LOCUS       NM_011958               4056 bp    mRNA    linear   ROD 24-DEC-2022
DEFINITION  Mus musculus origin recognition complex, subunit 4 (Orc4),
            transcript variant 1, mRNA.
ACCESSION   NM_011958
VERSION     NM_011958.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Nguyen H, Wu H, Ung A, Yamazaki Y, Fogelgren B and Ward WS.
  TITLE     Deletion of Orc4 during oogenesis severely reduces polar body
            extrusion and blocks zygotic DNA replicationdagger
  JOURNAL   Biol Reprod 106 (4), 730-740 (2022)
   PUBMED   34977916
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Nguyen H, Ung A and Ward WS.
  TITLE     The role of ORC4 in enucleation of Murine Erythroleukemia (MEL)
            cells is similar to that in oocyte polar body extrusion
  JOURNAL   Syst Biol Reprod Med 66 (6), 378-386 (2020)
   PUBMED   32972244
  REMARK    GeneRIF: The role of ORC4 in enucleation of Murine Erythroleukemia
            (MEL) cells is similar to that in oocyte polar body extrusion.
REFERENCE   3  (bases 1 to 4056)
  AUTHORS   Nguyen H, James NG, Nguyen L, Nguyen TP, Vuong C, Ortega MA,
            Jameson DM and Ward WS.
  TITLE     Higher Order Oligomerization of the Licensing ORC4 Protein Is
            Required for Polar Body Extrusion in Murine Meiosis
  JOURNAL   J Cell Biochem 118 (9), 2941-2949 (2017)
   PUBMED   28230328
  REMARK    GeneRIF: Together, these data demonstrate that ORC4 oligomerization
            is required to form the ORC4 cage and that it is required for polar
            body extrusion.
REFERENCE   4  (bases 1 to 4056)
  AUTHORS   Nguyen H, Ortega MA, Ko M, Marh J and Ward WS.
  TITLE     ORC4 surrounds extruded chromatin in female meiosis
  JOURNAL   J Cell Biochem 116 (5), 778-786 (2015)
   PUBMED   25502171
  REMARK    GeneRIF: ORC4 surrounds the chromatin that will be extruded in the
            polar body in both female meiotic divisions, then makes a
            transition from the cytoplasm to the chromosomes at zygotic
            anaphase, suggesting multiple roles for this replication licensing
            protein.
REFERENCE   5  (bases 1 to 4056)
  AUTHORS   Camarena V, Cao L, Abad C, Abrams A, Toledo Y, Araki K, Araki M,
            Walz K and Young JI.
  TITLE     Disruption of Mbd5 in mice causes neuronal functional deficits and
            neurobehavioral abnormalities consistent with 2q23.1 microdeletion
            syndrome
  JOURNAL   EMBO Mol Med 6 (8), 1003-1015 (2014)
   PUBMED   25001218
REFERENCE   6  (bases 1 to 4056)
  AUTHORS   Kneissl M, Putter V, Szalay AA and Grummt F.
  TITLE     Interaction and assembly of murine pre-replicative complex proteins
            in yeast and mouse cells
  JOURNAL   J Mol Biol 327 (1), 111-128 (2003)
   PUBMED   12614612
REFERENCE   7  (bases 1 to 4056)
  AUTHORS   Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng
            EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC,
            Babbitt PC and Ferrin TE.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 4056)
  AUTHORS   Piao Y, Ko NT, Lim MK and Ko MS.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 4056)
  AUTHORS   Springer J, Kneissl M, Putter V and Grummt F.
  TITLE     Identification and characterization of MmORC4 and MmORC5, two
            subunits of the mouse origin of replication recognition complex
  JOURNAL   Chromosoma 108 (4), 243-249 (1999)
   PUBMED   10460412
REFERENCE   10 (bases 1 to 4056)
  AUTHORS   Tugal T, Zou-Yang XH, Gavin K, Pappin D, Canas B, Kobayashi R, Hunt
            T and Stillman B.
  TITLE     The Orc4p and Orc5p subunits of the Xenopus and human origin
            recognition complex are related to Orc1p and Cdc6p
  JOURNAL   J Biol Chem 273 (49), 32421-32429 (1998)
   PUBMED   9829972
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845521.9 and AL732317.13.
            
            [WARNING] On May 13, 2023 this sequence was replaced by
            NM_011958.5.
            
            On Sep 14, 2017 this sequence version replaced NM_011958.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1). Variants 1 and 4 both encode the same isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076324.1, AK084326.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-151               AL845521.9         2987-3137           c
            152-219             AL732317.13        216857-216924       c
            220-296             AL732317.13        213976-214052       c
            297-387             AL732317.13        213296-213386       c
            388-460             AL732317.13        210074-210146       c
            461-546             AL732317.13        209604-209689       c
            547-595             AL732317.13        206205-206253       c
            596-747             AL732317.13        203620-203771       c
            748-921             AL732317.13        193689-193862       c
            922-1008            AL732317.13        189086-189172       c
            1009-1117           AL732317.13        186710-186818       c
            1118-1213           AL732317.13        185943-186038       c
            1214-1281           AL732317.13        185790-185857       c
            1282-4056           AL732317.13        179349-182123       c
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..4056
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /note="origin recognition complex, subunit 4"
                     /db_xref="GeneID:26428"
                     /db_xref="MGI:MGI:1347043"
     exon            1..151
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            152..219
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..1470
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="origin recognition complex subunit 4 isoform 1"
                     /protein_id="NP_036088.3"
                     /db_xref="CCDS:CCDS16022.1"
                     /db_xref="GeneID:26428"
                     /db_xref="MGI:MGI:1347043"
                     /translation="MSSRKTKSNAHAECLSQVQRILRERFCHHSPHSNLFGVQVQYKH
                     LIELLKRTAIYGESNSVLIVGPRGSGKTTLLNHALKELMEIEVSENVIQVHLNGLLQT
                     NEKIALKEITRQLNLDNVVEDKVFGSFAENLSFLLEALQKGDRTSSCPVIFILDEFDI
                     FAHQKNQTLLYNLFDISQSAQTPVAVIGLTCRLDILELLEKRVKSRFSHRQIHLMNSF
                     DFPQYLKIFKEQLSLPAEFPDKAFAERWNENVHCLSEDSTVLEVLQKHFSVNKNLQSL
                     HMLLMLALNRVTVSHPFMTSADLMEAQHMCSLDSKANIVHGLSVLEICLIIAMKHLND
                     IYEEEPFNFQMVYNEFQKFIQRKAHSVYNFEKPVVMKAFEHLQQLELIKPVERTSVNS
                     QREYQLVKLLLDNTQIMNALQKYSNCPTDVRQWATSSLSWL"
     exon            220..296
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            297..387
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            388..460
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            461..546
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            547..595
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            596..747
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            748..921
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1008
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1117
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1213
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1281
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..4056
                     /gene="Orc4"
                     /gene_synonym="mMmORC4; Orc4l; Orc4P"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaagccggcc ggtcgccgtg catgctggga gttgctgtgc gcggtgggct gcagtaggag
       61 cagcgggact gagaaggtgg tgaacatcct tcggtcctca ccgtcggcgg cggccggctt
      121 tgcccgattt aggcaggctt gactgggaga ggatttgaat taatcgagat gagcagtcgt
      181 aaaaccaaga gtaatgcaca tgcagagtgt ctttcacagg tacaaagaat tttacgtgaa
      241 agattttgtc atcatagtcc acatagcaac ctctttggag tgcaagtaca atacaagcat
      301 ttgattgagc tactcaaaag aactgctatc tatggagaaa gcaattctgt actcattgtt
      361 ggaccacgag gatcaggaaa gaccacatta ttaaatcatg ctttgaaaga actcatggaa
      421 atagaagtga gtgaaaatgt gatacaagtt cacttaaatg gactcctgca gacaaatgaa
      481 aaaattgctc tgaaagagat cacaagacaa ttaaatctag acaatgtagt tgaagataaa
      541 gtttttggaa gctttgctga aaacctttca tttcttctgg aagctttgca aaaaggtgac
      601 cggactagca gttgcccagt gatcttcata ctggatgaat ttgatatttt tgctcatcag
      661 aaaaatcaaa cactccttta taatcttttt gacatttctc aatctgcaca gacaccagta
      721 gcagttattg gacttacatg tagattggat attttggaac tcttagaaaa gagagtgaag
      781 tcacgatttt ctcaccggca gatacattta atgaattcat ttgattttcc acaatatttg
      841 aaaatattta aagaacagtt atctctacct gcagaattcc cagataaggc ttttgctgag
      901 agatggaatg agaatgttca ctgtctctct gaagattcaa ctgtgcttga agtcctacag
      961 aaacatttca gtgtcaacaa aaacttgcag tcattacaca tgctattgat gcttgcttta
     1021 aatcgagtaa ccgtatcaca cccatttatg acttcagcag atctgatgga ggcacagcat
     1081 atgtgtagct tggattctaa ggcgaatatt gtacatggtc tgtcagtctt ggaaatctgt
     1141 cttataatag caatgaaaca tttaaatgac atatatgaag aggagccctt taattttcaa
     1201 atggtgtata atgaatttca gaaattcatt caaagaaagg cccattctgt ttataacttt
     1261 gagaaacctg tggtcatgaa ggcatttgag cacttacaac agttggaatt aataaaaccc
     1321 gtggaaagaa cttcagtaaa ttctcagaga gaataccagc tagtgaaact acttttggat
     1381 aatactcaaa ttatgaatgc tctacagaaa tactccaact gccctacaga tgttaggcag
     1441 tgggcaacat cctcactaag ctggctgtga agattttaag tacatttctg tgaagacctt
     1501 aaagtactgt tctttattta ataagatacg ctactacatt gctttgttta taaatagttt
     1561 atgggaaatt tgcacttagt atgttttgaa taatgaccac gcatggacac agtttcatta
     1621 atttaaattg caccacacaa gtagcagttc ataagaataa aatgtgctta ttttacgcat
     1681 ggtccataaa atgatttgat actcttttaa agtgtatgtg gtccattaga aaggttaatt
     1741 ccaacaaagc atttagttta cagatcataa tcctaagcag tcaggagaga aatacgaaca
     1801 gaaattacat gaggaatatt ccggctagat taggcgcctg tggatattgg agttgggttg
     1861 attttcctga attggttcct ataggatcct ccaattttgg atcttctaca gatcccccag
     1921 tgttctagtt tagtaaactg gcccatttgg gctgaagaac ttgtgtggaa agagtccaaa
     1981 caactcatca ttttcttctc tgcatatctc accacaattg taggattttt ttttccatgt
     2041 acattctttg tatatgttca gaagggatca aaattgccat ctaacaggta ccttaaagga
     2101 ttcctgggca tctacttcca caactaatac gtattccaca gtgttggctg ttggcttcaa
     2161 aaaaaggaat tctcttaaca tgctatgttg gtcagcgtcc acctcctaag ctcataggca
     2221 aaactcacag gttaaaatgg aatttaatct cagagatcct ttgttcaaga atatttgatt
     2281 gcatatttag ttgtagtaag attttataaa atacataatc ctttgtatgt gcagtttgta
     2341 aagtatctgc agaattaaat gctcttattt acagatcaga aaactaacac cagtgtcagg
     2401 atcacagtat gggaatgact gatgtgacct tgaatgcagc ttggtttgac tgacattaaa
     2461 gccagtgtta aaaactaaag cttagcaaca aatgttttac tatttttaca ggatactgta
     2521 aggaataatt tgacttttaa gaaatcttaa atataaattt tctgtgcatt aatgtagtac
     2581 ctcatagtta aaactttctt cattcacagc ctttcccttt cacagtaaaa catgcttagc
     2641 tttctgtgtc tttcttttag tcatgataaa aatgaagctg taatatcaag gtcatacaag
     2701 tataaacata tatgtccaat ttttaaacta cttccacctt acaagttgtt caaaatacat
     2761 tattagataa ttctaaagtg agaaaaatta gattctaaaa cacaaatcac aagaacgttt
     2821 gatattgaag tgtctggctt taacatgtga ttccacaaga gtagaagcaa gaaaggtata
     2881 tacttttagg agcctaaaaa acatttccaa actagcccaa gctatctcaa ctacaggagc
     2941 ggagggggac gaatgattgt gaacacaaag tctgacttga agattctctg caatgaagag
     3001 aattcattca ttcactctgc tcaagactcc actgggccaa gagatggaag aacaaacaaa
     3061 aaggactttc catgtagcct tcctccatgt ggcctagtga aacatggatc ttgtttaaaa
     3121 ggcatctgat tttatgcaga cacgtgtctg aaaaattaaa atccattgtc caaacttcca
     3181 tacattaggt aacgtcttat aatgtaagga catggtatcc actgccctaa agatttagac
     3241 atccatcaca gactctgcag tggtgtttct actccagtga ggtactttct accatggtgt
     3301 gccttttgct gtgagtgact taacagtgca ggttcttgtt gccttcactg ttaaccctga
     3361 gagcacataa tctgatgcat gtgtgactaa gaataagcct atggtcatgg gcaagaaggg
     3421 ccaacataga cacttgggta gaaatacagt ctgatcaagc aacggtgtaa ggaaaagtcc
     3481 ctaattcaca acctccttga tcttatgacc cttgaaaacc tcaagaccga agcctgacca
     3541 taagttggaa agctctcatt accctcttta acaacataaa ttcatagcta aagaaaaact
     3601 gcatgtgcaa ataataccat attcattttt tatttaacat ggggatggaa tggggatagc
     3661 atacatttta tttgtacaat atttaacaat cacttattca aggtgggatt ttatggggat
     3721 ttttttcatt tttagctttc tgttaaatac ttgcagaaaa aaaaaatagc aagtacaata
     3781 tgacagtagt acaacaacct gtgcccaaaa cactgacaag aaatacagag taaagctaaa
     3841 ataattgttt accaatattt tgagaggtaa caaataggga gatacgggtt gagactgcat
     3901 aaaaaacgta ctgctggttg aatatctgag gtaaatgatg tttaatcaag atgctacttg
     3961 tatctttgtc cataaattgt aaataatttt atgcacatta taccaaatct tcctaaaaag
     4021 taatttttca aaaaagtaaa aaagagaaac tggaaa
//
