LOCUS       NM_133851               2146 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus nucleolar and spindle associated protein 1 (Nusap1),
            transcript variant 1, mRNA.
ACCESSION   NM_133851
VERSION     NM_133851.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Hashimoto,K., Ohira,M., Kodama,A., Kimoto,M., Inoue,M., Tone,S.,
            Usui,Y., Hanashima,A., Goto,T., Ogura,Y., Ujihara,Y. and Mohri,S.
  TITLE     Loss of connectin novex-3 leads to heart dysfunction associated
            with impaired cardiomyocyte proliferation and abnormal nuclear
            mechanics
  JOURNAL   Sci Rep 14 (1), 13727 (2024)
   PUBMED   38877142
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Li,C., Zhang,Y., Shen,J., Bao,H., Zhao,Y., Li,D., Li,S., Liu,Y.,
            Yang,J., Zhou,Z., Gao,K., Zhao,L., Pei,Y., Lu,Y., Pan,Z. and Cai,B.
  TITLE     Cfp1 Controls Cardiomyocyte Maturation by Modifying Histone H3K4me3
            of Structural, Metabolic, and Contractile Related Genes
  JOURNAL   Adv Sci (Weinh) 11 (11), e2305992 (2024)
   PUBMED   38196272
REFERENCE   3  (bases 1 to 2146)
  AUTHORS   Yu,L., Kong,N., Lin,Y., Qiu,P., Xu,Q., Zhang,Y., Zhen,X., Yan,G.,
            Sun,H., Mei,J. and Cao,G.
  TITLE     NUSAP1 regulates mouse oocyte meiotic maturation
  JOURNAL   J Cell Biochem 124 (12), 1931-1947 (2023)
   PUBMED   37992207
  REMARK    GeneRIF: NUSAP1 regulates mouse oocyte meiotic maturation.
REFERENCE   4  (bases 1 to 2146)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2146)
  AUTHORS   Grundl,M., Walz,S., Hauf,L., Schwab,M., Werner,K.M., Spahr,S.,
            Schulte,C., Maric,H.M., Ade,C.P. and Gaubatz,S.
  TITLE     Interaction of YAP with the Myb-MuvB (MMB) complex defines a
            transcriptional program to promote the proliferation of
            cardiomyocytes
  JOURNAL   PLoS Genet 16 (5), e1008818 (2020)
   PUBMED   32469866
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2146)
  AUTHORS   Ribbeck,K., Raemaekers,T., Carmeliet,G. and Mattaj,I.W.
  TITLE     A role for NuSAP in linking microtubules to mitotic chromosomes
  JOURNAL   Curr Biol 17 (3), 230-236 (2007)
   PUBMED   17276916
REFERENCE   7  (bases 1 to 2146)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2146)
  AUTHORS   Raemaekers,T., Ribbeck,K., Beaudouin,J., Annaert,W., Van Camp,M.,
            Stockmans,I., Smets,N., Bouillon,R., Ellenberg,J. and Carmeliet,G.
  TITLE     NuSAP, a novel microtubule-associated protein involved in mitotic
            spindle organization
  JOURNAL   J Cell Biol 162 (6), 1017-1029 (2003)
   PUBMED   12963707
REFERENCE   9  (bases 1 to 2146)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2146)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844536.13.
            
            On Jan 10, 2022 this sequence version replaced NM_133851.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC100392.1, AF305710.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-167               AL844536.13        80367-80533
            168-236             AL844536.13        85756-85824
            237-383             AL844536.13        89185-89331
            384-552             AL844536.13        91975-92143
            553-651             AL844536.13        95358-95456
            652-761             AL844536.13        97116-97225
            762-877             AL844536.13        100086-100201
            878-1035            AL844536.13        101215-101372
            1036-1152           AL844536.13        105422-105538
            1153-1261           AL844536.13        108728-108836
            1262-2146           AL844536.13        110531-111415
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.97 cM"
     gene            1..2146
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /note="nucleolar and spindle associated protein 1"
                     /db_xref="GeneID:108907"
                     /db_xref="MGI:MGI:2675669"
     exon            1..167
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1358
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /note="isoform a is encoded by transcript variant 1;
                     nucleolar protein ANKT"
                     /codon_start=1
                     /product="nucleolar and spindle-associated protein 1
                     isoform a"
                     /protein_id="NP_598612.1"
                     /db_xref="CCDS:CCDS16605.1"
                     /db_xref="GeneID:108907"
                     /db_xref="MGI:MGI:2675669"
                     /translation="MTVPSAEELDSFKYSDLQNLAKRLGLRANMKADKLLKALKAHLN
                     PETRKENKNQDENQFSTDETEIHVSSEEQAETESGGHVTKTRRRRRKKHKTIHGIPTS
                     QTLLQDHLEMKGTDSSNFQNQENQENQDPRDTAEVPSLPEQRPEDGNAASSGEGEVND
                     IKDSKKPLEKRSLCTDEFSKLGNNKRTSATTPNFKKLHEARFKKMESIDEYIMRKKKH
                     LKEHSSLNELKLDKKGIVTPVPPRGRLSVPCTPARQQCPQGHSATKMNVRFSAATKDN
                     EHKCSLTKTPARKSPHVTAPGSASKGQAVFRTPKSKATERTSIAVITPFKLMTEATQT
                     PSSSKKPVFDLKASLSRPLNYKPHKGKLKPWGQAKENNSLNERVSRVTFHRKTYKQPH
                     LQTREERWKRQEQERKEKKEKLLEARRNLGVTKAQ"
     exon            168..236
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            237..383
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            384..552
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            553..651
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            652..761
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            762..877
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1035
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1152
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1261
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..2146
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2127..2132
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /note="hexamer: AATAAA"
     polyA_site      2146
                     /gene="Nusap1"
                     /gene_synonym="2610201A12Rik; ANKT; BM037; LNP; NuSAP;
                     Q0310; SAPL"
                     /note="major polyA site"
ORIGIN      
        1 ggcgccagtt ttgagccgct ctgttttgag ttgtgtggct gtttttctgg gaatcaccga
       61 gattgcagaa cgcgatgacc gtcccctctg cagaggagct ggactccttt aagtacagcg
      121 acctgcaaaa tttagccaaa aggctgggcc tccgggctaa catgaaggca gacaagttgt
      181 taaaagcctt gaaagcacac ctgaatccag aaacaaggaa agaaaataaa aatcaggatg
      241 aaaatcaatt ctccactgat gaaactgaga tacacgttag cagtgaggag caagctgaga
      301 cggaatcagg tggtcacgtc accaaaacga ggaggaggag gaggaagaag cacaagacca
      361 tccatggaat tcctacctcc cagactttgt tgcaggatca tttggagatg aaaggaactg
      421 atagtagtaa cttccaaaat caagaaaatc aggaaaatca agaccccagg gatacagcag
      481 aagttccttc tctgccggag cagaggccag aggacggcaa tgcggcttct tcaggagaag
      541 gagaagtaaa tgacattaaa gattcaaaga agcctttaga aaaaagatct ctatgcacgg
      601 atgagttttc taaacttggg aacaataaaa ggacttcagc cacaacacca aactttaaga
      661 agcttcatga ggctcgtttt aagaaaatgg aatccattga tgaatatatt atgaggaaaa
      721 agaaacacct taaagaacac agttcactta atgaactaaa gcttgacaaa aaagggatag
      781 tgaccccagt tcctccaaga ggaaggctct ctgtaccctg tactcctgcc aggcagcagt
      841 gcccacaagg ccactcagca actaaaatga atgtcaggtt ttcagctgct actaaagaca
      901 atgaacataa gtgctcactg accaagacac cagccagaaa gtctccacac gtgactgcac
      961 ctgggagtgc ttcaaaaggc caggctgtgt tcaggacacc caagtcaaag gccactgaaa
     1021 ggacttctat tgcagttatt acccctttca agttgatgac tgaagcaaca cagactccaa
     1081 gttctagtaa gaagccagta tttgatctca aagcaagctt gtctcgtccc ctcaactaca
     1141 agccacacaa aggaaagctg aaaccttggg gacaagctaa agagaacaat tctctgaacg
     1201 aacgtgtaag cagagttacc ttccacagga aaacttacaa acaacctcat ctccaaacca
     1261 gggaagaacg atggaagaga caagagcaag aacgaaagga gaagaaagaa aagcttttgg
     1321 aagctcgaag aaacctgggt gtgactaaag cccagtgacc ctgtctgttc ctttactcta
     1381 acttgttttc cttttgtatg ttttttactc tttctctact tcagtcaaaa gctcttttct
     1441 atcataactt ttggtcataa tttgtgtagt gtctcttctg tgctatatct gagaatatat
     1501 tatcacctta aagttcataa ctaaagtagt tttcatctaa tgccctatac atctccaatt
     1561 tttaaaagac ttgtcctcat gattcttaac agaggttttt catgtcaagg tcctgatagt
     1621 tccgagggag atgacctgca cccttttctg cacaagctgt agcagaatct tagcatgaga
     1681 aaataagata tcgctcatag gaaaagggag gctggaagac cacatttttg ttcagtagcc
     1741 tggaaaatct attagtctta ttgaaatctt attttatcaa ggtaaaaagt tttagcttat
     1801 aagagccttg ttctgacttt tcatgtattt gccatctgtc attcattata atcctaaacg
     1861 agaaaacctc tactctctct acttgttaaa taaaagccac agggcaaagg gtgtgcttta
     1921 gttgtacaac acttgtctat cacactcctg tccctgaact gtaccaccaa aaccaaagct
     1981 gagaattgct gctgtaagaa ttactgtcat tggcagactt ttttcttaca agtagtaaag
     2041 agaggaaagc tgcaaggggt gaccttctga tctttgctct gccttacaga gattctgaga
     2101 tgtgtgtaat gcttacaatg ttcataaata aaaattttca ctgtga
//
