LOCUS       NM_052976               7216 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus oligophrenin 1 (Ophn1), transcript variant 1, mRNA.
ACCESSION   NM_052976
VERSION     NM_052976.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Cresto,N., Lebrun,N., Dumont,F., Letourneur,F., Billuart,P. and
            Rouach,N.
  TITLE     Hippocampal Excitatory Synaptic Transmission and Plasticity Are
            Differentially Altered during Postnatal Development by Loss of the
            X-Linked Intellectual Disability Protein Oligophrenin-1
  JOURNAL   Cells 11 (9), 1545 (2022)
   PUBMED   35563851
  REMARK    GeneRIF: Hippocampal Excitatory Synaptic Transmission and
            Plasticity Are Differentially Altered during Postnatal Development
            by Loss of the X-Linked Intellectual Disability Protein
            Oligophrenin-1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Liu,J., Zhang,Y., Li,S., Sun,F., Wang,G., Wei,D., Yang,T. and Gu,S.
  TITLE     Androgen deprivation-induced OPHN1 amplification promotes
            castration-resistant prostate cancer
  JOURNAL   Oncol Rep 47 (1) (2022)
   PUBMED   34738630
  REMARK    GeneRIF: Androgen deprivationinduced OPHN1 amplification promotes
            castrationresistant prostate cancer.
REFERENCE   3  (bases 1 to 7216)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7216)
  AUTHORS   Wang,M., Gallo,N.B., Tai,Y., Li,B. and Van Aelst,L.
  TITLE     Oligophrenin-1 moderates behavioral responses to stress by
            regulating parvalbumin interneuron activity in the medial
            prefrontal cortex
  JOURNAL   Neuron 109 (10), 1636-1656 (2021)
   PUBMED   33831348
  REMARK    GeneRIF: Oligophrenin-1 moderates behavioral responses to stress by
            regulating parvalbumin interneuron activity in the medial
            prefrontal cortex.
REFERENCE   5  (bases 1 to 7216)
  AUTHORS   Maset,A., Galla,L., Francia,S., Cozzolino,O., Capasso,P.,
            Goisis,R.C., Losi,G., Lombardo,A., Ratto,G.M. and Lodovichi,C.
  TITLE     Altered Cl- homeostasis hinders forebrain GABAergic interneuron
            migration in a mouse model of intellectual disability
  JOURNAL   Proc Natl Acad Sci U S A 118 (2) (2021)
   PUBMED   33376209
  REMARK    GeneRIF: Altered Cl(-) homeostasis hinders forebrain GABAergic
            interneuron migration in a mouse model of intellectual disability.
REFERENCE   6  (bases 1 to 7216)
  AUTHORS   Khelfaoui,M., Pavlowsky,A., Powell,A.D., Valnegri,P., Cheong,K.W.,
            Blandin,Y., Passafaro,M., Jefferys,J.G., Chelly,J. and Billuart,P.
  TITLE     Inhibition of RhoA pathway rescues the endocytosis defects in
            Oligophrenin1 mouse model of mental retardation
  JOURNAL   Hum Mol Genet 18 (14), 2575-2583 (2009)
   PUBMED   19401298
  REMARK    GeneRIF: Loss of OPHN1 leads to the activation of the RhoA/ROCK
            signaling pathway.
REFERENCE   7  (bases 1 to 7216)
  AUTHORS   Nakano-Kobayashi,A., Kasri,N.N., Newey,S.E. and Van Aelst,L.
  TITLE     The Rho-linked mental retardation protein OPHN1 controls synaptic
            vesicle endocytosis via endophilin A1
  JOURNAL   Curr Biol 19 (13), 1133-1139 (2009)
   PUBMED   19481455
  REMARK    GeneRIF: Results sugest that reduced or defective OPHN1 signaling
            impairs synaptic vesicle cycling at hippocampal synapses.
REFERENCE   8  (bases 1 to 7216)
  AUTHORS   Vaillend,C., Poirier,R. and Laroche,S.
  TITLE     Genes, plasticity and mental retardation
  JOURNAL   Behav Brain Res 192 (1), 88-105 (2008)
   PUBMED   18329113
  REMARK    Review article
REFERENCE   9  (bases 1 to 7216)
  AUTHORS   Khelfaoui,M., Denis,C., van Galen,E., de Bock,F., Schmitt,A.,
            Houbron,C., Morice,E., Giros,B., Ramakers,G., Fagni,L., Chelly,J.,
            Nosten-Bertrand,M. and Billuart,P.
  TITLE     Loss of X-linked mental retardation gene oligophrenin1 in mice
            impairs spatial memory and leads to ventricular enlargement and
            dendritic spine immaturity
  JOURNAL   J Neurosci 27 (35), 9439-9450 (2007)
   PUBMED   17728457
  REMARK    GeneRIF: This study indicates that cognitive impairment related to
            OPHN1 loss of function is associated with both presynaptic and
            postsynaptic alterations.
REFERENCE   10 (bases 1 to 7216)
  AUTHORS   Fauchereau,F., Herbrand,U., Chafey,P., Eberth,A., Koulakoff,A.,
            Vinet,M.C., Ahmadian,M.R., Chelly,J. and Billuart,P.
  TITLE     The RhoGAP activity of OPHN1, a new F-actin-binding protein, is
            negatively controlled by its amino-terminal domain
  JOURNAL   Mol Cell Neurosci 23 (4), 574-586 (2003)
   PUBMED   12932438
  REMARK    GeneRIF: Oligophrenin-1 is expressed in developing CNS areas that
            evince synaptic plasticity. OPHN1 is expressed in both glial and
            neuronal cells, where it colocalizes with actin at the tip of
            growing neurites.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK039304.1, AK036038.1, AL831715.7 and BE136101.1.
            
            On Sep 1, 2015 this sequence version replaced NM_052976.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Variants 1 and 2
            both encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK036038.1, AK031419.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AK039304.1         2-41
            41-3972             AK036038.1         2-3933
            3973-6820           AL831715.7         63795-66642         c
            6821-7216           BE136101.1         1-396               c
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 42.94 cM"
     gene            1..7216
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /note="oligophrenin 1"
                     /db_xref="GeneID:94190"
                     /db_xref="MGI:MGI:2151070"
     exon            1..645
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            646..818
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     CDS             665..3073
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /note="isoform a is encoded by transcript variant 1"
                     /codon_start=1
                     /product="oligophrenin-1 isoform a"
                     /protein_id="NP_443208.1"
                     /db_xref="CCDS:CCDS30295.1"
                     /db_xref="GeneID:94190"
                     /db_xref="MGI:MGI:2151070"
                     /translation="MGHPPLEFSDCYLDSPDFRQRLKYYEEELERTNKFIKDVIKDGS
                     ALISAMRNYSSAVQKFSQTLQSFQFDFIGDTLTDDEINIAESFKEFAELLNEVENERM
                     MMVQNASDLLIKPLETFRKEQIGFTKERKKKFEKDGERFYSLLDRHLHLSSKKKESQL
                     LEADLQVDKERHNFFESSLDYVYQIQEVQESKKFNIVEPVLAFLHSLFISNSLTVELT
                     QDFLPYKQQLQLSLQNTRNHFSSTREEMEELKKRMKEAPQTCKLPGQPTIEGYLYTQE
                     KWALGISWAKYYCRYEKETRMLTMIPMEQKPGAKQGPVDLTLKYCVRRKTESIDKRFC
                     FDIETNERPGTITLQAPSEANRRLWMEAMDGKEPIYHTPITKQEEMELNEVGFKFVRK
                     CINFIETKGIKTEGLYRTVGSNIQVQKLLYAFFDPKCPGDVDFHNSDWDIKTITSSLK
                     FYLRNLSEPVMTYKLHKELVSAAKSDNLDYRLGAIHSLVYKLPEKNREMLELLIKHLV
                     NVCEHSKENLMTPSNMGVIFGPTLMRAQEDTVAAMMNIKFQNIVVEILIEHFGKIYLG
                     PPEDSQVPPVPPPRVTARRHKPITISKRLLREKTVFYTSSLDENKDESHHQTPNGTIT
                     SNLDPPKLLQHLKPPMQKSGETDPGRKSPSRPVSDCQSEPCLETDVGRLLFRLQDGGT
                     KATPKASNGPVPGSGHTKTSSFHIRRPAPRPMAHHKEGDTDGFSKVRPPGEKQTIIRP
                     PVRPPDPPCRSITPQKPEPKPETGSGNADEIPSSVVASRTRFFETASRKTGSSQGKLP
                     GDES"
     exon            819..914
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            915..976
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1048
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1150
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1261
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1366
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1496
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1597
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1689
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1768
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1802
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1865
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1940
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2025
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2084
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2190
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2350
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            2351..2498
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            2499..2822
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            2823..2988
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3039
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            3040..3081
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..7207
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3208..3213
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /note="hexamer: TATAAA"
     polyA_site      3232
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /note="major polyA site"
     regulatory      4160..4165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /note="hexamer: AATAAA"
     polyA_site      4179
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
     regulatory      5907..5912
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /note="hexamer: AATAAA"
     polyA_site      5927
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
     regulatory      7189..7194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
                     /note="hexamer: ATTAAA"
     polyA_site      7207
                     /gene="Ophn1"
                     /gene_synonym="C130037N19Rik; Wtgr"
ORIGIN      
        1 acgctttcca gatggagtcc tagggcgctg actgctcccc agtttccgta gggaagcgcc
       61 gggctaccac agctattgtg cgtagcgttg tcgccctcct cttcccgctg cctgctgctg
      121 tgttgttgga ggaaagcaac gtttccttgc cgccgggctt aactctctaa gtgccaaacc
      181 ccacatcacg atggctcccg ggaaccagta ggcggtctct gcagcttccg agttgtgggc
      241 gacgagccct gaagacccga ggaagacatc caaagggatc ctgtgctctc gttcggtctt
      301 ggggttaggg ctcggccagc gtgaaaaggt ggcattggct ttaagaaggg ggaggggagg
      361 cacagtctgc ctctcttggg gagccgggga aggaagcctt aggctggctt gggagcgcaa
      421 agaggcggcc cccgcgcccg tgctctcccc gcctctaccc tgggacgccc gggacacagc
      481 gttgtgcttt ggggagacga tgaccagggg gcgtgctgct ccctaggctg caccctgccg
      541 gcgtcagaag acgttggtaa gcactcctgg tcacccgcag ccaccaccgg actacgcgct
      601 ccagctacac accagcgggc gctcccaaga gtttcattgg aacagcccct ccgctatcct
      661 aatcatgggg catcccccgc tggagttcag cgactgctac ctcgacagcc ccgatttccg
      721 ccagaggctc aagtattatg aagaggagct ggagaggacc aataaattca tcaaagacgt
      781 gatcaaagac ggcagcgcgc ttatcagcgc tatgagaaat tactcttctg cggttcagaa
      841 attttctcag acactccagt catttcaatt tgatttcatt ggagacacgc tgaccgatga
      901 tgagattaat atagcggaat cattcaagga atttgctgaa ttgctcaatg aagtagaaaa
      961 tgaaaggatg atgatggtac aaaatgctag tgacttgctg atcaaacccc tggaaacttt
     1021 tcggaaggaa caaataggtt ttacaaagga aaggaagaag aaatttgaaa aggatggtga
     1081 gaggttttac tcgctactgg accgccactt acatctgtca tccaaaaaga aagaatctca
     1141 gctactagag gcagacctcc aagtggataa ggaaaggcac aatttctttg aatcatctct
     1201 tgactatgtt tatcaaattc aggaagtcca ggagtcaaag aagttcaata ttgtggagcc
     1261 ggtcttggcc ttccttcata gcctcttcat ttctaacagc ttgactgtgg agctcacaca
     1321 ggatttcctg ccatacaaac aacagctgca gctcagctta cagaatacaa gaaatcattt
     1381 ctccagtacc cgggaggaga tggaagaact taagaaaagg atgaaagaag cccctcagac
     1441 atgcaaactt ccaggacagc caaccattga aggctatctc tacacacagg agaaatgggc
     1501 cctggggata tcctgggcga aatactattg tcggtatgag aaagagacca ggatgctcac
     1561 aatgatacca atggaacaga agccaggtgc taagcaggga ccggtggact taactttgaa
     1621 gtactgcgtg agaaggaaga cagagtcaat tgacaagaga ttctgttttg atatagaaac
     1681 taatgaaaga cctggaacca tcactctgca ggcgccttcc gaagccaata gaaggctatg
     1741 gatggaagcc atggatggca aagaacctat ctaccacact cctataacaa aacaggaaga
     1801 aatggagctg aatgaagtgg ggttcaaatt tgttaggaag tgcatcaatt ttattgagac
     1861 caaagggatc aagacagaag ggctgtaccg caccgtgggc agcaacatcc aggttcagaa
     1921 gctgctctat gccttttttg atccaaaatg tccaggggat gttgatttcc ataacagtga
     1981 ctgggacatt aagacaatca caagctcttt aaaattctac ctcaggaatc tctcagaacc
     2041 tgtcatgact tataagctcc ataaagaact cgtctctgct gccaaatctg acaacctgga
     2101 ttaccgtctg ggggctattc actccctagt atataagcta ccagaaaaga accgggagat
     2161 gctggaactt ctaatcaaac acttggtaaa tgtgtgtgag cacagcaaag agaatcttat
     2221 gacaccttcc aatatggggg tgatctttgg gcccaccctg atgagagctc aagaggacac
     2281 tgtggctgct atgatgaaca tcaaattcca gaacattgtg gtagaaatcc tgattgaaca
     2341 ctttggcaag atttatttag gtccacccga agatagccaa gtacctccag tgcctccacc
     2401 tcgagtgaca gcaagaagac acaaaccaat cacaatctca aagcgcttgc tgagagaaaa
     2461 gactgtgttc tatacttctt ccctggatga aaataaagat gaaagtcacc atcaaactcc
     2521 taatggtaca atcactagca acctggatcc ccccaagcta ctgcagcacc tcaaaccacc
     2581 catgcaaaaa agtggggaaa ctgaccctgg aagaaagtcc ccaagcagac ctgtttctga
     2641 ctgtcaatcg gagccctgcc tagagacgga tgtggggagg ttgttattta ggttgcagga
     2701 tggagggacc aaggctactc caaaggcttc caatggacct gttccaggct ctggacacac
     2761 aaagacctcc tctttccata taaggagacc agctcccagg ccaatggctc accacaagga
     2821 aggcgatact gacggtttca gcaaagtccg gcccccagga gaaaagcaaa caatcatccg
     2881 tcctccggtg aggcccccag accctccttg ccggtccatc actccacaaa agccagagcc
     2941 aaagccagaa acagggtctg gcaatgcaga cgaaatccca tcatccgtgg tggcatctag
     3001 gaccagattc tttgaaacag cttcccggaa aactggaagt tctcaaggaa aacttcctgg
     3061 agatgagagt tgaggccaca ggtttgaaag gacttggcct tgagggaccc ttttttggat
     3121 gggaaagggt ctacttcaaa cccttgtgag ctattggtgc tgaagagcag ctctttctgg
     3181 ccttgtaggg ttcagtgtct gggaacctat aaaataagga acacatgtct taggcacgtg
     3241 tttgtttctc ctttgtattc tgtcttcatc tcctcagaat ttctgttgtg agcaattcct
     3301 aggacactgg atggctggat tctctcaaac tccaagctct actgaaggga aaatacccac
     3361 tgatctgtat tgaagaatag agcctagttt cccaatcttc tctgaacagc ttgtttccgc
     3421 aaacgtcatt ggattctgca tgagtcaggt ctaaagactg ttcttagggg gtcttactct
     3481 gatctaaagg gttggggaca ttgtttacct tatttggaat aggggtaggg atgttagctg
     3541 aatattggag aattgtatca tgtctctttc caaggatgtg gatgctgact aattccagat
     3601 tggtgccctg gtacatgtta ctgaaatcca gaggggctaa accgcatgaa taattcccac
     3661 ccagtgtccg tccccagtat gggtttagga agatagactt ttgtctcttt gcttccagcc
     3721 tctgaggaca aatctgctaa gcatgttttg catgtcaaag cctaggtctg tctggatttt
     3781 ccttcttcct actttctcgc agtaacttcc ttttcagcaa tcaacaaata aaacagaaca
     3841 gaaaacattt ctgttaaaaa aaaaaaagag ggggtcaaat gttaggaaaa attaagcgtc
     3901 caaagaattg ttgctcttgc ttctatttac ctccagctta catggtcttt tatcccaccc
     3961 cccacccccg ccattaagaa aaaaaaaaaa acaagcaaac aaagccacaa acaaatgaaa
     4021 ccagatctta accaggtgct tatggattct ggaactccat gatgccccta agatgacata
     4081 tacaattcca tgagggtaat gccttttctg agcagatggt tcataacatt ctactgaatt
     4141 tcaaatggga ctgcaaccca ataaaggtta agaaccgcag cctttgagat aaaagactct
     4201 ttacagccca taaagggttg gattagaagg aactttcaaa agcatttata ctaacaacag
     4261 cttagtttac atgaagagca agttagtgac cacagcagga caagatccca ggtgtctgag
     4321 ctcctacata aggttctttt tagtacacca catttatcta ggaagggacc ttgtaggtgg
     4381 tggctttaat ggcccttttg ggaggtattg gcttttatcc tctttctaga gcctctcata
     4441 tttgccagca agtagaaaat ttcaagatgc aagcctctgg tccctttgac tcctgctgga
     4501 gtcattgaca gcgcactaaa gcaaaggcag tgagtgcagt gaggaaggaa tctgagcaac
     4561 agacagtttt gaaccctaat gcttctgttt ctgtgcagat gcctgggcca cagcctccaa
     4621 ggctttcgcg gctcttattg agctctgctc ttggagctgt agcctatctg ttaatgattg
     4681 aaaagcaagc atatgggcag aacttggcca caggagtggg ttactaggtc ttcagatcca
     4741 tgaaagatta taaagacagt ttcagtctgg gttttgtgag ggaaaatact gcagtaagca
     4801 ctaaaaacat aagaacacta ttggaaggca gagaaagaca tggcaatccc aattgaagaa
     4861 gtgtctagaa acttggcact gtgattgcag tattacacgt gagtcatgga tgaaaactca
     4921 gctgggaaag gtgggggcac caattttgga ggccagagag cagttggtgg ggtagttctg
     4981 ctcctgcagt taactctctt taaagaccga aacaaaccat ctccctttaa aaatcagaat
     5041 gggctgtggt gaagatactc ttatgaaatg ggattggctt gcccatgatg cataggcact
     5101 tgctcagaac atgtgatggt gtgagtggta gctgtgggaa attagcagca accctgtcaa
     5161 gtaagtgaga gaactgttaa gtcttggaca gtgctaacac aagtgctaac cctgattaat
     5221 cacccactaa gtactgggta cttcaaatgc cctattcttc atccctataa caatcatgta
     5281 aggactcatc accttgtcca gatcaagagt acccagttgc attaagttac tggtctagta
     5341 cacagacatc atgaatacca gaaatgtgat ttgtatccag ccctttctgc ctttgcatcc
     5401 tgtgaaattt tcactgtgcc attctgaggt aggtatttaa atagaggcca catgattggc
     5461 agtggatgga cacttgattc ctgtgttcct aacaaatacc gaaaggaaat cagtggggtt
     5521 ggggggaggg aagtagtgca agtctacttc ataaggttag taaaatatag gataaactat
     5581 actccagagt tggattttct tttcactctg ggtttaccca tttggtaata tgttttaaaa
     5641 gatcacacaa cctcaccttg tgaagtgaag aatttgttat gattagtagc tgtgaattct
     5701 ttgccttagt catttctttg aaagacaaag agtttttcta ggtgttgctg tgctttctga
     5761 gtatgccctg gtttcatcca gaaggctggt gacatccaaa catgcagatt gaagaggaaa
     5821 gttctctgtg cttctgtgga attagatgcc ctgcggtcat atccctacaa tgtttttctg
     5881 cggtgaaagc aaaaaccagg tcatttaata aaagaaattt cactgggttt tttcatttca
     5941 agttgaaaat gaaaaggagg tggtatggag tatccagcca ccacggggca aaactaagca
     6001 ttcaatttaa atagtaaaac tctgtcatgt agggtacttc tgaaataatg ggctacaata
     6061 gagaaacatc tttccatgtt caagatgttc ctttattctg taagaactac aaaaagtaaa
     6121 ggaattagtt caacaacttg taagataatt gcttttgaga gaacatagat cagtgaagct
     6181 gttattaaag tagcagcaga caagtggcct tgacatatta caagcctaca aaagctttag
     6241 aaacaacagc aacagaaaat ccagacagaa gtaaagtaac ttgacaagat attttcaatg
     6301 aagaaaatcc ttcattttca tagggagatg cagtacttta tgtcaaggac actaatagag
     6361 gccttaaaag attgcctatg tgtaccgtgt gtatgaaacc agagaaaaga atgagatccc
     6421 tgcgcccatt catgtgcttc tccgttaagt gtgcagaatc tcttgtatga atgtcctccc
     6481 tctgcagtct catcatggaa gtagtctttc aacatatcag gaaggatggt ccacccacta
     6541 cctaccactt ctgctgcctt gtaagactct aaagagaacc cagcacaaaa ttcttttgga
     6601 cctaagagtc ttaatttagt ctccactttg caacatagcc ttgtcattct cttaggcctc
     6661 acagcatcag tacatgatag ctggcaattc tgtcttgtac ataaggaaat gtgaagccca
     6721 aggagctgaa aatgagttgt ccatgagcag aatctagaag ttgataaaaa aatccagggt
     6781 tgagaacttt atcaaacagt cacaaagcag tagtgaaaat gaatcccagg gccagagaga
     6841 tggctcagtg attaggagtt ctggttgctc ttccagttgt cctaagttca attcccagca
     6901 actacatggc tgctcacaaa catctataaa tgtagctcca taggatccaa tgcccccatc
     6961 tgcatgcaga caaaatgccc atatacaaca aattcataaa tacaccttta aaagaaaatg
     7021 aatccaaaat gtaactggag atgtttacaa acaggattaa tgaaaacata agtatcagca
     7081 ttacattacc tggggacttg ttagaaatgc tgattcttgg gatctaccct agacatctgg
     7141 aataagaatc tgtattttaa caagacccca caagagattt ctatgcacat taaaaatttg
     7201 agaaacaaaa aaaaaa
//
