LOCUS       NM_172709               3171 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus otopetrin 1 (Otop1), mRNA.
ACCESSION   NM_172709
VERSION     NM_172709.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Tu,Y.H., Liu,N., Xiao,C., Gavrilova,O. and Reitman,M.L.
  TITLE     Loss of Otopetrin 1 affects thermoregulation during fasting in mice
  JOURNAL   PLoS One 18 (10), e0292610 (2023)
   PUBMED   37812612
  REMARK    GeneRIF: Loss of Otopetrin 1 affects thermoregulation during
            fasting in mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3171)
  AUTHORS   Liang,Z., Wilson,C.E., Teng,B., Kinnamon,S.C. and Liman,E.R.
  TITLE     The proton channel OTOP1 is a sensor for the taste of ammonium
            chloride
  JOURNAL   Nat Commun 14 (1), 6194 (2023)
   PUBMED   37798269
  REMARK    GeneRIF: The proton channel OTOP1 is a sensor for the taste of
            ammonium chloride.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3171)
  AUTHORS   Jin,H., Fishman,Z.H., Ye,M., Wang,L. and Zuker,C.S.
  TITLE     Top-Down Control of Sweet and Bitter Taste in the Mammalian Brain
  JOURNAL   Cell 184 (1), 257-271 (2021)
   PUBMED   33417862
REFERENCE   5  (bases 1 to 3171)
  AUTHORS   Teng,B., Wilson,C.E., Tu,Y.H., Joshi,N.R., Kinnamon,S.C. and
            Liman,E.R.
  TITLE     Cellular and Neural Responses to Sour Stimuli Require the Proton
            Channel Otop1
  JOURNAL   Curr Biol 29 (21), 3647-3656 (2019)
   PUBMED   31543453
  REMARK    GeneRIF: Cellular and Neural Responses to Sour Stimuli Require the
            Proton Channel Otop1.
            GeneRIF: The proton channel OTOP1 forms a sour receptor in mice
REFERENCE   6  (bases 1 to 3171)
  AUTHORS   Tu,Y.H., Cooper,A.J., Teng,B., Chang,R.B., Artiga,D.J.,
            Turner,H.N., Mulhall,E.M., Ye,W., Smith,A.D. and Liman,E.R.
  TITLE     An evolutionarily conserved gene family encodes proton-selective
            ion channels
  JOURNAL   Science 359 (6379), 1047-1050 (2018)
   PUBMED   29371428
  REMARK    GeneRIF: Otop1 and other members of the Otopetrin gene family
            encode proton-selective ion channels. Otop1 is expressed in taste
            bud cells that detect sour taste and is required for the proton
            current in these cells.
REFERENCE   7  (bases 1 to 3171)
  AUTHORS   Smith,M., Yuan Wang,X., Wolgemuth,D.J. and Murashov,A.K.
  TITLE     Development of the mouse vestibular system in the absence of
            gravity perception
  JOURNAL   Brain Res Dev Brain Res 140 (1), 133-135 (2003)
   PUBMED   12524184
REFERENCE   8  (bases 1 to 3171)
  AUTHORS   Hurle,B., Lane,K., Kenney,J., Tarantino,L.M., Bucan,M.,
            Brownstein,B.H. and Ornitz,D.M.
  TITLE     Physical mapping of the mouse tilted locus identifies an
            association between human deafness loci DFNA6/14 and vestibular
            system development
  JOURNAL   Genomics 77 (3), 189-199 (2001)
   PUBMED   11597144
REFERENCE   9  (bases 1 to 3171)
  AUTHORS   Ying,H.C., Hurle,B., Wang,Y., Bohne,B.A., Wuerffel,M.K. and
            Ornitz,D.M.
  TITLE     High-resolution mapping of tlt, a mouse mutant lacking otoconia
  JOURNAL   Mamm Genome 10 (6), 544-548 (1999)
   PUBMED   10341082
REFERENCE   10 (bases 1 to 3171)
  AUTHORS   Ornitz,D.M., Bohne,B.A., Thalmann,I., Harding,G.W. and Thalmann,R.
  TITLE     Otoconial agenesis in tilted mutant mice
  JOURNAL   Hear Res 122 (1-2), 60-70 (1998)
   PUBMED   9714575
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF548337.1, AK040792.1 and EH101457.1.
            
            On Apr 13, 2007 this sequence version replaced NM_172709.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF548337.1, AK040792.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2534              AF548337.1         1-2534
            2535-3113           AK040792.1         2522-3100
            3114-3171           EH101457.1         403-460
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 20.35 cM"
     gene            1..3171
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /note="otopetrin 1"
                     /db_xref="GeneID:21906"
                     /db_xref="MGI:MGI:2388363"
     exon            1..421
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1827
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /note="tilted"
                     /codon_start=1
                     /product="proton channel OTOP1"
                     /protein_id="NP_766297.2"
                     /db_xref="GeneID:21906"
                     /db_xref="MGI:MGI:2388363"
                     /translation="MPGGPGAPSSPAASSGSSRAAPSGIAACPLSPPPLARGSPQASG
                     PRRGASVPQKLAETLSSQYGLNVFVAGLLFLLAWAVHATGVGKSDLLCVLTALMLLQL
                     LWMLWYVGRSYMQRRLIRPKDTHAGARWLRGSITLFAFITVVLGCLKVAYFIGFSECL
                     SATEGVFPVTHAVHTLLQVYFLWGHAKDIIMSFKTLERFGVIHSVFTNLLLWANSVLN
                     ESKHQLNEHKERLITLGFGNITIVLDDHTPQCNCTPPALCSALSHGIYYLYPFNIEYQ
                     ILASTMLYVLWKNIGRRVDSSQHQKMQCRFDGVLVGSVLGLTVLAATIAVVVVYMIHI
                     GRSKSKSESALIMFYLYAITVLLLMGAAGLVGSWIYRVDEKSLDESKNPARKLDVDLL
                     VATGSGSWLLSWGSILAIACAETRPPYTWYNLPYSVLVIVEKYVQNIFIIESVHLEPE
                     GVPEDVRTLRVVTVCSSEAAALAASTLGSQGMAQDGSPAVNGNLCLQQRCGKEDQESG
                     WEGATGTTRCLDFLQGGMKRRLLRNITAFLFLCNISLWIPPAFGCRPEYDNGLEEIVF
                     GFEPWIIVVNLAMPFSIFYRMHAAAALFEVYCKI"
     exon            422..558
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /inference="alignment:Splign:2.1.0"
     exon            559..617
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /inference="alignment:Splign:2.1.0"
     exon            618..748
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /inference="alignment:Splign:2.1.0"
     exon            749..1656
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..3150
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2415..2420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /note="hexamer: AATAAA"
     polyA_site      2443
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /note="major polyA site"
     regulatory      3130..3135
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /note="hexamer: AATAAA"
     polyA_site      3150
                     /gene="Otop1"
                     /gene_synonym="A530025J20Rik; Otp1; tlt"
                     /note="major polyA site"
ORIGIN      
        1 gccgcccggg gaccagactg gaagatgcct gggggcccgg gcgcgccctc ttctcccgca
       61 gcatcctcag gctcctcgcg tgccgcgccg tcggggatcg cggcatgccc tctgtcccca
      121 cctcccctag cccgagggtc tccacaagct tccggtcccc ggcgcggcgc cagcgtcccg
      181 cagaagctgg cggagactct gagcagccag tatggactga acgtgttcgt ggcggggctg
      241 ctgttcctgc tggcctgggc ggtgcacgcg acgggtgtgg gcaagagcga cctgctgtgc
      301 gttctaaccg cgcttatgct gctgcagctg ctctggatgc tgtggtacgt gggtcgcagc
      361 tacatgcagc gccgcctcat ccgccccaag gacacgcacg cgggtgcgcg ctggcttcgc
      421 ggaagcatca cgttgttcgc gtttatcact gtcgtcctgg gatgcttgaa agtcgcatac
      481 ttcattggat tctcggagtg cttgtcagcc accgagggag ttttcccagt cacccatgca
      541 gtgcataccc tattgcaggt gtatttcctc tggggccatg ctaaggatat catcatgtct
      601 ttcaaaacac tggaaaggtt tggggtgatc cattcagtgt tcacgaacct cctactgtgg
      661 gccaacagcg tcctgaatga atcaaagcac cagctgaatg agcacaagga acggctgatc
      721 actctgggct ttggcaacat caccatcgtt ttggatgacc acacaccaca gtgtaactgc
      781 acaccacccg ccctctgctc tgccctctcc catgggattt actatctgta ccccttcaac
      841 attgagtacc agatcctggc ctcgaccatg ctctacgtgc tgtggaagaa catcgggcgc
      901 agagtggaca gctcccagca ccagaagatg cagtgcagat tcgacggggt cctagtgggc
      961 tccgtgctgg gcttgacagt gctggctgcc accatcgccg tggttgtggt gtacatgatc
     1021 cacatcgggc gctccaaatc caagagcgag tcggctctca tcatgttcta tttgtacgct
     1081 atcacggtgc tgctgcttat gggggccgca gggctagtcg gaagctggat ttacagggtg
     1141 gatgagaagt ctctggatga gtcaaagaac ccagcgcgca agctggatgt tgacctgttg
     1201 gtggccaccg gctccggctc ctggctcctc tcctggggct ccattctggc catcgcctgt
     1261 gctgagactc gcccaccgta cacctggtac aacctgccct actcggtcct ggtgatcgtg
     1321 gagaagtatg tccaaaacat tttcatcatc gagtccgtgc acctcgagcc tgagggggtc
     1381 ccggaggatg tgcgcactct gcgtgtggtc accgtctgca gcagcgaggc tgccgcactg
     1441 gctgcatcca ctctcgggag ccaggggatg gcccaggatg ggtcacctgc tgtcaatgga
     1501 aatctgtgtc tgcagcagag gtgtgggaaa gaggaccagg agtctggctg ggaaggagct
     1561 acggggacaa cccgatgtct ggacttcctt cagggcggca tgaagaggag gcttctcaga
     1621 aacatcacgg cctttctgtt tctttgcaac atctcgcttt ggattccccc tgcctttggc
     1681 tgccgtcccg agtatgacaa cggattggag gaaattgtct ttggctttga accctggata
     1741 attgtggtca acctggccat gcccttttcc attttctacc ggatgcacgc agctgctgcc
     1801 ctctttgagg tctattgtaa gatctagcct gagtcctcat gaaaggagaa gggacggggg
     1861 agcaaggggg ttctgcagcc acctgcgaag ggcggggatg agcaaacact gtctgacaaa
     1921 ggcgggaagg atgccttttg ttctgactgt ctggagttgc cctcgacctg ggggagagcg
     1981 agcagttaaa tcaactgcac aagatagctg gagcctgtcc ttagccaatt ccaatcagat
     2041 ccaaaatgag ttgagcattt tctggctgag accctgtgtt ttagaagaag gtgatccagg
     2101 tttggatgaa aatgatggag tttttcataa caagcatttc ctgtctgtaa tttgtatgac
     2161 tgtgtacaac ttttttctgg ccatctgtgt atcaatccat ttacattttt tttttttcct
     2221 gctcagggtt actgggtttg gaaacaaatg cattttagtt tgtagtttaa tatttgagct
     2281 ggtttaattt tggataaaaa ttagttttga tcaactgcat gagagtctga ttctagactt
     2341 gttctacaaa cacaagacct tccctaaatt tttggtccat ttttatttgt gttcattatg
     2401 aaatattaaa agaaaataaa cattgatgtt ctcttttttg ctacatctat atccagttcc
     2461 tgacgtagaa aatctgaata tttttgggag gagagacagg aaaaatggtc catgttgcac
     2521 tgctaacagt tactctgaac accatggtgt agctttcagc tgtggtgccg tgcagacttt
     2581 tctatattgt tgtacttgct agtgataacc tgacatttct aatgttctcc tcatgctttc
     2641 ctatttgaca gtctgatgac acctttctct acactgtcat aaattcctgg caaacatcat
     2701 tttactgctg tcagaatctc aaactaatgt accacaatta acctcctttg cctttttttg
     2761 attttagaag gctcacaatg ttgctttcct aagtggctct gcgaaagcca agcgtgtttc
     2821 taaaacattt ctcttattta aaaattgcat tttaaaagag acgattggcc ctacatatcc
     2881 attgatccca catctacatt cagccaattt tgcatagaaa atgttttgta agagttgggt
     2941 cttggggttg gagagatggc ctgctgctct caaaaaggac cagagtttgg tttttagtat
     3001 ggcatcagtt ggtttacaac tgcctgcaat tctagctcca gaagatctga tattcttctc
     3061 aggtttccag ggacaccagc actcacatgc aagagtgtgc tacccccatg aataataaaa
     3121 aataataata ataaagcttt caaaaaggaa aaaaaaaaaa aaaaaaaaaa a
//
