LOCUS       NM_030250               4614 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus NUS1 dehydrodolichyl diphosphate synthase subunit
            (Nus1), mRNA.
ACCESSION   NM_030250 XM_911273
VERSION     NM_030250.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Gong,K., Wang,M., Wang,D., Gao,Y., Ma,L., Yang,X., Zhu,X., Chen,S.,
            Zhang,M., Li,H., Chen,Y., Hu,W., Miao,Q.R., Iwakiri,Y., Liao,C.,
            Duan,Y. and Han,J.
  TITLE     Overexpression of NgBR inhibits high-fat diet-induced
            atherosclerosis in ApoE-deficiency mice
  JOURNAL   Hepatol Commun 7 (4), e0048 (2023)
   PUBMED   36996002
  REMARK    GeneRIF: Overexpression of NgBR inhibits high-fat diet-induced
            atherosclerosis in ApoE-deficiency mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Fang,Z., Sun,X., Wang,X., Ma,J., Palaia,T., Rana,U., Miao,B.,
            Ragolia,L., Hu,W. and Miao,Q.R.
  TITLE     NOGOB receptor deficiency increases cerebrovascular permeability
            and hemorrhage via impairing histone acetylation-mediated CCM1/2
            expression
  JOURNAL   J Clin Invest 132 (9) (2022)
   PUBMED   35316220
REFERENCE   3  (bases 1 to 4614)
  AUTHORS   Chen,Y., Hu,W., Li,Q., Zhao,S., Zhao,D., Zhang,S., Wei,Z., Yang,X.,
            Chen,Y., Li,X., Liao,C., Han,J., Miao,Q.R. and Duan,Y.
  TITLE     NGBR is required to ameliorate type 2 diabetes in mice by enhancing
            insulin sensitivity
  JOURNAL   J Biol Chem 296, 100624 (2021)
   PUBMED   33812996
REFERENCE   4  (bases 1 to 4614)
  AUTHORS   Zhang,W., Yang,X., Chen,Y., Hu,W., Liu,L., Zhang,X., Liu,M.,
            Sun,L., Liu,Y., Yu,M., Li,X., Li,L., Zhu,Y., Miao,Q.R., Han,J. and
            Duan,Y.
  TITLE     Activation of hepatic Nogo-B receptor expression-A new anti-liver
            steatosis mechanism of statins
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1863 (2), 177-190 (2018)
   PUBMED   29217477
REFERENCE   5  (bases 1 to 4614)
  AUTHORS   Zhao,B., Hu,W., Kumar,S., Gonyo,P., Rana,U., Liu,Z., Wang,B.,
            Duong,W.Q., Yang,Z., Williams,C.L. and Miao,Q.R.
  TITLE     The Nogo-B receptor promotes Ras plasma membrane localization and
            activation
  JOURNAL   Oncogene 36 (24), 3406-3416 (2017)
   PUBMED   28068323
  REMARK    GeneRIF: Data demonstrate that NgBR is a unique receptor that
            promotes accumulation of prenylated Ras at the plasma membrane and
            promotes EGF pathways.
REFERENCE   6  (bases 1 to 4614)
  AUTHORS   Park,E.J., Grabinska,K.A., Guan,Z., Stranecky,V., Hartmannova,H.,
            Hodanova,K., Baresova,V., Sovova,J., Jozsef,L., Ondruskova,N.,
            Hansikova,H., Honzik,T., Zeman,J., Hulkova,H., Wen,R., Kmoch,S. and
            Sessa,W.C.
  TITLE     Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase,
            causes a congenital disorder of glycosylation
  JOURNAL   Cell Metab 20 (3), 448-457 (2014)
   PUBMED   25066056
  REMARK    GeneRIF: Loss of Nogo-B receptor in mice causes early embryonic
            lethality and defects in glycosylation.
REFERENCE   7  (bases 1 to 4614)
  AUTHORS   Harrison,K.D., Miao,R.Q., Fernandez-Hernando,C., Suarez,Y.,
            Davalos,A. and Sessa,W.C.
  TITLE     Nogo-B receptor stabilizes Niemann-Pick type C2 protein and
            regulates intracellular cholesterol trafficking
  JOURNAL   Cell Metab 10 (3), 208-218 (2009)
   PUBMED   19723497
REFERENCE   8  (bases 1 to 4614)
  AUTHORS   Li,M. and Song,J.
  TITLE     Nogo-B receptor possesses an intrinsically unstructured ectodomain
            and a partially folded cytoplasmic domain
  JOURNAL   Biochem Biophys Res Commun 360 (1), 128-134 (2007)
   PUBMED   17585875
  REMARK    GeneRIF: Our results indicate that the NgBR ectodomain is
            intrinsically unstructured without tertiary structures while the
            cytoplasmic domain is partially folded with secondary structures
            but without a tight tertiary packing [nogo-b receptor]
REFERENCE   9  (bases 1 to 4614)
  AUTHORS   Miao,R.Q., Gao,Y., Harrison,K.D., Prendergast,J., Acevedo,L.M.,
            Yu,J., Hu,F., Strittmatter,S.M. and Sessa,W.C.
  TITLE     Identification of a receptor necessary for Nogo-B stimulated
            chemotaxis and morphogenesis of endothelial cells
  JOURNAL   Proc Natl Acad Sci U S A 103 (29), 10997-11002 (2006)
   PUBMED   16835300
  REMARK    GeneRIF: identify a previously uncharacterized Nogo-B receptor
            specific for the amino terminus of Nogo-B.
REFERENCE   10 (bases 1 to 4614)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153526.13.
            
            On Aug 13, 2009 this sequence version replaced NM_030250.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155620.1, BC018372.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-622               AC153526.13        99010-99631
            623-748             AC153526.13        110656-110781
            749-898             AC153526.13        111530-111679
            899-998             AC153526.13        114808-114907
            999-4614            AC153526.13        118040-121655
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 26.64 cM"
     gene            1..4614
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /note="NUS1 dehydrodolichyl diphosphate synthase subunit"
                     /db_xref="GeneID:52014"
                     /db_xref="MGI:MGI:1196365"
     exon            1..622
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /inference="alignment:Splign:2.1.0"
     CDS             196..1089
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /EC_number="2.5.1.87"
                     /note="Nogo-B receptor; ngBR;
                     di-trans,poly-cis-decaprenylcistransferase;
                     dehydrodolichyl diphosphate syntase complex subunit Nus1;
                     nuclear undecaprenyl pyrophosphate synthase 1 homolog"
                     /codon_start=1
                     /product="dehydrodolichyl diphosphate synthase complex
                     subunit Nus1"
                     /protein_id="NP_084526.1"
                     /db_xref="CCDS:CCDS23841.1"
                     /db_xref="GeneID:52014"
                     /db_xref="MGI:MGI:1196365"
                     /translation="MTGLYELVWRVLHALLCLHLTLTSWLRVRFGTWNWIWRRCCRAA
                     SAAVLAPLGFTLRKPRAVGRNRRHHRHPHGGPGPGPGPAATHPRLRWRADVRSLQKLP
                     VHMGLLVTEEVQEPSFSDIASLVVWCMAVGISYISVYDHQGIFKRNNSRLMDEILKQQ
                     QELLGQDCSKYSAEFANSNDKDDQDLNCPSAVKVLSPEDGKADIVRAAQDFCQLVAQQ
                     QRKPTDLDVDLLGSLLSSHGFPDPDLVLKFGPVDSTLGFLPWQIRLTEIVSLPSHLNI
                     SYEDFFSALRQYAACEQRLGK"
     exon            623..748
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /inference="alignment:Splign:2.1.0"
     exon            749..898
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /inference="alignment:Splign:2.1.0"
     exon            899..998
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /inference="alignment:Splign:2.1.0"
     exon            999..4614
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1167..1172
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /note="hexamer: TATAAA"
     polyA_site      1188
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
     regulatory      2412..2417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /note="hexamer: AATAAA"
     polyA_site      2431
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /note="major polyA site"
     regulatory      3602..3607
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /note="hexamer: AATAAA"
     polyA_site      3628
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
     regulatory      4581..4586
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
                     /note="hexamer: AATAAA"
     polyA_site      4605
                     /gene="Nus1"
                     /gene_synonym="1600027K07Rik; D10Ertd438e"
ORIGIN      
        1 gggggcgggg ctgcggaggg cggaggaaga tggcggcggg ggctaggtga ggtgttggca
       61 gtggcgaggg gctcgggcgc ggggggtggg gggacgcgga gcgatggccc gcgccggccg
      121 ctggggcgga taagaccctg tcgcgccgcg ggtgtgggcc ggagggaggt gacggtgccc
      181 gagcgccaca ggagtatgac ggggctgtac gagctggtgt ggcgggtgct gcacgcgctg
      241 ctctgcctgc acctcacgct cacctcctgg ctccgcgttc gcttcggcac ctggaactgg
      301 atctggcggc gctgctgtcg cgccgcctcc gccgcggtcc tagcgccgct cggcttcacg
      361 ctccgcaagc cccgggccgt cggcaggaac cggcgtcatc accggcaccc gcacggggga
      421 ccgggaccgg gaccgggacc ggccgccacc catcctcggc tgcgctggcg cgcggacgtc
      481 cggtccctgc agaagctgcc ggtgcacatg ggcctgttgg tcacggagga agtgcaggag
      541 cccagcttct cagacatcgc cagcctcgtg gtgtggtgta tggccgtggg gatctcctac
      601 attagcgtct acgaccacca aggtattttc aagagaaata attccagatt gatggatgaa
      661 attttaaaac agcaacagga acttttgggc caagattgtt ccaaatactc agcagagttt
      721 gcaaatagta atgacaaaga cgatcaagat ttaaattgcc cttcggcagt gaaggtgctg
      781 tccccagaag atggaaaagc ggatattgtg agagctgccc aggatttttg ccagttagta
      841 gcccagcagc agaggaagcc cacagatctg gatgtagatc tgctaggcag cttacttagt
      901 tcacatgggt tccctgatcc tgacttagtg ttgaagtttg gtcctgtgga cagcacatta
      961 ggctttcttc cctggcaaat cagattgact gagatcgtct ctctgccttc tcacctaaac
     1021 atcagctatg aggacttctt ctccgccctt cggcagtatg cagcttgcga acagcgactg
     1081 ggaaagtaat ggtccctact tgcataattt gacttcaggc ttttggagaa aaaggaccca
     1141 agtgacactg atgtttacaa agtacctata aaaccctgta cacacctagt tcatattcct
     1201 cataatttat caacaaacac aaaaaagtgt cttacttgag agcgaatgag tgtgtgtgtg
     1261 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tggaaataaa tttataaatg ggggcacatt
     1321 ggagaatgga gtatatagac tggagttcaa gcaagtaaca gcagtgtgtg tcttaggaac
     1381 tgagattcca gatagagagg cttcggttcc tgctacttcc ctgtttgtct ggggagtaga
     1441 ggaatgagta gaactgattt agttgtctgt ggtgggtggg gataattttt gtgcagtctt
     1501 ttttagtgac caaactttaa ttttgaagaa gaatatattg acttactaag gggaagtatt
     1561 ctcattttga gtgacattca gaggttcacg tttgcttctc tgccgagcaa agggaacgtc
     1621 ctcagatttc cgtccatttc atttcttcat tagagtaaac agtgggactt gggaggatta
     1681 gtctggtgtc tattctgggc tagagagatc atttgacgaa aaaaaactta cactatttga
     1741 tgtacagaat agaaaacatt taaatattga attaggcact caaaaccaac aaagagaatg
     1801 agcaatcatc ttaaagatgg aacctcttaa aggacaactt tgatttcctt cttcgtgtga
     1861 agatgctgga tagtacccaa atcacttaaa acctgtttcc attcgctttt tacttaaaaa
     1921 taaacatttg cgagaagtta ccaaggcagc tcttgtcctc agaaggaacc tgctcctctt
     1981 gagtggcacc tgggattttc tcctcccagt aaatctcagt gatgatgtca tggtgattga
     2041 gtaggatgag gcctgtatta ccgaagtctg ggagcataat tgaacttggt cttctgtgtt
     2101 ctagagaaca gcactgctct gacaagtgaa ttcttgataa atggctaatg cttttctctc
     2161 acactgctaa tctttggatg gaaaccattc tttatattga tggacagttt tcagaaaact
     2221 atcaacaggt gtacaagagt catgttcatt tagaatagag cagtgtaaat gctaggcctc
     2281 agaaatctga aaaaagatag caaagaaagt gggtttggaa cgtatggttt ggaattgctt
     2341 gtcagactct gaagaagatg tgtttcagct tgggattaca aaccccattt ataattttta
     2401 aatatacttg aaataaaatg attaaaatga attttggtcc agtcacatta ctttgcactt
     2461 cttcttcttt tttttctttt tgtacaaaat tttaaagact taatttattg ccaaaattct
     2521 gtgtaaagtg acagtgtatc taacagttgt tagagaactc cccatttctg ttcccctgtg
     2581 ctgtgtatgg tctgtgttcc tctggtttga ttccctctgg cggtggggga ggggcggagt
     2641 tgtgtgtaat ttaagacaat ttaaggtctt ttctaaacac gatagttata aatgtccttt
     2701 ttagatcttc tggggtatgc acagctttaa tgaattaaat ttactggaat caattgaaat
     2761 aagaggactc aaggtttgtt ctaatgaagt ttgtcaaatt actctttgga attgagccaa
     2821 aatcctagag ttgagttttt attcttcctt ccttccatac aacaaaacaa gtccatagcc
     2881 tatgacccaa gactgtttgt ggccttagga acaattgctt aagcaggtca agcattttga
     2941 atttcaatgt aggccttcag gatcttctca catgaggtca actgctaatg caatctctac
     3001 acttcaaaaa gcactgatcg ttctccagtt cttgccaaga tcatcccaag ttggatatca
     3061 tcaaaactat ctttgtgctc ccaaaacttg gtgttcatgt gtatcccttt aagttgtttc
     3121 tccttgacac gctccacaat ggcaaagata ccaagtcata cctactcaac agatggcatg
     3181 ggacatatga agaaatggag acattaactt cgttctaaag cctgattgta gattgctcct
     3241 taaaactgaa cagttagtgt ttgaagttga aatgctgaat ataatgtgaa tagcaaaatc
     3301 tggtatgttg tgactaaggc ttaagagtgc ctgtttctga caataaaatg ttccaatatt
     3361 aatgaacagt gccaaataca ctgtgcatat ctataatttt ccatttgaat gtatgttaat
     3421 tactcccttc tatgtgatgg tcccatgctt agagtcagtg aagttcttgt gatgtttgtt
     3481 atgcttgtag attttggcaa cttgtaaata atgtgtatac caagttttta tgattaagga
     3541 atcaaattta ttgaatttta ttattgaaag ttgaaataag atgtatgaac aaaaaaaaat
     3601 caataaaaga atatactctt ttcatggact gtagtagtat gtgaatgtta catttagttt
     3661 gaacagttct gaagtataag gagaaatgta tgaccagata gcaatcctgc ttttagatag
     3721 tatgctcttg attggaacaa attttcattt ttttaaaaat ctgctaaaca tttgcaatct
     3781 tacatttgta ttttaatacc agagtgaggt ttagctttgt ctttctggga ttctcaggtt
     3841 ccacagtaat tacaagctga ctttttactg aacataggaa tgttaaaaac aaatgtgtag
     3901 gtggctggag aaatggctta gtggttaaga gctcggtcct gagttcaatt cccagcaacc
     3961 acatggtggc tcatagccat ctgtaatggg atttggtgcc ctcttctggt gtgtctgaaa
     4021 acagctacag catacccata taaataaaat aaatatatct ttacaaaaaa acccaccatt
     4081 ctcaaaaaga aaaacaaaaa gaatgtagct ttttaagaac tactttaatt gaaagataag
     4141 aactttgcta tcttatgcag tgtagtgttc cctttctgaa gacctcattg taagacagct
     4201 ttatgtgcat agtgctcatc tgtagctagg gcacaccaaa ggtcatattt taactcttta
     4261 taggaaatgc ctttaacaca agtgactgta ggtgactttc ttgtgcagta ttgtgactcc
     4321 caagagtgac tgtaggatgg tttgaacgaa aacccagggc cactgatagc agtgcccaag
     4381 attatctgta aagtaataaa atctgccgag gagaatgtga aaagaatttg ttcaaggctt
     4441 aacatttttg atacagacta ataaaattta agaaacaaag gattctggga acagacagga
     4501 agggccctag gttacatttt tggtgatgat cctctcagaa cttttgtaat tttattttaa
     4561 aatgtgtata tatcaggctt aataaagata ctcttttaag ctatacacct agaa
//
