LOCUS       NM_001403170            6061 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus OPA1, mitochondrial dynamin like GTPase (Opa1),
            transcript variant 3, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001403170 XM_006522654
VERSION     NM_001403170.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Liang,F.G., Zandkarimi,F., Lee,J., Axelrod,J.L., Pekson,R.,
            Yoon,Y., Stockwell,B.R. and Kitsis,R.N.
  TITLE     OPA1 promotes ferroptosis by augmenting mitochondrial ROS and
            suppressing an integrated stress response
  JOURNAL   Mol Cell 84 (16), 3098-3114 (2024)
   PUBMED   39142278
  REMARK    GeneRIF: OPA1 promotes ferroptosis by augmenting mitochondrial ROS
            and suppressing an integrated stress response.
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Ahola,S., Pazurek,L.A., Mayer,F., Lampe,P., Hermans,S., Becker,L.,
            Amarie,O.V., Fuchs,H., Gailus-Durner,V., de Angelis,M.H.,
            Riedel,D., Nolte,H. and Langer,T.
  TITLE     Opa1 processing is dispensable in mouse development but is
            protective in mitochondrial cardiomyopathy
  JOURNAL   Sci Adv 10 (31), eadp0443 (2024)
   PUBMED   39093974
  REMARK    GeneRIF: Opa1 processing is dispensable in mouse development but is
            protective in mitochondrial cardiomyopathy.
REFERENCE   3  (bases 1 to 6061)
  AUTHORS   Castellaneta,A., Losito,I., Porcelli,V., Barile,S., Maresca,A., Del
            Dotto,V., Losacco,V., Guadalupi,L.S., Calvano,C.D., Chan,D.C.,
            Carelli,V., Palmieri,L. and Cataldi,T.R.I.
  TITLE     Lipidomics reveals the reshaping of the mitochondrial phospholipid
            profile in cells lacking OPA1 and mitofusins
  JOURNAL   J Lipid Res 65 (6), 100563 (2024)
   PUBMED   38763493
  REMARK    GeneRIF: Lipidomics reveals the reshaping of the mitochondrial
            phospholipid profile in cells lacking OPA1 and mitofusins.
REFERENCE   4  (bases 1 to 6061)
  AUTHORS   Luo,J., Hu,S., Liu,J., Shi,L., Luo,L., Li,W., Cai,Y., Tang,J.,
            Liu,S., Fu,M., Dong,R., Yang,Y., Tu,L. and Xu,X.
  TITLE     Cardiac-specific PFKFB3 overexpression prevents diabetic
            cardiomyopathy via enhancing OPA1 stabilization mediated by
            K6-linked ubiquitination
  JOURNAL   Cell Mol Life Sci 81 (1), 228 (2024)
   PUBMED   38777955
  REMARK    GeneRIF: Cardiac-specific PFKFB3 overexpression prevents diabetic
            cardiomyopathy via enhancing OPA1 stabilization mediated by
            K6-linked ubiquitination.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6061)
  AUTHORS   Akepati,V.R., Muller,E.C., Otto,A., Strauss,H.M., Portwich,M. and
            Alexander,C.
  TITLE     Characterization of OPA1 isoforms isolated from mouse tissues
  JOURNAL   J Neurochem 106 (1), 372-383 (2008)
   PUBMED   18419770
  REMARK    GeneRIF: Manipulation of OPA1 isoform 1 levels in induces changes
            in the mitochondrial network in the cell; mutations of isoform 1
            could lead to devastating effects on retinal ganglion cells.
REFERENCE   6  (bases 1 to 6061)
  AUTHORS   Alavi,M.V., Bette,S., Schimpf,S., Schuettauf,F., Schraermeyer,U.,
            Wehrl,H.F., Ruttiger,L., Beck,S.C., Tonagel,F., Pichler,B.J.,
            Knipper,M., Peters,T., Laufs,J. and Wissinger,B.
  TITLE     A splice site mutation in the murine Opa1 gene features pathology
            of autosomal dominant optic atrophy
  JOURNAL   Brain 130 (Pt 4), 1029-1042 (2007)
   PUBMED   17314202
REFERENCE   7  (bases 1 to 6061)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 6061)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   9  (bases 1 to 6061)
  AUTHORS   Delettre,C., Lenaers,G., Belenguer,P. and Hamel,C.P.
  TITLE     Gene structure and chromosomal localization of mouse Opa1 : its
            exclusion from the Bst locus
  JOURNAL   BMC Genet 4, 8 (2003)
   PUBMED   12735796
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 6061)
  AUTHORS   Misaka,T., Miyashita,T. and Kubo,Y.
  TITLE     Primary structure of a dynamin-related mouse mitochondrial GTPase
            and its distribution in brain, subcellular localization, and effect
            on mitochondrial morphology
  JOURNAL   J Biol Chem 277 (18), 15834-15842 (2002)
   PUBMED   11847212
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154438.2.
            
            On Mar 28, 2022 this sequence version replaced XM_006522654.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.18331.1,
                                           SRR10223969.16708.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AC154438.2         205090-205290       c
            202-520             AC154438.2         198325-198643       c
            521-617             AC154438.2         197595-197691       c
            618-725             AC154438.2         196302-196409       c
            726-779             AC154438.2         195785-195838       c
            780-847             AC154438.2         194913-194980       c
            848-958             AC154438.2         186965-187075       c
            959-1012            AC154438.2         182396-182449       c
            1013-1117           AC154438.2         179045-179149       c
            1118-1204           AC154438.2         176892-176978       c
            1205-1318           AC154438.2         176195-176308       c
            1319-1399           AC154438.2         173913-173993       c
            1400-1474           AC154438.2         173733-173807       c
            1475-1546           AC154438.2         173193-173264       c
            1547-1646           AC154438.2         173011-173110       c
            1647-1777           AC154438.2         172624-172754       c
            1778-1850           AC154438.2         170625-170697       c
            1851-1923           AC154438.2         170463-170535       c
            1924-2039           AC154438.2         169497-169612       c
            2040-2104           AC154438.2         168690-168754       c
            2105-2181           AC154438.2         167970-168046       c
            2182-2347           AC154438.2         166374-166539       c
            2348-2500           AC154438.2         164128-164280       c
            2501-2609           AC154438.2         163782-163890       c
            2610-2689           AC154438.2         163117-163196       c
            2690-2830           AC154438.2         159057-159197       c
            2831-2947           AC154438.2         155848-155964       c
            2948-3041           AC154438.2         154942-155035       c
            3042-3152           AC154438.2         154467-154577       c
            3153-3227           AC154438.2         135650-135724       c
            3228-6061           AC154438.2         130074-132907       c
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 20.65 cM"
     gene            1..6061
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /note="OPA1, mitochondrial dynamin like GTPase"
                     /db_xref="GeneID:74143"
                     /db_xref="MGI:MGI:1921393"
     exon            1..201
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..3217
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /EC_number="3.6.5.5"
                     /note="isoform 3 is encoded by transcript variant 3;
                     dynamin-like 120 kDa protein, mitochondrial; largeG; large
                     GTP-binding protein; optic atrophy protein 1 homolog;
                     optic atrophy 1 homolog; dynamin-like GTPase OPA1,
                     mitochondrial"
                     /codon_start=1
                     /product="dynamin-like GTPase OPA1, mitochondrial isoform
                     3"
                     /protein_id="NP_001390099.1"
                     /db_xref="GeneID:74143"
                     /db_xref="MGI:MGI:1921393"
                     /translation="MWRAGRAAVACEVCQSLVKHSSGIQRNVPLQKLHLVSRSIYRSH
                     HPALKLQRPQLRTPFQQFSSLTHLSLHKLKLSPIKYGYQPRRNFWPARLAARLLKLRY
                     IILGSAVGGGYTAKKTFDEWKDMIPDLSDYKWIVPDFIWEIDEYIDLEKIRKALPSSE
                     DLASLAPDLDKITESLSLLKDFFTAGPKLVSEVLEVSEALLLLGSPGETAFRATDHGS
                     ESDKHYRKGLLGELILLQQQIQEHEEEARRAAGQYSTSYAQQKRKVSDKEKIDQLQEE
                     LLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYD
                     ASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHH
                     VALFKDSSREFDLTKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMV
                     LVDLPGVINTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVS
                     QMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSES
                     IEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESVEQQADSFKA
                     TRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPKHWEEILQQSLWER
                     VSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFM
                     TEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDA
                     AIYFMEEALQGRLKDTENAIENMIGPDWKKRWMYWKNRTQEQCVHNETKNELEKMLKV
                     NDEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRG
                     FYYYQRHFIDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDF
                     AEDGEKKVKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK"
     exon            202..520
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            521..617
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            618..725
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            726..779
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            780..847
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            848..958
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1012
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1117
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1204
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1318
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1399
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1474
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1546
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1646
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1777
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1850
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1923
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2039
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2104
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2181
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2347
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2500
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2609
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2689
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2690..2830
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2831..2947
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3041
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3152
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            3153..3227
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     exon            3228..6061
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3330..3335
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /note="hexamer: AATAAA"
     polyA_site      3353
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /note="major polyA site"
     regulatory      6042..6047
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /note="hexamer: ATTAAA"
     polyA_site      6061
                     /gene="Opa1"
                     /gene_synonym="1200011N24Rik; lilr3; mKIAA0567"
                     /note="major polyA site"
ORIGIN      
        1 ggctcttgcg gaagtccatg cgccattggg agggactcgg tggctgctct atgcccttgt
       61 tgctgagggc ctcttcctgg gtcattgtcg gagcaggaat cggactgggg ccgacacggg
      121 gttccccccg tggccgtctc agcgcctgtg tgtggtctgc tctggcggga tgtggcgagc
      181 aggtcgggcg gccgtggcct gtgaagtctg ccaatcctta gtgaaacaca gttctggaat
      241 acaaagaaac gtaccgctcc aaaaactcca tctggtttca cgaagtattt atcgttcaca
      301 tcatcctgcc ctcaagcttc aaagacccca actaaggaca ccatttcagc agttctcttc
      361 tctaactcac ctttcattac ataaattgaa actttctcca attaaatatg gctaccagcc
      421 ccgcaggaac ttttggccag caaggttagc tgcaagactc ttaaaacttc gatatatcat
      481 actgggatct gctgttggag gtggctatac agccaaaaaa accttcgatg aatggaaaga
      541 tatgatacca gaccttagtg actataagtg gattgtgcct gactttatat gggaaattga
      601 tgagtatatt gatttggaga aaattagaaa agccctgccc agctcagaag accttgccag
      661 tttagctccc gacctggaca agattactga gagcctcagc ttgttgaagg acttcttcac
      721 tgcaggtccc aaattggtta gtgaagtcct agaagtttct gaggcccttc tcttgttagg
      781 ttcacctgga gaaacagcat ttcgagcaac agatcatgga tctgaaagtg acaagcatta
      841 caggaagggt ctgcttggtg agctcattct cttacaacag caaattcaag agcacgaaga
      901 ggaagcacgc agagccgctg gccagtatag cacgagctat gcccaacaga agcgcaaggt
      961 gtcagacaaa gaaaagattg accaacttca agaagaactt ctgcatactc agttaaagta
     1021 tcagaggatc ttggagcgcc tggaaaagga gaacaaagag ctgcggaagc tggtgctgca
     1081 gaaggacgac aaaggcatcc accacaggaa gctcaagaaa tctttgattg atatgtattc
     1141 tgaagttctt gatgttcttt ctgattatga tgccagttac aatacacaag atcacctacc
     1201 acgggttgtt gtggttggag atcagagtgc tgggaaaacc agtgtgctgg aaatgattgc
     1261 tcaggcccgg atcttcccga gagggtccgg cgagatgatg acacgctctc cagtgaaggt
     1321 gactctcagt gaaggccctc accatgtggc cttgtttaaa gatagctctc gggaatttga
     1381 tctcaccaag gaggaagatc ttgcagcatt aagacatgaa atcgaactcc gaatgaggaa
     1441 aaatgtgaaa gaaggttgta ctgttagccc cgagaccata tctctaaatg tcaaaggccc
     1501 tgggctgcag aggatggtgc tcgtggactt gcctggtgtc atcaacaccg tgacatcagg
     1561 catggctccc gacacaaagg aaactatttt cagtatcagc aaagcttaca tgcagaatcc
     1621 taacgccatc atcctgtgca tccaagacgg atccgtagat gctgagcgca gtattgttac
     1681 agacttggtc agtcaaatgg atcctcatgg aagaagaacc atatttgttt tgaccaaagt
     1741 agacctggca gaaaaaaatg tagccagtcc aagcaggata caacagataa ttgaaggcaa
     1801 gctcttccca atgaaagctc tgggttattt tgctgtcgta acaggaaaag gaaacagctc
     1861 tgaaagtatt gaagctataa gagagtatga agaggaattt tttcagaatt caaaactgct
     1921 aaagacaagc atgctaaagg cacaccaggt caccacgaga aatctcagcc ttgctgtgtc
     1981 agactgcttt tggaaaatgg ttcgagagtc agttgaacaa caggctgata gttttaaagc
     2041 cacgcgcttt aacctagaga cggaatggaa gaataactac ccgcgcctgc gagagctcga
     2101 caggaatgaa ctctttgaaa aagctaaaaa tgagatcctc gatgaggtca tcagtctgag
     2161 ccaggtcacg ccaaagcact gggaggaaat cctgcagcaa tccctgtggg aacgagtgtc
     2221 aacacatgtg attgagaaca tctaccttcc agctgcccag accatgaatt cgggaacatt
     2281 taacaccaca gtagacatca agcttaaaca gtggactgac aagcagcttc ctaataaagc
     2341 agtcgaggtt gcctgggaga ctctacaaga ggaattttcc cgcttcatga cagaacccaa
     2401 aggaaaggaa cacgacgaca tatttgacaa acttaaggag gctgtgaagg aggagagtat
     2461 caagcggcac aagtggaacg actttgccga ggatagcttg agggttattc agcacaatgc
     2521 tttggaagac cggtccatat cagataagca acagtgggat gcagccattt acttcatgga
     2581 agaggcgctt caaggtcgtc tcaaggatac tgaaaatgct attgaaaaca tgattgggcc
     2641 agactggaaa aagaggtgga tgtactggaa gaatcggacc caagagcagt gtgttcacaa
     2701 cgaaaccaag aacgagttgg agaagatgct gaaggttaat gatgagcacc cagcttacct
     2761 ggcaagtgat gagattacca cagtccggaa gaacctggag tctcgaggag tggaagtcga
     2821 tccaagcttg attaaggata cttggcatca agtttataga agacatttct taaaaacagc
     2881 tctaaatcat tgtaaccttt gtcgcagagg tttttattac taccagaggc attttataga
     2941 ttctgagctg gaatgcaatg acgtggtcct gttttggcga atacagcgca tgctcgctat
     3001 cactgccaat acattaaggc agcagcttac aaacactgaa gttaggcgac tagagaaaaa
     3061 cgttaaagag gtattagaag attttgcaga agacggtgag aagaaggtta aattgctcac
     3121 tggcaaacga gttcagctgg cagaagatct caagaaagtt agagaaattc aagaaaagct
     3181 tgatgctttc attgaagctc ttcaccagga gaagtagact gtgtcaaaat cctgctcata
     3241 atgtaatcac ctctgcgttt atttgaagaa tgaaaacttg agtcttttgt cctgcctcct
     3301 ttcctcccgt tctacccttt tccgtgcaaa ataaagtcac agggtaaaag tcatgtacgt
     3361 gtgttccagg cacttaacct gcagctgccg tttgagcaga agagccctgg acgtggcatc
     3421 cacggtctgt gagctcgtgc tgacggcaga cattgggaaa acatcactgg tgtggccgtg
     3481 aggcttagtc ctcagagatt aagaagttgc tctctgagtc gtgtttgtgg tggcactgat
     3541 gtgattcact atggctggta tcacacgtac agtggtagct gctctggaat aaaacttaca
     3601 gtaatttttt aaaaggcatc aattgtatat gacaagatga cagttactgc taaaccatca
     3661 tgtctacagt gttctgtatt caggaacgct ttgactgtaa acactcagtt tatgttcacc
     3721 acatgaaaga aagctggttt ataaacagat gcttcagctg ggttaattta tgttgggcag
     3781 ttcagtgtat attcagtgaa agaaacatcc accctccatt gccagcttag aaactcgcct
     3841 tggaagccaa ccgcagcagc gtgcagtcca tcgctgcttt tgcgccctgt atccgtgtgc
     3901 acttagttca gtttgaatat tgtgtgctct cagtcaggct ttctggaaca gtaggtctct
     3961 cctggtgaca cagccattga attatgaccc acgctggaga agactaaaga ccaccagttg
     4021 acgaatttca tttagagtgc tatcaaatgt ctgaactagg cgtcagggaa aggtttcaca
     4081 atagccctca aacactattt taaagaaaaa ttattctaac aagggaaaaa tgttgaccca
     4141 ctcccactta aagctgaaaa ttctgtttaa ttcattgcag ttatagtaat aaccttcata
     4201 atattttcat gcgctttata taaaattacg ttggttaaaa aagcttaagg caaacagtac
     4261 ttcatattta cttcaaatgt ctttttcatc tgccaccttt tttattaagg ctttactgtt
     4321 ctggtaaaaa cacccagggt tgcagttctt atttacagtt aattttataa ccagtttcct
     4381 tggaaatgtt tttgtttatg attccctcga atctgacagt atggaagggg cagtgtttca
     4441 gaaaccatga attcaataag tactctattt aaattatcaa taagaagcct gggtgcaatt
     4501 ctcagagcta cgacgaacag tgcccatcct tccctaggac aaatgtgcag gttgtaggtg
     4561 tgggacagta gcgaacagaa gctctagaga gccctgggct gattcccaca gccatgagga
     4621 tatgctcgtc ctaagccagt ggtcctcaac ctgtgggcca agagcacttt gggggctgca
     4681 tatcagctac ttacattatg atttgtaaca gggacagaat tacagttatg gaagagcaac
     4741 aaaatgatgt catggatggg ggtcaccaca gcacgaggac tgtatcaaag ggtcccagca
     4801 ttaggaaggt tgagaactgc tgccctaagc cctgctagcc tcactagggt ggataggctg
     4861 aggccagcaa gagaaatctg caactttctc ccaagtaaaa gtcttagatt ggattcatat
     4921 ctctttatac ttttttttta agttgaagag aacttttatt tctttgcagg gatctaaatt
     4981 tgtatcttca acttaaacgt atattactaa aaaccctttc actcttgacc cctgtgggtt
     5041 atttgtaacc tggtttcctg ctaactgccc ccatcccctg aaagctatgt gtgtacgaaa
     5101 cgcagaagct acaggaaggg taaagttgag tcccagcagt agaccctgtg tatcatgtct
     5161 gtagttcagg tgctcaaata tacacaagcc aaacttggac tggacaagaa gccatttgca
     5221 aaaatggagt ggataggttt ttctttttta gacaaaattt tatttggaga actaagggat
     5281 ttttaaatac atcataagca aaatgacttg gtcagaatgt cagttatgaa cccctagtct
     5341 gtgtgcagca tgaaccacag gaagcaagct tgctgcttca gccacacacc aagcaatctt
     5401 cagttagtta caggtgcagc agtctgggcc ttcctcttcg tctctcctca tcgggccccg
     5461 ttttccaggc tgtagtcagt ggactcccac agattcctca gtgaccaaat ggaaagggtt
     5521 ggtgtggaag caagtgagac ttcctaactg tacagcgtgg gatgtcacat gcaggattca
     5581 ggtggcagaa gcaggacagt cacaagtcaa aggcaagcct gagctacata gggacacccc
     5641 tgtctcaaaa acaaaaataa caaaattaag actttaggct cagaaagcaa atttcttatg
     5701 tagttaattt tgtatttatt tatttcagat gtatttatct gataaaagaa actttttttc
     5761 tctctccaat aaatgagatc acggtactgg tgtgtctcca tcctctacgc tggtgtatgg
     5821 tagattgtct tatactgtag caactggaaa tgtgcttgaa aatatgctta ccttctaaac
     5881 gatcatggct atttgatatc taaatattta aaacttaacc atgtcgtcac tgtgcactgt
     5941 gaatggactt tgtattattt ttaaacctta cactcgatgt aaatgttact acaacttata
     6001 tttgcctgtg cttgacctaa ggtcatttgt gtagatgggt aattaaagga tactgaagtg
     6061 a
//
