LOCUS       NM_001286421            7065 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus otoferlin (Otof), transcript variant 3, mRNA.
ACCESSION   NM_001286421
VERSION     NM_001286421.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Sun,Y., Ren,M., Zhang,Y., Li,S., Luo,Z., Sun,S., He,S., Wang,G.,
            Zhang,D., Mansour,S.L., Song,L. and Liu,Z.
  TITLE     Casz1 is required for both inner hair cell fate stabilization and
            outer hair cell survival
  JOURNAL   Science, eado4930 (2025) In press
   PUBMED   39883789
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   O'Connor,A.P., Amariutei,A.E., Zanella,A., Hool,S.A., Carlton,A.J.,
            Kong,F., Saenz-Roldan,M., Jeng,J.Y., Lecomte,M.J., Johnson,S.L.,
            Safieddine,S. and Marcotti,W.
  TITLE     In vivo AAV9-Myo7a gene rescue restores hearing and cholinergic
            efferent innervation in inner hair cells
  JOURNAL   JCI Insight 9 (23), e182138 (2024)
   PUBMED   39641274
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7065)
  AUTHORS   de Haan,S., Corbat,A.A., Cederroth,C.R., Autrum,L.G., Hankeova,S.,
            Driver,E.C., Canlon,B., Kelley,M.W. and Andersson,E.R.
  TITLE     Jag1 represses Notch activation in lateral supporting cells and
            inhibits an outer hair cell fate in the medial cochlea
  JOURNAL   Development 151 (21) (2024)
   PUBMED   39373109
REFERENCE   4  (bases 1 to 7065)
  AUTHORS   Conrad,L.J., Grandi,F.C., Carlton,A.J., Jeng,J.Y., de Tomasi,L.,
            Zarecki,P., Marcotti,W., Johnson,S.L. and Mustapha,M.
  TITLE     The upregulation of K+ and HCN channels in developing spiral
            ganglion neurons is mediated by cochlear inner hair cells
  JOURNAL   J Physiol 602 (20), 5329-5351 (2024)
   PUBMED   39324853
REFERENCE   5  (bases 1 to 7065)
  AUTHORS   Sellon,J.B., So,K.S., D'Arcangelo,A., Cancelarich,S.,
            Drummond,M.C., Slade,P.G., Pan,N., Gibson,T.M., Yang,T.,
            Burns,J.C., Palermo,A.T. and Becker,L.
  TITLE     Recovery kinetics of dual AAV-mediated human otoferlin expression
  JOURNAL   Front Mol Neurosci 17, 1376128 (2024)
   PUBMED   38952419
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7065)
  AUTHORS   Schwander,M., Sczaniecka,A., Grillet,N., Bailey,J.S., Avenarius,M.,
            Najmabadi,H., Steffy,B.M., Federe,G.C., Lagler,E.A., Banan,R.,
            Hice,R., Grabowski-Boase,L., Keithley,E.M., Ryan,A.F.,
            Housley,G.D., Wiltshire,T., Smith,R.J., Tarantino,L.M. and
            Muller,U.
  TITLE     A forward genetics screen in mice identifies recessive deafness
            traits and reveals that pejvakin is essential for outer hair cell
            function
  JOURNAL   J Neurosci 27 (9), 2163-2175 (2007)
   PUBMED   17329413
REFERENCE   7  (bases 1 to 7065)
  AUTHORS   Roux,I., Safieddine,S., Nouvian,R., Grati,M., Simmler,M.C.,
            Bahloul,A., Perfettini,I., Le Gall,M., Rostaing,P., Hamard,G.,
            Triller,A., Avan,P., Moser,T. and Petit,C.
  TITLE     Otoferlin, defective in a human deafness form, is essential for
            exocytosis at the auditory ribbon synapse
  JOURNAL   Cell 127 (2), 277-289 (2006)
   PUBMED   17055430
  REMARK    GeneRIF: Otoferlin is essential for a late step of synaptic vesicle
            exocytosis and may act as the major Ca(2+) sensor triggering
            membrane fusion at the auditory inner hair cell ribbon synapse.
REFERENCE   8  (bases 1 to 7065)
  AUTHORS   Wilson,L., Ching,Y.H., Farias,M., Hartford,S.A., Howell,G.,
            Shao,H., Bucan,M. and Schimenti,J.C.
  TITLE     Random mutagenesis of proximal mouse chromosome 5 uncovers
            predominantly embryonic lethal mutations
  JOURNAL   Genome Res 15 (8), 1095-1105 (2005)
   PUBMED   16024820
REFERENCE   9  (bases 1 to 7065)
  AUTHORS   Yasunaga,S., Grati,M., Chardenoux,S., Smith,T.N., Friedman,T.B.,
            Lalwani,A.K., Wilcox,E.R. and Petit,C.
  TITLE     OTOF encodes multiple long and short isoforms: genetic evidence
            that the long ones underlie recessive deafness DFNB9
  JOURNAL   Am J Hum Genet 67 (3), 591-600 (2000)
   PUBMED   10903124
REFERENCE   10 (bases 1 to 7065)
  AUTHORS   Yasunaga,S., Grati,M., Cohen-Salmon,M., El-Amraoui,A., Mustapha,M.,
            Salem,N., El-Zir,E., Loiselet,J. and Petit,C.
  TITLE     A mutation in OTOF, encoding otoferlin, a FER-1-like protein,
            causes DFNB9, a nonsyndromic form of deafness
  JOURNAL   Nat Genet 21 (4), 363-369 (1999)
   PUBMED   10192385
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046460.1, AK147256.1 and AK033317.1.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 2. It
            encodes isoform 3, which is shorter than isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150702.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                AK046460.1         3-20
            19-2807             AK147256.1         2-2790
            2808-2997           AK147256.1         3091-3280
            2998-5887           AK033317.1         6-2895
            5888-7063           AK147256.1         6227-7402
            7064-7065           AK033317.1         3869-3870
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 16.48 cM"
     gene            1..7065
                     /gene="Otof"
                     /note="otoferlin"
                     /db_xref="GeneID:83762"
                     /db_xref="MGI:MGI:1891247"
     exon            1..213
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..6068
                     /gene="Otof"
                     /note="isoform 3 is encoded by transcript variant 3;
                     fer-1-like protein 2; protein pachanga"
                     /codon_start=1
                     /product="otoferlin isoform 3"
                     /protein_id="NP_001273350.1"
                     /db_xref="GeneID:83762"
                     /db_xref="MGI:MGI:1891247"
                     /translation="MALIVHLKTVSELRGKGDRIAKVTFRGQSFYSRVLENCEGVADF
                     DETFRWPVASSIDRNEVLEIQIFNYSKVFSNKLIGTFCMVLQKVVEENRVEVTDTLMD
                     DSNAIIKTSLSMEVRYQATDGTVGPWDDGDFLGDESLQEEKDSQETDGLLPGSRPSTR
                     ISGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIQLGDGLDPD
                     SVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCV
                     EVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVG
                     SFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKAN
                     ETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDL
                     VDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVA
                     IGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRA
                     RLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRK
                     NGDKPITFEVTIGNYGNEVDGMSRPLRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDLA
                     SVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEG
                     LNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGRSSRTRLDRERLKSCM
                     RELESMGQQAKSLRAQVKRHTVRDKLRSCQNFLQKLRFLADEPQHSIPDVFIWMMSNN
                     KRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLW
                     LGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLF
                     AADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPI
                     IVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGSATAGDLL
                     AAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKR
                     VNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIR
                     VVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTTGEVVVSMEPEEPVKKLETM
                     VKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEPEEEEPDESMLDWWSKYFASIDTMK
                     EQLRQHETSGTDLEEKEEMESAEGLKGPMKSKEKSRAAKEEKKKKNQSPGPGQGSEAP
                     EKKKAKIDELKVYPKELESEFDSFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGS
                     LCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKAD
                     PYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDL
                     IGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHF
                     GPHGRVRVANRVFTGPSEIEDENGQRKPTDEHVALSALRHWEDIPRVGCRLVPEHVET
                     RPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVL
                     EDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEE
                     KIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAK
                     QCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAE
                     KNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKFLLLFLLLLL
                     FALFLYSLPGYLAKKILGA"
     exon            214..272
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            273..361
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            362..461
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            462..640
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            641..714
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            715..841
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            842..896
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1028
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1091
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1176
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1336
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1523
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1710
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1934
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2043
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2224
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2225..2345
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2446
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2537
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2654
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2807
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2997
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3122
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3123..3257
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3258..3419
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3539
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3540..3701
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3702..3864
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..3935
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3936..3965
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            3966..4097
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4098..4164
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4165..4301
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4302..4436
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4574
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4575..4702
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4873
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            4874..5034
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5035..5177
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5178..5266
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5267..5365
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5366..5607
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5608..5786
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5787..5887
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            5888..6090
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     exon            6091..7065
                     /gene="Otof"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7046..7051
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Otof"
                     /note="hexamer: AATAAA"
     polyA_site      7062
                     /gene="Otof"
                     /note="major polyA site"
ORIGIN      
        1 ttggttgcct tggtctctgt gggcagcagc aggaggaggc ggcagcagcc agagaagagg
       61 gaggcgtgtg agccacactc caccagcgag cttcttcccg ctgctctgga actgcccagg
      121 ctctccccac cagcatggcc ctgattgttc acctcaagac tgtctcagag ctccgaggca
      181 aaggtgaccg gattgccaaa gtcactttcc gagggcagtc tttctactcc cgggtcctgg
      241 agaactgcga gggtgtggct gactttgatg agacgttccg gtggccagtg gccagcagca
      301 tcgaccggaa tgaagtgttg gagattcaga ttttcaacta cagcaaagtc ttcagcaaca
      361 agctgatagg gaccttctgc atggtgctgc agaaagtggt ggaggagaat cgggtagagg
      421 tgaccgacac gctgatggat gacagcaatg ctatcatcaa gaccagcctg agcatggagg
      481 tccggtatca ggccacagat ggcactgtgg gcccctggga tgatggagac ttcctgggag
      541 atgaatccct ccaggaggag aaggacagcc aggagacaga tgggctgcta cctggttccc
      601 gacccagcac ccggatatct ggcgagaaga gctttcgcag agcgggaagg agtgtgttct
      661 cggccatgaa actcggcaaa actcggtccc acaaagagga gccccaaaga caagatgagc
      721 cagcagtgct ggagatggag gacctggacc acctagccat tcagctgggg gatgggctgg
      781 atcctgactc cgtgtctcta gcctcggtca ccgctctcac cagcaatgtc tccaacaaac
      841 ggtctaagcc agatattaag atggagccca gtgctggaag gcccatggat taccaggtca
      901 gcatcacagt gattgaggct cggcagctgg tgggcttgaa catggaccct gtggtgtgtg
      961 tggaggtggg tgatgacaag aaatacacgt caatgaagga gtccacaaac tgcccttact
     1021 acaacgagta ctttgtcttc gacttccatg tctctcctga tgtcatgttt gacaagatca
     1081 tcaagatctc ggttatccat tctaagaacc tgcttcggag cggcaccctg gtgggttcct
     1141 tcaaaatgga tgtggggact gtgtattccc agcctgaaca ccagttccat cacaaatggg
     1201 ccatcctgtc agaccccgat gacatctctg ctgggttgaa gggttatgta aagtgtgatg
     1261 tcgctgtggt gggcaaggga gacaacatca agacacccca caaggccaac gagacggatg
     1321 aggacgacat tgaagggaac ttgctgctcc ccgagggcgt gccccccgaa cggcagtggg
     1381 cacggttcta tgtgaaaatt taccgagcag agggactgcc ccggatgaac acaagcctca
     1441 tggccaacgt gaagaaggcg ttcatcggtg agaacaagga cctcgtcgac ccctatgtgc
     1501 aagtcttctt tgctggacaa aagggcaaaa catcagtgca gaagagcagc tatgagccgc
     1561 tatggaatga gcaggtcgtc ttcacagact tgttcccccc actctgcaaa cgcatgaagg
     1621 tgcagatccg ggactctgac aaggtcaatg atgtggccat cggcacccac ttcatcgacc
     1681 tgcgcaagat ttccaacgat ggagacaaag gcttcctgcc taccctcggt ccagcctggg
     1741 tgaacatgta cggctccacg cgcaactaca cactgctgga cgagcaccag gacttgaatg
     1801 aaggcctggg ggagggtgtg tccttccggg cccgcctcat gttgggacta gctgtggaga
     1861 tcctggacac ctccaaccca gagctcacca gctccacgga ggtgcaggtg gagcaggcca
     1921 cgcctgtctc ggagagctgc acagggagaa tggaagaatt ttttctattt ggagccttct
     1981 tggaagcctc aatgattgac cggaaaaatg gggacaagcc aattaccttt gaggtgacca
     2041 taggaaacta cggcaatgaa gtcgatggta tgtcccggcc cctgaggcct cggccccgga
     2101 aagagcctgg ggatgaagaa gaggtagacc tgattcagaa ctccagtgac gatgaaggtg
     2161 acgaagccgg ggacctggcc tcggtgtcct ccaccccacc tatgcggccc cagatcacgg
     2221 acaggaacta tttccacctg ccctacctgg agcgcaagcc ctgcatctat atcaagagct
     2281 ggtggcctga ccagaggcgg cgcctctaca atgccaacat catggatcac attgctgaca
     2341 agctggaaga aggcctgaat gatgtacagg agatgatcaa aacggagaag tcctacccgg
     2401 agcgccgcct gcggggtgtg ctagaggaac tcagctgtgg ctgccaccgc ttcctctccc
     2461 tctcggacaa ggaccagggc cgctcgtccc gcaccaggct ggatcgagag cgtcttaagt
     2521 cctgtatgag ggagttggag agcatgggac agcaggccaa gagcctgagg gctcaggtga
     2581 agcggcacac tgttcgggac aagctgaggt catgccagaa ctttctgcag aagctacgct
     2641 tcctggcgga tgagccccag cacagcattc ctgatgtgtt catttggatg atgagcaaca
     2701 acaaacgtat cgcctatgcc cgcgtgcctt ccaaagacct gctcttctcc atcgtggagg
     2761 aggaactggg caaggactgc gccaaagtca agaccctctt cctgaagctg ccagggaaga
     2821 ggggcttcgg ctcggcaggc tggacagtac aggccaagct ggagctctac ctgtggctgg
     2881 gcctcagcaa gcagcgaaag gacttcctgt gtggtctgcc ctgtggcttc gaggaggtca
     2941 aggcagccca aggcctgggc ctgcattcct ttccgcccat cagcctagtc tacaccaaga
     3001 agcaagcctt ccagctccga gcacacatgt atcaggcccg aagcctcttt gctgctgaca
     3061 gcagtgggct ctctgatccc tttgcccgtg tcttcttcat caaccagagc caatgcactg
     3121 aggttctaaa cgagacactg tgtcccacct gggaccagat gctggtattt gacaacctgg
     3181 agctgtacgg tgaagctcac gagttacgag atgatccccc catcattgtc attgaaatct
     3241 acgaccagga cagcatgggc aaagccgact tcatgggccg gaccttcgcc aagcccctgg
     3301 tgaagatggc agatgaagca tactgcccac ctcgcttccc gccgcagctt gagtactacc
     3361 agatctaccg aggcagtgcc actgccggag acctactggc tgccttcgag ctgctgcaga
     3421 ttgggccatc agggaaggct gacctgccac ccatcaatgg cccagtggac atggacagag
     3481 ggcccatcat gcctgtgccc gtgggaatcc ggccagtgct cagcaagtac cgagtggagg
     3541 tgctgttctg gggcctgagg gacctaaaga gggtgaacct ggcccaggtg gaccgaccac
     3601 gggtggacat cgagtgtgca ggaaaggggg tacaatcctc cctgattcac aattataaga
     3661 agaaccccaa cttcaacacg ctggtcaagt ggtttgaagt ggacctcccg gagaatgagc
     3721 tcctgcaccc acccttgaac atccgagtgg tagattgccg ggcctttgga cgatacaccc
     3781 tggtgggttc ccacgcagtc agctcactga ggcgcttcat ctaccgacct ccagaccgct
     3841 cagcccccaa ctggaacacc acaggggagg ttgtagtaag catggagcct gaggagccag
     3901 ttaagaagct ggagaccatg gtgaaactgg atgcgacttc tgatgctgtg gtcaaggtgg
     3961 atgtggctga agatgagaag gaaaggaaga agaagaaaaa gaaaggcccg tcagaggagc
     4021 cagaggagga agagcccgat gagagcatgc tggattggtg gtccaagtac ttcgcctcca
     4081 tcgacacaat gaaggagcaa cttcgacaac atgagacctc tggaactgac ttggaagaga
     4141 aggaagagat ggaaagcgct gagggcctga agggaccaat gaagagcaag gagaagtcca
     4201 gagctgcaaa ggaggagaaa aagaagaaaa accagagccc tggccctggc cagggatcgg
     4261 aggctcctga gaagaagaaa gccaagatcg atgagcttaa ggtgtacccc aaggagctgg
     4321 aatcggagtt tgacagcttt gaggactggc tgcacacctt caacctgttg aggggcaaga
     4381 cgggagatga tgaggatggc tccacagagg aggagcgcat agtaggccga ttcaagggct
     4441 ccctctgtgt gtacaaagtg ccactcccag aagatgtatc tcgagaagct ggctatgatc
     4501 ccacctatgg aatgttccag ggcatcccaa gcaatgaccc catcaatgtg ctggtccgaa
     4561 tctatgtggt ccgggccaca gacctgcacc cggccgacat caatggcaaa gctgacccct
     4621 atattgccat caagttaggc aagaccgaca tccgagacaa ggagaactac atctccaagc
     4681 agctcaaccc tgtgtttggg aagtcctttg acattgaggc ctccttcccc atggagtcca
     4741 tgttgacagt ggccgtgtac gactgggatc tggtgggcac tgatgacctc atcggagaaa
     4801 ccaagattga cctggaaaac cgcttctaca gcaagcatcg cgccacctgc ggcatcgcac
     4861 agacctattc catacatggc tacaatatct ggagggaccc catgaagccc agccagatcc
     4921 tgacacgcct ctgtaaagag ggcaaagtgg acggccccca ctttggtccc catgggagag
     4981 tgagggttgc caaccgtgtc ttcacggggc cttcagaaat agaggatgag aatggtcaga
     5041 ggaagcccac agatgagcac gtggcactgt ctgctctgag acactgggag gacatccccc
     5101 gggtgggctg ccgccttgtg ccggaacacg tggagaccag gccgctgctc aaccctgaca
     5161 agccaggcat tgagcagggc cgcctggagc tgtgggtgga catgttcccc atggacatgc
     5221 cagcccctgg gacacctctg gatatatccc ccaggaaacc caagaagtac gagctgcggg
     5281 tcatcgtgtg gaacacagac gaggtggtcc tggaagacga tgatttcttc acgggagaga
     5341 agtccagtga catttttgtg agggggtggc tgaagggcca gcaggaggac aaacaggaca
     5401 cagatgtcca ctatcactcc ctcacggggg agggcaactt caactggaga tacctcttcc
     5461 ccttcgacta cctagcggcc gaagagaaga tcgttatgtc caaaaaggag tctatgttct
     5521 cctgggatga gacggagtac aagatccctg cgcggctcac cctgcagatc tgggacgctg
     5581 accacttctc ggctgacgac ttcctggggg ctatcgagct ggacctgaac cggttcccga
     5641 ggggcgctaa gacagccaag cagtgcacca tggagatggc caccggggag gtggacgtac
     5701 ccctggtttc catctttaaa cagaaacgtg tcaaaggctg gtggcccctc ctggcccgca
     5761 atgagaatga tgagtttgag ctcacaggca aagtggaggc ggagctacac ctactcacgg
     5821 cagaggaggc agagaagaac cctgtgggcc tggctcgcaa tgaacctgat cccctagaaa
     5881 aacccaatcg gccggacaca agcttcatct ggttcttgaa ccctctcaag tctgcccgct
     5941 acttcctgtg gcatacctac cgctggctac tcctcaaatt cctgctgctc ttcctcctgc
     6001 tgctgctctt cgccctgttt ctctactctc tgcctggcta cctggccaag aagatccttg
     6061 gggcctgagc cctgcagtcg cctaggcctg ccggcctgac acggcattcg tctggttcct
     6121 gaacccactc aaatctatca agtacctcat ctgcacccgg tacaagtggc tgatcatcaa
     6181 gatcgtgctg gcgctgctgg ggctgctcat gctggccctc ttcctttaca gcctcccagg
     6241 ctacatggtc aagaagctcc taggggcctg aagtgtgccc caccccagcc cgctccagca
     6301 tccctccagg ggctgctgcg tattttgcct tccctcacct ggactctctc ccaactccct
     6361 gaggagccct cccacgcctg ccagccttga gcaagacacc tgcttgctgg acttcatccc
     6421 caccccacac ccaaactgtt gcttgcctga tcttgtccca ggcctgcctg gggtttgggg
     6481 cacagttggc ctccaaaacc agataccctc ttgtctaaag taccaggttc ctctgcccaa
     6541 ccccaagagt ggtagtggcc caaccctccc tgtgctttcc aaatcttgtc ttaaggcacc
     6601 agtgaaatta accaagaaac gcggagcaat gcccaaggct ctgatgagta ggaacacgtg
     6661 gaaagcacca ggaatgccag cagaggcgag gcggcacacc tctctgcaga gcatccaggc
     6721 cgagcggcgg gcagcggcca gctgcttctg cgcatgctct cctcttggct ctgcttcttt
     6781 ctcacagtca cagtcacttc acagcttagc cttgggcttc ccatcacttc caggggtgcc
     6841 tctgccttgg ccagtgtgtg tcagctagta cacaagctcc aagtgtgaat caggtgtact
     6901 ggccgtcctg aagactgact gccctgtcct tcctgccgac agccacaccc gagtgtacac
     6961 ttaaagcggt gcccttctgc ctctgtgggc ctgctggctg ctgttccttt cttgagtgtg
     7021 attttttttt tctctccctc aataaaataa atcaaactct gagac
//
