LOCUS       NM_001256033            7327 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus OTU domain containing 4 (Otud4), transcript variant 1,
            mRNA.
ACCESSION   NM_001256033
VERSION     NM_001256033.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Wang,F., Yin,X., Fan,Y.M., Zhang,X., Ma,C., Jia,K., Zhou,W.,
            Tang,Z., Qi,L.W. and Li,J.
  TITLE     Upregulation of glycolytic enzyme PFKFB3 by deubiquitinase OTUD4
            promotes cardiac fibrosis post myocardial infarction
  JOURNAL   J Mol Med (Berl) 101 (6), 743-756 (2023)
   PUBMED   37162556
  REMARK    GeneRIF: Upregulation of glycolytic enzyme PFKFB3 by deubiquitinase
            OTUD4 promotes cardiac fibrosis post myocardial infarction.
REFERENCE   3  (bases 1 to 7327)
  AUTHORS   Kedia,S., Aghanoori,M.R., Burns,K.M.L., Subha,M., Williams,L.,
            Wen,P., Kopp,D., Erickson,S.L., Harvey,E.M., Chen,X., Hua,M.,
            Perez,J.U., Ishraque,F. and Yang,G.
  TITLE     Ubiquitination and deubiquitination of 4E-T regulate neural
            progenitor cell maintenance and neurogenesis by controlling P-body
            formation
  JOURNAL   Cell Rep 40 (2), 111070 (2022)
   PUBMED   35830814
REFERENCE   4  (bases 1 to 7327)
  AUTHORS   Liu,H., Fan,J., Zhang,W., Chen,Q., Zhang,Y. and Wu,Z.
  TITLE     OTUD4 alleviates hepatic ischemia-reperfusion injury by suppressing
            the K63-linked ubiquitination of TRAF6
  JOURNAL   Biochem Biophys Res Commun 523 (4), 924-930 (2020)
   PUBMED   31964525
  REMARK    GeneRIF: OTUD4 alleviates hepatic ischemia-reperfusion injury by
            suppressing the K63-linked ubiquitination of TRAF6.
REFERENCE   5  (bases 1 to 7327)
  AUTHORS   Liuyu,T., Yu,K., Ye,L., Zhang,Z., Zhang,M., Ren,Y., Cai,Z., Zhu,Q.,
            Lin,D. and Zhong,B.
  TITLE     Induction of OTUD4 by viral infection promotes antiviral responses
            through deubiquitinating and stabilizing MAVS
  JOURNAL   Cell Res 29 (1), 67-79 (2019)
   PUBMED   30410068
  REMARK    GeneRIF: The authors show an essential role of OTUD4 in
            virus-triggered signaling and contribute to the understanding of
            deubiquitination-mediated regulation of innate antiviral responses.
REFERENCE   6  (bases 1 to 7327)
  AUTHORS   Zhao,Y., Mudge,M.C., Soll,J.M., Rodrigues,R.B., Byrum,A.K.,
            Schwarzkopf,E.A., Bradstreet,T.R., Gygi,S.P., Edelson,B.T. and
            Mosammaparast,N.
  TITLE     OTUD4 Is a Phospho-Activated K63 Deubiquitinase that Regulates
            MyD88-Dependent Signaling
  JOURNAL   Mol Cell 69 (3), 505-516 (2018)
   PUBMED   29395066
REFERENCE   7  (bases 1 to 7327)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 7327)
  AUTHORS   Dey,A., Seshasayee,D., Noubade,R., French,D.M., Liu,J.,
            Chaurushiya,M.S., Kirkpatrick,D.S., Pham,V.C., Lill,J.R.,
            Bakalarski,C.E., Wu,J., Phu,L., Katavolos,P., LaFave,L.M.,
            Abdel-Wahab,O., Modrusan,Z., Seshagiri,S., Dong,K., Lin,Z.,
            Balazs,M., Suriben,R., Newton,K., Hymowitz,S., Garcia-Manero,G.,
            Martin,F., Levine,R.L. and Dixit,V.M.
  TITLE     Loss of the tumor suppressor BAP1 causes myeloid transformation
  JOURNAL   Science 337 (6101), 1541-1546 (2012)
   PUBMED   22878500
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AW494258.1, BC138373.1 and AC101687.11.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC138373.1, SRR11927934.7589618.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                AW494258.1         1-28
            29-6856             BC138373.1         1-6828
            6857-7327           AC101687.11        173517-173987
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 37.74 cM"
     gene            1..7327
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /note="OTU domain containing 4"
                     /db_xref="GeneID:73945"
                     /db_xref="MGI:MGI:1098801"
     exon            1..389
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..3554
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1; OTU
                     domain-containing protein 4"
                     /codon_start=1
                     /product="OTU domain-containing protein 4 isoform 1"
                     /protein_id="NP_001242962.1"
                     /db_xref="CCDS:CCDS57625.1"
                     /db_xref="GeneID:73945"
                     /db_xref="MGI:MGI:1098801"
                     /translation="MEAAVGAPDGVDQGGVGPLEDETPMDAYLRKLGLYRKLVAKDGS
                     CLFRAVAEQVLHSQSRHVEVRMACIRYLRENREKFEAFIEGSFEEYLKRLENPQEWVG
                     QVEISALSLMYRKDFVIYQEPNVSPSHVTENNFPEKVLLCFSNGNHYDIVYPITYKDS
                     SAMCQSLLYELLYEKVFKTDVSKIMMGLEASEVAEESNSEISDSEDDSCKSKSTAATD
                     VNGFKPSGSENPKNNGNSADLPLSRKVLKSLNPAVYRNVEYEIWLKSKQAQQKRDYSI
                     AAGLQYEVGDKCHQVRLDHNGKLSNADIHGVHSENGLVLSEELGKKHTPKNLKPPPPE
                     SWNTVSGKKMKKPNSGQNFHSDTDYRGPKNLNKPIKAPSALPPRLQHPSSGVRQHAFS
                     SHSTGSQSQKSSSEHKNLSRMPSQITRKPDRERAEDFDHVSRESYYFGLSPEERREKQ
                     AIEESRLLYEIQNRDEQAFPALSSSSVSQSPSQNSNACVPRKSSHARDRKGSMRRADA
                     EERKDKDSLRGHTHVDKKPEPSTLEISDDKCTRVSSPSKSKKECPSPVEQKPAEHIPL
                     SNPAPLLVSPEVHLTPAVPSLPATVPAWPSEPTTFGPTGVPAQIPILSVTQTTGPDAA
                     VSQAHLTPSPVPVSIQAVNQPLMPLPQTMSLYQDPLYPGFPCSEKGDRAIAPPYSLCQ
                     TGEDLPKDKNILRFFFNLGVKAYSCPMWAPHSYLYPLHQAYMAACRMYPKVPVPVYPQ
                     NTWFQEAPPAQSESDCPCTDAHYSLHPEASVNGQMPQAEMGPPAFASPLVIPPSQVSE
                     GHGQLSYQPELESENPGQLLHAEYEESLSGKNMYPQQSFGPNPFLGPVPIAPPFFPHV
                     WYGYPFQGFVENPVMRQNIVLPPDDKGELDLPLENLDLSKECDSVSAVDEFPDARVEG
                     AHSLSAASVSSKHEGRVEQSSQTRKADIDLASGSSAVEGKGHPPTQILNREREPGSAE
                     PEPKRTIQSLKEKPEKVKDPKTAADVVSPGANSVDRLQRPKEESSEDENEVSNILRSG
                     RSKQFYNQTYGSRKYKSDWGSSGRGGYQHVRGEESWKGQPNRSRDEGYQYHRHVRGRP
                     YRGDRRRSGMGDGHRGQHT"
     exon            390..473
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            474..524
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            525..571
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            572..644
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            645..726
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            727..859
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            860..914
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1032
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1097
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1195
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1296
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1486
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1649
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1773
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1829
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1904
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2043
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2298
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2339
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     exon            2340..7327
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7223..7228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /note="hexamer: ATTAAA"
     polyA_site      7241
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /note="major polyA site"
     regulatory      7309..7314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /note="hexamer: AGTAAA"
     polyA_site      7327
                     /gene="Otud4"
                     /gene_synonym="4930431L18Rik; D8Ertd69e; mKIAA1046"
                     /note="major polyA site"
ORIGIN      
        1 cggccgcgtg cggatggcca tgcactaggc tgggctcacc tatgctccgg gagagcgccg
       61 gccaccgcct cgggccgctg ccgccaccgc ctcttcctgc cgccgctcgc cgtgtccgcg
      121 tcggagccgg agcccgagcc gcagcctgcc ccaggcccgg gcgtcgagca gccggcggcg
      181 cggccatgtg gggctagttc gccccgcgca cgctgcagcg gaccggcaac atggaggcag
      241 ccgtcggcgc gcccgacggc gtggaccagg gcggcgtggg gccgctggag gatgagacgc
      301 ccatggacgc ctatctgcgc aaactgggct tgtatcggaa attggtcgcc aaggacgggt
      361 cgtgcttgtt ccgggctgtg gccgagcagg tgttgcactc tcagtctcgg catgtggagg
      421 ttaggatggc ctgtatccgc taccttcgag agaacagaga gaaatttgaa gcgtttatag
      481 aagggtcatt tgaagaatat ttaaaacgtt tggaaaatcc acaggaatgg gtaggacaag
      541 tggaaataag tgccctttca cttatgtaca ggaaagattt tgtaatatat caggagccaa
      601 atgtttctcc ttcacatgta actgaaaata attttcctga gaaggtgtta ctgtgttttt
      661 caaatggaaa tcattatgac attgtctatc ccataacata taaagatagt tctgctatgt
      721 gtcagtctct cctttatgag ttgctgtatg agaaggtatt caaaactgat gttagtaaaa
      781 tcatgatggg actagaagcc tctgaggtgg ctgaggagag caacagtgag atatcagact
      841 ctgaggacga cagctgcaag agtaaaagta ctgctgctac tgatgtgaat ggatttaaac
      901 cctcaggcag tgagaaccct aagaacaatg ggaactcagc tgaccttcct ttgtccagaa
      961 aggttcttaa gtcactcaac ccagcagtct atagaaatgt ggagtatgaa atttggttga
     1021 agtctaaaca agctcaacaa aaacgtgatt attccattgc tgctggctta cagtatgaag
     1081 ttggagataa atgccaccag gttagattgg atcataatgg gaaattatct aatgcagaca
     1141 ttcatggggt tcactctgag aatggactgg ttttgtctga agaactgggg aaaaaacata
     1201 caccgaagaa cctcaagcca cctcccccag aaagctggaa cacggtgtca ggaaagaaga
     1261 tgaaaaaacc taattctggg caaaatttcc attcagatac agattacaga gggccaaaga
     1321 atctaaacaa gccaatcaaa gccccatctg cactacctcc tcgactccag catccttcat
     1381 cgggtgtaag acagcatgca ttctccagtc attctacagg gtcccagtct cagaaatcct
     1441 ccagtgagca taagaatcta agtaggatgc cctcacagat cacaagaaaa cctgaccgtg
     1501 aaagggctga ggactttgat cacgtgagtc gtgaatctta ctattttggc ctctccccag
     1561 aagaacgcag agagaagcaa gctattgaag agtctcgttt actgtatgag attcagaacc
     1621 gggatgaaca ggctttccct gccctttcta gttcatcagt cagtcagtca ccttctcaga
     1681 atagcaatgc gtgtgtccca aggaagtctt cacatgcaag ggacaggaaa ggaagcatgc
     1741 ggagagcaga cgcagaggaa cgaaaggaca aagactctct acgtggccat actcatgtgg
     1801 ataaaaaacc ggagccaagc acactggaga tcagcgatga taaatgtaca agagtttcat
     1861 caccatctaa gtcaaagaaa gagtgcccat ctcctgtaga acaaaagcca gcagaacata
     1921 tacctttgtc aaatccagct ccccttctag tttctccaga agtacatctc actcctgcgg
     1981 tgccttcttt accagccact gtgccagcct ggccaagtga acctacaact ttcggaccaa
     2041 caggtgtccc tgctcagatt cccattttgt cagtgacaca gaccactgga cctgatgctg
     2101 ccgtgtcaca agcgcattta acaccttctc cggttcctgt gtcaattcag gcagttaacc
     2161 agcccttgat gcctttgcct cagacaatga gcctctatca agaccccctc tatcctgggt
     2221 ttccttgtag tgagaaggga gatcgagcca ttgcaccacc ttattcactg tgtcagaccg
     2281 gggaggacct gcctaaagat aagaatattc ttcgattctt cttcaatctc ggtgtaaagg
     2341 catatagttg tcctatgtgg gccccacatt cttacctata tcctctgcac caggcctata
     2401 tggcagcctg caggatgtac ccaaaggtcc ctgttcccgt gtatcctcag aatacttggt
     2461 tccaagaagc ccctcctgct cagagtgaaa gtgactgtcc ttgcaccgat gcccactact
     2521 ctctgcaccc cgaggccagt gttaatggtc agatgccaca ggcagagatg ggaccgcctg
     2581 catttgcatc acctctggtt atccctccat ctcaggtgtc tgaaggtcat ggacaattgt
     2641 cttaccaacc tgaactggag tctgagaacc cagggcagct tcttcatgct gaatatgaag
     2701 agtcacttag tggcaagaac atgtacccac aacagtcttt tgggcctaac ccatttttag
     2761 gtcctgttcc cattgcacct cctttcttcc ctcatgtttg gtatgggtat ccttttcagg
     2821 gattcgtaga aaatcctgta atgaggcaaa atattgtcct gccccctgat gataaaggag
     2881 aattggattt gcctttggag aatctagatc tgtctaaaga atgcgattct gtctcagcag
     2941 tagatgagtt cccagacgcc agagttgaag gtgcacattc tctgtctgca gcgagtgtga
     3001 gcagcaagca cgaaggccga gtggagcagt cgtcccagac ccggaaggca gacatagact
     3061 tggcttcagg ttcttctgca gtggaaggaa agggtcatcc tcccactcag attctaaaca
     3121 gagaaagaga acctgggtct gctgaacctg agcctaagag gaccattcaa agtctgaaag
     3181 aaaaaccaga gaaagtaaaa gatcccaaga ctgctgctga tgtggtcagc cctggggcca
     3241 attctgtgga tagattgcaa agaccaaaag aagagagttc agaagatgag aatgaagtat
     3301 ctaatatttt gagaagtggc agatccaagc agttttataa tcaaacttac ggaagcagga
     3361 agtacaaaag tgattggggc tcttctggtc gaggtggcta tcaacacgtg agaggcgagg
     3421 agtcctggaa agggcagcca aatcgaagcc gggatgaagg ttatcagtac catcgacatg
     3481 ttagaggacg cccatacagg ggagatagga ggagatcagg gatgggagat ggccacaggg
     3541 gacaacacac ttaatggtta attattgctc atgttgtaga gccttttctt aggtggagta
     3601 tttctgaagg ctttaaaatc attacaataa aaacccaaat tggaactatc tcctcccttt
     3661 acccctttcc cttaattcct attctgatga cactttggac atgaattcaa ggggcagatg
     3721 aatttttggc attgtgtttt ttcttactca aaatctgttt attgggttac tgcccccata
     3781 aaaagtaaac atgactttta agtatttttt tataaacagc tcaatataaa acatagacag
     3841 tgtttgatta ttttccttgt gtaagtttga tttaaaacgt tggaaatgtg tgctttttag
     3901 tgtttactaa agtgataaga aaataaagca ttcaatacac tatagattcc aaaacataac
     3961 attgcaccaa atagaaatgt atattttatt atgcaatgcc ttagtcataa actgggctca
     4021 aacaatcctc agcctaaaac actgttgtct tttaatatgc ttccaaccca aaggcctttc
     4081 atctcagtat ctgtcaaact tgaataacgt cttctcttta ctattacaca cgaggcagcc
     4141 tattaacctg tgtcttagaa ttgttgtata tagttctttt aatatccata gggtatattt
     4201 ttgaattttt tggtgagtat ttgttgaatt tgagatagca tacagtagat gtttaagaaa
     4261 tagtaggaag tccggtgaga cggctgtttc catcaagaac tcctagcact gctgtaaata
     4321 tcatggtgcc tactggaagg gaatgtagat gctatgcatt ttggaaataa tctgcatctg
     4381 ttaaacctgc agaagttttt taatgccact ttaacactaa tgcactgaca gattctaaat
     4441 attttgtgag aaatgttgaa atgtttaacc tgataggctt ctctataaaa gagtgttttg
     4501 tttttttccc tgcaccacaa gctgtgttta tcactttaca gttgcatgtt caacttgtca
     4561 tagctggaat tactgtataa aaagaactga ttgtgacttg tagttcttct ctagaggatg
     4621 ctgctagaac gtggttttgc tttgcatttt gtagttcttc ccgtcagtgc tgtgtgtagt
     4681 cctgcttctt ccagtctgca gtattcacta gaggcgccct ttgcatgttg cactctgttc
     4741 tcatttggag gttggactca gaccagttag cacagtattc tctcatctgt gtcactttgt
     4801 aaaactaact gtactctgta tttcttattt gtacatatca atgtgagaaa tctccctttt
     4861 ttatgttgca attaccttgt gatcaggcag cttgagtgct atgcaaatag taagtagtgt
     4921 agtggtgatt tttctttgca tgttgtgtgt gatataccta gccagaaata gatgtggctt
     4981 ttgttttggg ggcagattac tttcaaaagc aaatacaatt cacttgaatt tgacaaactg
     5041 aagcagacaa gtgttctggg tcctctgata atttggggtg tttggctgtc agctaggctc
     5101 atgaagtcca ctgtactgta atgatggtat ttacctctgt gctattttaa ttaccctcgc
     5161 gtctgtggaa ctgctgattt gagtagtgat tagcatttag aaattttgta atgtagagtt
     5221 ttagagagag cactttgaaa gataaacatt ttattatgat ggtgctaggt acaaaattta
     5281 tagcatggga tgtgaagaaa aaaaatgaga acccattgaa aggaagaaag gaatttgttg
     5341 tctgcttcta agctagagtg gttgtaaagg ttctgctctg ccagtgttca gtatcagtgg
     5401 ctgaattatg gataagaact gtagagaatc ttctgtttag tccgtgcttt ttatgtagaa
     5461 ttggttttat ctaatagttt tactatggaa atcgcctttt gatattaaag ccagatttta
     5521 gaggtttgat atgtttggtc tcaggagctc aaaagaagta gctttttcca gtgtcttttg
     5581 tgttactgat ttgggaaatg ttgaaagatt ggacagggaa gaatagcgct tggtgtcctc
     5641 atggtcattc tgtcttacct tagtggcttg acagtactta ctatagctcc tgagggaagc
     5701 caatcaactt ctgttttcct acctgacctg cagggcatga tggatcagtg atgaaagaat
     5761 tgtagcctgt ggcactttgt tttgacctct ggtatagaac tctgactttt atagttttaa
     5821 aatgatcaat ctttgtatga aagtcagttt tctttcttta ggtatcagaa gattttgcct
     5881 tattctgagg tcggactagg gccaagcaag ctttttcctt catatgagct gtcatactgt
     5941 attttgacct ctttctgcac gaaaaaagaa agaaaaatga cagctggaaa aagtcctttg
     6001 tattggcaca tgataaatct tgatgacggg ctatattctg aacaaacgct tgcactgacc
     6061 aaagcttgtg tttagcgtgt gtttagcata ggtgctgtag ttggagtggg ccccacctct
     6121 gctcctttca gtgttcttgc tgttcaagtt attcctctca gacatcctcc attgtcccac
     6181 gcactccttg gcagcagaat gctggcagct tgtgaccctc atgtttcctg taagtctgtt
     6241 tcatggtagg gttcatacct gcctcagcct gacaggagta ggttttccgc aacccactgt
     6301 gctgagacag ttgatgtgat gcaaactatt cttggagtgt gttgttttct ttggcccaag
     6361 ggtaaactaa aatacctgtg aactgcaaac ttgcttgtga ttgacttggt gcaatacagt
     6421 tcctttctaa acgtttctgg tttagagaat actcagccat cctggaaact agcaatattt
     6481 attttgcctc atttacttta ttttcagttt tagatgaggt gaaggttttc tttcagttag
     6541 ggtacttggc aaggtccatt ctagtgtagc tgaagaggaa gctatactat tttctgttta
     6601 ctgtattttg ctccctattt tatgcttttt aaaaatcttt cacagtgact cacttgattg
     6661 agcatattga tttgaaagag ctaagatttt cctgcttaat gtcagccatt ggctctcctg
     6721 ataattggga ctttttgttt gtttgttttt tgttgttttg ttttttttgt ttcccctttc
     6781 cctaaaatta taagtttaga gaatcctgta agaggggtgg gtgcatgtct taatgctgag
     6841 aagcagcagc tttggggttg aatttacttg aatggtttaa aaagattgca ttgttacaaa
     6901 gctagaaaaa atttatgtat gaaaaatgat cctagcctta cactgagtac aataatactt
     6961 ataagcatgt gaagatgtga tctttgtgat gttacttgta caaacatata tacatatcta
     7021 tacatatctt ttgaagtgtt gaaaggaata gtactttata ttctttatca taccactttt
     7081 gtaagtgttg aatatattgc tgtttatttt tctatgttct gtttcgtagc cttaagaagt
     7141 gtttcaaacg atctgaatgt ataaaacaag tcaatgccct acatggtgtg atgctgcatt
     7201 atatatacaa ccgtgtgcat atattaaatt ctgtttgtcg agtctgcttg ggattctttg
     7261 gtctttgggt aacccaatat atcatatgta aatctgagga tgaatcttag taaactcttg
     7321 aaaatga
//
