LOCUS       NM_152818               4097 bp    mRNA    linear   ROD 12-DEC-2024
DEFINITION  Mus musculus oxysterol binding protein 2 (Osbp2), transcript
            variant 1, mRNA.
ACCESSION   NM_152818 XM_483975
VERSION     NM_152818.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ge,T., Yuan,L., Xu,L., Yang,F., Xu,W., Niu,C., Li,G., Zhou,H. and
            Zheng,Y.
  TITLE     Coiled-coil domain containing 159 is required for spermatid head
            and tail assembly in micedagger
  JOURNAL   Biol Reprod 110 (5), 877-894 (2024)
   PUBMED   38236177
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Udagawa,O., Ito,C., Ogonuki,N., Sato,H., Lee,S., Tripvanuntakul,P.,
            Ichi,I., Uchida,Y., Nishimura,T., Murakami,M., Ogura,A., Inoue,T.,
            Toshimori,K. and Arai,H.
  TITLE     Oligo-astheno-teratozoospermia in mice lacking ORP4, a
            sterol-binding protein in the OSBP-related protein family
  JOURNAL   Genes Cells 19 (1), 13-27 (2014)
   PUBMED   24245814
  REMARK    GeneRIF: ORP4 is essential for the postmeiotic differentiation of
            germ cells
REFERENCE   3  (bases 1 to 4097)
  AUTHORS   Anniss,A.M., Apostolopoulos,J., Dworkin,S., Purton,L.E. and
            Sparrow,R.L.
  TITLE     An oxysterol-binding protein family identified in the mouse
  JOURNAL   DNA Cell Biol 21 (8), 571-580 (2002)
   PUBMED   12215260
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK049816.1 and BC058356.1.
            
            On May 10, 2018 this sequence version replaced NM_152818.2.
            
            Summary: The protein encoded by this gene belongs to the
            oxysterol-binding protein-related family of proteins, which are
            defined by a C-terminal sterol domain with a highly conserved
            EQVSHHPP motif. Oxysterols are oxygenated derivatives of
            cholesterol that are involved in mechanisms that include apoptosis,
            cholesterol homeostasis, lipid trafficking and cell
            differentiation. This protein is selectively expressed at high
            levels in the brain and testis. Within the testis, the mRNA is
            localized to postmeiotic germ cells, including spermatids and
            spermatozoa, but is not detectable in somatic cells. Mice
            homozygous mutant for a targeted deletion in this gene do not
            exhibit overt developmental phenotypes but are male sterile.
            Females display normal fertility. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Oct 2014].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            partial transcripts because no single transcript was available for
            the full length of the gene. The full-length structure is inferred
            from partial transcripts, RNA-seq data and orthologous alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.131757.1,
                                           SRR1660817.3301.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1137              AK049816.1         2-1138
            1138-4097           BC058356.1         281-3240
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 2.6 cM"
     gene            1..4097
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="oxysterol binding protein 2"
                     /db_xref="GeneID:74309"
                     /db_xref="MGI:MGI:1921559"
     exon            1..671
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..2763
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="oxysterol-binding protein 2 isoform 1"
                     /protein_id="NP_690031.2"
                     /db_xref="CCDS:CCDS24367.1"
                     /db_xref="GeneID:74309"
                     /db_xref="MGI:MGI:1921559"
                     /translation="MGKAAALSRGGGCAGRSRGLSSLFTVVPCLSCHTAAPGMNSSAF
                     GSGPASKPQLQPVQAPERELLSKQVCQPISEPASRSEPGSQTTSVPRPSGVGQESELQ
                     GLWPGSENGTRSVSIIKASPELAMPSPLQSTVGSLPVTKPESKLVPKTQSFLRQGQAK
                     ISVGTPVSGIGVQMVSPPLDSYKGWLLKWTNYLKGYQRRWFVLGNGLLSYYRNQGEMA
                     HTCRATINLASTHFETEDSCGILLCNGARTYHLKASSEVDRQHWITALELAKAKAIRV
                     MKTQSDDSGDDDEEPAAPADNSELHHTLKTLSLKLNDLSTCNDLIAKHGAALQRSLNE
                     LDSLKIPSECGEKLKVVNERATLFRITSNAMINACRDFLELAETHSRKWQRALNYEQE
                     QRVHLEETIEQLAKQHNSLERAFCNTPGGPASSSKSFSEGSFLTSKGENSEEDEDTEY
                     FDAMEDSTSFITVVTEAKEDRKPESGPGTTTVDWTSADNVLDGASFMPKNSCKVKRRV
                     RIPDKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYHHLLDKAVN
                     CTSSVEQMCLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRMEDMGLRSLCEQVSHH
                     PPSAAHHVFSKHGWSLWQEITIASKFRGKYISIMPLGAIHLEFQASGNHYVWRKSTST
                     VHNIIVGKLWIDQSGDIEIVNHKTKDRCQLKFVPYSYFSKEAARKVTGVVSDSQGKAH
                     YVLSGSWDDQMECSKIVHSSPSSDGRQKTVYQTLPAKLLWRKYPLPENAENMYYFSEL
                     ALTLNEQEDGVAPTDSRLRPDQRLMERGRWDEANTEKQRLEEKQRLSRRRRLESCTAG
                     CGGEEEKESDGYVPLWFEKRLDPLTGEMACMYKGGYWEAKEKKDWHMCPNIF"
     exon            672..880
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1137
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1330
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1448
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1503
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1650
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1896
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2017
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2121
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2217
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2393
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2620
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..4097
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2779..2784
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="hexamer: AATACA"
     polyA_site      2799
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="major polyA site"
     regulatory      4068..4073
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="hexamer: AATAAA"
     polyA_site      4097
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="major polyA site"
ORIGIN      
        1 cactccgcgc tccgccgagc tcgggtccac agctctatgg ggaaagcggc cgctctgagc
       61 cgaggcggcg gctgcgctgg ccgctcccgc gggctctcgt cgctgttcac ggttgtgccc
      121 tgcttgtcct gccacacggc ggcgccgggc atgaactcat ccgcgttcgg ctcaggacca
      181 gcgtcaaagc ctcagctcca gcctgtgcag gcaccagagc gggagttgct gtcgaaacag
      241 gtgtgccagc caatttctga gccggcgtcg agatccgaac ctgggtccca gacgacgtct
      301 gtgccaaggc cttcaggggt tgggcaggaa tcggaactac agggcttgtg gccggggtca
      361 gagaacggca caaggtctgt gtccatcatc aaagcttcgc cagagctggc aatgccgtcc
      421 ccgctgcagt caacagtagg gagtttgccc gtcaccaaac ccgagtccaa gctggtgcca
      481 aagacccagt cttttctgcg acaaggacag gcgaagattt ctgtcgggac tccggtgtca
      541 gggattggtg tccaaatggt ctcaccacct ctggacagct acaagggttg gctcctcaag
      601 tggactaact acctgaaggg ctaccagcgc cgttggttcg tgctcggcaa tggcctgctg
      661 tcctactaca gaaaccaggg tgaaatggcc cacacctgcc gtgcgaccat caacttggcc
      721 tccacacact ttgaaacaga ggactcttgt ggaatccttc tatgcaacgg ggcaaggacc
      781 taccacctca aggccagctc agaggtggac cggcagcatt ggatcaccgc tctggagctg
      841 gccaaggcca aggctatccg tgtgatgaaa actcagtcag atgattctgg ggacgacgac
      901 gaagagcctg ctgccccagc tgacaacagt gaactccacc acactcttaa gaccctctca
      961 ctgaagttaa atgacctcag cacgtgcaat gacctcattg ccaagcatgg cgctgccctc
     1021 cagcgctccc tgaatgagct ggacagcctc aagatcccat cagagtgcgg ggagaaactg
     1081 aaggttgtga acgagcgggc caccctcttc cgcatcacat caaatgctat gatcaacgcc
     1141 tgtagggact tcctggaact agctgagact cacagccgga aatggcagcg ggctctaaat
     1201 tatgagcagg agcagcgtgt ccacctggaa gagacaattg agcagttggc caagcagcac
     1261 aacagtcttg aacgggcctt ctgcaacacc cctggcgggc cagccagctc cagcaagagc
     1321 ttcagcgagg gaagcttctt gacttccaaa ggagagaaca gtgaggaaga tgaagatact
     1381 gagtactttg acgccatgga agactctaca tctttcatca ccgtagtcac tgaggccaag
     1441 gaagacagaa agcctgagag tggacctggg accaccactg tggactggac ctcagcagat
     1501 aatgtattag atggtgcctc attcatgccc aagaattcat gcaaagttaa gaggcgagtc
     1561 cgcatccctg acaaacccaa ctatagcctt aacctctgga gcatcatgaa gaattgtatt
     1621 ggccgagagc tttcccggat ccccatgccg gtgaacttca atgagcccct gtccatgctc
     1681 cagcgactta cagaggacct ggagtaccac cacctgctgg acaaggcggt gaactgcacc
     1741 agctcggtgg agcagatgtg cctggtagcc gccttttctg tgtcctccta ctccaccacg
     1801 gtgcaccgca tcgccaagcc cttcaaccct atgctcgggg agaccttcga gctggaccgt
     1861 atggaggaca tgggcctgcg ttccctctgt gagcaggtga gccaccaccc cccgtctgct
     1921 gcccaccacg tgttctctaa gcatggctgg agcctctggc aagaaatcac catcgccagc
     1981 aagttccgag ggaaatacat ctctatcatg ccactcggtg ccatccacct agaattccag
     2041 gccagtggca atcactacgt gtggaggaag agcacctcca ccgtgcacaa catcatcgtg
     2101 ggcaagctct ggattgacca gtcaggggac attgagattg tgaaccacaa gaccaaggac
     2161 cggtgccagc tgaagttcgt accctacagc tacttctcca aagaggcagc ccgaaaggtg
     2221 actggagtgg tgagtgacag ccagggcaag gcccactacg tgctgtcagg ttcgtgggat
     2281 gaccagatgg aatgttctaa gattgtgcac agcagcccca gctctgatgg gagacagaaa
     2341 accgtgtacc agacactgcc cgccaaactg ctctggagga aatacccact gccggagaat
     2401 gcggagaaca tgtactattt ctccgagcta gccctgaccc tcaacgagca ggaggacggc
     2461 gtggcgccca ccgacagtcg cctgcggcca gaccagcggc ttatggagag gggacgctgg
     2521 gacgaggcca acaccgagaa gcaacggctg gaggagaagc agcgcctgtc caggcgacgg
     2581 cggctggagt catgcacggc aggctgcggt ggggaggaag agaaggagtc agatggctat
     2641 gtgccgctct ggttcgagaa gaggctggac ccgctgactg gggagatggc ctgcatgtac
     2701 aagggcggct actgggaggc caaggagaag aaggactggc acatgtgccc caacatcttc
     2761 tgagccactc ctgcggcaaa tacaggcgcc tgcactgcct gccagccttt tctttaatgc
     2821 acccaattta ctacagaacc cacacacgct ttcgaagggg taagggctga cctgggttct
     2881 tgccagcccc cagggtgtcg ggtcacctgt gcccgttatg attgacttgg gccccatggg
     2941 caccctagtt cccagttccc ataactcagg ccggctggtc gggccatggg ctgcccaggt
     3001 cactaacccc accccacccc cgctagggag tctcttttga cttttttaag gaaaaaaaat
     3061 ctccatttct ttccaacgat gaggtttagt aaatattttt tagcgtatca cttaaaagac
     3121 tcactttcca agtgtttgtt cttgccacat aactatcctg gcaagaagcc cttttttaaa
     3181 gactcgagga agccaggccc ctttgaatgg ggagcacttt taacacagcc aggctttcta
     3241 tctggactgt ccaagctcca gggacgcaac accacggagg cagcaggagg cctggctgac
     3301 accgcagcat agtgtgcaga gcccgtgtgg gggggggggg aacagaggaa ggtagaaggc
     3361 aaacatgccc ccccaccccg tgccaggtct tggccatgct gggacagcac cccaggccca
     3421 gagacaccaa gaagggctga aaggcagggc ccttaggtgc ttcctgccca agaatcaggc
     3481 tcccaagtca gcaataagga acaaaccttc atttagcctg ggctggtgac ccgggctcct
     3541 gcacctggcc tcttgctgtc cttaggcggc atcagggtgc ctctcctcag cactcctgac
     3601 atctttgttt gcctaattta tatatataat atacaaatat atataacata actattttgc
     3661 ttaaatttct atgatacgtg aaagtgaaaa atgatgaaga caggagtgcc tgtctgagtt
     3721 tggcctcctg tcggccctgc cctcaccttg tagtcctccc agtggcttct gccaacctta
     3781 gacctgaaac ctgtccctca gaccctcagc ctccaaccca tttcacttca gtttgatctg
     3841 gaaagttcct tgcagggcct ttctttctac tccaggcctg gttttgattg ttttgttttg
     3901 ttttttattt aaaaaaagat tgagaaagag aaaacaccac gtatgaaatg ccttacaaca
     3961 cccactctag agacggggtg gggaaggaga gggagagggc acctggtctc cgtcagagct
     4021 gggctgtacc tcttcccagc aggcgtgagc aaacaccttc tgaccacaat aaaacccttg
     4081 ttctggagtt ttccaca
//
