LOCUS       NM_001301307            1858 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ornithine decarboxylase antizyme 2 (Oaz2), transcript
            variant 2, mRNA.
ACCESSION   NM_001301307
VERSION     NM_001301307.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Ramos-Molina,B., Lambertos,A., Lopez-Contreras,A.J., Kasprzak,J.M.,
            Czerwoniec,A., Bujnicki,J.M., Cremades,A. and Penafiel,R.
  TITLE     Structural and degradative aspects of ornithine decarboxylase
            antizyme inhibitor 2
  JOURNAL   FEBS Open Bio 4, 510-521 (2014)
   PUBMED   24967154
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1858)
  AUTHORS   Murakami,Y., Suzuki,J., Samejima,K. and Oka,T.
  TITLE     Developmental alterations in expression and subcellular
            localization of antizyme and antizyme inhibitor and their
            functional importance in the murine mammary gland
  JOURNAL   Amino Acids 38 (2), 591-601 (2010)
   PUBMED   19997757
  REMARK    GeneRIF: The level of AZ2 mRNA decreased during mammary gland
            development.
REFERENCE   4  (bases 1 to 1858)
  AUTHORS   Murai,N., Shimizu,A., Murakami,Y. and Matsufuji,S.
  TITLE     Subcellular localization and phosphorylation of antizyme 2
  JOURNAL   J Cell Biochem 108 (4), 1012-1021 (2009)
   PUBMED   19725046
REFERENCE   5  (bases 1 to 1858)
  AUTHORS   Lopez-Contreras,A.J., Sanchez-Laorden,B.L., Ramos-Molina,B., de la
            Morena,M.E., Cremades,A. and Penafiel,R.
  TITLE     Subcellular localization of antizyme inhibitor 2 in mammalian
            cells: Influence of intrinsic sequences and interaction with
            antizymes
  JOURNAL   J Cell Biochem 107 (4), 732-740 (2009)
   PUBMED   19449338
REFERENCE   6  (bases 1 to 1858)
  AUTHORS   Ivanov,I.P., Gesteland,R.F. and Atkins,J.F.
  TITLE     Antizyme expression: a subversion of triplet decoding, which is
            remarkably conserved by evolution, is a sensor for an
            autoregulatory circuit
  JOURNAL   Nucleic Acids Res 28 (17), 3185-3196 (2000)
   PUBMED   10954585
  REMARK    Review article
REFERENCE   7  (bases 1 to 1858)
  AUTHORS   Wakana,S., Sugaya,E., Naramoto,F., Yokote,N., Maruyama,C., Jin,W.,
            Ohguchi,H., Tsuda,T., Sugaya,A. and Kajiwara,K.
  TITLE     Gene mapping of SEZ group genes and determination of
            pentylenetetrazol susceptible quantitative trait loci in the mouse
            chromosome
  JOURNAL   Brain Res 857 (1-2), 286-290 (2000)
   PUBMED   10700579
REFERENCE   8  (bases 1 to 1858)
  AUTHORS   Ivanov,I.P., Gesteland,R.F. and Atkins,J.F.
  TITLE     A second mammalian antizyme: conservation of programmed ribosomal
            frameshifting
  JOURNAL   Genomics 52 (2), 119-129 (1998)
   PUBMED   9782076
  REMARK    GeneRIF: The protein product is generated using polyamine-regulated
            programmed ribosomal frameshifting.
REFERENCE   9  (bases 1 to 1858)
  AUTHORS   Nilsson,J., Koskiniemi,S., Persson,K., Grahn,B. and Holm,I.
  TITLE     Polyamines regulate both transcription and translation of the gene
            encoding ornithine decarboxylase antizyme in mouse
  JOURNAL   Eur J Biochem 250 (2), 223-231 (1997)
   PUBMED   9428668
REFERENCE   10 (bases 1 to 1858)
  AUTHORS   Kajiwara,K., Nagawawa,H., Shimizu-Nishikawa,S., Ookuri,T.,
            Kimura,M. and Sugaya,E.
  TITLE     Molecular characterization of seizure-related genes isolated by
            differential screening
  JOURNAL   Biochem Biophys Res Commun 219 (3), 795-799 (1996)
   PUBMED   8645260
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK169243.1, CJ072367.1 and
            BC083114.1.
            
            Summary: The protein encoded by this gene belongs to the ornithine
            decarboxylase antizyme family, which plays a role in cell growth
            and proliferation by regulating intracellular polyamine levels.
            Expression of antizymes requires +1 ribosomal frameshifting, which
            is enhanced by high levels of polyamines. Antizymes in turn bind to
            and inhibit ornithine decarboxylase (ODC), the key enzyme in
            polyamine biosynthesis; thus, completing the auto-regulatory
            circuit. This gene encodes antizyme 2, the second member of the
            antizyme family. Like antizyme 1, antizyme 2 has broad tissue
            distribution, inhibits ODC activity and polyamine uptake, and
            stimulates ODC degradation in vivo; however, it fails to promote
            ODC degradation in vitro. Antizyme 2 is expressed at lower levels
            than antizyme 1, but is evolutionary more conserved, suggesting it
            likely has an important biological role. Studies also show
            different subcellular localization of antizymes 1 and 2, indicating
            specific function for each antizyme in discrete compartments of the
            cell. Alternatively spliced transcript variants have been found for
            this gene. [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (2) uses an alternate, in-frame
            acceptor splice site at the second exon compared to variant 1. The
            resulting isoform (2) is one amino acid shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            ribosomal slippage :: PMID: 9782076
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AK169243.1         1-31
            32-421              CJ072367.1         2-391
            422-1840            AK169243.1         425-1843
            1841-1858           BC083114.1         1802-1819
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 35.45 cM"
     gene            1..1858
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /note="ornithine decarboxylase antizyme 2"
                     /db_xref="GeneID:18247"
                     /db_xref="MGI:MGI:109492"
     exon            1..218
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /inference="alignment:Splign:2.1.0"
     CDS             join(199..291,293..766)
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /ribosomal_slippage
                     /note="protein translation is dependent on
                     polyamine-induced +1 ribosomal frameshift; isoform 2 is
                     encoded by transcript variant 2; ODC-Az 2; seizure-related
                     protein 15; antizyme 2"
                     /codon_start=1
                     /product="ornithine decarboxylase antizyme 2 isoform 2"
                     /protein_id="NP_001288236.1"
                     /db_xref="GeneID:18247"
                     /db_xref="MGI:MGI:109492"
                     /translation="MINTQDSILPLSKCPQLQCCRHIVPGPLWCSDAPHPLSKIPGGR
                     GGGRDPSLSALIYKDEKLTVTQDLPVNDGKPHIVHFQYEVTEVKVSSWDAVLSSQSLF
                     VEIPDGLLADGSKEGLLALLEFAEEKMKVNYVFICFRKGREDRAPLLKTFSFLGFEIV
                     RPGHPCVPSRPDVMFMVYPLDQNLSDED"
     exon            219..373
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      298..347
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /note="recoding_pseudoknot"
                     /function="stimulates +1 ribosomal frameshifting"
     exon            374..549
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /inference="alignment:Splign:2.1.0"
     exon            550..635
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /inference="alignment:Splign:2.1.0"
     exon            636..1841
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1820..1825
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /note="hexamer: AATAAA"
     polyA_site      1838
                     /gene="Oaz2"
                     /gene_synonym="AZ-2; AZ2; Oaz2-ps; Sez15"
                     /note="major polyA site"
ORIGIN      
        1 ggcggttgcg cggcggcggc cggggagggt cagttggagg caggcgctcg ctgaggcaag
       61 aggagggcac tcggcccgcg gcctgacagg gacttagccc acagagaccg gcccgcgcgc
      121 gcgaccccac acccacccac tcgtccacct acccactccc cgcgccgcct cctcccaccc
      181 tgagcagagc caccgaggat gataaacacc caggacagta ttttgccgtt gagtaagtgt
      241 ccccagctcc agtgctgcag gcacattgtt ccagggcctc tgtggtgctc ctgatgcccc
      301 tcacccactg tcgaagatcc ccggtgggcg agggggcggc agggatcctt ctctctcagc
      361 tctaatatat aaggacgaga agctcactgt aacccaggac ctccctgtga acgatggaaa
      421 acctcacatc gtccacttcc agtatgaggt caccgaggtg aaggtctctt cctgggatgc
      481 agtcctgtcc agccagagcc tgtttgtaga aatcccagat ggattattag ctgatgggag
      541 caaagaagga ttattagcac tgctagagtt tgctgaggag aagatgaaag tgaactacgt
      601 cttcatctgc ttcaggaagg gccgggaaga cagagctcca ctcctgaaga cgttcagctt
      661 cttgggcttt gagattgtgc gtccaggtca tccctgtgtc ccctctcggc cagatgtgat
      721 gtttatggtt taccccctgg accagaactt gtctgatgag gactaatggt cacagaggat
      781 gctttgccta agagccacag tgggggaaga ggggaagtta ggcagccttg ggacagagga
      841 gagggtctct ggctgtctag ggaaggacat tgaggggctc aggttgaggg ttgcccattg
      901 tgttctcgga gttgactcgt tgaaattgtt ttccataaag aacagtataa gcatattatt
      961 cacatgtaat caccaatagt aaatgaagat gtttatgaac tggcattaga agctttttaa
     1021 actgcgctgt gatgtgctcc atctacccta ggggaggaca ttgcctgcag gggcgggact
     1081 gccgggccca ggacaggcct ggagggctgg tgggcaggac tgtcaggctc aggtctcctt
     1141 ggagttgggc tttcttcttt tggttttgaa gacttaggta ttttctttct ttgcttcttg
     1201 tcatctaggg gctcctgagt ataggcttta gctcctgggc agtgtccttg ggttgtcttc
     1261 aacactccac cttgagcttc tggcattttg tgtctggcct ggtgtaagca ggtctgacct
     1321 tgctttcttt acagcttagt gttattctgg catttggtta agctggctta atctgtttaa
     1381 tgttaccagt gcattttaaa taggggcatt gaaactcact cccccacacc agggcttttt
     1441 ggggggtgcc tgggccttaa gaaactagcc aaactacatc tcaagttacc accagagctc
     1501 ttgcctctgt ggcctaggcc ccaggatctc tttccttgca agagcagaga ggaagaatac
     1561 caaactcagg gcctggggtt tgtgggggag cctgttctta gcgcctggat gcctctggct
     1621 ggccaagcac agtactgcct gcctccttaa caggttcaga acagtgcctg cgctgcagag
     1681 ctgagaggta gggctggact gcagaaatgg cctgcctttt actgctttca ccaaacagct
     1741 tgttctaaag agaaaaggga gagagatcta cattgggtgt gggggtgtca gggagacaag
     1801 tgtgttgtgt tactgtgtca ataaactgat ttaaagttgt gaaaaaaaaa aaaaaaaa
//
