LOCUS       NM_016714               4766 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus nucleoporin 50 (Nup50), mRNA.
ACCESSION   NM_016714
VERSION     NM_016714.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Park,E., Lee,B., Clurman,B.E. and Lee,K.
  TITLE     NUP50 is necessary for the survival of primordial germ cells in
            mouse embryos
  JOURNAL   Reproduction 151 (1), 51-58 (2016)
   PUBMED   26515778
  REMARK    GeneRIF: results suggest that nucleoporin 50 plays an essential
            role in the survival of primordial germ cells during embryonic
            development
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Buchwalter,A.L., Liang,Y. and Hetzer,M.W.
  TITLE     Nup50 is required for cell differentiation and exhibits
            transcription-dependent dynamics
  JOURNAL   Mol Biol Cell 25 (16), 2472-2484 (2014)
   PUBMED   24943837
  REMARK    GeneRIF: Results suggest a transport-independent role for Nup50 in
            chromatin biology that occurs away from the NPC.
REFERENCE   3  (bases 1 to 4766)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
REFERENCE   4  (bases 1 to 4766)
  AUTHORS   Maggi,L.B. Jr., Kuchenruether,M., Dadey,D.Y., Schwope,R.M.,
            Grisendi,S., Townsend,R.R., Pandolfi,P.P. and Weber,J.D.
  TITLE     Nucleophosmin serves as a rate-limiting nuclear export chaperone
            for the Mammalian ribosome
  JOURNAL   Mol Cell Biol 28 (23), 7050-7065 (2008)
   PUBMED   18809582
REFERENCE   5  (bases 1 to 4766)
  AUTHORS   Lupu,F., Alves,A., Anderson,K., Doye,V. and Lacy,E.
  TITLE     Nuclear pore composition regulates neural stem/progenitor cell
            differentiation in the mouse embryo
  JOURNAL   Dev Cell 14 (6), 831-842 (2008)
   PUBMED   18539113
REFERENCE   6  (bases 1 to 4766)
  AUTHORS   Muller,D., Thieke,K., Burgin,A., Dickmanns,A. and Eilers,M.
  TITLE     Cyclin E-mediated elimination of p27 requires its interaction with
            the nuclear pore-associated protein mNPAP60
  JOURNAL   EMBO J 19 (10), 2168-2180 (2000)
   PUBMED   10811608
REFERENCE   7  (bases 1 to 4766)
  AUTHORS   Onodera,K., Shavit,J.A., Motohashi,H., Katsuoka,F., Akasaka,J.E.,
            Engel,J.D. and Yamamoto,M.
  TITLE     Characterization of the murine mafF gene
  JOURNAL   J Biol Chem 274 (30), 21162-21169 (1999)
   PUBMED   10409670
REFERENCE   8  (bases 1 to 4766)
  AUTHORS   Yang,W. and Mansour,S.L.
  TITLE     Expression and genetic analysis of prtb, a gene that encodes a
            highly conserved proline-rich protein expressed in the brain
  JOURNAL   Dev Dyn 215 (2), 108-116 (1999)
   PUBMED   10373015
REFERENCE   9  (bases 1 to 4766)
  AUTHORS   Tate,P., Lee,M., Tweedie,S., Skarnes,W.C. and Bickmore,W.A.
  TITLE     Capturing novel mouse genes encoding chromosomal and other nuclear
            proteins
  JOURNAL   J Cell Sci 111 (Pt 17), 2575-2585 (1998)
   PUBMED   9701556
REFERENCE   10 (bases 1 to 4766)
  AUTHORS   Fan,F., Liu,C.P., Korobova,O., Heyting,C., Offenberg,H.H., Trump,G.
            and Arnheim,N.
  TITLE     cDNA cloning and characterization of Npap60: a novel rat nuclear
            pore-associated protein with an unusual subcellular localization
            during male germ cell differentiation
  JOURNAL   Genomics 40 (3), 444-453 (1997)
   PUBMED   9073512
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC059239.1.
            
            On Oct 29, 2003 this sequence version replaced NM_016714.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059239.1, BC065102.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 40.25 cM"
     gene            1..4766
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /note="nucleoporin 50"
                     /db_xref="GeneID:18141"
                     /db_xref="MGI:MGI:1351502"
     exon            1..223
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     exon            224..302
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..1634
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /note="nucleoporin Nup50; 50 kDa nucleoporin;
                     nucleoprotein 50; nuclear pore associated protein 60 kDa"
                     /codon_start=1
                     /product="nuclear pore complex protein Nup50"
                     /protein_id="NP_057923.2"
                     /db_xref="CCDS:CCDS27714.1"
                     /db_xref="GeneID:18141"
                     /db_xref="MGI:MGI:1351502"
                     /translation="MAKRVAEKELTDRNWDEEDEVEEMGTFSVASEEVMKNRAVKKAK
                     RRNVGFESDSGGAFKGFKGLVVPSGGGGFSGFGGSGGKPLEGLTNGNSTDNATPFSNV
                     KTAAEPKAAFGSFAVNGPTTLVDKKISSPKCNNSNQPPSSGPASSTACPGNAYHKQLA
                     GLNCSVRDWIVKHVNTNPLCDLTPIFKDYERYLATIEKQLENGGGSSSESQTDRATAG
                     MEPPSLFGSTKLQQESPFSFHGNKAEDTSEKVEFTAEKKSDAAQGATSASFSFGKKIE
                     SSALGSLSSGSLTGFSFSAGSSSLFGKDAAQSKAASSLFSAKASESPAGGGSSECRDG
                     EEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGVGTLHLKPTATQKTQLL
                     VRADTNLGNILLNVLIAPNMPCTRTGKNNVLIVCVPNPPLDEKQPTLPATMLIRVKTS
                     EDADELHKILLEKKDA"
     exon            303..386
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     exon            387..570
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     exon            571..1230
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1312
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1431
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..4749
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1772..1777
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /note="hexamer: AATAAA"
     polyA_site      1795
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /note="major polyA site"
     regulatory      1825..1830
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /note="hexamer: AATACA"
     polyA_site      1851
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
     regulatory      2336..2341
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /note="hexamer: ATTAAA"
     polyA_site      2370
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
     regulatory      4726..4731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
                     /note="hexamer: AATAAA"
     polyA_site      4749
                     /gene="Nup50"
                     /gene_synonym="1700030K07Rik; Npap60"
ORIGIN      
        1 gccccgggcg ctctcttcac cgttctgtag cagcttcggg ctgagcggat gtctcttctt
       61 gtcctcagtg tcggactcag agacacacgg ctcccgagtt ctgctgatca cgaagttccc
      121 ggaggcgctc gacgcaccgg aatctcccag cggccgcgac cgccgcctcg gccctgctcg
      181 ccgcggcgcc gggactccag cgtgatcggc ggcggcagtc aaggttcaca aaaatggcga
      241 agagagttgc ggagaaggag ttgactgaca ggaactggga tgaggaagac gaagttgaag
      301 agatgggaac attctcagtg gccagtgagg aagtcatgaa gaacagagcc gtaaagaagg
      361 caaagcgcag aaacgttgga tttgaatctg atagcggagg agcctttaaa ggtttcaaag
      421 gtttggttgt gccttctgga ggaggagggt tttctggatt tggtggctct ggaggaaagc
      481 ctctggaagg actgacaaat ggaaacagca cagacaatgc cacgcccttc tccaatgtaa
      541 agacagcagc agagcccaag gcagcctttg gttcttttgc tgtgaatggc cctactacct
      601 tggtggataa aaagatttca agtcccaaat gcaataacag caatcagccg ccctcctccg
      661 gccctgcttc cagtaccgcc tgccctggga atgcctatca caagcagctg gctggcttga
      721 actgctccgt ccgcgattgg atagtgaagc acgtgaacac aaacccgctt tgtgacctga
      781 ctcccatttt taaagactat gagagatact tggcgacgat cgagaagcag cttgagaatg
      841 gaggcggcag cagttctgag agccagacag acagggcgac ggctggaatg gagcctcctt
      901 ccctttttgg ttcaacaaaa ctacagcaag agtcaccatt ttcatttcat ggcaacaaag
      961 cggaggacac atctgaaaag gtggagttta cagcagaaaa gaaatcggac gcagcacaag
     1021 gagcaacaag tgcctcgttt agtttcggca agaaaattga gagctcggct ttgggctcgt
     1081 taagctctgg ctccctaact gggttttcat tctctgctgg aagctccagc ttgtttggta
     1141 aagatgctgc ccagagtaaa gcagcctctt cgctgttctc tgctaaagca tccgagagtc
     1201 cggcaggagg cggcagcagc gagtgcagag atggtgaaga agaggagaat gacgagccac
     1261 ccaaggtagt ggtgaccgaa gtaaaggaag aggatgcttt ctactccaaa aaatgtaaac
     1321 tattttacaa gaaagacaac gaatttaaag agaagggtgt ggggaccctg catttaaaac
     1381 ccacagcaac tcagaagacc cagctcttgg tgcgggcaga caccaaccta ggcaacatac
     1441 tgctgaatgt tctgatcgcc cccaacatgc cgtgcacccg gacaggaaag aacaacgtcc
     1501 ttatcgtctg tgtccccaac cccccactcg atgagaagca gcccactctc ccggccacca
     1561 tgctgatccg ggtgaagacg agcgaggatg ccgatgaatt gcacaagatt ttactggaga
     1621 aaaaggatgc ctgagcactg aggctgacca aggcatgttg ccacgttgct gcttcccctc
     1681 cgtccctaac ttagtcacat tctttcctct tctactgtga cattctgaga acttctaggt
     1741 aacttgaact tttgtgagga agattaaggc caataaatcc tttcagtgtg tcgaagctct
     1801 tctcccttcc taagaactaa gcaaaataca ttggagtgaa aagtgtggga agatggtttt
     1861 taatgttgat tcattgagta aactaaccta agtgattctt aatggactat aatcagggta
     1921 ccagttagct ctccaaaggc tccctcaggc agcagtggtg ccgctctctg ccttccccag
     1981 gagacccagt gttcctgaag acaccgcccg tttccttcct gtgggcttgt ctgaccatga
     2041 ctagcactcc tgccagacag cacaccgcca cactgtgacc gaccgcgctt gcccattcct
     2101 tctgagctga ctgggtactg ggaatctgtc gtcagcctgt ctcggctggt gctggccttt
     2161 gacctggagc tgcttgactg aaccaggaac cgcctttcca tgggaaagac catgagtggt
     2221 ggctcgtggg gagctggcgg tggacagact gggtctggag tttatccatg aggtgctctt
     2281 actagctttc ctccctagtt agcatggacg ggggctgtca ggaataatgg attttattaa
     2341 aagcacaaat ttggtagtgt ttttatatca tttctatacc acaaataagc ctttatttta
     2401 aaagaaaaaa gtgaaatcgt gctgaaaagg gttgtgtgct catggccgtt tgtgccctgc
     2461 tgagccctgg tgggcacagt ctgaatggag ttctctcacg agccgtgtgc tcatgagaag
     2521 caagagtatg ggagtggaag acggctcatt tggcccgtag cttcagacag cagcatatta
     2581 tttggcgtct gaagaaaaca tttttatctt tactttttta aggatgaggt attattgagt
     2641 atgtccattt ggaagagagc catggttcta cacgctggtc atgttacctc agagcggtga
     2701 ggttgctaat actagtattc tctgaagacc agacatgggt taaattcgag atttgatgac
     2761 gagctggtca tggttcctgt ctaatcccgg tgctggaaaa gctgaggcag gaagatcatt
     2821 tgagttcaca gcagcttgag ctactgtaag acacctcccc cacccccaat aaaatcaaat
     2881 agaatagaaa gtttgactct ttaaaagtcc acttgcattt ctcataaaac catttgacac
     2941 taggaccaga tcttgtctga aggctcaggg tcagtaccca ggggcgcaca cttgtgtgat
     3001 caggttccga caaaagctgg gtggcacagt tgctttctta aagctgccgt gctaggtggg
     3061 atgaaacctg gttaactttt attttcacat gggtcttagt ctcactatgt aaccctggct
     3121 agccttgaac tcagatccac ctgcctctgc ctcctgagcc cagagatgtt cttgaggtgg
     3181 ggtgacagtg ctctgtgcat tttggcacgt atctgcagat gcatgtttgt gcttcttggg
     3241 ctgtggacct aaggagatgc tgtgcggttt ggtttggttt tgccttgtag catggagaat
     3301 tggttcctag caagcaggtt gctgcttctg ttggccctgg ccctggcttc ttcacattgg
     3361 gcttcccagc tttccaaaga tgaaacaagg ggtgtgcatt tcagaacagt actgtgtagc
     3421 cacgtgcctg tggggctgac acgtgtcaaa ctcacaggca ctttcaggaa cttgtctctt
     3481 catggtggca gtgctctgtg agcatctgcc catgccttga atatttacca aaagaataaa
     3541 agcaaaaaag atcatgactg gactgccttc tagagctccc cggccaattt cagttgtaaa
     3601 ttagtgaggg tggcttgctt ttttttctaa cattccagca atttgctgct aaagactatc
     3661 acttttggaa acaagaatta caggggagat gtgtggggtg tgactgtaac tcggtgtctt
     3721 gtctttatgc tttaggagct agctcattgc atctgacctg agtatgggaa tcactatgaa
     3781 gtctgagtgt cacagggagg cccccggagg gcactctgtt cttcttccac tgtgtgtgta
     3841 gctggttcca acccatggct taaacttcat ttatggttta acgtgttaga aacttgcatc
     3901 ttgtctgttt aatgtgaact gaggtgtgta tctccagttt gaaaatctca cgttgtgtct
     3961 tacatttgcc tgaaggaagt gtgtgttctc tgtggtcagg gtgcatctgg cagatgctaa
     4021 gcctgggcct tcccacacta tagctcacac tatgacacat tcccagccct gccatgtcct
     4081 cctcagagag ccacactgta caggctgtgt ctgtgtcagt gacaggtccg actgttggtg
     4141 ggctgatgct ctgttttacc atttcagttc tgaatcctgt ttttttacat gctgtgtgcc
     4201 aaaaaccaaa acaaaccaac aacaacaaca aaaaaaatac ctcctatggc atagagggga
     4261 attcaccttg ctgtcaggga aagccgtttc cctacctttt gaacattacg aagtctgttc
     4321 ttgccagttt cttcattatg ttactatctc tgaagatgag aaacagcccc ttgaggctgc
     4381 agagtaccca cttagaccac tggagtggga ttttaaggag atggcctgct ctgtcacttg
     4441 taggtgaggt tgggagttct tcagggcgtc tttctagcca catgctctgt acacagccac
     4501 caagtgctct ccagaagcaa tcacaagcct gaggactgtc ccttaacaca gatttgtaaa
     4561 agtgacgtca gacttcgtag ccagcacatg gagtgatccg ccttctactc ggaacgggaa
     4621 cttgttttct ccagctaaaa tacttgaatt gtcaggcagt gtaatctcag tgtgtgttag
     4681 cttttgaatg agcctttaag gaagtgtaac aatccatgaa atgtgaataa atggaaagtt
     4741 gaaaaaccaa aaaaaaaaaa aaaaaa
//
