LOCUS       NM_016901                955 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus ornithine decarboxylase antizyme 3 (Oaz3), mRNA.
ACCESSION   NM_016901
VERSION     NM_016901.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Tokuhiro,K., Isotani,A., Yokota,S., Yano,Y., Oshio,S., Hirose,M.,
            Wada,M., Fujita,K., Ogawa,Y., Okabe,M., Nishimune,Y. and Tanaka,H.
  TITLE     OAZ-t/OAZ3 is essential for rigid connection of sperm tails to
            heads in mouse
  JOURNAL   PLoS Genet 5 (11), e1000712 (2009)
   PUBMED   19893612
  REMARK    GeneRIF: Results indicated that OAZ-t is essential for the
            formation of a rigid junction between the head and tail during
            spermatogenesis.
REFERENCE   2  (bases 1 to 955)
  AUTHORS   Lopez-Contreras,A.J., Sanchez-Laorden,B.L., Ramos-Molina,B., de la
            Morena,M.E., Cremades,A. and Penafiel,R.
  TITLE     Subcellular localization of antizyme inhibitor 2 in mammalian
            cells: Influence of intrinsic sequences and interaction with
            antizymes
  JOURNAL   J Cell Biochem 107 (4), 732-740 (2009)
   PUBMED   19449338
REFERENCE   3  (bases 1 to 955)
  AUTHORS   Snapir,Z., Keren-Paz,A., Bercovich,Z. and Kahana,C.
  TITLE     Antizyme 3 inhibits polyamine uptake and ornithine decarboxylase
            (ODC) activity, but does not stimulate ODC degradation
  JOURNAL   Biochem J 419 (1), 99-103 (2009)
   PUBMED   19076071
REFERENCE   4  (bases 1 to 955)
  AUTHORS   Lopez-Garcia,C., Lopez-Contreras,A.J., Cremades,A., Castells,M.T.
            and Penafiel,R.
  TITLE     Transcriptomic analysis of polyamine-related genes and polyamine
            levels in placenta, yolk sac and fetus during the second half of
            mouse pregnancy
  JOURNAL   Placenta 30 (3), 241-249 (2009)
   PUBMED   19131104
REFERENCE   5  (bases 1 to 955)
  AUTHORS   Ike,A., Ohta,H., Onishi,M., Iguchi,N., Nishimune,Y. and Nozaki,M.
  TITLE     Transient expression analysis of the mouse ornithine decarboxylase
            antizyme haploid-specific promoter using in vivo electroporation
  JOURNAL   FEBS Lett 559 (1-3), 159-164 (2004)
   PUBMED   14960325
  REMARK    GeneRIF: results define the transcriptional regulatory elements of
            the OAZt gene promoter; an initiator, a downstream element, and two
            cyclic adenosine monophosphate-responsive element (CRE)-like sites
            in the upstream region
REFERENCE   6  (bases 1 to 955)
  AUTHORS   Ike,A., Yamada,S., Tanaka,H., Nishimune,Y. and Nozaki,M.
  TITLE     Structure and promoter activity of the gene encoding ornithine
            decarboxylase antizyme expressed exclusively in haploid germ cells
            in testis (OAZt/Oaz3)
  JOURNAL   Gene 298 (2), 183-193 (2002)
   PUBMED   12426106
REFERENCE   7  (bases 1 to 955)
  AUTHORS   Ivanov,I.P., Gesteland,R.F. and Atkins,J.F.
  TITLE     Antizyme expression: a subversion of triplet decoding, which is
            remarkably conserved by evolution, is a sensor for an
            autoregulatory circuit
  JOURNAL   Nucleic Acids Res 28 (17), 3185-3196 (2000)
   PUBMED   10954585
  REMARK    Review article
REFERENCE   8  (bases 1 to 955)
  AUTHORS   Ivanov,I.P., Rohrwasser,A., Terreros,D.A., Gesteland,R.F. and
            Atkins,J.F.
  TITLE     Discovery of a spermatogenesis stage-specific ornithine
            decarboxylase antizyme: antizyme 3
  JOURNAL   Proc Natl Acad Sci U S A 97 (9), 4808-4813 (2000)
   PUBMED   10781085
  REMARK    GeneRIF: antizyme expression requires programmed, ribosomal
            frameshifting
REFERENCE   9  (bases 1 to 955)
  AUTHORS   Coffino,P.
  TITLE     Polyamines in spermiogenesis: not now, darling
  JOURNAL   Proc Natl Acad Sci U S A 97 (9), 4421-4423 (2000)
   PUBMED   10781034
  REMARK    Review article
REFERENCE   10 (bases 1 to 955)
  AUTHORS   Tosaka,Y., Tanaka,H., Yano,Y., Masai,K., Nozaki,M., Yomogida,K.,
            Otani,S., Nojima,H. and Nishimune,Y.
  TITLE     Identification and characterization of testis specific ornithine
            decarboxylase antizyme (OAZ-t) gene: expression in haploid germ
            cells and polyamine-induced frameshifting
  JOURNAL   Genes Cells 5 (4), 265-276 (2000)
   PUBMED   10792465
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC122808.3, AF175297.1 and
            AI449747.1.
            
            On Aug 15, 2014 this sequence version replaced NM_016901.2.
            
            Summary: The protein encoded by this gene belongs to the ornithine
            decarboxylase antizyme family, which plays a role in cell growth
            and proliferation by regulating intracellular polyamine levels.
            Expression of antizymes requires +1 ribosomal frameshifting, which
            is enhanced by high levels of polyamines. Antizymes in turn bind to
            and inhibit ornithine decarboxylase (ODC), the key enzyme in
            polyamine biosynthesis; thus, completing the auto-regulatory
            circuit. This gene encodes antizyme 3, the third member of the
            antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC
            activity and polyamine uptake; however, it does not stimulate ODC
            degradation. Also, while antizymes 1 and 2 have broad tissue
            distribution, expression of antizyme 3 is restricted to haploid
            germ cells in testis, suggesting a distinct role for this antizyme
            in spermiogenesis. Antizyme 3 gene knockout studies showed that
            homozygous mutant male mice were infertile, and indicated the
            likely role of this antizyme in the formation of a rigid connection
            between the sperm head and tail during spermatogenesis. This
            transcript initiates translation from a non-AUG (CUG) codon that is
            highly conserved among the antizyme 3 orthologs. [provided by
            RefSeq, Dec 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF175297.1, AK076942.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: inferred from conservation
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ribosomal slippage       :: PMID: 10792465
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC122808.3         100002-100036
            36-947              AF175297.1         1-912
            948-955             AI449747.1         1-8                 c
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 40.66 cM"
     gene            1..955
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /note="ornithine decarboxylase antizyme 3"
                     /db_xref="GeneID:53814"
                     /db_xref="MGI:MGI:1858170"
     exon            1..264
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /inference="alignment:Splign:2.1.0"
     CDS             join(98..355,357..830)
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /ribosomal_slippage
                     /note="protein translation is dependent on
                     polyamine-induced +1 ribosomal frameshift; non-AUG (CUG)
                     translation initiation codon; testis specific ornithine
                     decarboxylase antizyme; ODC-Az 3; antizyme 3"
                     /codon_start=1
                     /product="ornithine decarboxylase antizyme 3"
                     /protein_id="NP_058597.2"
                     /db_xref="CCDS:CCDS84654.1"
                     /db_xref="GeneID:53814"
                     /db_xref="MGI:MGI:1858170"
                     /translation="MPCNRSRPSLYSLSYIKRGKTRNYLYPFWSPFAYYLYCYKYRIT
                     LREKMLPCCYKSITYKEQEDLTLRPHCCLPCSCLPCSCLQCSESLGGLQVGRSTAQEK
                     DHSQLKELYSAGNLTVLSTDPLLHQDPVQLDFHFRLTPHSSAHWHGLLCDHRLFLDIP
                     YQALDQGNRESLTATLEYVEEKTNVDSVFVNFQIDRKDRGALLRAFSYMGFEVVRPDH
                     PALPPWDNVIFMVYPLERDLGHPGQ"
     exon            265..434
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /inference="alignment:Splign:2.1.0"
     exon            435..610
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /inference="alignment:Splign:2.1.0"
     exon            611..696
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /inference="alignment:Splign:2.1.0"
     exon            697..950
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /inference="alignment:Splign:2.1.0"
     regulatory      929..934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /note="hexamer: AATAAA"
     polyA_site      950
                     /gene="Oaz3"
                     /gene_synonym="Az-3; AZ3; Oaz-t"
                     /note="major polyA site"
ORIGIN      
        1 ataatgcctg ccaggtcaca atggacaggg tggcttgaac ctctgcgact tatccgctga
       61 acctcacttt gccgcaggga ggcactgaac agagaaactg ccttgtaaca ggtcccgccc
      121 ctctctctac tccctttctt atatcaagag gggaaaaaca cggaactatc tctatccatt
      181 ctggtcacca ttcgcctatt acctctactg ttacaaatac cggatcaccc tccgggagaa
      241 gatgctgcct tgttgttaca aaagcatcac ttacaaggaa caggaggacc tgactctccg
      301 gccccattgc tgcctcccgt gctcctgcct cccgtgctcc tgcctccagt gctcctgagt
      361 ccctaggagg cctccaggtg ggtaggagca ctgcacagga aaaagaccac agccagctta
      421 aagaactcta ttcagctggg aacctgacag tgctatcaac tgaccccctg cttcaccaag
      481 atccagttca gttagacttc cactttcgtc ttacccccca ttcctctgct cattggcacg
      541 gccttctgtg tgatcaccga ctcttcctgg atatcccata tcaggccttg gatcaaggca
      601 accgagaaag cttgacagca acactggagt atgtggagga gaaaaccaat gtggactctg
      661 tgtttgtgaa cttccaaatc gatcggaagg acagaggtgc cctgctgcga gcctttagct
      721 acatgggctt cgaggtggtt agaccagatc atcctgccct ccctccctgg gacaatgtca
      781 tcttcatggt gtatcccctt gaaagggacc ttggccaccc tggccagtga gcctccctaa
      841 acatgttcca tctctgtgag gggttggaaa cctcaacaca cgggactctg aggcccagga
      901 tgtgatttaa gatacttcca tcctaggaaa taaagggtag tgcaatcaaa aaaaa
//
