LOCUS       NM_027722               3210 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Mus musculus nudix hydrolase 4 (Nudt4), transcript variant 2, mRNA.
ACCESSION   NM_027722 XM_914448
VERSION     NM_027722.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Song,M.G., Li,Y. and Kiledjian,M.
  TITLE     Multiple mRNA decapping enzymes in mammalian cells
  JOURNAL   Mol Cell 40 (3), 423-432 (2010)
   PUBMED   21070968
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   3  (bases 1 to 3210)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   4  (bases 1 to 3210)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   5  (bases 1 to 3210)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK048062.1, AK017075.1 and CJ208434.1.
            
            On Dec 15, 2017 this sequence version replaced NM_027722.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048062.1, BC027209.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-593               AK048062.1         2-594
            594-889             AK017075.1         512-807
            890-1287            AK048062.1         891-1288
            1288-2928           AK048062.1         1290-2930
            2929-3210           CJ208434.1         132-413
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 49.42 cM"
     gene            1..3210
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /note="nudix hydrolase 4"
                     /db_xref="GeneID:71207"
                     /db_xref="MGI:MGI:1918457"
     exon            1..485
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /inference="alignment:Splign:2.1.0"
     CDS             390..929
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /EC_number="3.6.1.52"
                     /EC_number="3.6.1.61"
                     /note="isoform 2 is encoded by transcript variant 2;
                     diphosphoinositol polyphosphate phosphohydrolase 2; nudix
                     motif 4; nucleoside diphosphate-linked moiety X motif 4;
                     diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 2; nudix
                     (nucleoside diphosphate linked moiety X)-type motif 4"
                     /codon_start=1
                     /product="diphosphoinositol polyphosphate phosphohydrolase
                     2 isoform 2"
                     /protein_id="NP_081998.3"
                     /db_xref="CCDS:CCDS24138.1"
                     /db_xref="GeneID:71207"
                     /db_xref="MGI:MGI:1918457"
                     /translation="MKFKPNQTRTYDREGFKKRAACLCFRSEQEDEVLLVSSSRYPDQ
                     WIVPGGGMEPEEEPGGAAVREVYEEAGVKGKLGRLLGIFENQDRKHRTYVYVLTVTEI
                     LEDWEDSVNIGRKREWFKVEDAIKVLQCHKPVHAEYLEKLKLGCSPTNGNSSVPSLPD
                     NNALFVTAAPPSGVPSSIR"
     exon            486..596
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /inference="alignment:Splign:2.1.0"
     exon            597..641
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /inference="alignment:Splign:2.1.0"
     exon            642..726
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /inference="alignment:Splign:2.1.0"
     exon            727..3210
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1092..1097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /note="hexamer: AACAAA"
     polyA_site      1110
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /note="major polyA site"
     regulatory      3190..3195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /note="hexamer: AATAAA"
     polyA_site      3203
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /note="major polyA site"
     polyA_site      3210
                     /gene="Nudt4"
                     /gene_synonym="4933436C10Rik; DIPP-2; DIPP2; DIPP2alpha;
                     DIPP2beta; HDCMB47P"
                     /note="major polyA site"
ORIGIN      
        1 atcctcggcg tctctgcccg gtctgcaccg tcgcttccaa ctgtctccgc gtggcacgag
       61 ccacgagccg cgctcttccc ccggcggcgg cggcggcggc gggtgcaagc aaggagtggc
      121 tgcgagcggc ggcgggctcc atggagagcg gcgggcgccg gagcagaggc ggcgcttcca
      181 ccgctgcgcc cggactgcag tgacccgcgt tccgccccgc gtcccggcgg ccgcccggcc
      241 tcgccgtccg tgtccgtgcc gccgccgccg cccgtcccac cggcgcccga gcatcgcgac
      301 ttccttcggc ccgactcccc ggcggggtgg cggcggccgg gtccccacgg tggcggccgg
      361 agcagcggct gcaggagccc ggctctagga tgaagttcaa gcccaaccag acgcggacgt
      421 acgaccgcga gggcttcaag aagagggcgg cctgcctgtg cttccgcagc gagcaggagg
      481 acgaggtgct gctggtgagt agcagtcggt acccagacca atggattgtc ccaggaggag
      541 ggatggagcc ggaggaggag cctggcggtg ctgctgtgag ggaagtgtat gaagaggctg
      601 gagtcaaagg aaagctaggc agactgctcg gaatatttga gaatcaagac cggaagcaca
      661 gaacatatgt ttatgttctg actgtcactg aaatattgga agactgggaa gactccgtga
      721 atataggaag gaagagagag tggttcaaag tagaagatgc aatcaaggtt cttcagtgtc
      781 ataagcctgt ccacgcagag tatctggaaa aactaaagct gggctgttct ccgaccaatg
      841 gaaattcctc ggtcccttcc ctcccagata acaatgcctt gtttgtgact gctgcgccgc
      901 cctctggggt gccatccagt ataagataga gggttggggt ccctccccca tcatgtgatg
      961 cacatgtcac aaggagaggc tctttcactt ctaacacacc tgactgctgc tggcaaactc
     1021 tgtatttgct atgcagggat ttcaaataat ttgcatgtat ttcatctgct ttcaagtctt
     1081 ttaaaataaa taacaaaata gcaaaaaata ttttaagatg ccaaagccat gtggaatttt
     1141 ttttaaagcc ttcattgtac cccattcctt tggttttcat ttttataacc tttttgacat
     1201 tttcactttt gtccacttaa cattctatgg tttgttttga cagatcaact ggttattggg
     1261 tatatttttt gcccaatttt tttttttctt acagtatcaa aatagacacc aaatctttgt
     1321 tgggaattaa tgaaaaattc ttggtattta aagaaatcta tccaccccaa aacttaaaga
     1381 gagtcatatt ctccctttgt aaactgcaga tttgggccgt gtttgtatgg gccaggagcc
     1441 tttgacaaag cacttcagct tcctgtttct gagaatttgt tcacaggcag aattatgtca
     1501 cggtccatgt actaggaccg gttcccctct gccacagtga tttgatgtga aagtgtgccg
     1561 tcttttaggg aaaactgtca gtacttacac gtggctctgc atagctcatc ctgaaactgg
     1621 ttatagaaat ctatttagca cgctcagctg ctatttccct tctttgacat agactctatt
     1681 aggggccaca tttccctcaa gggtgagtac tctttaaata tattttcagt ttctgattta
     1741 gagacattgt tcaccactgg actagactaa ccttggatgc cggtcagtga cgactttgtc
     1801 acactaaata agatggctgt tctaggccta agacactgtc ttgagggtca gtaagtgacg
     1861 agtcagatga gttaactgtg gggaaactag aacattcgag ccaagaatta ttgtacctcg
     1921 cctttgtgct gactcaaaat tggccatccc atgaaaaagt accatgactt catggaggaa
     1981 gtgaaggggg agaagaggaa aagatcttta gccattatta accttttgta aatactctaa
     2041 ctggcaagct taagacagtg aagacatttg ttctgtcgct tgttaacctt taaactgtaa
     2101 tagtgctgct gacaagggtc cttgcctgta aggaaaactg gcaccgtttc cataatgaga
     2161 tcttgggcca ccgatgcagc agaactaaaa gcattagatt ccaaagcaga agaactcagc
     2221 cagtagtact cattctaaat tatgtagtgt aaccaagcgt gatcgtccag accttttata
     2281 tttttctttg tacagaggtc atgtactctg ggtgttttaa aagaaacatt ttaaagaaac
     2341 ccacagattg tcattctcta tcttgttttc tcacgattct ctgcaattct tccctgtact
     2401 aaacacaaca gagaaactgg actgttttgt atagaatact gcttccagca aataaaccat
     2461 cacaagtgct tgggggtttg tatgtacacg atactctcag aggtaataat gcatgaacct
     2521 attttatatg taaaatatgt acagtattca tgtcaaccat ctctttaaaa gttagcctat
     2581 aaatattgtt gtataatttc tttggtcaga aacatttgga ctaagtcatg tcacctgggt
     2641 caggatttct ttcagtgcca tttagcaaac tgtctttagt ctatgcaaat agcaaagatt
     2701 tacagaatgc aactgaatgc agcttcactg ttaaatttca cctgagggct cctcattatt
     2761 cttctggaac ccagaacttg aaagatctaa aggaaattga atctagtaga gctcatgtct
     2821 accagcccag ccgcctgcag cggggagact gctgtttgtg cttatctgtg caggcgctga
     2881 ataaaacggg gcagctaaat tctccagttc catgtgcctc ctaatgttta aaagaaaaaa
     2941 gcaaagtaac ttgttaaaaa ttgacttgca aaaaagtaga aactgttggg tggtggtgta
     3001 aagaaatggc attttcccac caaggacaag ccatccagtt gtcagtcaca ccgtatgaag
     3061 cagcaaaggt gaaggagaga aaagggtatg tgtacaagag ttgctttgca tggtctcatc
     3121 tgagatggtt gcattcgaac tgttgtaaga attgtaagaa tcttgacttt tttacgtttg
     3181 gaaacatcaa ataaaaatgg aaacaacctc
//
