LOCUS       NM_010956               6630 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus oxoglutarate (alpha-ketoglutarate) dehydrogenase
            (lipoamide) (Ogdh), transcript variant 4, mRNA.
ACCESSION   NM_010956 XM_001475646 XM_001478677
VERSION     NM_010956.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Ayyangar,U., Karkhanis,A., Tay,H., Afandi,A.F.B., Bhattacharjee,O.,
            Ks,L., Lee,S.H., Chan,J. and Raghavan,S.
  TITLE     Metabolic rewiring of macrophages by epidermal-derived lactate
            promotes sterile inflammation in the murine skin
  JOURNAL   EMBO J 43 (7), 1113-1134 (2024)
   PUBMED   38418556
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6630)
  AUTHORS   Zhao,K., Huang,X., Zhao,W., Lu,B. and Yang,Z.
  TITLE     LONP1-mediated mitochondrial quality control safeguards metabolic
            shifts in heart development
  JOURNAL   Development 149 (6) (2022)
   PUBMED   35237793
REFERENCE   4  (bases 1 to 6630)
  AUTHORS   Andrade,J., Shi,C., Costa,A.S.H., Choi,J., Kim,J.,
            Doddaballapur,A., Sugino,T., Ong,Y.T., Castro,M., Zimmermann,B.,
            Kaulich,M., Guenther,S., Wilhelm,K., Kubota,Y., Braun,T., Koh,G.Y.,
            Grosso,A.R., Frezza,C. and Potente,M.
  TITLE     Control of endothelial quiescence by FOXO-regulated metabolites
  JOURNAL   Nat Cell Biol 23 (4), 413-423 (2021)
   PUBMED   33795871
REFERENCE   5  (bases 1 to 6630)
  AUTHORS   Wagner,M., Bertero,E., Nickel,A., Kohlhaas,M., Gibson,G.E.,
            Heggermont,W., Heymans,S. and Maack,C.
  TITLE     Selective NADH communication from alpha-ketoglutarate dehydrogenase
            to mitochondrial transhydrogenase prevents reactive oxygen species
            formation under reducing conditions in the heart
  JOURNAL   Basic Res Cardiol 115 (5), 53 (2020)
   PUBMED   32748289
  REMARK    GeneRIF: Selective NADH communication from alpha-ketoglutarate
            dehydrogenase to mitochondrial transhydrogenase prevents reactive
            oxygen species formation under reducing conditions in the heart.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6630)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 6630)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   8  (bases 1 to 6630)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 6630)
  AUTHORS   Udaka,K., Tsomides,T.J., Walden,P., Fukusen,N. and Eisen,H.N.
  TITLE     A ubiquitous protein is the source of naturally occurring peptides
            that are recognized by a CD8+ T-cell clone
  JOURNAL   Proc Natl Acad Sci U S A 90 (23), 11272-11276 (1993)
   PUBMED   8248240
REFERENCE   10 (bases 1 to 6630)
  AUTHORS   Udaka,K., Tsomides,T.J. and Eisen,H.N.
  TITLE     A naturally occurring peptide recognized by alloreactive CD8+
            cytotoxic T lymphocytes in association with a class I MHC protein
  JOURNAL   Cell 69 (6), 989-998 (1992)
   PUBMED   1606619
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147289.1 and AL607152.21.
            
            On Nov 9, 2011 this sequence version replaced NM_010956.3.
            
            Transcript Variant: This variant (4) differs in a region of the
            coding sequence, compared to variant 1. The resulting isoform (3)
            has a shorter and different internal segment, compared to isoform
            1. Variants 3 and 4 encode the same isoform 3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147289.1, AK150009.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5439              AK147289.1         2-5440
            5440-6217           AL607152.21        165134-165911
            6218-6630           AK147289.1         6219-6631
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.94 cM"
     gene            1..6630
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /note="oxoglutarate (alpha-ketoglutarate) dehydrogenase
                     (lipoamide)"
                     /db_xref="GeneID:18293"
                     /db_xref="MGI:MGI:1098267"
     exon            1..97
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            98..345
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..3195
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /EC_number="1.2.4.2"
                     /note="isoform 3 precursor is encoded by transcript
                     variant 4; 2-oxoglutarate dehydrogenase, mitochondrial;
                     OGDC-E1; alpha-KGDH-E1; thiamine diphosphate
                     (ThDP)-dependent 2-oxoglutarate dehydrogenase;
                     2-oxoglutarate dehydrogenase complex component E1;
                     alpha-ketoglutarate dehydrogenase; oxoglutarate
                     dehydrogenase (lipoamide)"
                     /codon_start=1
                     /product="2-oxoglutarate dehydrogenase complex component
                     E1 isoform 3 precursor"
                     /protein_id="NP_035086.2"
                     /db_xref="CCDS:CCDS36106.1"
                     /db_xref="GeneID:18293"
                     /db_xref="MGI:MGI:1098267"
                     /translation="MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPV
                     AAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRS
                     SLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSS
                     VPADIISSTDKLGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRLEMAYCQHI
                     GVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEK
                     RFGLEGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFD
                     SKLEAADEGSGDMKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQ
                     FYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTT
                     DPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRNTFHKDVVVDLVCY
                     RRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKIC
                     EEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDVLFHIGKVASSV
                     PVENFTIHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGT
                     FSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNAL
                     VLWEAQFGDFNNMAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERF
                     LQMCNDDPDVLPDLQEENFDINQLYDCNWIVVNCSTPGNFFHVLRRQILLPFRKPLIV
                     FTPKSLLRHPEARTSFDEMLPGTHFQRVIPENGPAAQDPHKVKRLLFCTGKVYYDLTR
                     ERKARNMEEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPR
                     LRTTIDRAKPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS"
     exon            346..537
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            538..640
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            641..756
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            757..911
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1058
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1149
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1329
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1458
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1638
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1791
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1894
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2023
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..2174
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2302
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2481
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2553
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2554..2682
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2755
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2756..2919
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..3074
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     exon            3075..6615
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4141..4146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /note="hexamer: CATAAA"
     polyA_site      4163
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /note="major polyA site"
     regulatory      4983..4988
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /note="hexamer: AATAAA"
     polyA_site      5003
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
     regulatory      6593..6598
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
                     /note="hexamer: AATAAA"
     polyA_site      6615
                     /gene="Ogdh"
                     /gene_synonym="2210403E04Rik; 2210412K19Rik; d1401; E1o;
                     mKIAA4192; OGDH-E1"
ORIGIN      
        1 gggctacgcg ttgacgccac acgccggggc ggggctgaga gtttggagcc cggagtggtg
       61 tcggcgcttc actcgggtgg agctgaacgg gagacaggta cttgtggaag gcttcaggac
      121 aaaatgtttc atttaaggac ttgtgctgct aagttaaggc cattgacagc ctcccagact
      181 gttaagacat tttcacaaaa caaaccagca gcaattagga cgtttcaaca gattcggtgc
      241 tattctgcac ctgtagctgc tgaaccattt cttagtggga ctagttcgaa ctatgtggag
      301 gaaatgtact gtgcctggtt ggagaatccc aaaagtgtac ataagtcatg ggacattttt
      361 ttccgaaaca ccaatgctgg agccccaccg ggcactgcct accagagccc cctttccctg
      421 agtcgaagct ccctggctac catggcccat gcacagtccc tggtggaagc acaacctaac
      481 gtcgacaaac tcgtggagga ccacttggcg gtgcagtctc tcatcagggc atatcagata
      541 cgagggcacc atgtagcaca gctggacccc ctggggattt tggatgctga tctggactcc
      601 tccgtgcccg ctgacattat ctcatccaca gacaaacttg ggttctatgg cctacacgag
      661 tctgaccttg acaaggtctt ccacttaccc accaccactt tcatcggggg acaggagcca
      721 gcacttcctc ttcgggagat catccgtcgg ctggagatgg cctactgcca gcacattggt
      781 gtggagttca tgttcattaa tgatttggaa caatgccagt ggatccgaca gaagtttgag
      841 acccctggaa tcatgcagtt caccaatgag gagaagcgga ccttgctggc caggcttgta
      901 cgatccacca ggtttgagga gttcctacag cgaaagtggt cctcggagaa gcgttttggt
      961 ctggaaggct gtgaggtgct gatccctgcc ctcaagacaa tcattgatat gtcaagtgca
     1021 aatggagtgg actatgtgat catggggatg ccacacagag gacggctgaa cgtgcttgca
     1081 aatgtcatca ggaaggagct ggagcaaata ttctgtcagt ttgactcaaa gctggaggca
     1141 gctgatgagg gttctgggga catgaagtac cacctgggca tgtatcaccg caggatcaac
     1201 cgtgtgaccg acagaaacat cactttgtcc ttggtggcta acccttccca tctagaggct
     1261 gctgaccctg tcgtgatggg aaagaccaaa gctgaacagt tctactgtgg agacactgaa
     1321 gggaaaaagg tgatgtctat cctgctgcat ggggatgctg cttttgctgg ccagggcatc
     1381 gtatatgaga ccttccatct cagcgacttg ccgtcctaca caacccatgg cactgttcat
     1441 gtggttgtca acaaccagat tggcttcacc acagaccctc ggatggcccg ctcctctccc
     1501 taccccactg atgtggcccg agtggtgaat gcccccattt tccatgtcaa ctcagatgac
     1561 cctgaagctg tcatgtatgt atgcaaggtg gcagctgagt ggagaaacac cttccacaag
     1621 gatgttgtag ttgatctggt gtgttatcga cgaaatggcc acaatgagat ggacgaacct
     1681 atgtttacac agccactcat gtacaagcag atccgcaagc agaagcctgt actgcagaag
     1741 tatgcagaat tgctagtctc ccagggtgtc gtcaatcagc ctgagtacga ggaggaaatc
     1801 tccaagtatg ataagatctg tgaggaagca tttaccagat ccaaagatga gaagatcttg
     1861 cacatcaagc actggctgga ttccccctgg cctggctttt tcaccctgga tggacagccc
     1921 aggagcatga cctgcccctc cactggcctg gaggaggatg tcttgttcca cattggaaag
     1981 gtggccagct ctgtacctgt ggagaacttt actatccatg gagggctgag ccggatcttg
     2041 aagacccgca gagagcttgt gacgaaccgg actgtggact gggccctggc agagtacatg
     2101 gcatttggct cactgctgaa ggaaggcatc catgtgcggc tgagtggcca ggatgtggag
     2161 cggggcacct tcagccatcg ccaccatgtg ctccatgatc agaatgttga caaaagaacc
     2221 tgcatcccca tgaaccacct ttggccaaat caggcccctt acactgtatg caacagctcg
     2281 ctgtctgagt acggtgtcct gggctttgag ctgggctttg ccatggctag ccctaatgct
     2341 ctggttctct gggaggccca gtttggtgac ttcaacaaca tggcacagtg catcattgac
     2401 cagttcatct gcccaggaca ggcaaagtgg gtgcggcaga atggcattgt gctcctgctg
     2461 cctcatggca tggaaggcat gggtcccgag cattcctctg cccgcccaga gcggtttctg
     2521 cagatgtgca atgatgaccc agatgtcctg cctgacctgc aggaagaaaa ctttgacatc
     2581 aatcagctct atgactgcaa ctggattgtt gtcaactgtt ccacccctgg caacttcttc
     2641 catgtgctgc gacgacagat cttgctgccc ttccggaagc cgttaatcgt cttcactccc
     2701 aaatccctcc tgcgccaccc tgaggcaaga actagctttg acgagatgct gccaggaacg
     2761 cacttccagc gtgtgatccc agaaaatgga cctgcagctc aggacccaca caaagtcaag
     2821 agacttctct tctgcactgg gaaggtgtac tatgacctca cccgagagcg caaagccagg
     2881 aacatggagg aggaggtggc tattacaagg attgagcagc tatcaccatt cccctttgac
     2941 ctcctgctga aagaggcaca gaagtatccc aatgctgagc tggcctggtg ccaggaagag
     3001 cacaagaacc aaggctacta tgactatgtc aagccaagac ttcgtaccac cattgaccgt
     3061 gctaagcctg tctggtatgc tggccgagac ccggcagctg ctccagccac tggcaacaag
     3121 aaaacacacc tgacagagct gcagcgcttt ctggacacag cctttgacct ggacgcattc
     3181 aagaaattct cttagatgct cctggagttg atgaggccat ggcccccatg tccatgacgc
     3241 tctttgcttc tcaactaaag aatagtgcct cagcactgtc cacacgtccc ttcgctgtgc
     3301 cacaccaccc ctgttctcat aggaattaag ttgtccactg cagtgctcag ctgctccccg
     3361 gtcacatgct gcccagcctg tgccgacttc tctcaggctg cacaccgttc atggagaccg
     3421 gaaggagcag aataaggaaa gggcccctct caggacatcc tagagaagga aggcagctct
     3481 ggccccaccc atgcccccag tgcaatcctc cagggtagga acagaaccct atgtggcttc
     3541 ccagggtact agcactcagc cctcgtcacc catcaagtcg cagattcaag gccaggagta
     3601 gtttcatctt gctagggcca agctgagagc tcatggagga actatagctg ccaggatttg
     3661 ggagtcatca ggatgttgtg tgaatagaga ttgtcatggg gtatttagag gactttagca
     3721 gtgatgttag tctagccctg ctacccttct tgggtttggg ctgtatgtgg gaaacttacc
     3781 ccagctacca cgcctggaga gcttggctct gagtacggcc cagaagctcc attggctccc
     3841 aacgccaggc actgctgcct cttggtcctg ctgcctctgc tctcctgacc cctccccagt
     3901 cacttcattt tctctgttgt tcccttgaac acacagaagc tgttgacgaa ttcttttttt
     3961 tgctgtgcca aggcaggtca aaagcagatc agtggataag agcaagttgt cccaaggagc
     4021 cagctgtcct tcctccctct tttgacctcc actgggacac acctgattta tttattttgg
     4081 ttaaaaaaaa aaaaggaaat gaaaaaagaa caaccacctt tgcattgcat cggcttgacc
     4141 cataaactaa gttatcatgg ccagcctgcc tgctgtcgtg tgttccgcct cctccctgcc
     4201 tgcctgagaa cagtcttctt gttggggtgg gggggtccca gctgccattt gccaactgga
     4261 tgtgtgcccc acagctttct gttccccctc atgggcagca gccattatca acagcgcagc
     4321 ctgggccttc agcatctcat ttcccctgcc acccattatc ttggttttag gcatgtctaa
     4381 taactacagt cggcaccagg agagaatgtc ctgggacagg aacaatgccc cttactctca
     4441 gaggtgggac tcacaggacc aaagtttgta tctgcctgtg gtcggcatgt tgctgtctgg
     4501 tatccccttc tcccttggga cagccaggct gttttgcata caaagctgga ctagaggaag
     4561 ggaagcttag cggggtgtgg gtgagtgcag gacaggatct ccataagcag tacacctggg
     4621 aacgccacag gagagctagt tcattcatgg gggggcagga tttatatggc catggaccat
     4681 tggccggggc aggggtgttg gaagatacct catctgcatg ctcaggggca acactttata
     4741 aggtcaaaca agtgaagcct gataactgcc aggtaataaa ttctactggg gttgatgatg
     4801 gtgaggagcc aggtttatgg tgccatgttc agagggggag ggagccagtt catgcagccc
     4861 tcatgtttaa agtatccagt gccctgggcc attacggacc cacacttgtt tttgaatttt
     4921 gttttgcttt caagaatcaa attgactttt attattcata tactttgtag aaagggggat
     4981 ttaataaaca gggtagagct gtatgaagga tagggtcctt ggaggcagct agtctcttcc
     5041 ttgactggtc ttgtgtgggc caggtaacta tggttgcctg cagtggaggt gtcaagccta
     5101 aggaggtgtc attttgtaac cttccccctg acttccagct atttggggct ttctgtcccc
     5161 tcctgcagtg ttctgagcct tcaagacaat ggcatgagta agttgtgaag gctggaccca
     5221 catcttgata cttgttttct gcatcttggg cagctaagca tctgccttct gtgttcattc
     5281 accaccattc aatgtaagaa gatgcttctc agccgggcgt ggtggcgtgc gcctttgatc
     5341 ccagcacttg ggaggcagag gcaggtggat ctctgagttt aaggacagcc agggctacac
     5401 agagaaaccc tgtctcgaaa aaaagaaaaa gaaaaaaaag aaaatgcttc tctgatgaaa
     5461 attgggcacc tcttttgtct aacggtttaa ataggtattt agaaagcaac gtgttgggct
     5521 ggtgagatgg ctcagtgggt aagagcaccc gactaagttc aaatcccagc aaccacatgg
     5581 tggctcacaa ccacccgtaa tgagatctga tgccctcttc tggtgcatct gaagacagct
     5641 acagtgtact ttacatataa taaataaata aataaatctt taaaaaaaaa aaaatagaaa
     5701 gcaacgtgct actacaccag cttagttaag tccacaggga cagctatctc cacaggcctg
     5761 gcttattaga caagcttcta ataccaggca ctgacacccc catgaaggag tcttgaatcc
     5821 tttaacaact accacaacac acacacacgt caagacacta ttacacacaa aggcatatca
     5881 tggtgttcct attggtctct ttgtcctcct cttatcctgg ctaaccctcc atctcacctg
     5941 cctacatttc cctcctatgg catccttcct ttttgcctgt gtctaccatc tcatctcctt
     6001 tcagaaatgc ccttcccggg ctggtgagat ggctcagtgg gtaagagcac ccgactgctc
     6061 ttccgaaggt caggagttca aatcccagca accacatggt ggctcacaac catctgtaac
     6121 gagatctgac tccctcttct ggagtgtctg aagacagcta cagtgtactt acatataata
     6181 aatgaataaa atctttaaaa aaaaaaaaaa aaaaaaagaa atgcccttcc cttgtgccca
     6241 gcttgcatag gtactccaca ttagacactc catcctaaga gttatataag agagagcatg
     6301 cagtttgtcc ttttgggcct cggttacctc agagcaaaag agaaaaagaa agggaaatgg
     6361 gggaaggatt tgtgtgaggg gattctgggg gaggggaagg accgatattg ggatgtaaag
     6421 ggaatgaatt aatttataaa aaagaaattg gtctggcaag atggcttagc actgactgct
     6481 cttccaaagt tcaaatccca gcaaccacat ggtgactcac agccatctat aatgagatcc
     6541 aatgcctcct tctggtgtgt ctgaagactg ctacagtgta cttatgtata ataataaata
     6601 aatctttggg ccggaaaaaa aaaaaaaaaa
//
