LOCUS       NM_153520               2353 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus oligodendrocytic myelin paranodal and inner loop
            protein (Opalin), mRNA.
ACCESSION   NM_153520
VERSION     NM_153520.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Yu,Z., Yang,Z., Ren,G., Wang,Y., Luo,X., Zhu,F., Yu,S., Jia,L.,
            Chen,M., Worley,P.F. and Xiao,B.
  TITLE     GATOR2 complex-mediated amino acid signaling regulates brain
            myelination
  JOURNAL   Proc Natl Acad Sci U S A 119 (3) (2022)
   PUBMED   35022234
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Hardt,R., Jordans,S., Winter,D., Gieselmann,V., Wang-Eckhardt,L.
            and Eckhardt,M.
  TITLE     Decreased turnover of the CNS myelin protein Opalin in a mouse
            model of hereditary spastic paraplegia 35
  JOURNAL   Hum Mol Genet 29 (22), 3616-3630 (2021)
   PUBMED   33215680
  REMARK    GeneRIF: Decreased turnover of the CNS myelin protein Opalin in a
            mouse model of hereditary spastic paraplegia 35.
REFERENCE   3  (bases 1 to 2353)
  AUTHORS   de Faria,O. Jr., Dhaunchak,A.S., Kamen,Y., Roth,A.D., Kuhlmann,T.,
            Colman,D.R. and Kennedy,T.E.
  TITLE     TMEM10 Promotes Oligodendrocyte Differentiation and is Expressed by
            Oligodendrocytes in Human Remyelinating Multiple Sclerosis Plaques
  JOURNAL   Sci Rep 9 (1), 3606 (2019)
   PUBMED   30837646
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2353)
  AUTHORS   Yoshikawa,F., Sato,Y., Tohyama,K., Akagi,T., Furuse,T.,
            Sadakata,T., Tanaka,M., Shinoda,Y., Hashikawa,T., Itohara,S.,
            Sano,Y., Ghandour,M.S., Wakana,S. and Furuichi,T.
  TITLE     Mammalian-Specific Central Myelin Protein Opalin Is Redundant for
            Normal Myelination: Structural and Behavioral Assessments
  JOURNAL   PLoS One 11 (11), e0166732 (2016)
   PUBMED   27855200
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2353)
  AUTHORS   Xiao,J., Vemula,S.R., Xue,Y., Khan,M.M., Kuruvilla,K.P.,
            Marquez-Lona,E.M., Cobb,M.R. and LeDoux,M.S.
  TITLE     Motor phenotypes and molecular networks associated with germline
            deficiency of Ciz1
  JOURNAL   Exp Neurol 283 (Pt A), 110-120 (2016)
   PUBMED   27163549
REFERENCE   6  (bases 1 to 2353)
  AUTHORS   Yoshikawa,F., Sato,Y., Tohyama,K., Akagi,T., Hashikawa,T.,
            Nagakura-Takagi,Y., Sekine,Y., Morita,N., Baba,H., Suzuki,Y.,
            Sugano,S., Sato,A. and Furuichi,T.
  TITLE     Opalin, a transmembrane sialylglycoprotein located in the central
            nervous system myelin paranodal loop membrane
  JOURNAL   J Biol Chem 283 (30), 20830-20840 (2008)
   PUBMED   18490449
  REMARK    GeneRIF: highly sialylglycosylated Opalin has a role in an
            intermembranous function of the myelin paranodal loops in the
            central nervous system
REFERENCE   7  (bases 1 to 2353)
  AUTHORS   Kippert,A., Trajkovic,K., Fitzner,D., Opitz,L. and Simons,M.
  TITLE     Identification of Tmem10/Opalin as a novel marker for
            oligodendrocytes using gene expression profiling
  JOURNAL   BMC Neurosci 9, 40 (2008)
   PUBMED   18439243
  REMARK    GeneRIF: Together, our results demonstrate that Tmem10 is a novel
            marker for in vitro generated oligodendrocytes.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2353)
  AUTHORS   Aruga,J., Yoshikawa,F., Nozaki,Y., Sakaki,Y., Toyoda,A. and
            Furuichi,T.
  TITLE     An oligodendrocyte enhancer in a phylogenetically conserved intron
            region of the mammalian myelin gene Opalin
  JOURNAL   J Neurochem 102 (5), 1533-1547 (2007)
   PUBMED   17442045
REFERENCE   9  (bases 1 to 2353)
  AUTHORS   Montelius,A., Marmigere,F., Baudet,C., Aquino,J.B., Enerback,S. and
            Ernfors,P.
  TITLE     Emergence of the sensory nervous system as defined by Foxs1
            expression
  JOURNAL   Differentiation 75 (5), 404-417 (2007)
   PUBMED   17309606
REFERENCE   10 (bases 1 to 2353)
  AUTHORS   Nobile,C., Hinzmann,B., Scannapieco,P., Siebert,R., Zimbello,R.,
            Perez-Tur,J., Sarafidou,T., Moschonas,N.K., French,L., Deloukas,P.,
            Ciccodicola,A., Gesk,S., Poza,J.J., Lo Nigro,C., Seri,M.,
            Schlegelberger,B., Rosenthal,A., Valle,G., Lopez de Munain,A.,
            Tassinari,C.A. and Michelucci,R.
  TITLE     Identification and characterization of a novel human brain-specific
            gene, homologous to S. scrofa tmp83.5, in the chromosome 10q24
            critical region for temporal lobe epilepsy and spastic paraplegia
  JOURNAL   Gene 282 (1-2), 87-94 (2002)
   PUBMED   11814680
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121873.3.
            
            On Feb 7, 2019 this sequence version replaced NM_153520.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140031.1, BK000002.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN02415132 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AC121873.3         106155-106234       c
            81-116              AC121873.3         101317-101352       c
            117-152             AC121873.3         99039-99074         c
            153-275             AC121873.3         96778-96900         c
            276-332             AC121873.3         95620-95676         c
            333-2353            AC121873.3         91080-93100         c
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 34.36 cM"
     gene            1..2353
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /note="oligodendrocytic myelin paranodal and inner loop
                     protein"
                     /db_xref="GeneID:226115"
                     /db_xref="MGI:MGI:2657025"
     exon            1..80
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..509
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /note="transmembrane protein 10; transmembrane protein
                     TMP10"
                     /codon_start=1
                     /product="opalin"
                     /protein_id="NP_705740.1"
                     /db_xref="CCDS:CCDS37985.1"
                     /db_xref="GeneID:226115"
                     /db_xref="MGI:MGI:2657025"
                     /translation="MSFSLNFTLPSNTTSSPVVTSAKATDCGPSIGLAAGIPSLLATA
                     LLVALLFTLIQRRRTIDDEPVEETEIPCEISELYDNPKISENPRRSPTHEMNPRGSQE
                     GHIYVKTVSGSEEPLPDTYRPPEELERRRGLWWLVPSLSLE"
     exon            81..116
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /inference="alignment:Splign:2.1.0"
     exon            117..152
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /inference="alignment:Splign:2.1.0"
     exon            153..275
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /inference="alignment:Splign:2.1.0"
     exon            276..332
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /inference="alignment:Splign:2.1.0"
     exon            333..2353
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      872..877
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /note="hexamer: AATAAA"
     polyA_site      877
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /note="major polyA site"
     regulatory      1755..1760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /note="hexamer: AATAAA"
     polyA_site      1775
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
     regulatory      1812..1817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
                     /note="hexamer: TATAAA"
     polyA_site      1839
                     /gene="Opalin"
                     /gene_synonym="Tmem10; Tmp10"
ORIGIN      
        1 agctggtgcc ctacaaatgg gagacaaaga cctgcagtgc cagtgagaga gaagcctgcc
       61 aagggggcct gtcagaaatg agtttttcac tgaactttac actgccatcg aatacgacat
      121 cctctccagt tgtcacgagt gccaaagcca cagactgtgg tccctctatt ggactggcag
      181 ccggaatccc atccctgctg gccacagccc tgctggtggc tttgctgttt accttgatcc
      241 agcgaagaag aaccatcgat gatgagcccg tagaggagac tgagatacca tgtgaaattt
      301 cagaactcta tgacaatccc aaaatatctg agaatcctag gcggtcaccc acacatgaaa
      361 tgaacccacg gggcagccaa gaagggcaca tatatgtgaa gactgtctcg ggaagcgagg
      421 agcccctgcc ggacacctac cgtcctcctg aagaactaga aaggagaaga ggactttggt
      481 ggcttgtacc cagcctgagc ctagaatgat gtcaccgcat cagggagcag acccagagct
      541 ggatcagggc tgaaaacctg gaatctgtgc tcagggttca tgcttggaga ggcgaggagt
      601 cagctgcctc tcactcaaca tcaatttcat ttatagatcc aagacacatc tggctgcctt
      661 gcccctttag gggatggttg caggcaggtt cattcgatct cagaccttgc agggtaatca
      721 gcaaggcgac ccaggtgagt cgactatgtg tccagatgag ccccgtatgt cctgtttctg
      781 aatcgagaga agtctgcctt tgggaaacgg cttggctccc gagtgtcctg ccttctcctc
      841 tacctgctcc aaatgttatc atttaaaaaa taataaagct ttgagagttg gcttttttgg
      901 ggggtgcatt gaccaaggcc agcttggcac aagttaggac aggcataaaa cctaaccaaa
      961 gacatgtgac agaagccatt tcttcctttt gaatatctca ggataaagct cgcatggagc
     1021 cgtccatgcc ccgacacaag gaagtatgtg gctgatttgt gtcatacagc tgagaatgag
     1081 ctaggcactc cctagtcaga cgtgctgctg agcactttga gaagtcagat gagctcatct
     1141 accctccccg agcctgtttt gggttctgtg cttcctttgg ccactggaaa aattacagca
     1201 ggaataaagg aagcaatttc cacttagtag acactagaga tatcaatcct cttcacccag
     1261 ttcttcccac atcttcccat cctacttatc atccatccag agtcacaaag tcgacaagga
     1321 aatctaaacc ttctctgtgg cctgtaatct gaatttttga catttaaaaa taactctagt
     1381 ggtcactggc agtctcctca cttcgactct gcacaccatt tttttcccct tgggtgtcaa
     1441 ccaagttcca aatcacaagt acagagatct gtgttacaca ttacatatta acctttagga
     1501 tgctcgagtc ctggatggtt aatcttcact gtcaactcaa tgggattgag aatcaccaag
     1561 gagacacacc tccagaaaga tttaactgag taagaagatc cacctgaatg tggcccagca
     1621 tcattccata gcctggggtt ctgggaattc aagttgagca tgcagaactc atcactatcc
     1681 ttcctgacga cagataaagt gtttggccag ctaccccaca ctcctgctgc cacacctttc
     1741 ttggactgaa ccaaaataaa tacctccacc cttaagttga ctttatcata acactgagag
     1801 aagtaagcta atataaaact gatattcatg ttttaatgac ttttctttgt aatgagccat
     1861 ttgctttgaa gtaccataaa gaaaagatgt ttgtgaggga gaggcatcaa gaggaccaaa
     1921 gatgaacaca ggagcaaagg gatgggttgg gatagctaca cctgctatgc aagggacatc
     1981 tagggacaac ttcaccagtt cgggcctgat ctttgccttc acttccccag acagtttcct
     2041 tgtgaccttc tagtgcacag ccactgcctt gaaatacacc atgcttcaca gtgggagagc
     2101 tgaggcctca gggcatctct agaaacatcc atggtctcct tgagccacct tgatatccca
     2161 gagaaccaac tcttcttgca tggggcctct ggttgtttag aaatagttgg atttcttgag
     2221 ctatagggaa tggttctgta aacccaatga ccatcacatg ttaacagctg cctttcaaag
     2281 cccctctgaa attcaatttt gggttatttc atgggctcag gtcttagata aacacatttg
     2341 aacatcttca atc
//
