LOCUS       NM_001318919            2999 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus opioid receptor-like 1 (Oprl1), transcript variant 1,
            mRNA.
ACCESSION   NM_001318919
VERSION     NM_001318919.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Morairty,S.R., Sun,Y., Toll,L., Bruchas,M.R. and Kilduff,T.S.
  TITLE     Activation of the nociceptin/orphanin-FQ receptor promotes NREM
            sleep and EEG slow wave activity
  JOURNAL   Proc Natl Acad Sci U S A 120 (13), e2214171120 (2023)
   PUBMED   36947514
REFERENCE   3  (bases 1 to 2999)
  AUTHORS   Camara,A.B. and Brandao,I.A.
  TITLE     Behavioral and neurochemical effects of nociceptin/orphanin FQ
            receptor activation in the social defeat protocol
  JOURNAL   Behav Neurosci 137 (1), 52-66 (2023)
   PUBMED   36326637
  REMARK    GeneRIF: Behavioral and neurochemical effects of
            nociceptin/orphanin FQ receptor activation in the social defeat
            protocol.
REFERENCE   4  (bases 1 to 2999)
  AUTHORS   Ke,X., Huang,Y., Fu,Q., Majnik,A., Sampath,V. and Lane,R.H.
  TITLE     Adverse maternal environment alters Oprl1 variant expression in
            mouse hippocampus
  JOURNAL   Anat Rec (Hoboken) 306 (1), 162-175 (2023)
   PUBMED   35983908
  REMARK    GeneRIF: Adverse maternal environment alters Oprl1 variant
            expression in mouse hippocampus.
REFERENCE   5  (bases 1 to 2999)
  AUTHORS   Loughran,G., Chou,M.Y., Ivanov,I.P., Jungreis,I., Kellis,M.,
            Kiran,A.M., Baranov,P.V. and Atkins,J.F.
  TITLE     Evidence of efficient stop codon readthrough in four mammalian
            genes
  JOURNAL   Nucleic Acids Res 42 (14), 8928-8938 (2014)
   PUBMED   25013167
REFERENCE   6  (bases 1 to 2999)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   7  (bases 1 to 2999)
  AUTHORS   Pan,Y.X., Xu,J., Wan,B.L., Zuckerman,A. and Pasternak,G.W.
  TITLE     Identification and differential regional expression of KOR-3/ORL-1
            gene splice variants in mouse brain
  JOURNAL   FEBS Lett 435 (1), 65-68 (1998)
   PUBMED   9755860
REFERENCE   8  (bases 1 to 2999)
  AUTHORS   Halford,W.P., Gebhardt,B.M. and Carr,D.J.
  TITLE     Functional role and sequence analysis of a lymphocyte orphan opioid
            receptor
  JOURNAL   J Neuroimmunol 59 (1-2), 91-101 (1995)
   PUBMED   7797625
REFERENCE   9  (bases 1 to 2999)
  AUTHORS   Pan,Y.X., Cheng,J., Xu,J., Rossi,G., Jacobson,E., Ryan-Moro,J.,
            Brooks,A.I., Dean,G.E., Standifer,K.M. and Pasternak,G.W.
  TITLE     Cloning and functional characterization through antisense mapping
            of a kappa 3-related opioid receptor
  JOURNAL   Mol Pharmacol 47 (6), 1180-1188 (1995)
   PUBMED   7603458
REFERENCE   10 (bases 1 to 2999)
  AUTHORS   Lachowicz,J.E., Shen,Y., Monsma,F.J. Jr. and Sibley,D.R.
  TITLE     Molecular cloning of a novel G protein-coupled receptor related to
            the opiate receptor family
  JOURNAL   J Neurochem 64 (1), 34-40 (1995)
   PUBMED   7798930
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY267034.1, BC050885.1 and
            AL845173.4.
            
            Summary: The protein encoded by this gene is a member of the 7
            transmembrane-spanning G protein-coupled receptor family, and
            functions as a receptor for the endogenous, opioid-related
            neuropeptide, nociceptin/orphanin FQ. This receptor-ligand system
            modulates a variety of biological functions and neurobehavior,
            including stress responses and anxiety behavior, learning and
            memory, locomotor activity, and inflammatory and immune responses.
            Alternatively spliced transcript variants have been described for
            this gene. A recent study provided evidence for translational
            readthrough in this gene, and expression of an additional
            C-terminally extended isoform via the use of an alternative
            in-frame translation termination codon. [provided by RefSeq, Dec
            2017].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes two isoforms, which result from the use of
            alternative in-frame translation termination codons. The shorter
            isoform (1) results from translation termination at the upstream
            UGA stop codon), while the longer isoform (1x) results from UGA
            stop codon readthrough to the downstream UAG termination codon.
            This RefSeq represents the longer, C-terminally extended isoform
            (1x). As the UGA stop codon has been reported to specify several
            alternative amino acids (tryptophan, cysteine, arginine and
            serine), the location of the upstream UGA stop codon in the longer
            isoform is denoted by an 'X'.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050885.1, AK079529.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            stop codon readthrough :: inferred from conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                BY267034.1         1-78
            79-2990             BC050885.1         1-2912
            2991-2999           AL845173.4         29588-29596
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 103.74 cM"
     gene            1..2999
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /note="opioid receptor-like 1"
                     /db_xref="GeneID:18389"
                     /db_xref="MGI:MGI:97440"
     exon            1..214
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            215..471
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             248..1438
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /note="isoform 1x is encoded by transcript variant 1;
                     nociceptin receptor; K3 opiate receptor; kappa-type 3
                     opioid receptor; N/OFQ receptor; nociceptin/ orphaninFQ
                     receptor"
                     /codon_start=1
                     /transl_except=(pos:1349..1351,aa:OTHER)
                     /product="nociceptin receptor isoform 1x"
                     /protein_id="NP_001305848.1"
                     /db_xref="CCDS:CCDS89611.1"
                     /db_xref="GeneID:18389"
                     /db_xref="MGI:MGI:97440"
                     /translation="MESLFPAPFWEVLYGSHFQGNLSLLNETVPHHLLLNASHSAFLP
                     LGLKVTIVGLYLAVCIGGLLGNCLVMYVILRHTKMKTATNIYIFNLALADTLVLLTLP
                     FQGTDILLGFWPFGNALCKTVIAIDYYNMFTSTFTLTAMSVDRYVAICHPIRALDVRT
                     SSKAQAVNVAIWALASVVGVPVAIMGSAQVEDEEIECLVEIPAPQDYWGPVFAICIFL
                     FSFIIPVLIISVCYSLMIRRLRGVRLLSGSREKDRNLRRITRLVLVVVAVFVGCWTPV
                     QVFVLVQGLGVQPGSETAVAILRFCTALGYVNSCLNPILYAFLDENFKACFRKFCCAS
                     ALHREMQVSDRVRSIAKDVGLGCKTSETVPRPAXLGVDLPMVPVSPQSPSTPNTELTQ
                     VTAL"
     exon            472..827
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..2999
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1352..1355
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /note="stop_codon_readthrough_signal"
                     /function="stimulates stop codon readthrough"
     regulatory      2966..2971
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /note="hexamer: AATAAA"
     polyA_site      2990
                     /gene="Oprl1"
                     /gene_synonym="KOR-3; KOR3; LC132; MOR-C; morc; NOP; Oprl;
                     ORGC; ORL1; XOR1"
                     /note="major polyA site"
ORIGIN      
        1 ttctgcccct cccccttctg gctgccgcac tggctgctgc gtctagtcaa tatcttatct
       61 tcggagcagg agctaggagc cattcccagc cggagcagac cccaagctag agtgagaagc
      121 attactcagt tcattgtgct cctgcctgcc tttctgctaa gcattagggt ctgttttggc
      181 ccagcttctg aagaggttgt gtgtgctgtt ggaggaactg tactgagtgg ctttgcaggg
      241 tgacagcatg gagtccctct ttcctgcccc attctgggag gtcttgtatg gcagccactt
      301 tcaagggaac ctgtctctcc taaatgagac cgtaccccat cacctgctcc tcaatgctag
      361 ccacagtgcc ttcctgcccc ttggactcaa ggtcaccatc gtggggctct acttggctgt
      421 gtgcatcggg gggctcctgg ggaactgcct cgtcatgtat gtcatcctca ggcacaccaa
      481 gatgaagact gctaccaaca tttacatatt taatctggca ctggctgata ccctggtctt
      541 gctgacactg cccttccagg gcacagacat ccttctgggc ttctggccat ttgggaatgc
      601 actgtgcaag acggtcattg ctatcgacta ctacaacatg tttaccagca ctttcacttt
      661 gactgccatg agtgtagacc gttatgtagc tatctgccac cctatccgtg cccttgatgt
      721 tcggacatcc agtaaagccc aggccgttaa tgtggccata tgggccctgg cttcggtggt
      781 tggtgttcct gttgccatca tgggctcagc acaagtggag gatgaagaga tcgagtgcct
      841 ggtggagatc cccgcccctc aggactattg gggccctgta tttgccatct gcatcttcct
      901 tttttccttc atcatcccgg ttctgatcat ctctgtctgc tacagcctca tgattcgacg
      961 acttcgtggt gtccggctgc tttcaggctc ccgagagaag gaccggaacc tgcgacgcat
     1021 cacacggctg gtactggtag ttgtggctgt gtttgtgggc tgctggacac ctgtgcaggt
     1081 ctttgtcctg gttcaaggac tgggtgttca gccaggtagt gagactgcag tagccattct
     1141 gcgcttctgc acagccctgg gctatgtcaa cagttgtctc aatcccattc tctatgcttt
     1201 cttggatgag aacttcaagg cctgctttag aaagttctgc tgtgcttctg ccctgcaccg
     1261 ggagatgcag gtttctgatc gtgtgcgcag cattgccaag gatgtaggcc ttggttgcaa
     1321 gacctctgag acagtaccac ggccggcatg actaggcgtg gacctgccca tggtgcctgt
     1381 cagtccacag agcccatcta cacccaacac ggagctcaca caggtcactg ctctctaggt
     1441 tgaccctgaa ctgagcgtct ggggccttga atggcttttc ttttggttca ggatgctcag
     1501 tcctagagga agacctttta gcaccatggg acaggtcaaa gcatcaaggt ggcctccatg
     1561 gcctctgtca gattaagttt cctccctggt ataggaccag agagaaccaa aggaactgca
     1621 tggaaacatc cacaactcag tggacatgcc tggtgaaccc atgtaggtat tcatggttca
     1681 cttgactctt ctctggtttc tccctgctgc cctggttcta ggtgggctca gcctgaggta
     1741 ttgtagttgt catgtagtca ctattgtgac tacctgttgt gtgctattgc cctcagcctt
     1801 tcagtgtttg cacagaactg gtgatcatac ccagtgttgc ctggccctta agcttggagt
     1861 tgccttggag catctagttc tgactccact gatgcattca gattacctga ggtgggtgag
     1921 catcagtggg ttcttggatg actgtttcct gacgattctt ttcatgctgt actatggagt
     1981 atatgaaggg gacttcacac ttcatctggt actgccactg cctgctctac caacctggac
     2041 caccttctca gcaagaggct agcaggggga caagacacaa agcttcccta aggctctttc
     2101 cctccaaaac cactgtgaac tcttattcta cagactgttt ggcaagccct gcttctaact
     2161 gtgtgggaag taatcaggag aaaattctgt ggcctctgta ggctgctcac agcatggagg
     2221 caccacatgc tggtcttggg tatgtgtctt ggctgctcag tatgggcagg gcagggcacg
     2281 agactatgct ctctccttat tctccacagc ctccctcagc tctccagcag tcgctctttt
     2341 acttgacagt agaggtcagc agttgtactg ctagaaacac acttgtagcc cgggaagact
     2401 ggagtcagga tgtgttctat tctataccca cagtgaccac ctgcttcatt tatagggtta
     2461 ggacatatcc aagcaaggcc tgggcttggc atcaaatgaa gagctggtat gagagctgaa
     2521 gcctaaaatg gctcatttga gccaatctgc aaggactatt acggttttgg ggacattgga
     2581 agaagagtcg ataccttgga gatatattgt tggttcacag aagaagaggc tttgtaaatg
     2641 ccctttctat gggtcagata aaaaaatacc agcaaccttg gatagaatct gatcttacac
     2701 agagaccttg gttaggtggt ttcatgccat gcagaaccca ggctggttcc tgtctcagag
     2761 agacctggcc ttaacaatct ttctggggag accagggttc tttcaatgac acttgtgcag
     2821 gagccattag ctctagtgtt ttcttgctaa tgctagaaac tttgtgaatt tgtatatctt
     2881 tatgtattta agcatgcaac aaatgtcatt ttcgtgcatg tagctagccc tgaacctgcc
     2941 tctggggtaa tgaaggagtt ctcataataa acacttacca gtgaccagta ctgcctgta
//
