LOCUS       NM_027091               2732 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus nucleoporin 35 (Nup35), transcript variant 1, mRNA.
ACCESSION   NM_027091 XM_355342
VERSION     NM_027091.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Chen,F., Jiao,X.F., Zhang,J.Y., Wu,D., Ding,Z.M., Wang,Y.S.,
            Miao,Y.L. and Huo,L.J.
  TITLE     Nucleoporin35 is a novel microtubule associated protein functioning
            in oocyte meiotic spindle architecture
  JOURNAL   Exp Cell Res 371 (2), 435-443 (2018)
   PUBMED   30195030
  REMARK    GeneRIF: The results suggest that Nup35 is putatively
            phosphorylated and released to the cytoplasm after resumption of
            meiosis, and it regulates spindle assembly and chromosome
            alignment.
REFERENCE   3  (bases 1 to 2732)
  AUTHORS   Parish,I.A., Stamp,L.A., Lorenzo,A.M., Fowler,S.M., Sontani,Y.,
            Miosge,L.A., Howard,D.R., Goodnow,C.C., Young,H.M. and Furness,J.B.
  TITLE     A Novel Mutation in Nucleoporin 35 Causes Murine Degenerative
            Colonic Smooth Muscle Myopathy
  JOURNAL   Am J Pathol 186 (9), 2254-2261 (2016)
   PUBMED   27427419
  REMARK    GeneRIF: Our study also raises the possibility that Nup35
            polymorphisms could contribute to some cases of Chronic intestinal
            pseudo-obstruction
REFERENCE   4  (bases 1 to 2732)
  AUTHORS   Xiao,J., Vemula,S.R., Xue,Y., Khan,M.M., Kuruvilla,K.P.,
            Marquez-Lona,E.M., Cobb,M.R. and LeDoux,M.S.
  TITLE     Motor phenotypes and molecular networks associated with germline
            deficiency of Ciz1
  JOURNAL   Exp Neurol 283 (Pt A), 110-120 (2016)
   PUBMED   27163549
REFERENCE   5  (bases 1 to 2732)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
REFERENCE   6  (bases 1 to 2732)
  AUTHORS   Handa,N., Kukimoto-Niino,M., Akasaka,R., Kishishita,S.,
            Murayama,K., Terada,T., Inoue,M., Kigawa,T., Kose,S., Imamoto,N.,
            Tanaka,A., Hayashizaki,Y., Shirouzu,M. and Yokoyama,S.
  TITLE     The crystal structure of mouse Nup35 reveals atypical RNP motifs
            and novel homodimerization of the RRM domain
  JOURNAL   J Mol Biol 363 (1), 114-124 (2006)
   PUBMED   16962612
  REMARK    GeneRIF: The crystal structure of the Nup35 RNA-recognition motif
            (RRM) domain has been solved; the Nup35 RRM domain monomer adopts
            the characteristic betaalphabetabetaalphabeta topology revealing a
            new homodimerization mode.
REFERENCE   7  (bases 1 to 2732)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   8  (bases 1 to 2732)
  AUTHORS   Suntharalingam,M. and Wente,S.R.
  TITLE     Peering through the pore: nuclear pore complex structure, assembly,
            and function
  JOURNAL   Dev Cell 4 (6), 775-789 (2003)
   PUBMED   12791264
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY272877.1, AK136109.1 and AK145775.1.
            
            On Jun 13, 2010 this sequence version replaced NM_027091.3.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK145775.1, SRR1660819.135623.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                BY272877.1         1-40
            41-1209             AK136109.1         4-1172
            1210-2732           AK145775.1         1158-2680
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 48.31 cM"
     gene            1..2732
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="nucleoporin 35"
                     /db_xref="GeneID:69482"
                     /db_xref="MGI:MGI:1916732"
     exon            1..121
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1059
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="isoform 1 is encoded by transcript variant 1; 35
                     kDa nucleoporin; mitotic phosphoprotein 44; nuclear pore
                     complex protein Nup53"
                     /codon_start=1
                     /product="nucleoporin NUP35 isoform 1"
                     /protein_id="NP_081367.1"
                     /db_xref="CCDS:CCDS16179.1"
                     /db_xref="GeneID:69482"
                     /db_xref="MGI:MGI:1916732"
                     /translation="MAAFAVDPQAPTLGSEPMMLGSPTSPKTGANAQFLPGFLMGDLP
                     APVTPQPRSISGPSVGVMEMRSPLLAGGSPPQPVVPAHKDKSGAPPVRSIYDDISSPG
                     LGSTPLTSRRQANISLLQSPLVGATTPVPGQSMFSPANIGQPRKTTLSPAQLDPFYTQ
                     GDSLTSEDHLDDTWVTVFGFPQASASYILLQFAQYGNILKHVMSNTGNWMHIRYQSKL
                     QARKALSKDGRIFGESIMIGVKPCIDKNVMENSDRGVLSSPSLAFTTPIRTLGTPTQS
                     GSTPRVSTMRPLATAYKASTSDYQVISDRQTPKKDESLVSRAMEYMFGW"
     exon            122..292
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     exon            293..420
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     exon            421..475
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     exon            476..617
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     exon            618..687
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     exon            688..816
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     exon            817..981
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     exon            982..2732
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1538..1543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="hexamer: AATAAA"
     polyA_site      1567
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="major polyA site"
     regulatory      1799..1804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="hexamer: AATACA"
     polyA_site      1833
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
     regulatory      1862..1867
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="hexamer: AATAAT"
     polyA_site      1891
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
     polyA_site      1945
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
     regulatory      2161..2166
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="hexamer: ATTATA"
     polyA_site      2182
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
     regulatory      2223..2228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="hexamer: TATAAA"
     polyA_site      2244
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
     regulatory      2334..2339
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
                     /note="hexamer: GATAAA"
     polyA_site      2353
                     /gene="Nup35"
                     /gene_synonym="2310006I24Rik; 35kDa; 5330402E05Rik; MP44;
                     NO44"
ORIGIN      
        1 gagcggctct tacggtgata agagtttccg ccatttttga aaactgggaa gctcattgct
       61 ggagtaggag cggccgttgc gatggcagcg tttgcggtgg atccccaggc gcccacgttg
      121 ggatctgaac caatgatgtt gggttcacct acatctccaa agacaggcgc taatgcccag
      181 ttcttgcctg gatttttaat gggggatcta ccagctccag tgactccgca acctcgatca
      241 attagtggcc cttcagtagg agtaatggaa atgagatcac ctttactagc agggggctcc
      301 ccaccacagc cagttgtccc tgctcataaa gataagagtg gagctccacc tgtgagaagt
      361 atctatgatg acatttccag cccaggactt ggatcaacgc ctttgacttc aagaagacag
      421 gcaaacattt cattgttgca gagtcctctt gttggagcta caactcctgt acctgggcaa
      481 agcatgttta gtccagcaaa cattggtcaa ccacgaaaga caacactatc tcctgcccaa
      541 ctggatcctt tttatactca aggagattct ctgacttcag aagatcacct agatgacact
      601 tgggtgactg tgtttgggtt tccacaggca tctgcttctt atatattatt acagtttgca
      661 caatatggga atatcttaaa gcatgtgatg tctaacacag gcaactggat gcatattcgt
      721 taccaatcta aattgcaagc ccggaaagcc ttaagcaaag acgggagaat atttggagaa
      781 tccatcatga ttggtgtcaa gccatgtata gataaaaatg tcatggaaaa cagtgacaga
      841 ggagtcttgt catctccatc tctagccttt acaacaccca tcaggacttt aggcactcca
      901 acacagtccg gaagcactcc tagggtttct accatgaggc ctcttgccac agcatacaag
      961 gcctccacta gtgactacca ggttatttct gacagacaga caccaaagaa ggatgagagc
     1021 cttgtgtcaa gagcgatgga gtacatgttc ggttggtagt acagcaggag ctggactgag
     1081 ccgctatgct acagtagcag agggctgcca tgccatgggt tagttgtata accatcctgg
     1141 cagtgtggga tacccatctc ggatacatct tttacaatcc tttggttaag gaaacagccc
     1201 atctgtagct tgcactttaa aagatggccg tgatagacct gaagagaaga tgcccacatg
     1261 gcatttgtgc ttctgtgatg tagtgcatgc ttagtgccgg gtacccggcg actactgcgg
     1321 tcaattaaat gattgagctt caagtgagtc tcactggctt tcatgagaag gcggatgaat
     1381 ctcagagagt gagagctgct ttaccaagac acagtgatta taattagcaa tttgaattat
     1441 ggtcttaatt tagatagtat ttaatatctt tgtatatgtc ttgtggattg tcatctctta
     1501 tattcttttt aaaaaccatc tacaactaat gtttccaaat aaactttgtc aatttttatg
     1561 tgctaaacat ttgtttggaa ggttgattta ttgttgtagt tttaagagtt cactgaaata
     1621 atagaaaaca tgttagtaac ttatacctag aatccagatt tcattatcat tattacattt
     1681 tgtcatattt gatttccttc ttttgtttga gtacttctca gatatgagat atttttcatc
     1741 caaatatttg gataaataaa aaacatttct atagagtctt aagtttgtta atatgcaaaa
     1801 tacaggtcat ttaaatttgt aatttccccc aaattgacta tttgttgttg tgctttattc
     1861 aaataatgat cttaatgagg ttgccacagt agtaatagtg tcttaatatt taactgttta
     1921 atagttaaat ctgttaaaaa caatagttct ctgtgttcct cactatccaa cactcctggc
     1981 cttggtaaag cagctacgtg agttgtgagt acatagtcta gtgcttggat ttggtcagtt
     2041 gtatgtatat gtgtatgtat gtatgtatgt atgtgtgtat gtatatatgt atatgtatgt
     2101 gtgtatgtat atgtatgtat ttatggttca gcttgtacct cattttttca catcttagcc
     2161 attatatctg gagagaatcc cattctgctt tcattttttt taatagcaat aggtatactt
     2221 tatataaaaa gaagagacac ctaattctcg tcacactttt aaaggtactt atctttatca
     2281 ctggacacag gtacaggcct aatagcttct ttggaacctt ctctgaattc tttgataaaa
     2341 tgtttaaagc ccacgtattt ctgatgctta cctgtatcag ttgtttggag acaataagac
     2401 tttttccagc tgtcactact attgcattta tattcagaat taccctatgt aataggctct
     2461 ttgattacag tagtatacat tcatacagaa ataccaggcc tttcccttta tcatacagaa
     2521 taatgttttc aggccttggt gtcttaaaga acttaaatat aatactatac ctatatttac
     2581 aaagatactc tgtatatata ctatattgaa aacaggtttc attttattat tgtttcttgt
     2641 tatttttttg ttcagattat ctcctttggc tgctaagatt ctcagaaagg gctcttaaaa
     2701 gaaaagaagc ttttaattaa aaaatacagg gc
//
