LOCUS       NM_001416047            1432 bp    mRNA    linear   ROD 09-JUL-2024
DEFINITION  Mus musculus OTU deubiquitinase with linear linkage specificity
            (Otulin), transcript variant 5, mRNA.
ACCESSION   NM_001416047
VERSION     NM_001416047.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Staels,F., Bucken,L., De Vuyst,L., Willemsen,M., Van
            Nieuwenhove,E., Gerbaux,M., Neumann,J., Malviya,V., Van
            Meerbeeck,L., Haughton,J., Seldeslachts,L., Gouwy,M., Martinod,K.,
            Vande Velde,G., Proost,P., Yshii,L., Schlenner,S., Schrijvers,R.,
            Liston,A. and Humblet-Baron,S.
  TITLE     OTULIN haploinsufficiency predisposes to environmentally directed
            inflammation
  JOURNAL   Front Immunol 15, 983686 (2024)
   PUBMED   38827742
  REMARK    GeneRIF: OTULIN haploinsufficiency predisposes to environmentally
            directed inflammation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1432)
  AUTHORS   Doglio,M.G., Verboom,L., Ruilova Sosoranga,E., Frising,U.C.,
            Asaoka,T., Gansemans,Y., Van Nieuwerburgh,F., van Loo,G. and
            Wullaert,A.
  TITLE     Myeloid OTULIN deficiency couples RIPK3-dependent cell death to
            Nlrp3 inflammasome activation and IL-1beta secretion
  JOURNAL   Sci Immunol 8 (89), eadf4404 (2023)
   PUBMED   38000038
REFERENCE   4  (bases 1 to 1432)
  AUTHORS   Verboom,L., Anderson,C.J., Jans,M., Petta,I., Blancke,G.,
            Martens,A., Sze,M., Hochepied,T., Ravichandran,K.S., Vereecke,L.
            and van Loo,G.
  TITLE     OTULIN protects the intestinal epithelium from apoptosis during
            inflammation and infection
  JOURNAL   Cell Death Dis 14 (8), 534 (2023)
   PUBMED   37598207
  REMARK    GeneRIF: OTULIN protects the intestinal epithelium from apoptosis
            during inflammation and infection.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1432)
  AUTHORS   Almeida,S.M., Ivantsiv,S., Niibori,R., Dunham,W.H., Green,B.A.,
            Zhao,L., Gingras,A.C. and Cordes,S.P.
  TITLE     An interaction between OTULIN and SCRIB uncovers roles for linear
            ubiquitination in planar cell polarity
  JOURNAL   Dis Model Mech 16 (8) (2023)
   PUBMED   37589075
REFERENCE   6  (bases 1 to 1432)
  AUTHORS   van Wijk,S.J.L., Fricke,F., Herhaus,L., Gupta,J., Hotte,K.,
            Pampaloni,F., Grumati,P., Kaulich,M., Sou,Y.S., Komatsu,M.,
            Greten,F.R., Fulda,S., Heilemann,M. and Dikic,I.
  TITLE     Linear ubiquitination of cytosolic Salmonella Typhimurium activates
            NF-kappaB and restricts bacterial proliferation
  JOURNAL   Nat Microbiol 2, 17066 (2017)
   PUBMED   28481361
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1432)
  AUTHORS   Damgaard,R.B., Walker,J.A., Marco-Casanova,P., Morgan,N.V.,
            Titheradge,H.L., Elliott,P.R., McHale,D., Maher,E.R.,
            McKenzie,A.N.J. and Komander,D.
  TITLE     The Deubiquitinase OTULIN Is an Essential Negative Regulator of
            Inflammation and Autoimmunity
  JOURNAL   Cell 166 (5), 1215-1230 (2016)
   PUBMED   27523608
REFERENCE   8  (bases 1 to 1432)
  AUTHORS   Takiuchi,T., Nakagawa,T., Tamiya,H., Fujita,H., Sasaki,Y.,
            Saeki,Y., Takeda,H., Sawasaki,T., Buchberger,A., Kimura,T. and
            Iwai,K.
  TITLE     Suppression of LUBAC-mediated linear ubiquitination by a specific
            interaction between LUBAC and the deubiquitinases CYLD and OTULIN
  JOURNAL   Genes Cells 19 (3), 254-272 (2014)
   PUBMED   24461064
REFERENCE   9  (bases 1 to 1432)
  AUTHORS   Rivkin,E., Almeida,S.M., Ceccarelli,D.F., Juang,Y.C., MacLean,T.A.,
            Srikumar,T., Huang,H., Dunham,W.H., Fukumura,R., Xie,G., Gondo,Y.,
            Raught,B., Gingras,A.C., Sicheri,F. and Cordes,S.P.
  TITLE     The linear ubiquitin-specific deubiquitinase gumby regulates
            angiogenesis
  JOURNAL   Nature 498 (7454), 318-324 (2013)
   PUBMED   23708998
  REMARK    GeneRIF: two distinct mutations in the (uro)chordate-specific gumby
            (also called Fam105b) gene cause an embryonic angiogenic phenotype
            in gumby mice
REFERENCE   10 (bases 1 to 1432)
  AUTHORS   Mar,L., Rivkin,E., Kim,D.Y., Yu,J.Y. and Cordes,S.P.
  TITLE     A genetic screen for mutations that affect cranial nerve
            development in the mouse
  JOURNAL   J Neurosci 25 (50), 11787-11795 (2005)
   PUBMED   16354937
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158955.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422589.425483.1,
                                           SRR13422592.226558.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-254               AC158955.2         195589-195842       c
            255-349             AC158955.2         184664-184758       c
            350-493             AC158955.2         181459-181602       c
            494-619             AC158955.2         176647-176772       c
            620-889             AC158955.2         173727-173996       c
            890-1432            AC158955.2         171054-171596       c
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 10.31 cM"
     gene            1..1432
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /note="OTU deubiquitinase with linear linkage specificity"
                     /db_xref="GeneID:432940"
                     /db_xref="MGI:MGI:3577015"
     exon            1..254
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /inference="alignment:Splign:2.1.0"
     exon            255..349
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..1084
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /EC_number="3.4.19.12"
                     /note="isoform 4 is encoded by transcript variant 5;
                     protein FAM105B; gumby; ubiquitin thioesterase Gumby;
                     deubiquitinating enzyme otulin; OTU domain-containing
                     deubiquitinase with linear linkage specificity; ubiquitin
                     thioesterase otulin; family with sequence similarity 105,
                     member B"
                     /codon_start=1
                     /product="ubiquitin thioesterase otulin isoform 4"
                     /protein_id="NP_001402976.1"
                     /db_xref="GeneID:432940"
                     /db_xref="MGI:MGI:3577015"
                     /translation="MDIMDYCKKEWRGNTQKATCMKKGYEEVSQKFTSIRRVRGDNYC
                     ALRATLFQAMSQLAELPPWLQDLELILLPEKLINKYTWIKQWKLGLKFDGKSEDLVEK
                     IKESLALLRKKWVSLAAMKTAEARQTACDELFTNEEEEYSLYEAVKFLMLNRAIELYD
                     DKEKGKEVPFFSVLLFARDTSNDPEQLLRNHLNQVGHTGGLEQVEMFLLAYAVRHSIR
                     VYRLSKYNTEEFITVYPTDPPKDWPMVTLIAEDDRHYNIPVRVCEETSV"
     exon            350..493
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /inference="alignment:Splign:2.1.0"
     exon            494..619
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /inference="alignment:Splign:2.1.0"
     exon            620..889
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1432
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1388..1393
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /note="hexamer: AATGAA"
     polyA_site      1432
                     /gene="Otulin"
                     /gene_synonym="Fam105b; m3Sapc; m7-1Sapc"
                     /note="major polyA site"
ORIGIN      
        1 agtgacgctt acggaagcgc tctgcggacc tcgcggggtg cggggcagcg cagccacggc
       61 tccggacggc gggacggcag ggcccggcgt ggccgcggat gagtcgggga accatgcccc
      121 agcccggagc gtggcccggt gcaagctgcg ccgagacgcc ggcgcgcgag gccggggccg
      181 cggcgcggga cggcgggaag gtgacggccg gcgcgcagcc ccgggccgcg acgcgatgcc
      241 cggccgagca gtgaaaccca ggttaagtgt ggctcctgaa atggatatca tggactactg
      301 caaaaaagaa tggagaggaa atactcagaa agccacatgt atgaaaaagg gctatgagga
      361 agtgtctcag aaattcactt ccataaggcg agtccgtggt gataactact gtgcactgag
      421 ggccacactg ttccaggcca tgagccagct ggccgagctg cccccctggc tgcaggactt
      481 ggagctcata ctgttaccag agaagctgat aaacaagtat acctggatca agcagtggaa
      541 acttggactg aaatttgatg ggaagagtga ggacctggtt gaaaaaatta aggaatccct
      601 tgccctgcta aggaagaagt gggtaagcct ggctgcaatg aaaactgctg aagcgaggca
      661 gacagcttgt gatgagctgt tcaccaacga agaggaggag tacagcctct acgaagctgt
      721 gaagttcctg atgttaaaca gagccatcga actgtacgat gacaaggaga agggaaagga
      781 agtgccgttc ttctctgtgc tcttgtttgc ccgagacaca tccaatgacc ctgaacagct
      841 cctgaggaac cacctaaacc aggtgggaca cacggggggc cttgagcagg ttgagatgtt
      901 ccttcttgcc tatgctgtcc gccacagcat ccgggtgtac cggctgtcca agtataacac
      961 agaggagttc atcacagtct accccactga tccccccaag gactggccaa tggtgaccct
     1021 catagctgag gatgatcggc actataacat ccctgtcaga gtgtgtgagg agaccagtgt
     1081 gtgagggaca cacatgtgac tgccaagaga tgcgcatgtg cagctcctcc tctgggggcc
     1141 tcagatctgc aggtctccaa ggacaatctg ggagaactct cgggcccctg gagccaactt
     1201 tggaccatta tgttctgaag actagctgtt gataatgaga attaattagc caagccttca
     1261 ctccttgttc tgttatgcag tgtgttccac tgggggcttc agcacgtgcc tttggaaggt
     1321 gcaaactatg tctccataag gcggatgctt ttgggtcaaa gaaaactgtt tggaaaggaa
     1381 tctgtccaat gaaagttctg acggtaaact ggttttactt ctctttttaa aa
//
