LOCUS       NM_001355159            2481 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus NUF2, NDC80 kinetochore complex component (Nuf2),
            transcript variant 2, mRNA.
ACCESSION   NM_001355159 XM_006496958
VERSION     NM_001355159.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Yoshida,S., Nishiyama,S., Lister,L., Hashimoto,S., Mishina,T.,
            Courtois,A., Kyogoku,H., Abe,T., Shiraishi,A., Choudhary,M.,
            Nakaoka,Y., Herbert,M. and Kitajima,T.S.
  TITLE     Prc1-rich kinetochores are required for error-free acentrosomal
            spindle bipolarization during meiosis I in mouse oocytes
  JOURNAL   Nat Commun 11 (1), 2652 (2020)
   PUBMED   32461611
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2481)
  AUTHORS   Zhang,T., Zhou,Y., Qi,S.T., Wang,Z.B., Qian,W.P., Ouyang,Y.C.,
            Shen,W., Schatten,H. and Sun,Q.Y.
  TITLE     Nuf2 is required for chromosome segregation during mouse oocyte
            meiotic maturation
  JOURNAL   Cell Cycle 14 (16), 2701-2710 (2015)
   PUBMED   26054848
  REMARK    GeneRIF: Nuf2 is a critical regulator of meiotic cell cycle
            progression in mammalian oocytes.
REFERENCE   4  (bases 1 to 2481)
  AUTHORS   Varma,D. and Salmon,E.D.
  TITLE     The KMN protein network--chief conductors of the kinetochore
            orchestra
  JOURNAL   J Cell Sci 125 (Pt 24), 5927-5936 (2012)
   PUBMED   23418356
  REMARK    Review article
REFERENCE   5  (bases 1 to 2481)
  AUTHORS   Ciferri,C., Pasqualato,S., Screpanti,E., Varetti,G., Santaguida,S.,
            Dos Reis,G., Maiolica,A., Polka,J., De Luca,J.G., De Wulf,P.,
            Salek,M., Rappsilber,J., Moores,C.A., Salmon,E.D. and Musacchio,A.
  TITLE     Implications for kinetochore-microtubule attachment from the
            structure of an engineered Ndc80 complex
  JOURNAL   Cell 133 (3), 427-439 (2008)
   PUBMED   18455984
REFERENCE   6  (bases 1 to 2481)
  AUTHORS   Wigge,P.A. and Kilmartin,J.V.
  TITLE     The Ndc80p complex from Saccharomyces cerevisiae contains conserved
            centromere components and has a function in chromosome segregation
  JOURNAL   J Cell Biol 152 (2), 349-360 (2001)
   PUBMED   11266451
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132467.1 and AK162249.1.
            
            On Sep 9, 2017 this sequence version replaced XM_006496958.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF326732.1, BC020026.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AK132467.1         2-42
            42-1990             AK162249.1         2-1950
            1991-2481           AK132467.1         1916-2406
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 76.84 cM"
     gene            1..2481
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /note="NUF2, NDC80 kinetochore complex component"
                     /db_xref="GeneID:66977"
                     /db_xref="MGI:MGI:1914227"
     exon            1..143
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            144..219
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            220..357
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     CDS             235..1626
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /note="cell division cycle-associated protein 1"
                     /codon_start=1
                     /product="kinetochore protein Nuf2"
                     /protein_id="NP_001342088.1"
                     /db_xref="CCDS:CCDS15463.1"
                     /db_xref="GeneID:66977"
                     /db_xref="MGI:MGI:1914227"
                     /translation="METLSFPRYNVAELVVHIRNKLLTGADGKNLSKSDFLPNPKSDV
                     LYMIYMKALQLVYGVRLEHFYMMPMNIEVTYPHLMEGFLPVRSLFFYMDSFMPICRVN
                     DFEIVDILNPRTNRTSRFLSGIINFIHFRETCLEKCEEFLLQNKSSMVRMQQLSNVHQ
                     EALMKLEKLNTVPAEEREEFKQFMDDIQELQHLLNEEFRQKTTLLQEEYAKMKSDISE
                     KTKHLNEQKLSLVSLKEVEDNLKSKIVDSPEKLKNYKDKMKGTVQKLRSAREKVMEQY
                     DIYRDSVDCLPSCQLEVQLYQKKSQDLADNREKLSSLLKESLNLEDQIESDSSELKKL
                     KTEENSLIRMTTVKKEKLATARFKINKKQEDVKHYKQAMIEDCNKVQEKRDAVCEQVT
                     TVNQEIHKIKSAIQQLRDTKKREILKSQEIFVNLKSALEKYHEGIEKVAEERSAKLEE
                     KTAELKKRMVRMV"
     exon            358..432
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            433..509
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            510..571
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            572..669
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            670..743
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            744..840
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            841..903
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1041
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1182
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1358
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1494
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..2481
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2074..2079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /note="hexamer: AATAAA"
     polyA_site      2104
                     /gene="Nuf2"
                     /gene_synonym="2410003C07Rik; Cdca1; NUF2R"
                     /note="major polyA site"
ORIGIN      
        1 atccgtcgcg aaggaagcgc gctgtgcgga aaggggccgg cctcacaaaa aaaggcggcc
       61 ggtttgaaaa atgacaacgg tcagcggctt tcggttggac gctcggtttt taactgtttt
      121 tccgtgagct gctaggcctc caggaggacc ttgttgagcc tttcctgcgt ctacagaggt
      181 ctgtggtttc ttgaaacaca ggaagcaaca atactcaggg aggagactcc cataatggaa
      241 actttgtcat tccccagata caatgtagct gagcttgtgg ttcatattcg caataaatta
      301 ttaacaggag cagatggcaa aaacctctcg aagagcgatt ttcttccaaa tccgaagtct
      361 gatgtcttgt acatgattta catgaaagcc ttacagttag tgtatggagt ccggctggag
      421 cacttctaca tgatgccgat gaacatagaa gtcacatatc cacatttaat ggagggcttc
      481 ttaccagtca gaagtttgtt cttttacatg gattccttta tgcccatttg ccgggtgaat
      541 gactttgaga ttgtcgacat tcttaatcca agaacaaacc ggacaagtcg ttttttaagt
      601 ggcattatca actttattca cttcagagaa acgtgcctgg agaagtgtga agaatttctt
      661 ttgcaaaata aatcctctat ggtcagaatg cagcagttaa gcaatgtgca ccaggaagct
      721 ttgatgaaac tggaaaaact caataccgtt ccggctgagg agcgagagga gttcaagcag
      781 tttatggatg acattcagga gctgcagcac ctgctgaatg aagagttcag acagaaaacg
      841 acactgctgc aggaggaata tgccaaaatg aaatccgata tttcagagaa aaccaagcat
      901 ttaaatgaac aaaagttgtc attagtttct ttgaaagaag ttgaagacaa tttgaaaagc
      961 aaaattgtgg actctccaga gaagctgaag aactataaag acaagatgaa aggcaccgtc
     1021 cagaagctcc gcagtgccag ggaaaaagtg atggagcagt atgatatcta tagagattct
     1081 gtggattgct tgccttcctg tcagctggag gtgcagttgt atcaaaagaa atcacaggac
     1141 cttgcagata atagggagaa actaagcagt ctcttaaagg agagcttgaa cttggaggac
     1201 cagattgaaa gtgattcatc ggaactaaag aaactgaaga ctgaagaaaa ttctctcata
     1261 agaatgacga ctgtaaagaa ggagaagctc gccaccgcgc gattcaaaat caacaagaag
     1321 caggaggatg tgaaacatta caagcaggcc atgattgaag actgcaataa agttcaagaa
     1381 aagagagatg ctgtctgtga gcaagtaacc actgttaatc aagaaatcca caagattaaa
     1441 tctgcaattc aacaactgag agataccaag aaaagggaga tattgaagtc tcaggagatc
     1501 tttgtaaacc tgaaaagtgc tctggagaag taccatgagg gcatcgagaa ggtagcggag
     1561 gagcgcagcg ctaaactgga ggagaagacg gcagagctga agaagaggat ggtcagaatg
     1621 gtctgaacaa caacagccct tctggaaacg gcctgctgcc tttctgcttc tgtttggcat
     1681 agttatattg aagctaatgg aagtacctgc aatatcagct cactaacacg ggtaccacta
     1741 gttcttgttt ctctttggtg tcattttata tggcgatgtg cttcctgtgt gcttccagac
     1801 ctatccctga gttgaaacta ttccaatggc cattcatatt agttctatta caaggctgga
     1861 agagcttaga tcctttctac ccaggcccaa cgactacagc tgatacagct gacataaact
     1921 ctgatggaga tacaactcag ggccttgcat gctacactga cttgctttcc caggctccaa
     1981 catgccatgt attgtataga cttttactac aattcaaata acgtgtacag cttttatgtc
     2041 cctactctct ctctttttct atgtactggt tggaataaat agatactgaa ctctgtgagt
     2101 tttactactg ttatccagga tgttgtttct tcgacgttgg agtcccacat ccttgagcct
     2161 gactgtgccc tacacatttt gattacatgc tcatttgacg cttgctcatt caaccttccc
     2221 tacatatctg attgctgttg tttaggtaga gaattatgat gatctctgaa gacttattag
     2281 caaaaatttg aactgccacc aaaattgaag atgttccaac tggtaataag gacacatgct
     2341 ccactatgtt catagcagcc ttatttatat tagcctgaag ttggaaagaa cctagatgct
     2401 cctcagcaga ggaatggata cagaaaatgt ggtacagtta caatggagta ttacccagct
     2461 attaaaaaca atgaatttat g
//
