LOCUS       NM_001403349            4802 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus O-GlcNAcase (Oga), transcript variant 3, mRNA.
ACCESSION   NM_001403349 XM_036161721
VERSION     NM_001403349.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Formichetti,S., Sadowska,A., Ascolani,M., Hansen,J., Ganter,K.,
            Lancrin,C., Humphreys,N. and Boulard,M.
  TITLE     Genetic gradual reduction of OGT activity unveils the essential
            role of O-GlcNAc in the mouse embryo
  JOURNAL   PLoS Genet 21 (1), e1011507 (2025)
   PUBMED   39787076
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Cabrera,J.T., Si,R., Tsuji-Hosokawa,A., Cai,H., Yuan,J.X.,
            Dillmann,W.H. and Makino,A.
  TITLE     Restoration of coronary microvascular function by OGA
            overexpression in a high-fat diet with low-dose
            streptozotocin-induced type 2 diabetic mice
  JOURNAL   Diab Vasc Dis Res 20 (3), 14791641231173630 (2023)
   PUBMED   37186669
  REMARK    GeneRIF: Restoration of coronary microvascular function by OGA
            overexpression in a high-fat diet with low-dose
            streptozotocin-induced type 2 diabetic mice.
REFERENCE   3  (bases 1 to 4802)
  AUTHORS   Jo,S., Pritchard,S., Wong,A., Avula,N., Essawy,A., Hanover,J. and
            Alejandro,E.U.
  TITLE     Pancreatic beta-cell hyper-O-GlcNAcylation leads to impaired
            glucose homeostasis in vivo
  JOURNAL   Front Endocrinol (Lausanne) 13, 1040014 (2022)
   PUBMED   36387851
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4802)
  AUTHORS   Abramowitz,L.K. and Hanover,J.A.
  TITLE     Chronically Elevated O-GlcNAcylation Limits Nitric Oxide Production
            and Deregulates Specific Pro-Inflammatory Cytokines
  JOURNAL   Front Immunol 13, 802336 (2022)
   PUBMED   35432339
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4802)
  AUTHORS   Muha,V., Authier,F., Szoke-Kovacs,Z., Johnson,S., Gallagher,J.,
            McNeilly,A., McCrimmon,R.J., Teboul,L. and van Aalten,D.M.F.
  TITLE     Loss of O-GlcNAcase catalytic activity leads to defects in mouse
            embryogenesis
  JOURNAL   J Biol Chem 296, 100439 (2021)
   PUBMED   33610549
  REMARK    GeneRIF: Loss of O-GlcNAcase catalytic activity leads to defects in
            mouse embryogenesis.
REFERENCE   6  (bases 1 to 4802)
  AUTHORS   Toleman,C.A., Paterson,A.J. and Kudlow,J.E.
  TITLE     The histone acetyltransferase NCOAT contains a zinc finger-like
            motif involved in substrate recognition
  JOURNAL   J Biol Chem 281 (7), 3918-3925 (2006)
   PUBMED   16356930
REFERENCE   7  (bases 1 to 4802)
  AUTHORS   Toleman,C., Paterson,A.J., Whisenhunt,T.R. and Kudlow,J.E.
  TITLE     Characterization of the histone acetyltransferase (HAT) domain of a
            bifunctional protein with activable O-GlcNAcase and HAT activities
  JOURNAL   J Biol Chem 279 (51), 53665-53673 (2004)
   PUBMED   15485860
REFERENCE   8  (bases 1 to 4802)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   9  (bases 1 to 4802)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4802)
  AUTHORS   Comtesse,N., Maldener,E. and Meese,E.
  TITLE     Identification of a nuclear variant of MGEA5, a cytoplasmic
            hyaluronidase and a beta-N-acetylglucosaminidase
  JOURNAL   Biochem Biophys Res Commun 283 (3), 634-640 (2001)
   PUBMED   11341771
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149086.4.
            
            On Mar 29, 2022 this sequence version replaced XM_036161721.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253014.3847828.1,
                                           SRR17253012.2039135.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               AC149086.4         157827-157930       c
            105-365             AC149086.4         157064-157324       c
            366-417             AC149086.4         153164-153215       c
            418-515             AC149086.4         152719-152816       c
            516-646             AC149086.4         151019-151149       c
            647-818             AC149086.4         150180-150351       c
            819-917             AC149086.4         147794-147892       c
            918-1202            AC149086.4         145814-146098       c
            1203-1361           AC149086.4         144183-144341       c
            1362-1975           AC149086.4         141526-142139       c
            1976-2150           AC149086.4         139582-139756       c
            2151-2236           AC149086.4         136317-136402       c
            2237-2341           AC149086.4         135236-135340       c
            2342-2427           AC149086.4         132481-132566       c
            2428-2620           AC149086.4         132037-132229       c
            2621-2780           AC149086.4         128739-128898       c
            2781-4802           AC149086.4         124391-126412       c
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 38.75 cM"
     gene            1..4802
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /note="O-GlcNAcase"
                     /db_xref="GeneID:76055"
                     /db_xref="MGI:MGI:1932139"
     exon            1..104
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..2917
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /EC_number="3.2.1.169"
                     /note="isoform 2 is encoded by transcript variant 3;
                     bifunctional protein NCOAT; nuclear cytoplasmic
                     O-GlcNAcase and acetyltransferase; meningioma-expressed
                     antigen 5; beta-hexosaminidase;
                     beta-N-acetylhexosaminidase;
                     N-acetyl-beta-glucosaminidase;
                     N-acetyl-beta-D-glucosaminidase; protein O-GlcNAcase;
                     meningioma expressed antigen 5 (hyaluronidase)"
                     /codon_start=1
                     /product="protein O-GlcNAcase isoform 2"
                     /protein_id="NP_001390278.1"
                     /db_xref="GeneID:76055"
                     /db_xref="MGI:MGI:1932139"
                     /translation="MVRPRVWRSSRRVASQNGLRAFGPTDRGRRGAVAGGRRMVQKES
                     QAALEERESERNANPAAASGASLEQSVAPAPGEDNPSGAGAAAVVGAAGGARRFLCGV
                     VEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTL
                     ISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNM
                     CAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVG
                     EKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPY
                     KGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVS
                     IQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVV
                     DGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPSVLTKEEEKKQPDEEPMDMVVE
                     KQEEAEHKNDNQILTEIVEAKMAEELRPMDTDKESMAESKSPEMSMQEDCIPDVAPMQ
                     TDEQTQKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLR
                     ANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSNCANRTILYDMYSYV
                     WDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLF
                     FQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGFPFQSQPDLIGDKLVGGLL
                     SLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAE
                     KIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGA
                     FCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL"
     exon            105..365
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            366..417
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            418..515
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            516..646
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            647..818
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            819..917
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1202
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1361
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1975
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2150
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2236
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2341
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2427
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2620
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2780
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     exon            2781..4802
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3204..3209
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /note="hexamer: AATAAA"
     polyA_site      3223
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
     regulatory      4785..4790
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /note="hexamer: AATAAA"
     polyA_site      4799
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
     polyA_site      4802
                     /gene="Oga"
                     /gene_synonym="Hy5; Mgea5; Ncoat"
                     /note="major polyA site"
ORIGIN      
        1 aaactccccg gccggccgca ttctcccatg ttcccctcct ccgggtgggg taatggtccg
       61 acccagagtg tggcgctcat cccggagggt ggcgagccaa aatgggctcc gggcctttgg
      121 cccaacggac cgaggccgaa ggggagcggt ggccggcggc aggaggatgg tgcagaagga
      181 gagccaggcg gcgctggagg agcgggagag cgagcgcaac gccaaccctg ccgccgcctc
      241 gggggcttcg ttggagcagt cggtagctcc cgcccccgga gaagataacc catcgggagc
      301 cggggctgcg gccgtggtcg gggcggccgg aggggcccgc aggttcctgt gcggtgtcgt
      361 ggaagggttt tatggaagac cttgggttat ggagcagaga aaagagctct ttagaaggct
      421 tcagaaatgg gaattaaaca catacttgta tgctccaaaa gatgactaca aacataggat
      481 gttttggcga gagatgtatt cagtggaaga agctgagcaa cttatgactc tgatctctgc
      541 tgcacgagaa tatgaaatag aattcatcta tgctatttcg ccaggactag atatcacttt
      601 ttctaacccc aaggaagtat caacgttgaa acgcaaattg gaccaggtct ctcagtttgg
      661 gtgcaggtcg tttgctttgc tttttgatga tattgaccat aatatgtgtg cagcagacaa
      721 agaggtgttc agttcttttg cacatgcaca agtttccatc accaatgaaa tttatcagta
      781 cctgggagag ccagaaacct tcctcttctg ccctacagaa tactgtggca ctttctgtta
      841 tccaaatgta tctcagtctc cttacttgag gactgtgggt gagaagcttc tacctggaat
      901 tgaggtgctt tggacaggtc caaaagttgt ttcaaaagaa attccagtgg aatctattga
      961 agaagtttct aagatcatta agagagctcc agtgatctgg gataacattc atgctaatga
     1021 ttatgatcag aagaggctgt ttctgggtcc ctacaaagga agatccacag agctcatccc
     1081 acggttaaag ggagtactaa ccaatccaaa ttgtgaattt gaagctaact acgttgccat
     1141 acacaccctt gccacttggt acaagtcaaa catgaacgga gtgaggaagg atgtggtgat
     1201 gactgacagt gaagacagta ctgtatcaat acagataaag ttagaaaatg aaggcagtga
     1261 cgaagacatt gagactgatg ttctctacag cccacagatg gctttaaagc tggctttaac
     1321 tgagtggctg caggagttcg gtgtccctca tcagtacagc agtaggcaag ttgcacacag
     1381 tggagctaag acaagtgtag ttgatgggac tcccttagtt gctgcaccct ctttaaatgc
     1441 cacaactgta gttacaacag tttaccagga gcccattatg agccagggag cagctttgag
     1501 tggtgaaccc tcagttctga ccaaggaaga ggaaaagaaa cagccagatg aggaacccat
     1561 ggatatggtg gtcgaaaagc aagaagaagc agagcacaag aatgacaacc agatactaac
     1621 tgagattgtt gaagcgaaga tggcagagga gttgagacca atggacacgg ataaggagag
     1681 catggctgag tccaaatccc cagagatgtc catgcaggaa gactgcattc ctgacgtggc
     1741 tcctatgcag acagatgagc aaacacagaa ggagcagttt gtgccaggtc caaatgaaaa
     1801 gcctctgtac accgcagagc ctgtgactct ggaggactta cagttactcg ctgacctgtt
     1861 ctaccttcct tacgagcacg ggcccaaagg agcgcagatg ctacgggaat tccagtggct
     1921 tcgagcaaac agcagtgttg tcagtgtcaa ctgcaaaggg aaagactcag aaaaaataga
     1981 agaatggcgg tcacgagcag ccaagtttga agagatgtgt gcactagtga tggggatgtt
     2041 cacccggctt tccaactgtg ccaacaggac catcctttat gacatgtact cctatgtgtg
     2101 ggacatcaag agtataatgt ctatggtgaa atcttttgtg cagtggttag ggtgtcgtag
     2161 tcactcttca gcacagttct taattggaga ccaggagccc tgggccttta gaggtggtct
     2221 agcaggagag ttccagcgtt tgctgccaat tgatggagca aatgatctct tttttcaacc
     2281 acccccactg actccaacct ccaaagttta tactatcaga ccttatttcc ctaaagatga
     2341 ggcttccgtg tacaagattt gcagagaaat gtatgatgat ggagtgggtt tcccttttca
     2401 aagtcaacct gaccttattg gagacaagtt agtaggagga ctgctctccc tcagcctgga
     2461 ttactgcttc gtcctagaag atgaagatgg catttgtggt tatgccttag gcacagtaga
     2521 tgtgaccccc ttcattaaaa agtgtaaaat ttcctggatt cccttcatgc aagagaagta
     2581 taccaagcca aatggtgaca aagagctctc agaggctgag aaaataatgt tgagtttcca
     2641 tgaagagcag gaggtgttgc cagaaacttt ccttgccaac ttcccttctc tgataaaaat
     2701 ggatattcat aaaaaagtga ctgacccgag tgttgccaaa agcatgatgg cttgcctcct
     2761 gtcttcactg aaggccaatg gctcccgggg ggctttctgt gaagtaagac cagatgataa
     2821 aaggattctg gaattttaca gcaagttagg ctgttttgaa attgcaaaaa tggaaggatt
     2881 tccaaaggat gtggttatac ttggtcgaag cctgtgacac ttgttggtac tgtgaactgt
     2941 ccaaaagtcc cttaactccg ccttgtggtt ggtggttgag gtcttcacac agttcagtgt
     3001 ctggagctgg agcggcagca gatcagccag ttgacttgga aacaaagatg actgtaaagc
     3061 gcacccatca ccttctcaga tgctcactgg ttgggagaag atagtattgc tgcatacccc
     3121 gtgcagcttg tggtgtgctc ctctttgggt cttgtgttag ggtcagggcc tcttacgtgg
     3181 ggctctctag ggagagattc ttaaataaat ggagtcagca ctattttctg catccagtgt
     3241 ggttgcattt ctcacctaag agcaacgaag ttcactctct ttactctcct tggtgtgctg
     3301 gatgaaatgt ctctgttctt tggagacatg gctggaatga gaaatttagt gagtttcaat
     3361 ttgggcaatt tttctgcttt ttgtaaagtt ctgggcaact gtctcctgta actgttggtt
     3421 caggccattg agctttgtta gggacaaatg tgcatttgtt tgtggccaag gcctaaattg
     3481 acaaacttgt aaatctctgt atatagatta gctgtaaccc gactgttctt tgtttactgt
     3541 tttatatatt atggggtttt tttttccttt gaaaatgaaa ggtgttggtt taggatggca
     3601 ctttgaatta tgtaaatcag tgaatggtag atttccttta catctgtcct tttcttccta
     3661 atagttttta tttttgaagt gcctcccaca tagggttagg ccatgactat ttagggtatg
     3721 agcacctaac ttcttatgac tcagatatgt tgggaagtgc agtaactcct gggaattaac
     3781 cttaatagca gatccgcatg tgaaatgttc tgacattgtt aagtaagggt aaggggactg
     3841 accagtggtc tcctttgtcc ttgaacagtt gtcatttaat cgcaaagacc tgttaactac
     3901 tggtttaatc gaatcagtcc ttaattcttg catgtttgta tctaattttt aaaagaaaga
     3961 ataagtatat gttaacaata tgtatacttt ttcttttaat cagagattct gaaaacttca
     4021 tgttatttta atttagattt ggtgaattta agggttttga gcatgaagct ggcacatagt
     4081 gtctgcagga ttattattct ttaatcatgg ctagctatct agtaacgtca tagattttca
     4141 acagtctttt gttaccttgc tttcctccag ttctacatag cttttgaact ttgagctgag
     4201 gggggagaga gaagagggag cttgaaacat tttcctttct agaactccag tgtgagcttg
     4261 tgaggtagtc ttttagcact atatagagtg gacccaggga ggcccaagaa ccagaggccc
     4321 aagagtgtgc tgaaatgtgc cgcctcctta gggtgcgaac ctttgcgata ggatgtgctg
     4381 tgaaacctaa gtcaaggttg gttttgaagg ctggggtgag gggcaaaatt gttactagat
     4441 attaagaccg tttaatgttc cttagcgttc tgaatccatc tcatgcctca gtgagaatta
     4501 caaggtttta ttcagagcgt ttcagagttt catacggaat caggaattga acaagttgct
     4561 ttttgtttaa tgttggcttt caataatcac atgggatgat cttctggaca taagtgaaaa
     4621 catgtccagg tctctactgt cattgctgta ggataaagtg ctctgccatc ttccatggtt
     4681 atgaatattt attaatgttg gaataacatt tcattgttta gatcacagct cagttcctat
     4741 agaaaacgaa ctgtaaacct atctgcagac catgcaagag gcacaataaa acttgaagtg
     4801 aa
//
