LOCUS       NM_175489               7152 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus oxysterol binding protein-like 8 (Osbpl8), transcript
            variant 1, mRNA.
ACCESSION   NM_175489
VERSION     NM_175489.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Phua,Y.L., Chen,K.H., Hemker,S.L., Marrone,A.K., Bodnar,A.J.,
            Liu,X., Clugston,A., Kostka,D., Butterworth,M.B. and Ho,J.
  TITLE     Loss of miR-17~92 results in dysregulation of Cftr in nephron
            progenitors
  JOURNAL   Am J Physiol Renal Physiol 316 (5), F993-F1005 (2019)
   PUBMED   30838872
REFERENCE   3  (bases 1 to 7152)
  AUTHORS   Tremblay,M.G. and Moss,T.
  TITLE     Loss of all 3 Extended Synaptotagmins does not affect normal mouse
            development, viability or fertility
  JOURNAL   Cell Cycle 15 (17), 2360-2366 (2016)
   PUBMED   27399837
REFERENCE   4  (bases 1 to 7152)
  AUTHORS   van Kampen,E., Beaslas,O., Hildebrand,R.B., Lammers,B., Van
            Berkel,T.J., Olkkonen,V.M. and Van Eck,M.
  TITLE     Orp8 deficiency in bone marrow-derived cells reduces
            atherosclerotic lesion progression in LDL receptor knockout mice
  JOURNAL   PLoS One 9 (10), e109024 (2014)
   PUBMED   25347070
  REMARK    GeneRIF: Orp8 deficiency in bone marrow-derived cells reduces
            atherosclerotic lesion progression in LDL receptor knockout mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7152)
  AUTHORS   Blumensatt,M., Greulich,S., Herzfeld de Wiza,D., Mueller,H.,
            Maxhera,B., Rabelink,M.J., Hoeben,R.C., Akhyari,P., Al-Hasani,H.,
            Ruige,J.B. and Ouwens,D.M.
  TITLE     Activin A impairs insulin action in cardiomyocytes via
            up-regulation of miR-143
  JOURNAL   Cardiovasc Res 100 (2), 201-210 (2013)
   PUBMED   23812417
REFERENCE   6  (bases 1 to 7152)
  AUTHORS   Jordan,S.D., Kruger,M., Willmes,D.M., Redemann,N., Wunderlich,F.T.,
            Bronneke,H.S., Merkwirth,C., Kashkar,H., Olkkonen,V.M., Bottger,T.,
            Braun,T., Seibler,J. and Bruning,J.C.
  TITLE     Obesity-induced overexpression of miRNA-143 inhibits
            insulin-stimulated AKT activation and impairs glucose metabolism
  JOURNAL   Nat Cell Biol 13 (4), 434-446 (2011)
   PUBMED   21441927
REFERENCE   7  (bases 1 to 7152)
  AUTHORS   Barski,A. and Frenkel,B.
  TITLE     ChIP Display: novel method for identification of genomic targets of
            transcription factors
  JOURNAL   Nucleic Acids Res 32 (12), e104 (2004)
   PUBMED   15252151
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 7152)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   9  (bases 1 to 7152)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 7152)
  AUTHORS   Anniss,A.M., Apostolopoulos,J., Dworkin,S., Purton,L.E. and
            Sparrow,R.L.
  TITLE     An oxysterol-binding protein family identified in the mouse
  JOURNAL   DNA Cell Biol 21 (8), 571-580 (2002)
   PUBMED   12215260
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK037158.1, AK050244.1,
            CF745582.1, BU152326.1, AA536976.1, AK037869.1 and AK052311.1.
            
            On Aug 16, 2004 this sequence version replaced NM_175489.2.
            
            Summary: This gene encodes a member of the oxysterol-binding
            protein (Osbp) family, a group of intracellular lipid receptors.
            Like most members, the encoded protein contains an N-terminal
            pleckstrin homology domain and a highly conserved C-terminal
            Osbp-like sterol-binding domain. Two transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created using sequences from
            two different strains, C57BL/6J and C57BL/6J x DBA/2J F1, because
            no single transcript from the same strain was available for the
            full length of the gene.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.195775.1,
                                           SRR1660817.49455.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-566               AK037158.1         1-566
            567-2180            AK050244.1         531-2144
            2181-2299           AK050244.1         2146-2264
            2300-2647           CF745582.1         342-689
            2648-2665           AK050244.1         2613-2630
            2666-2699           CF745582.1         708-741
            2700-3659           AK050244.1         2663-3622
            3660-4295           BU152326.1         44-679
            4296-4472           AA536976.1         208-384
            4473-4759           AK037869.1         169-455
            4760-4949           AK052311.1         196-385
            4950-6290           AK037869.1         646-1986
            6291-7152           AK052311.1         1726-2587
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 58.24 cM"
     gene            1..7152
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /note="oxysterol binding protein-like 8"
                     /db_xref="GeneID:237542"
                     /db_xref="MGI:MGI:2443807"
     exon            1..395
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            396..501
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     CDS             460..3129
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /note="isoform a is encoded by transcript variant 1;
                     OSBP-related protein 8; oxysterol-binding protein-related
                     protein 8"
                     /codon_start=1
                     /product="oxysterol-binding protein-related protein 8
                     isoform a"
                     /protein_id="NP_780698.2"
                     /db_xref="CCDS:CCDS36056.1"
                     /db_xref="GeneID:237542"
                     /db_xref="MGI:MGI:2443807"
                     /translation="MEAALADGEPDRSSLLGDSKDVLGPSTVVANSDEPQHLTPGKMS
                     QRQGRDANPTPTRDLPQPSLSPASLHSQGFERGKEDISQNKDDSSLSMSKSKSESKLY
                     NGSEKDSSTSSKLTKKESLKVQKKNYREEKKRATKELLSTITDPSVIVMADWLKIRGT
                     LKSWTKLWCVLKPGVLLIYKTQKNGQWVGTVLLNACEIIERPSKKDGFCFKLFHPLEQ
                     SIWAVKGPKGEAVGSITQPLPSSYLIIRATSESDGRCWMDALELALKCSSLLKRTMVR
                     EGKEHDLSISSDSTHVTLYGLLRANNLHSGDNFQLNDSEIERQHFKDQDLYSDKSDKE
                     NDPEHDESDNEVLGKSEESDTDTSERQDDSYIDPEPVEPLKETTYMEQSHEELGEAGE
                     ASQTETVSEENKSLIWTLLKQVRPGMDLSRVVLPTFILEPRSFLDKLSDYYYHADFLS
                     EAALEENPYFRLKKVVKWYLSGFYKKPKGLKKPYNPILGETFRCLWIHPRTNSKTFYI
                     AEQVSHHPPISAFYVSNRKDGFCLSGSILAKSKFYGNSLSAILEGEARLTFLNRGEDY
                     VMTMPYAHCKGILYGTMTLELGGTVNITCQKTGYSAILEFKLKPFLGSSDYVNQISGK
                     LKLGKEVLATLEGHWDSEVFINDKKTDNSEIFWNPTPDIKQWRLIRHTVKFEEQDDFE
                     SEKLWQRVTKAINAKDQTEATQEKYVLEEAQRQAARDRKTKTQEWVCKLFELDPLTGE
                     WHYKFSDTRPWDPLNDMIQFEKDGVIQTKVKHRTPMVSVPKMKHKPTRQQKKVVKGYS
                     SPEPDIQDSSGSEAQSVKPSTRRKKGIDLGDIQSSIESIKQTQEEIKRNIMALRNHLL
                     SSTPATDYFLQQKDYFVIFLLILLQVIINFIFK"
     exon            502..538
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            539..676
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            677..747
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            748..825
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            826..927
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1131
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1216
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1388
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1626
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1811
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1893
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..1992
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2089
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2188
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2286
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2376
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2513
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2514..2699
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2700..2787
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2788..2893
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..2996
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..7152
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7132..7137
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Osbpl8"
                     /gene_synonym="C730029P18Rik; D330025H14Rik; ORP-8"
ORIGIN      
        1 agaaaggcgg tgtcagggcg gatgacagca ttctgctgcg acgcgctccc agctgggcgg
       61 cggtggtagc ggcagcggca gcggcagcgg cagccggggc ggcagcggcg ttcgactcgg
      121 ccaccgccga gagcggtagc ggtagagaag gaagaggttc cgccctggcc cgcgggcgga
      181 ggagcccgag ttcgctcgcc gctccgggcc gagcccccgc cgcggctcgc ctgtgctctc
      241 cgtccgggct ggctctcagt tcccgcgggc gaccgcagcc gagagaagtt gaggaggggg
      301 ggtcccaccg gacactaagg ccccggactc cggcgtccct gtgatcccac ggaagcctgc
      361 tccctgcggg ccgcctcctc tggggctccg catcgggttt ccagacatat aggatggcta
      421 accattaatg gaagggaaag cataagtcta tcgtccatta tggaggcagc cttagcagac
      481 ggagaacctg acagatcttc gcttcttggt gatagcaaag atgtccttgg gccatcaact
      541 gttgtagcga acagtgacga acctcagcat ttgaccccag gaaagatgag tcagcgccaa
      601 ggcagagacg ctaacccaac cccaactaga gatctgcctc agccttctct cagccccgcc
      661 agccttcaca gccagggttt tgaaagagga aaagaagaca tttctcaaaa taaagatgac
      721 tcttcacttt caatgtcaaa gagcaagtct gaatctaaac tatataatgg ctcagaaaag
      781 gacagttcaa cttcaagtaa actcacgaag aaagagtctc ttaaggtaca aaagaaaaac
      841 taccgtgaag agaagaagag agccacgaag gagttactca gcaccatcac agacccctct
      901 gtcattgtca tggccgactg gctgaagatt cgtggtaccc tgaagagctg gaccaagctg
      961 tggtgtgtgt tgaaacctgg cgtgcttctg atctataaaa cacaaaaaaa tggtcagtgg
     1021 gtcggaacag tccttctgaa tgcttgtgag atcattgaac gtccatcaaa aaaggatggc
     1081 ttttgtttca agcttttcca tcctttggag cagtctattt gggcagtgaa gggtcctaaa
     1141 ggtgaagcgg ttggctccat aacccagcct ctacctagca gctacttgat tatccgagct
     1201 acttcagagt cagatggaag gtgctggatg gatgctttgg agttggcttt gaagtgttcc
     1261 agtctcctta agcggaccat ggtgagggag gggaaggagc acgacctgag catctcctca
     1321 gacagcacac acgtgacgct gtatggctta ctgcgcgcta acaatctcca cagtggcgac
     1381 aacttccagt tgaatgacag tgagatcgaa agacagcact ttaaggacca agatttgtac
     1441 tctgataaat ctgacaagga gaacgacccg gagcacgatg agtctgacaa cgaggtgctg
     1501 gggaagagcg aggagagcga cacagacacg tccgaaaggc aagacgactc gtacatcgac
     1561 cctgagcctg tggagccctt aaaggagacc acctacatgg agcagagcca cgaagaactg
     1621 ggagaggcag gtgaggcctc tcaaacggaa actgtgtctg aagaaaacaa aagccttatc
     1681 tggacgctgc tcaagcaagt ccgtcccggc atggacctat ccagggtggt tcttcctacg
     1741 tttattttgg agccccgctc tttcctggat aagctttcag attactacta ccacgccgat
     1801 ttcctgtctg aagctgctct agaagaaaat ccgtatttcc gtttgaagaa agtcgtgaaa
     1861 tggtatttgt caggatttta taagaagcca aagggactaa agaaaccata taatcctata
     1921 cttggtgaga ctttccgatg tttatggatt catcccagaa caaacagcaa aactttctat
     1981 attgctgaac aggtgtccca tcacccacca atatctgcct tttatgtcag taaccggaaa
     2041 gatgggtttt gccttagtgg tagcatcctg gctaagtcca agttttatgg aaactcgtta
     2101 tctgccatat tggagggaga agcccggtta actttcttga acagaggtga agattacgtc
     2161 atgacaatgc catatgctca ctgtaaagga attctttatg gcacaatgac cctggagctt
     2221 ggtggaactg tcaatattac ctgtcagaag actggataca gtgcgatact tgaatttaaa
     2281 ctaaagccat ttctagggag tagtgattat gttaaccaaa tatcagggaa acttaaattg
     2341 ggaaaagaag tcctagctac tttggaaggc cattgggata gtgaagtttt cattaatgac
     2401 aaaaagactg ataactcaga gattttctgg aatccaactc ctgacattaa acagtggaga
     2461 ttaataaggc acactgtaaa gtttgaagaa caggatgatt tcgaatcaga gaaactctgg
     2521 caacgagtga caaaagctat aaatgccaaa gaccaaacgg aagctactca agaaaagtat
     2581 gttctggaag aagctcaaag acaagctgcc agagatcgaa aaacaaaaac tcaagagtgg
     2641 gtttgtaagt tatttgaact tgacccactc actggagaat ggcactataa gttttcagat
     2701 actcgaccat gggacccact taatgatatg atacaatttg aaaaagatgg agttatccag
     2761 accaaagtga aacatcgcac tccaatggtt agtgttccaa aaatgaaaca taaaccaaca
     2821 aggcagcaga agaaagtggt aaaaggctat tcttccccag aacctgacat ccaggattcc
     2881 tctggaagtg aagctcaatc tgtaaaacca agtacacgaa ggaagaaggg gatagacctg
     2941 ggagacattc agagttccat tgagtctata aagcagacac aggaagagat taaaagaaat
     3001 attatggctc ttcgaaatca tttactttca agtacacctg ctacagatta ttttctgcaa
     3061 cagaaagact acttcgtcat tttccttctg attttgcttc aagtcataat aaactttata
     3121 tttaagtaga agttctcaac attgaatcca tgaacaagaa agattagatg tgatctggga
     3181 ccattgttca aattggcttg ctctttatta aagcatcaca acaaacaaaa ctaggagata
     3241 aatgtagaaa tgtctaccta cttttcacat cttttatcct taacccaaaa caagagctac
     3301 tgtgtttggt tactacaatg ggctgtgtgt taagtgccag aacacttgta tgagactgtg
     3361 cctacacgtg agtatcgtag acccacgtgt gtataaagag ctcttccctg acagcagttc
     3421 ctgtgttcta cagtgtgctg tgacgtattc tgataccagt gttcataaca aagatgccgt
     3481 cactcctata aatacatcta gggtcaacta ttgtcttcag aagaccggca tataactttc
     3541 attagggaat tacagtggga aaatggtaga taatagacat attatgagta atgtcaatac
     3601 attatactgt taaaaccatc tccaagttac ctagttcagt tagtcttctt agatacacca
     3661 agaaaaaata cgtgagtgat cagaattaat atattgtaag ggacaagtag aaaattttta
     3721 agagtttaca caagattttc taacccctag catttaatgt tacagatata ggtgaataca
     3781 tagttatgtg tgtgtgtata tatatatatc tcactattaa agactggtaa gcagctacat
     3841 ggatctatat gttacaatcc caacttaagc acttgctgaa aaggaacggt cagaggtgtt
     3901 taagtaggtt atgtgaaatc ctgatgattc ggtgttttct ttatgaaaaa ctttttagct
     3961 ttcatttaga cacaagagta ctcaaggagt aaatagaaga gaaaaatgta atatcttact
     4021 tcaaaagtca aaaaaggtgg taagaacaga ttgcagtggc ctttaaaaca ttaaaataaa
     4081 attgcttaaa tgtgcaataa gttgagcatt ggagaatgtc aagttatgaa gtttgtcatc
     4141 tcctcccatc atcctgtggc aagccagttc tgtacaagtc tttctgctct gctcaggcag
     4201 atttgttcct aagaggtttt gcctatttat tcagtttaat aattgtgttt ttacataaat
     4261 atttattatt atcaaaaaca cttctttaaa aggtagttaa gttttgaaac atcataaaat
     4321 gtagattgtt caaactacag gttttagaag ggtttgttga gtgacaacta agaaagagct
     4381 gctgttggct gcatcggcct tcagctgaga agagattgag ctacaaggaa agctaactct
     4441 gctgtgagag gacagctgca gtgctcacac tgaaagcctc ttctgatgaa tacaggtgtc
     4501 cttatgttcc tatgaacagc cttttacagt gtggtgttag caaaacaatg ctgtatttca
     4561 tatgaaaata ttttaaactt ttaacagcat aagcaagatg tacctgtatc cagtatttca
     4621 ttatggagtt ttgggttata tttcatattt gtcaatatat tgtttgtatt catattctac
     4681 taccaatatt atgctcaact gcctattcat tgatgtgata ttttgtaaat attgtacaat
     4741 ttgatcttta tatgatttta aattactatt aatttatatt atatataact tgattggttg
     4801 atcacgaaaa gtcttttcat tgttaaaccc tgtaatgtaa aattgttggt gacatgcctt
     4861 agagctcttt ctaaatcctt tgtgttgttt gtgatctgaa cagtgcttta tttgtgttga
     4921 tatccacgac tggaaaggta attgttgggt tttttttttt aatttcactt tagagcttat
     4981 gttaatggat ccttacccaa ggagtcagaa actaatgaac agggtgactg tttcttatac
     5041 actaaaagct tatgttcaat caaatgcttt tctgatgctt ttcgttgtat gagtttcttt
     5101 ctgtgaactg aacacaaaac tcaggaattg gaggattcat atgtaagtcc tttaaacaac
     5161 atataattga acttgagttt agccagtgtg tgacagacct cgaacaatct gatctaaggg
     5221 ttaactttct tcataaatgg attcataatg tatatgtgac aaactgttcc aaacttgctg
     5281 aaaactcatt gtgatttcta gggtttctga ctatgaccag ttttgtttgt tttgtttttc
     5341 atctatccaa aacttcattt tctcacataa aaatttagat aaccacaatt attgacacag
     5401 ttgttaaatg tctttagttt tctgccattt agtgagatat tgctggctaa gacaagacaa
     5461 taacaggcat gtgtggtgtt agtgtgctgc agcaatcgat ttaacttgta attcctacca
     5521 cttctgtctc tagtatgact tggaaagcgg cagtggtcat ctttgtcaca gctcagttga
     5581 cagctgcatt cccttaaaaa gttggccttg tgagatacaa tcctcattta atatttcatg
     5641 cttttgtgcc tttaaggaaa tatttttgtt agttttttgt tacagaacca taatgtgttc
     5701 aaagtgtttg tagcttattt gtcataaaag ggtcatttgt ctgggtcact gtgtttcctt
     5761 ttatctagct gaagaatatg taaacagtaa tgccgtactt ttataagggt ttgtgtagta
     5821 actatttcaa atatattcat actcagacat caatgaagta aacttgtcag atggctctga
     5881 gtattgtaga cagtgccttt ttgatggaat tcacactgtt ttcgggtgtc aacttgtgcc
     5941 atattcacag aattttgtgg aaggcagttt taactttttg aagaatatcg tcatcaaaat
     6001 tttaaaaaac aaatgtataa aacttcatct tttttcagtg tttagcattt ctagtcagca
     6061 gtaaatagtc tggttttgct tttgtttttg tcttatttca ggtatgatgg catacgtacg
     6121 cgatgagcca tacacgagac tgtagactat tacattaaat ataaataaag aacatgttcg
     6181 tttatacaaa ttttccgatt tgacccagtg ggagagacct ttcataattc aataagttta
     6241 catttcaatg tctttcatat ttgactacat ataaagagat tctttaaaat gtataacctg
     6301 ccaccactgc ataatttggt ttcattttgt tatgtattgt gtgggtttag tatatccaat
     6361 ttttgttaca attaaaggac tattttgtta cagtttttaa ttgaagatgg actgttaaca
     6421 ttgtacagga ccagtgtctt attaataaga ttaacatatt tagtgagagc cacaggaaaa
     6481 ccttgacaaa aatgaatgtg actatacttt atgaagtatt tcgaaaaatc agcctcttta
     6541 tttatcataa aattatttta atatcataat attgatgttc tttttttttt gttttgtttt
     6601 gttttttgtt tttttcgaga cagggtttct ctgtgtagcc ctggctgtcc tggaactcat
     6661 tctgtagacc aggctggcct tgaactcaga aatccgcctg cctctgcctc ccgagtgctg
     6721 ggattaaagg tgtgtgccac cacacccggc taatattgat aatctttatt tgaaaaaatg
     6781 tcatgaacca tttgaatgat gagccacaga acctcagttg aatttatttc cacttttggc
     6841 atgttaaata tagatttaat tttaagtact tcagttaatg ggtttataaa gtcaagcact
     6901 agcattggtc agttttgtat gataggatgt aagtgtgttc tcacctgcag tgtaaataca
     6961 gcacactgta gaattctctt aaggtgcata gtaaatgtat agatagtcac aggcggtttt
     7021 gtaatgtata catttctaat ctattattcc taacctgtca tgtttgcaga gagaaaagaa
     7081 tttttctaat gatctgtaaa attatgttaa cttctacaag taggtattct aaataaactt
     7141 ttttaaaaga gc
//
