc ctgatgatgg caaactgata ggatttgccc
     1921 agctcagcat cagctaaacc acagacctgg aagagtcagc ctaggagatg ccacacatgc
     1981 acatgtctgt tgcttctgtt ggcctgaacc tacaactgcc aaagaaccca gcaacaaacc
     2041 tcccagcttg gagctttagt ttttttcttt gtttttcttg ctgccctcct ccctgccccc
     2101 cttttcttac aaggaaagaa aagttggatc gtcagtggcc agcctcccct cagagagttc
     2161 cgttggtaga tacgctgctc atgtcccctc tccctccatc tgagaagcag aagcggccag
     2221 tgtgcctcaa ggcccaggat ggaagactct caactcattc ttgccttact acagtgatgg
     2281 tcccagtgga tagagcagct gcactgggca acctcatctt gcctgctctc ccgcacctgc
     2341 tgggacatga gcatcttggg atatctcttt aacactcaag tagcaattaa agttgattgt
     2401 tcagctggag cccagagaat ttaattactg tttttttctt tgtacctgat gtgaattcta
     2461 gcaacctttg ttaggaaaaa gcacagcctc gaatggaggc agcctaaact cttgtctgtc
     2521 ctcttcatgg tgtctcttcc gtgttttgct gagctgttct caggcacccc ctcactgctc
     2581 cctcttgaaa gggttgctag ccttaacttc agctggtgcg aaacatctga ctgttgtcag
     2641 actccagcca tctgctcctg cagcgtgcag ctttggattg tttgtgcaga aaacagcaag
     2701 cgatggctga taaatcttat cttgccagag gtagtttttg aacaagaaaa agaactttgt
     2761 aaatcatatc attgggaaag tattttcaat atttaaaaaa attaagctga agaaaaactg
     2821 atctttgatc ctcctgtgaa gatggacttg cctggcctta gtcatgtgga aatggtgtac
     2881 ccggctgaga tttttacagc aataaagggg actctcacta ggctagaggc cgccttctgc
     2941 tcctctcttg cactgactct gtggaggggg tcttcattca ctaaactgac ccaggatgga
     3001 acacgaaacc acacgtgccg tgacctttag gtttatgagt agacagtgtt aatttgattt
     3061 tctacaggaa ttatgtaact tattctttag gtttaaaaag aatgctcaaa ggctatacag
     3121 agaagccctg tatcaaaaac caaaccaaac aaacgaacaa gatcaatgca atatgggaat
     3181 aatcagtgag gttggctttt ttccctactg ttaaatagca tttgtctaat tgctaataag
     3241 cctgtcgtta ccaaatttta tataagaatg gaatagtcag tctggcctta aagagacaca
     3301 caaagatggg ctgtgagccc ctagaaaggg actagttagt tgcctttgat agaattcctt
     3361 gcacctgcca ttgttggttt gatgaacacg attaggtacc cagtgtgatc ccaccctcac
     3421 cccacctcac ccagagcaaa ccgtaggact gctcctagaa gcagctctgt tgacttctct
     3481 tggaacacac ctgaacgctt tcctcatttc taacctgtgc cacctcttgt ttcgttcatg
     3541 ctgagcctgt agtttcagtc atggactttt gtactgttga gtgcaaaggc atgttgggaa
     3601 aggtgttggg gaggaagaga agatggattt gagaggggag ttgggagact agagagttca
     3661 aggtcccagc aggttggtaa aaggaggagc ctaccaagtt aggagggaga aaaaagatta
     3721 accagctagt tagaaaacca tttatttttc tttcttcttt ccccacttgt tagaggtagg
     3781 gctatgagga agcaaggatg ggaggtgggg ttgaggtttg ggaagcaggc agcaagcgtt
     3841 acctttgcag aagactcgtg tcttgctgta gaatccagtt tggtttcagc agcttgactt
     3901 aatgcactta caggctcagg aaggaatttg ctatcactgt tgtggctttt gacttggggt
     3961 ggtccagtgc caggctggaa gtaagcacag cccatctcct gttcacattc tctagaccgt
     4021 catcaccttt gccctgctct gggggacaga catgacagta aaagtgtccc gggactcttt
     4081 ccgttcattg tttgcttttt tggcaactag gaggttggca ctaagaccca ggacaatgtt
     4141 acttctctgt gatgtgtggt gacttccctc tgctggctta tgcagtgatg gtgaatgaac
     4201 cagtcagtgg aaaactgctg aggtgggaca gctgggagcc agggagccga agcagtgctt
     4261 cagggggtac tgtcactaag ccctcaagat acccttgtcc ctgaagtagg gactaactat
     4321 agcacaagat agtatgtgcc aatatagcca agataatatg tgaaatggtc tttctaaatg
     4381 attaaagaaa tcatttgcgt tggttcttgt tttttgtgtt ttccctctat tttcaaccaa
     4441 atcctagagc cagctgagac tgctgagtaa tctgaaggcg agagcaagga tataccagcg
     4501 aggtcagccc ctctagaatg tttgctggat ggtggaagat gtaaaatatt accttctccc
     4561 tctcctcttg acttgtattt tactgcatgg tttttttttt ttcattttta atcaataaag
     4621 agtaaattgt ccttaatgat atataaa
//
