LOCUS       NM_153122               4014 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus 5-oxoprolinase (ATP-hydrolysing) (Oplah), mRNA.
ACCESSION   NM_153122
VERSION     NM_153122.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   van der Pol,A., Gil,A., Tromp,J., Sillje,H.H.W., van
            Veldhuisen,D.J., Voors,A.A., Hoendermis,E.S., Grote Beverborg,N.,
            Schouten,E.M., de Boer,R.A., Bischoff,R. and van der Meer,P.
  TITLE     OPLAH ablation leads to accumulation of 5-oxoproline, oxidative
            stress, fibrosis, and elevated fillings pressures: a murine model
            for heart failure with a preserved ejection fraction
  JOURNAL   Cardiovasc Res 114 (14), 1871-1882 (2018)
   PUBMED   30032247
  REMARK    GeneRIF: Oplah full body knock-out mice had higher 5-oxoproline
            levels coupled to increased oxidative stress, myocardial fibrosis
            and elevated filling pressures reminiscent of heart failure with
            preserved ejection fraction.
REFERENCE   3  (bases 1 to 4014)
  AUTHORS   van der Pol,A., Gil,A., Sillje,H.H.W., Tromp,J., Ovchinnikova,E.S.,
            Vreeswijk-Baudoin,I., Hoes,M., Domian,I.J., van de Sluis,B., van
            Deursen,J.M., Voors,A.A., van Veldhuisen,D.J., van Gilst,W.H.,
            Berezikov,E., van der Harst,P., de Boer,R.A., Bischoff,R. and van
            der Meer,P.
  TITLE     Accumulation of 5-oxoproline in myocardial dysfunction and the
            protective effects of OPLAH
  JOURNAL   Sci Transl Med 9 (415) (2017)
   PUBMED   29118264
  REMARK    GeneRIF: OPLAH depletion occurred as a result of cardiac injury,
            leading to elevated 5-oxoproline and oxidative stress, whereas
            OPLAH overexpression improved cardiac function after ischemic
            injury.
REFERENCE   4  (bases 1 to 4014)
  AUTHORS   Duteil,D., Tosic,M., Lausecker,F., Nenseth,H.Z., Muller,J.M.,
            Urban,S., Willmann,D., Petroll,K., Messaddeq,N., Arrigoni,L.,
            Manke,T., Kornfeld,J.W., Bruning,J.C., Zagoriy,V., Meret,M.,
            Dengjel,J., Kanouni,T. and Schule,R.
  TITLE     Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose
            Tissue
  JOURNAL   Cell Rep 17 (4), 1008-1021 (2016)
   PUBMED   27760309
REFERENCE   5  (bases 1 to 4014)
  AUTHORS   Lu,W., Clasquin,M.F., Melamud,E., Amador-Noguez,D., Caudy,A.A. and
            Rabinowitz,J.D.
  TITLE     Metabolomic analysis via reversed-phase ion-pairing liquid
            chromatography coupled to a stand alone orbitrap mass spectrometer
  JOURNAL   Anal Chem 82 (8), 3212-3221 (2010)
   PUBMED   20349993
REFERENCE   6  (bases 1 to 4014)
  AUTHORS   Kumar,A. and Bachhawat,A.K.
  TITLE     A futile cycle, formed between two ATP-dependant gamma-glutamyl
            cycle enzymes, gamma-glutamyl cysteine synthetase and
            5-oxoprolinase: the cause of cellular ATP depletion in nephrotic
            cystinosis?
  JOURNAL   J Biosci 35 (1), 21-25 (2010)
   PUBMED   20413906
  REMARK    GeneRIF: the cause of cellular ATP depletion in nephrotic
            cystinosis may be the futile cycle, formed between two
            ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine
            synthetase and 5-oxoprolinase
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155074.12.
            
            On Jul 12, 2018 this sequence version replaced NM_153122.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034522.1, SRR17784650.158612.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                AC155074.12        140929-140990
            63-286              AC155074.12        141520-141743
            287-478             AC155074.12        141828-142019
            479-578             AC155074.12        142458-142557
            579-702             AC155074.12        142647-142770
            703-898             AC155074.12        142883-143078
            899-1064            AC155074.12        143160-143325
            1065-1203           AC155074.12        143446-143584
            1204-1271           AC155074.12        143685-143752
            1272-1537           AC155074.12        143866-144131
            1538-1650           AC155074.12        144670-144782
            1651-1821           AC155074.12        144870-145040
            1822-1959           AC155074.12        145225-145362
            1960-2098           AC155074.12        145449-145587
            2099-2211           AC155074.12        145724-145836
            2212-2363           AC155074.12        146021-146172
            2364-2524           AC155074.12        146771-146931
            2525-2626           AC155074.12        147024-147125
            2627-2801           AC155074.12        147218-147392
            2802-2986           AC155074.12        149647-149831
            2987-3133           AC155074.12        149936-150082
            3134-3268           AC155074.12        150178-150312
            3269-3418           AC155074.12        150450-150599
            3419-3576           AC155074.12        150702-150859
            3577-3737           AC155074.12        150941-151101
            3738-3835           AC155074.12        151200-151297
            3836-4014           AC155074.12        151437-151615
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 35.67 cM"
     gene            1..4014
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /note="5-oxoprolinase (ATP-hydrolysing)"
                     /db_xref="GeneID:75475"
                     /db_xref="MGI:MGI:1922725"
     exon            1..62
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            63..286
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..3982
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /EC_number="3.5.2.9"
                     /note="pyroglutamase; 5-oxo-L-prolinase"
                     /codon_start=1
                     /product="5-oxoprolinase"
                     /protein_id="NP_694762.1"
                     /db_xref="CCDS:CCDS27565.1"
                     /db_xref="GeneID:75475"
                     /db_xref="MGI:MGI:1922725"
                     /translation="MGSPEERFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYA
                     DAPTEGIRRILEQERGVLLPRGRPLDTSHIASIRMGTTVATNALLERQGERVALLVTR
                     GFRDLLHIGTQARPDLFDLAVPMPEVLYEEVVEVDERVLLYRGEPGAGSPVKGCTGDL
                     LEIQQPVDLAALRGKLEGLLTRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSL
                     SSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAP
                     MDAFSGSRAVLSGPAGGVVGYSTTTYQLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFE
                     ASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVT
                     DANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLASGPCPASQLSL
                     EEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTV
                     HIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQAQG
                     FSRSQISTESFLHLRYQGTDCALMVSANQHPATTCSPRAGDFGAAFVERYMREFGFII
                     PERSVVVDDVRVRGTGRSGLQLEETSKIQSGPPHVEKVTQCYFEGGYQETPVYLLGEL
                     GYGHQLQGPCLIIDNNSTILVEPGCQAEVIETGDIRISVGAEAPSMIDTKLDPIQLSI
                     FSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQE
                     TVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQSRPVFYVASRGHH
                     ADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALQAPGKISGCSGTR
                     NLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFG
                     TSRQARGLPLEVSAKDHMDDGSPICLHVQINLNQGSAVFDFSGSGSEVFGNLNAPRAI
                     TLSALIYCLRCLVGRDIPLNQGCLAPVQVIIPKGSILDPSPEAAVVGGNVLTSQRVVD
                     VILGAFGACAASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRIT
                     DPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPY
                     GLHGGEPGTRGLNLLIRKDGRTVNLGGKTSVTVYPGDAFCLHTPGGGGYGDPEDPAPP
                     PGSPPLYPAFPERGSVYEYRRAQEAV"
     exon            287..478
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            479..578
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            579..702
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            703..898
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1064
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1203
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1271
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1537
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1650
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1821
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..1959
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2098
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2211
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            2212..2363
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            2364..2524
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2626
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            2627..2801
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2986
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            2987..3133
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3268
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            3269..3418
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            3419..3576
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            3577..3737
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            3738..3835
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     exon            3836..4014
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3993..3998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /note="hexamer: AATAAA"
     polyA_site      4014
                     /gene="Oplah"
                     /gene_synonym="1700010G02Rik; 5-OPase"
                     /note="major polyA site"
ORIGIN      
        1 agggccttcg ggaagcgcag cgggacacaa gcaagactag agcggattcg gctgctggtg
       61 ggttctccca gggctaccag ccgaagagcc actgctggac tccagcttca acatcatggg
      121 cagcccagaa gagcgcttcc attttgccat cgaccgtggt ggcaccttta cagatgtctt
      181 tgcccagtgc ccaggagggc atgtacgtgt cctgaagctg ctctcagagg atcctgccaa
      241 ctatgcagac gcacccacag agggcatccg ccgaattcta gagcaggaga ggggtgtgct
      301 gctgccccga ggccggccgc tagacaccag tcacatcgcc agcatccgca tgggtaccac
      361 cgtggccacc aatgctctgt tggaacggca gggagaacgg gtggcactgc tggtgactcg
      421 cggtttccga gacctgctgc atattggcac tcaggcccgc ccagacctct ttgacctggc
      481 tgtgcccatg ccagaggtac tgtatgagga ggtcgtggag gtggacgagc gagtgctgct
      541 gtatcgtgga gaacccggtg ccggctctcc cgtcaaaggc tgtacagggg acctgctaga
      601 gatacagcag cctgtggatc tggcagccct gcgtgggaag ctggaggggc tcttgactcg
      661 gggcattcac agcctggcag tggtactcat gcattcgtac acgtgggccc agcacgagca
      721 gcaggtgggc acactggccc gggagctggg cttcacgcac gtctccttgt cctcggaagt
      781 catgcccatg gtgcggattg ttcctcgggg gcatacggcc tgtgcagatg cttacctcac
      841 tcccaccatc cagcgctatg tgcagggctt ccgccgaggc ttccagggcc agctaaagaa
      901 tgtgcaagtc ctcttcatgc gctccgatgg tggcctggca cccatggatg ccttcagtgg
      961 ctcccgggct gtgctctctg gccctgctgg tggtgtggtt ggctactcca ctaccaccta
     1021 ccagctggaa ggtggtcagc ctgtcattgg ctttgacatg ggaggcacat ccacagatgt
     1081 gagccgctat gctggagagt ttgagcacgt ctttgaggct agcacggcag gtgttaccct
     1141 ccaggcccca cagctggaca tcaatacggt ggcagctggc gggggttccc gcctcttctt
     1201 caggtctggc ctctttgtgg ttggtccaga gtcagcaggt gcccacccag gtcctgcctg
     1261 ctaccgcaaa gggggtcctg tgacagtgac agatgctaat ctggttctgg gtcgcctgct
     1321 gcctgcctcc ttcccctgca tttttgggcc aggagaagac cagccattgt ctcccgaggc
     1381 ttcccgaaag gctctagagg ctgtggccat ggaggtcaac agtttcttgg ccagtggacc
     1441 ctgcccagct tcccagctaa gtctggagga ggtggccatg gggtttgtgc gtgttgccaa
     1501 cgaagccatg tgccggccta tccgtgccct cacacaggca cgaggccacg acccctcagc
     1561 ccatgtactg gcttgctttg gaggagccgg tgggcaacac gcttgtgcca ttgcccgggc
     1621 cctgggtatg gacactgtgc atattcacag gcacagcggg ctgctgtcag cactgggact
     1681 ggccttggcc gatgtggttc acgaagcaca ggagccctgt tccctgtctt acacacctga
     1741 aacctttgca caactggacc agagactgag ccgcctggag gagcagtgtg tggatgccct
     1801 gcaggcccag ggcttctcta ggtctcagat cagcaccgag agcttcctgc accttcgcta
     1861 ccaaggcact gactgtgccc taatggtgtc tgccaatcag catccggcca caacctgctc
     1921 accccgtgct ggtgactttg gagcagcgtt tgtcgagagg tacatgagag agtttggctt
     1981 catcattcct gagcgctcgg tggtggtaga tgatgtgcgt gtgaggggaa ctggccgtag
     2041 tggacttcag ctggaggaga cctccaaaat ccagagtgga cctccgcatg tggaaaaggt
     2101 gacccagtgc tactttgaag ggggttatca ggagactccc gtgtaccttt taggagaact
     2161 aggctacggg catcagcttc aagggccctg cctgatcatt gacaacaaca gcaccatcct
     2221 tgtagaacca ggttgccaag cggaggtgat tgagacaggg gacatccgca tttctgtggg
     2281 agccgaggct cccagtatga tagacaccaa gcttgacccc atccagttgt ctattttctc
     2341 acaccgtttc atgagcattg ctgagcagat gggccgcatc ctacagcgca cagccatctc
     2401 taccaacatc aaggaacgcc tcgacttctc ctgtgccctc tttgggccag atgggggcct
     2461 cgtctccaat gctccccaca ttcctgtgca cctgggtgcc atgcaagaga ctgtacagtt
     2521 ccagattcag cacttaggag ccgacctcca tcctggtgat gtgttgctta gtaaccatcc
     2581 cagtgcaggg ggcagccatc ttcctgacct gactgtcatc acaccggtgt tttggccagg
     2641 tcagtcgagg cctgtgttct acgtggctag ccgagggcac cacgcggaca ttggaggtat
     2701 caccccgggt tctatgcctc ctcactctac cacactgcag caggagggtg ccgtttttct
     2761 gtccttcaaa ctggtccagg gaggagtctt ccaggaagag gcggtgacag aggccttaca
     2821 ggccccaggc aagatctctg gctgcagtgg gaccaggaac ctgcatgaca acctgtcgga
     2881 tctccgtgcc caggtggcag ctaaccagaa aggcatccag ctggtgggag agctgatcgg
     2941 acagtatggc ttagatgtgg tgcaggccta tatgggccat attcaggcaa atgctgaact
     3001 agcagtgcga gacatgcttc gtgcttttgg aacttcccgc caggccaggg gcctgcccct
     3061 ggaggtgtct gccaaggatc acatggacga tggctctccc atctgcctgc atgttcagat
     3121 caacctgaat cagggcagcg ctgtatttga cttcagtggt tctgggtctg aggtgtttgg
     3181 caatctcaat gcccctcgag ccataacact gtctgctctc atctattgct tgcgctgtct
     3241 agtgggccgt gacatcccac ttaaccaggg ttgcctggct cctgtgcaag tcataattcc
     3301 caaaggctcc atattggatc catccccaga ggcagcagtg gtcggaggca acgtgctcac
     3361 atctcagcga gtcgtggatg tcatcctggg ggcttttggg gcctgtgcag cctcccaggg
     3421 ctgcatgaac aatgtgaccc tgggcaatgc ccgtatgggc tactatgaga cagtagctgg
     3481 tggtgcgggt gcaggccctg gctggcatgg gcgcagtggt gtacacagtc acatgaccaa
     3541 cacgcgcatt acggacccag agattttgga gagccggtat ccagttatcc tgcgccgctt
     3601 tgagctgagg ccaggctctg gaggccgagg ccgcttccgg ggaggtgatg gcgtagtccg
     3661 agagctggtc tttcgggaag aggcgctact gtctgtcctc acggagcgcc gcgccttcca
     3721 gccttatggc ctccacgggg gagagcctgg tacacgtggc ttaaacctcc tgatcagaaa
     3781 agatgggcgc acagtgaatc tgggcggcaa gacatccgtg actgtgtatc ccggggacgc
     3841 gttctgcctc cacacgcctg ggggtggggg ctatggagac ccggaggacc cagcaccacc
     3901 accaggctcg cccccgctat atccagcctt cccagagcga ggcagtgtgt atgagtaccg
     3961 ccgcgcccag gaagcagtat gagtgccccc acaataaaga tcctttgagt cgca
//
