LOCUS       NM_001171026            4049 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus amplified in osteosarcoma (Os9), transcript variant 1,
            mRNA.
ACCESSION   NM_001171026
VERSION     NM_001171026.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4049)
  AUTHORS   Hai,Q., Ritchey,B., Robinet,P., Alzayed,A.M., Brubaker,G., Zhang,J.
            and Smith,J.D.
  TITLE     Quantitative Trait Locus Mapping of Macrophage Cholesterol
            Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as
            a Causal Modifier Variant
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (1), 83-91 (2018)
   PUBMED   29097366
REFERENCE   4  (bases 1 to 4049)
  AUTHORS   Seaayfan,E., Defontaine,N., Demaretz,S., Zaarour,N. and Laghmani,K.
  TITLE     OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and
            Targets Its Immature Form for the Endoplasmic Reticulum-associated
            Degradation Pathway
  JOURNAL   J Biol Chem 291 (9), 4487-4502 (2016)
   PUBMED   26721884
  REMARK    GeneRIF: Data suggest renal cell lines exhibit an OS9-mediated ERAD
            (endoplasmic reticulum-associated degradation) pathway that
            degrades Nkcc2/Slc12a1 (solute carrier family 12 member 1) prior to
            glycosylation/processing.
REFERENCE   5  (bases 1 to 4049)
  AUTHORS   Maruyama,T., Dougan,S.K., Truttmann,M.C., Bilate,A.M., Ingram,J.R.
            and Ploegh,H.L.
  TITLE     Increasing the efficiency of precise genome editing with
            CRISPR-Cas9 by inhibition of nonhomologous end joining
  JOURNAL   Nat Biotechnol 33 (5), 538-542 (2015)
   PUBMED   25798939
  REMARK    Erratum:[Nat Biotechnol. 2016 Feb;34(2):210. doi:
            10.1038/nbt0216-210c. PMID: 26849523]
REFERENCE   6  (bases 1 to 4049)
  AUTHORS   Bernasconi,R., Pertel,T., Luban,J. and Molinari,M.
  TITLE     A dual task for the Xbp1-responsive OS-9 variants in the mammalian
            endoplasmic reticulum: inhibiting secretion of misfolded protein
            conformers and enhancing their disposal
  JOURNAL   J Biol Chem 283 (24), 16446-16454 (2008)
   PUBMED   18417469
REFERENCE   7  (bases 1 to 4049)
  AUTHORS   Wang,Y., Fu,X., Gaiser,S., Kottgen,M., Kramer-Zucker,A., Walz,G.
            and Wegierski,T.
  TITLE     OS-9 regulates the transit and polyubiquitination of TRPV4 in the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 282 (50), 36561-36570 (2007)
   PUBMED   17932042
  REMARK    GeneRIF: OS-9 regulates the secretory transport of TRPV4 and
            appears to protect TRPV4 subunits from the precocious
            ubiquitination and ER-associated degradation.
REFERENCE   8  (bases 1 to 4049)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   9  (bases 1 to 4049)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4049)
  AUTHORS   Nakayama,T., Yaoi,T., Kuwajima,G., Yoshie,O. and Sakata,T.
  TITLE     Ca2(+)-dependent interaction of N-copine, a member of the two C2
            domain protein family, with OS-9, the product of a gene frequently
            amplified in osteosarcoma
  JOURNAL   FEBS Lett 453 (1-2), 77-80 (1999)
   PUBMED   10403379
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131760.4, AK028047.1, BF470029.1, DV647242.1 and CD802565.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028047.1, SRR9219382.17016.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AC131760.4         41702-41730
            30-656              AK028047.1         5-631
            657-948             BF470029.1         25-316
            949-1225            AK028047.1         924-1200
            1226-1657           DV647242.1         70-501
            1658-1876           AK028047.1         1635-1853
            1877-1921           DV647242.1         556-600
            1922-2654           AK028047.1         1899-2631
            2655-2658           CD802565.1         17-20               c
            2659-4049           AC131760.4         67213-68603
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 74.5 cM"
     gene            1..4049
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /note="amplified in osteosarcoma"
                     /db_xref="GeneID:216440"
                     /db_xref="MGI:MGI:1924301"
     exon            1..204
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            205..381
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            382..445
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            446..522
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            523..621
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            622..832
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            833..934
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1029
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1084
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1170
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1467
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1657
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1822
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1935
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..4049
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2638..2643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2658
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /note="major polyA site"
     regulatory      4022..4027
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4049
                     /gene="Os9"
                     /gene_synonym="4632413K17Rik"
ORIGIN      
        1 tttggggcgg aagggacatc tctagcagag acggagcgga agatggcggc ggaggtgctg
       61 ctgtccagtc tgttgggatt gctatttcta gggctcctgt tacctgcgcg tctgacgggc
      121 ggtgtcggga gcctgaattt ggaggagctg agtgagatgc gttatggcat ccagatcttg
      181 ccgttgcctg tcatgggagg gcagagccaa gcttcagacg tggtggttgt gtcttcaaag
      241 tacaaacagc gctatgagtg ccgcctacca gctggagcta ttcacttcca acgtgaaaga
      301 gaggaggaga cgcctgctta ccaggggccc gggatccctg aattgttgag cccaatgaga
      361 gatgcccctt gtttgctgaa gaccaaggac tggtggacat atgaattctg ttatggacgt
      421 catatccagc agtaccatat ggaagactcg gagatcaaag gtgacgtcct ctatcttggc
      481 cactaccagt cctccttcaa ctgggacgac gaaacagcca aggcttccaa gcagcatcgg
      541 ctgaaacgct accacagcca gacctacggc aacgggtcca aatgtgatct caacgggaag
      601 ccccgagaag ctgaagttcg gttcctgtgt gacgagggtg cgggcatatc tggggactac
      661 attgaccgag tagatgaacc cgtctcctgc tcctacgtac tgaccattcg cacgtcaagg
      721 ctctgcccgc atcctctcct ccggccacca gccagcgctg ccccacaggc cattctttgt
      781 cacccagccc tgcagcctga tgagtacatg gcctacctcc agaggcaagc tgagtcaaag
      841 cagcatgaag agaaaaccac agaggaagtc caggacacag accgccaagt gtggagtggg
      901 agcaaggctg ccggagcacc cccaaagaaa gaagatgtca gcccagccaa ggaagagaag
      961 gaatcagagc tctggaagct tcaggggcca gaggagcagg cagcagcaag agaggaggcg
     1021 caggcagggg aacaggacct gaaccacgag gccgcagcag atccagctcc aagccctccc
     1081 aatgattttc agaataacgt gcaggtgaaa ctcatccgga gtcccgcaga cttgattcga
     1141 ctgattgagg agctgaaagc tgcagaaaag gggaagccaa gcgtaaggcg ggagcagcct
     1201 ggagacgata ccacggaggc cccccagagg gaagcagagg gaacgaaggc gaagggaaag
     1261 gatggtgagc ccccgggtct tatggaagag gaggatggtg acgacgaaga ggaggaggag
     1321 gaggaggaag aggacgagga ggagcagcag ctcctgggag agttcgagaa ggagctggag
     1381 gggatgttgc tgccctcaaa ccgcgagcgc ctccgctctg aggttaaggc tggcatggag
     1441 cgggagctgg agaacatcat ccaggagaca gagaaggaac tggacccaga agggctgagg
     1501 aaggagtccg agcgggagca ggcaatattg gctctaacat ccactctgga caaactcatc
     1561 aagaggctgc aggagaacca gagtccagag cttgtgcaaa aatacaaaaa aaggagagtt
     1621 gtcccccaaa agcctccccc atcaccacac cctacagagg aggaacctga gcacagagtc
     1681 cgggtccgag tcaccaagct ccgtcctgga ggccccaatc gggacctgac tgtcctggag
     1741 atgaaccggg aaaacccaca gctgaaacag atcgaggggc tggtgacaga agtgctggag
     1801 agggaggggc tcacggcgga aggcaagatt gagatcaaga ttgtgcgacc cggggctgaa
     1861 ggtaaggaag aggacacacg ctggctgact gatgaggata caagaaacct taaggagatt
     1921 ttcttcaata tcttggtaca gggagccgaa gaggccaata aagagcgcca gcgacagagt
     1981 gagctggaga gcaactaccg ccgggtgtgg ggctctcccg gtggtgaaga cacgggggac
     2041 ctggatgaat ttgacttctg agaccaccct gccagccctc taaggattcc tgcatcttcc
     2101 tgaccggccg gctctgcctc cttacacccc attctggggc cttggaggca aaagggcaga
     2161 ttggagccca gcactggact tgaggggccc agccccaaga gtgtatgagg gtgtgtgggg
     2221 acccatgagg gtgaggagct gttgcagcca ggcctatggg gtccactccc caaacacacc
     2281 ctcctttgcc cctttagctt tttcctgtcc cccaacttct tagcacttaa ccccactcta
     2341 cccagagact tgatgagacc ttgtgagtgg tggtggcagg aacagcaggg ctgtgcaggt
     2401 ccccgacccc acctctccct atcctacctc cattccctgt tgctgtttgg gagctgttgc
     2461 ccctcccttg gaacgcctac ttcctgtctc tgctgtctcc cggtacctgt gcttctgagc
     2521 ccagactatc tcttgtcact gaggggatgg gagacctttc atatcacctc acaaacaatc
     2581 ccagcaaaaa gggttggaga tggagcaccc ccttgttctt cagcaatcag gtttctaaat
     2641 aaaaaactgg accgtcaagc catcaggctc tgtggggagg gcaatcaggt cagagcagct
     2701 cagggtgccg atcctgcttg ctaaacgttt cccagaaaat gtcagtgtgc ctggggtaca
     2761 caggaggcga ccaggacagc tgaccaatgc cctacagtat ggagagatgc tgttttctcc
     2821 tgggtaagct gaccaggcac cagcctccag aggcctgggg gagagtcaca gtctctgctc
     2881 tgctctgctg tcatcttacg gacctctgaa aggataagga tttggagcca ggtctcagag
     2941 gaccctaaaa gccgtggcaa agctacagga gacctctggt ccctcctctc attatccatt
     3001 actatcccag gggctcacat gtatcaggtg agccctgtac tgctgagttg tgcccaacca
     3061 gtcacttcaa gcaaatcttt cctgatcctc tccttcctta cattctgaaa gccaaactgt
     3121 gtaccacaga gaccctccca cacccacctg gggcatcttc cttcctgcag catattccag
     3181 attccacctg ctagctaacc ccccctcccc cggattattt accattcctg gaaaactcca
     3241 cctacagtca tgaccaggct gctgcccaca agccattgtc acagtattcc agagacaggc
     3301 aggcctgact gccaaggcag ctgtgggttc ctcctgcccc tatgtcctct ctcaaggtca
     3361 tgggccctca ggacagtaaa gataaagttt ctcttttggg cccccagatg gagacaaaag
     3421 gggctcacta caggaaaggg actgaagaca acgtgccctg ccatctccag aagagtgaac
     3481 cgattatttc ggtaatgagg gcgagtgtcc cagtccttcc tcatctcaca aggcatcctc
     3541 tgagcaggat gcaaatgtgg aggcagtccc agaggatccc aaaactgctg ccaagttaca
     3601 ggagacaccc cccagaccga cagaggacga ggctgcagca gtggacttga gtcagtgcct
     3661 cagcgcagtt agaaaacaga aacctggctt cactgggtca gtctgaactg tcaacaatac
     3721 attccttttc cctctctctt gacttttggc aaaattccac gaagatcagt cagcctcagc
     3781 tatgccaagg caaacaaaag gaacagaacg gcaggtgtgt gtgttggggg tgggggtggg
     3841 ggtggggggg gcatggcatc cagaagaggg tggagaggtg cacggctgcc gaggaggggc
     3901 tgtgtgggca ctgaaggagc agcagcttgc ctgtgcctgt ccagggccac ccacccctcc
     3961 ttctcagccc cagtctaggg ggcttgaaat cttgtcattc cccacaaagc taatgtcacc
     4021 cattaaagta aatcaactag ttggcggta
//
