LOCUS       NM_001363163            4106 bp    mRNA    linear   ROD 12-DEC-2024
DEFINITION  Mus musculus oxysterol binding protein 2 (Osbp2), transcript
            variant 4, mRNA.
ACCESSION   NM_001363163 XM_017314808
VERSION     NM_001363163.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ge,T., Yuan,L., Xu,L., Yang,F., Xu,W., Niu,C., Li,G., Zhou,H. and
            Zheng,Y.
  TITLE     Coiled-coil domain containing 159 is required for spermatid head
            and tail assembly in micedagger
  JOURNAL   Biol Reprod 110 (5), 877-894 (2024)
   PUBMED   38236177
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Udagawa,O., Ito,C., Ogonuki,N., Sato,H., Lee,S., Tripvanuntakul,P.,
            Ichi,I., Uchida,Y., Nishimura,T., Murakami,M., Ogura,A., Inoue,T.,
            Toshimori,K. and Arai,H.
  TITLE     Oligo-astheno-teratozoospermia in mice lacking ORP4, a
            sterol-binding protein in the OSBP-related protein family
  JOURNAL   Genes Cells 19 (1), 13-27 (2014)
   PUBMED   24245814
  REMARK    GeneRIF: ORP4 is essential for the postmeiotic differentiation of
            germ cells
REFERENCE   3  (bases 1 to 4106)
  AUTHORS   Anniss,A.M., Apostolopoulos,J., Dworkin,S., Purton,L.E. and
            Sparrow,R.L.
  TITLE     An oxysterol-binding protein family identified in the mouse
  JOURNAL   DNA Cell Biol 21 (8), 571-580 (2002)
   PUBMED   12215260
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731853.9 and AL691413.5.
            
            On May 10, 2018 this sequence version replaced XM_017314808.1.
            
            Summary: The protein encoded by this gene belongs to the
            oxysterol-binding protein-related family of proteins, which are
            defined by a C-terminal sterol domain with a highly conserved
            EQVSHHPP motif. Oxysterols are oxygenated derivatives of
            cholesterol that are involved in mechanisms that include apoptosis,
            cholesterol homeostasis, lipid trafficking and cell
            differentiation. This protein is selectively expressed at high
            levels in the brain and testis. Within the testis, the mRNA is
            localized to postmeiotic germ cells, including spermatids and
            spermatozoa, but is not detectable in somatic cells. Mice
            homozygous mutant for a targeted deletion in this gene do not
            exhibit overt developmental phenotypes but are male sterile.
            Females display normal fertility. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Oct 2014].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.144627.1,
                                           SRR7345562.4916183.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-671               AL731853.9         154240-154910       c
            672-880             AL731853.9         110844-111052       c
            881-1137            AL731853.9         17326-17582         c
            1138-1330           AL731853.9         8801-8993           c
            1331-1448           AL731853.9         8104-8221           c
            1449-1512           AL731853.9         6495-6558           c
            1513-1659           AL731853.9         6095-6241           c
            1660-1905           AL731853.9         5478-5723           c
            1906-2026           AL731853.9         3466-3586           c
            2027-2130           AL731853.9         3133-3236           c
            2131-2226           AL731853.9         2965-3060           c
            2227-2402           AL731853.9         2701-2876           c
            2403-2629           AL691413.5         158443-158669       c
            2630-4106           AL691413.5         156785-158261       c
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 2.6 cM"
     gene            1..4106
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="oxysterol binding protein 2"
                     /db_xref="GeneID:74309"
                     /db_xref="MGI:MGI:1921559"
     exon            1..671
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..2772
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="isoform 4 is encoded by transcript variant 4"
                     /codon_start=1
                     /product="oxysterol-binding protein 2 isoform 4"
                     /protein_id="NP_001350092.1"
                     /db_xref="GeneID:74309"
                     /db_xref="MGI:MGI:1921559"
                     /translation="MGKAAALSRGGGCAGRSRGLSSLFTVVPCLSCHTAAPGMNSSAF
                     GSGPASKPQLQPVQAPERELLSKQVCQPISEPASRSEPGSQTTSVPRPSGVGQESELQ
                     GLWPGSENGTRSVSIIKASPELAMPSPLQSTVGSLPVTKPESKLVPKTQSFLRQGQAK
                     ISVGTPVSGIGVQMVSPPLDSYKGWLLKWTNYLKGYQRRWFVLGNGLLSYYRNQGEMA
                     HTCRATINLASTHFETEDSCGILLCNGARTYHLKASSEVDRQHWITALELAKAKAIRV
                     MKTQSDDSGDDDEEPAAPADNSELHHTLKTLSLKLNDLSTCNDLIAKHGAALQRSLNE
                     LDSLKIPSECGEKLKVVNERATLFRITSNAMINACRDFLELAETHSRKWQRALNYEQE
                     QRVHLEETIEQLAKQHNSLERAFCNTPGGPASSSKSFSEGSFLTSKGENSEEDEDTEY
                     FDAMEDSTSFITVVTEAKEDRKPESGPGTTTVDWTSADNVCEVLDGASFMPKNSCKVK
                     RRVRIPDKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYHHLLDK
                     AVNCTSSVEQMCLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRMEDMGLRSLCEQV
                     SHHPPSAAHHVFSKHGWSLWQEITIASKFRGKYISIMPLGAIHLEFQASGNHYVWRKS
                     TSTVHNIIVGKLWIDQSGDIEIVNHKTKDRCQLKFVPYSYFSKEAARKVTGVVSDSQG
                     KAHYVLSGSWDDQMECSKIVHSSPSSDGRQKTVYQTLPAKLLWRKYPLPENAENMYYF
                     SELALTLNEQEDGVAPTDSRLRPDQRLMERGRWDEANTEKQRLEEKQRLSRRRRLESC
                     TAGCGGEEEKESDGYVPLWFEKRLDPLTGEMACMYKGGYWEAKEKKDWHMCPNIF"
     exon            672..880
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1137
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1330
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1448
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1512
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..1659
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..1905
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2026
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2130
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2226
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2402
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2629
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..4106
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2788..2793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="hexamer: AATACA"
     polyA_site      2808
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="major polyA site"
     regulatory      4077..4082
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="hexamer: AATAAA"
     polyA_site      4106
                     /gene="Osbp2"
                     /gene_synonym="1700095P05Rik; C630001G20Rik; Gm244; ORP-4;
                     OSBPL1"
                     /note="major polyA site"
ORIGIN      
        1 cactccgcgc tccgccgagc tcgggtccac agctctatgg ggaaagcggc cgctctgagc
       61 cgaggcggcg gctgcgctgg ccgctcccgc gggctctcgt cgctgttcac ggttgtgccc
      121 tgcttgtcct gccacacggc ggcgccgggc atgaactcat ccgcgttcgg ctcaggacca
      181 gcgtcaaagc ctcagctcca gcctgtgcag gcaccagagc gggagttgct gtcgaaacag
      241 gtgtgccagc caatttctga gccggcgtcg agatccgaac ctgggtccca gacgacgtct
      301 gtgccaaggc cttcaggggt tgggcaggaa tcggaactac agggcttgtg gccggggtca
      361 gagaacggca caaggtctgt gtccatcatc aaagcttcgc cagagctggc aatgccgtcc
      421 ccgctgcagt caacagtagg gagtttgccc gtcaccaaac ccgagtccaa gctggtgcca
      481 aagacccagt cttttctgcg acaaggacag gcgaagattt ctgtcgggac tccggtgtca
      541 gggattggtg tccaaatggt ctcaccacct ctggacagct acaagggttg gctcctcaag
      601 tggactaact acctgaaggg ctaccagcgc cgttggttcg tgctcggcaa tggcctgctg
      661 tcctactaca gaaaccaggg tgaaatggcc cacacctgcc gtgcgaccat caacttggcc
      721 tccacacact ttgaaacaga ggactcttgt ggaatccttc tatgcaacgg ggcaaggacc
      781 taccacctca aggccagctc agaggtggac cggcagcatt ggatcaccgc tctggagctg
      841 gccaaggcca aggctatccg tgtgatgaaa actcagtcag atgattctgg ggacgacgac
      901 gaagagcctg ctgccccagc tgacaacagt gaactccacc acactcttaa gaccctctca
      961 ctgaagttaa atgacctcag cacgtgcaat gacctcattg ccaagcatgg cgctgccctc
     1021 cagcgctccc tgaatgagct ggacagcctc aagatcccat cagagtgcgg ggagaaactg
     1081 aaggttgtga acgagcgggc caccctcttc cgcatcacat caaatgctat gatcaacgcc
     1141 tgtagggact tcctggaact agctgagact cacagccgga aatggcagcg ggctctaaat
     1201 tatgagcagg agcagcgtgt ccacctggaa gagacaattg agcagttggc caagcagcac
     1261 aacagtcttg aacgggcctt ctgcaacacc cctggcgggc cagccagctc cagcaagagc
     1321 ttcagcgagg gaagcttctt gacttccaaa ggagagaaca gtgaggaaga tgaagatact
     1381 gagtactttg acgccatgga agactctaca tctttcatca ccgtagtcac tgaggccaag
     1441 gaagacagaa agcctgagag tggacctggg accaccactg tggactggac ctcagcagat
     1501 aatgtatgtg aggtattaga tggtgcctca ttcatgccca agaattcatg caaagttaag
     1561 aggcgagtcc gcatccctga caaacccaac tatagcctta acctctggag catcatgaag
     1621 aattgtattg gccgagagct ttcccggatc cccatgccgg tgaacttcaa tgagcccctg
     1681 tccatgctcc agcgacttac agaggacctg gagtaccacc acctgctgga caaggcggtg
     1741 aactgcacca gctcggtgga gcagatgtgc ctggtagccg ccttttctgt gtcctcctac
     1801 tccaccacgg tgcaccgcat cgccaagccc ttcaacccta tgctcgggga gaccttcgag
     1861 ctggaccgta tggaggacat gggcctgcgt tccctctgtg agcaggtgag ccaccacccc
     1921 ccgtctgctg cccaccacgt gttctctaag catggctgga gcctctggca agaaatcacc
     1981 atcgccagca agttccgagg gaaatacatc tctatcatgc cactcggtgc catccaccta
     2041 gaattccagg ccagtggcaa tcactacgtg tggaggaaga gcacctccac cgtgcacaac
     2101 atcatcgtgg gcaagctctg gattgaccag tcaggggaca ttgagattgt gaaccacaag
     2161 accaaggacc ggtgccagct gaagttcgta ccctacagct acttctccaa agaggcagcc
     2221 cgaaaggtga ctggagtggt gagtgacagc cagggcaagg cccactacgt gctgtcaggt
     2281 tcgtgggatg accagatgga atgttctaag attgtgcaca gcagccccag ctctgatggg
     2341 agacagaaaa ccgtgtacca gacactgccc gccaaactgc tctggaggaa atacccactg
     2401 ccggagaatg cggagaacat gtactatttc tccgagctag ccctgaccct caacgagcag
     2461 gaggacggcg tggcgcccac cgacagtcgc ctgcggccag accagcggct tatggagagg
     2521 ggacgctggg acgaggccaa caccgagaag caacggctgg aggagaagca gcgcctgtcc
     2581 aggcgacggc ggctggagtc atgcacggca ggctgcggtg gggaggaaga gaaggagtca
     2641 gatggctatg tgccgctctg gttcgagaag aggctggacc cgctgactgg ggagatggcc
     2701 tgcatgtaca agggcggcta ctgggaggcc aaggagaaga aggactggca catgtgcccc
     2761 aacatcttct gagccactcc tgcggcaaat acaggcgcct gcactgcctg ccagcctttt
     2821 ctttaatgca cccaatttac tacagaaccc acacacgctt tcgaaggggt aagggctgac
     2881 ctgggttctt gccagccccc agggtgtcgg gtcacctgtg cccgttatga ttgacttggg
     2941 ccccatgggc accctagttc ccagttccca taactcaggc cggctggtcg ggccatgggc
     3001 tgcccaggtc actaacccca ccccaccccc gctagggagt ctcttttgac ttttttaagg
     3061 aaaaaaaatc tccatttctt tccaacgatg aggtttagta aatatttttt agcgtatcac
     3121 ttaaaagact cactttccaa gtgtttgttc ttgccacata actatcctgg caagaagccc
     3181 ttttttaaag actcgaggaa gccaggcccc tttgaatggg gagcactttt aacacagcca
     3241 ggctttctat ctggactgtc caagctccag ggacgcaaca ccacggaggc agcaggaggc
     3301 ctggctgaca ccgcagcata gtgtgcagag cccgtgtggg ggggggggga acagaggaag
     3361 gtagaaggca aacatgcccc cccaccccgt gccaggtctt ggccatgctg ggacagcacc
     3421 ccaggcccag agacaccaag aagggctgaa aggcagggcc cttaggtgct tcctgcccaa
     3481 gaatcaggct cccaagtcag caataaggaa caaaccttca tttagcctgg gctggtgacc
     3541 cgggctcctg cacctggcct cttgctgtcc ttaggcggca tcagggtgcc tctcctcagc
     3601 actcctgaca tctttgtttg cctaatttat atatataata tacaaatata tataacataa
     3661 ctattttgct taaatttcta tgatacgtga aagtgaaaaa tgatgaagac aggagtgcct
     3721 gtctgagttt ggcctcctgt cggccctgcc ctcaccttgt agtcctccca gtggcttctg
     3781 ccaaccttag acctgaaacc tgtccctcag accctcagcc tccaacccat ttcacttcag
     3841 tttgatctgg aaagttcctt gcagggcctt tctttctact ccaggcctgg ttttgattgt
     3901 tttgttttgt tttttattta aaaaaagatt gagaaagaga aaacaccacg tatgaaatgc
     3961 cttacaacac ccactctaga gacggggtgg ggaaggagag ggagagggca cctggtctcc
     4021 gtcagagctg ggctgtacct cttcccagca ggcgtgagca aacaccttct gaccacaata
     4081 aaacccttgt tctggagttt tccaca
//
