LOCUS       NM_001177841            3246 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus OTU domain, ubiquitin aldehyde binding 2 (Otub2),
            transcript variant 1, mRNA.
ACCESSION   NM_001177841
VERSION     NM_001177841.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Chang,W., Luo,Q., Wu,X., Nan,Y., Zhao,P., Zhang,L., Luo,A.,
            Jiao,W., Zhu,Q., Fu,Y. and Liu,Z.
  TITLE     OTUB2 exerts tumor-suppressive roles via STAT1-mediated CALML3
            activation and increased phosphatidylserine synthesis
  JOURNAL   Cell Rep 41 (4), 111561 (2022)
   PUBMED   36288705
REFERENCE   3  (bases 1 to 3246)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3246)
  AUTHORS   Beck,A., Vinik,Y., Shatz-Azoulay,H., Isaac,R., Streim,S., Jona,G.,
            Boura-Halfon,S. and Zick,Y.
  TITLE     Otubain 2 is a novel promoter of beta cell survival as revealed by
            siRNA high-throughput screens of human pancreatic islets
  JOURNAL   Diabetologia 56 (6), 1317-1326 (2013)
   PUBMED   23515685
  REMARK    GeneRIF: siRNAs of OTUB2 impaired insulin secretion both under
            basal conditions and in cytokine-treated MIN6 cells
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160131.2 and AC154347.2.
            
            On Feb 9, 2022 this sequence version replaced NM_001177841.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076336.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               AC160131.2         199493-199691
            200-626             AC154347.2         26218-26644
            627-722             AC154347.2         33917-34012
            723-841             AC154347.2         34670-34788
            842-926             AC154347.2         36538-36622
            927-1121            AC154347.2         36955-37149
            1122-3246           AC154347.2         37947-40071
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 52.92 cM"
     gene            1..3246
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /note="OTU domain, ubiquitin aldehyde binding 2"
                     /db_xref="GeneID:68149"
                     /db_xref="MGI:MGI:1915399"
     exon            1..199
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            200..626
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     CDS             357..1328
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin thioesterase OTUB2; otubain-2; deubiquitinating
                     enzyme OTUB2; ubiquitin-specific-processing protease
                     OTUB2; OTU domain-containing ubiquitin aldehyde-binding
                     protein 2"
                     /codon_start=1
                     /product="ubiquitin thioesterase OTUB2 isoform 1"
                     /protein_id="NP_001171312.1"
                     /db_xref="CCDS:CCDS49150.1"
                     /db_xref="GeneID:68149"
                     /db_xref="MGI:MGI:1915399"
                     /translation="MALPSSLQDLIPRFHLRRHPRGLFCASSCQRSHTESSGKTPTLP
                     SVLRLVLPTPKSPLVACSQLRRLLQHPPGRCPSCSHCPIPKPSCLQSETSFNLISEKC
                     DILSILRDHPENRIYQRKIQELSKRFTSIRKTKGDGNCFYRALGYSYLESLLGKSREI
                     LKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDSSVSSLLKVFNDQSSSD
                     RIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMAMECDHVQITALSQAL
                     NIALQVEYVDEMDTALNHHVFPEAAIPSVYLLYKTSHYNILYAAEKH"
     exon            627..722
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..841
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            842..926
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1121
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..3246
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1466
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /note="major polyA site"
     polyA_site      1622
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
     regulatory      3221..3226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /note="hexamer: ATTAAA"
     polyA_site      3241
                     /gene="Otub2"
                     /gene_synonym="2010015L18Rik; 4930586I02Rik; OTB2; OTU2"
                     /note="major polyA site"
ORIGIN      
        1 aatcgcaggg tgtgcgtctt gctgggacac agtggaggtc tgcgctccgg tctgaggggc
       61 ggtcgggtgt attctgcgct cacccacacc cgcgaggccc gcgccgcccg acccaacggc
      121 ggagccgccc agcgcctccc ctggcacccg ctcgctcctc gctggggcgg gacgaggccc
      181 gacggctccg gtcactatgg tgataaagtt acaagaaccc acttcgcagc ctgaatgccg
      241 ctgccttggg cttctgccac ctaggtcctt cctagctcat tgttcttaag ttctgttttc
      301 ccatggagac ctgaggcaag gacaccctgc agccaagttc tttgccactt gcggagatgg
      361 cccttccctc cagtctccag gacctcattc ctcgcttcca tctgaggcgc catcctcggg
      421 gcctcttttg tgccagttcc tgccagcgct ctcacacaga atcttctggg aagaccccca
      481 ctttaccttc tgttctccgt cttgtcttac ccactccaaa atcgcccttg gtggcctgtt
      541 cacagctgcg aaggctgctc cagcatcctc cagggaggtg tcccagctgt agccactgcc
      601 ccatcccgaa gccttcatgt ctgcagagtg aaacatcttt caacctaata tcagaaaaat
      661 gtgacattct atccattctt cgggatcatc ctgaaaacag gatttaccag aggaaaatcc
      721 aggaactcag caaaagattc acctcgatcc ggaagaccaa aggagacgga aactgcttct
      781 acagggcctt aggctattcc tacctggagt ccttgctggg caagagcaga gagatcctca
      841 agttcaaaga gcgtgtgcta cagaccccaa atgaccttct ggctgccggc tttgaggaac
      901 acaagttcag aaacttcttt aatgcttttt acagtgtggt tgagctggta gagaaggata
      961 gctcagtgtc cagcctgctg aaggtgttca atgaccagag ttcctcggac cgaatcgtgc
     1021 agttcttacg cctcctcacg tcggccttca tcaggaaccg agctgacttc ttccgacatt
     1081 tcattgatga ggagatggac atcaaagact tctgcactca cgaagtagag cccatggcca
     1141 tggagtgtga ccacgtgcag atcacagccc tgtcgcaggc actcaacatt gctctgcagg
     1201 tagagtacgt cgacgagatg gacaccgctc tgaaccacca cgtgttcccc gaggctgcca
     1261 tcccttcggt ttatctgctc tataaaacat cccactacaa catcctttac gcagccgaga
     1321 aacactgact acttttgggc catgccggag cccgtcatcg aatggactgc attctgaatg
     1381 gaacatcctg ccttggcaat ttttttgagt gacttagaca actaggaaac atatagcctc
     1441 ttgggtaaag ccacactgaa ctgagacctg tgcccactat ggactgtgaa tgacttaata
     1501 ggaattttag tatttatttt gatagatgat ataatgcctg ctaactctgg accacaaggc
     1561 cagaaactct tctctctgta cccatttact tcaagaaccc cattggacct atttatctgg
     1621 aagaggttcc tgacagtgct gctatgtgaa aatggggact attgggcttg tcctcagcca
     1681 gggcagtaac catacagggg agaaaagctc gggccagctg tcatgttcag gtgacttacc
     1741 agacctatgc agagtccagc caggctatgt cgggtgggtc atggtagcct gtctaaatgt
     1801 gaggatgtgt gtactttcat tcacggtggc tatacttgcc cgtcccctga agggaagagg
     1861 gaacaaaaac ataaaagact tttcccaaag accagaaaag ggctttagcc ttcagcacaa
     1921 agctcaggtt gtgaggtcgg gaccagaact cctgactctg gaggacaggc cctctagggt
     1981 tctgctgtca atcccacctg gataaggctt gccaaggact agggcttgta cccgagttag
     2041 tgtgtccatg tctgggacca aggggctaca gctgtccatc ctccccagtg aatagggtct
     2101 aggtattcag ctggatatgt gcagttctga cctggaagga ctgaggacag gtttcgcaag
     2161 gctctttggt tttcttcagc ctagcttcat aggcagttag gcagggaagg tcagacaagc
     2221 catagatgtc ggcagcaggg acatttcctc ctctgcacgt gccccttaat agccacttac
     2281 cttgccttgc tgcctcacct gaggacacac ctggcagctg ctagccacac cctcctctgt
     2341 aggataacca tgggaccacc ctgtggtcct tcctttgtta cagggctccc ctcatgcttc
     2401 actctgcagg aattagacta ttggtgctac tgtgagggtc tgctgacaac ctacacacag
     2461 ggctggaagc aggatcctga gtcttacaca attcagattt gtatctgtcc ttttgtttgg
     2521 tgtaagccaa aagcaccaag atgtctgggt agtgtgcagt ttccaagacc cactttggca
     2581 acatttagat ccaaaggctc cagctaagtg agtatcttgc tggcacaggg gggtaaatct
     2641 tgccctttca atgtgttcag tgtcccacgg tgacctgggt atctatggca gttgcccaca
     2701 gcgtgggccc ttatttccac aactgtcttc agccagtagt ctccatttcc aggcttaata
     2761 ttatgagcac aggatgcctg actgatggat agcctgggct gctccagggg catgggctta
     2821 aacacatgct aaaacctggc cgagcatagc agttttctgg ctaggttaca gcaacaagtt
     2881 ctatcagctc tttctagagg aaacagtgga gcgcaggtgc tgagagctca tctttgaatt
     2941 cctcatctcc cagaacagcc agtggggaca gtaacagccc ggtacacctg ctatctgtct
     3001 tctgagaaca gatgcaagtg ctggggccag ctgcccaagt tcaactgtgt gatcttggtc
     3061 aaatcccttc accttggaag caaagcagca tgccaccttg gaagtgaacg ggggtctaag
     3121 agccaagtaa acagccagcc tgggaaagtg gggcttgggg ttctgtgagt acccaaggcc
     3181 aagccctaca gcttccctca agtgaaaaca tttccctaac attaaacgtt tctataaaca
     3241 actgtc
//
