LOCUS       NM_026623               1111 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus nudix hydrolase 21 (Nudt21), mRNA.
ACCESSION   NM_026623
VERSION     NM_026623.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Liu,X., Xie,H., Liu,W., Zuo,J., Li,S., Tian,Y., Zhao,J., Bai,M.,
            Li,J., Bao,L., Han,J. and Zhang,Z.C.
  TITLE     Dynamic regulation of alternative polyadenylation by PQBP1 during
            neurogenesis
  JOURNAL   Cell Rep 43 (8), 114525 (2024)
   PUBMED   39037895
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1111)
  AUTHORS   Li,N., Cai,Y., Zou,M., Zhou,J., Zhang,L., Zhou,L., Xiang,W., Cui,Y.
            and Li,H.
  TITLE     CFIm-mediated alternative polyadenylation safeguards the
            development of mammalian pre-implantation embryos
  JOURNAL   Stem Cell Reports 18 (1), 81-96 (2023)
   PUBMED   36563685
REFERENCE   4  (bases 1 to 1111)
  AUTHORS   Alcott,C.E., Yalamanchili,H.K., Ji,P., van der Heijden,M.E.,
            Saltzman,A., Elrod,N., Lin,A., Leng,M., Bhatt,B., Hao,S., Wang,Q.,
            Saliba,A., Tang,J., Malovannaya,A., Wagner,E.J., Liu,Z. and
            Zoghbi,H.Y.
  TITLE     Partial loss of CFIm25 causes learning deficits and aberrant
            neuronal alternative polyadenylation
  JOURNAL   Elife 9, e50895 (2020)
   PUBMED   32319885
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1111)
  AUTHORS   Weng,T., Huang,J., Wagner,E.J., Ko,J., Wu,M., Wareing,N.E.,
            Xiang,Y., Chen,N.Y., Ji,P., Molina,J.G., Volcik,K.A., Han,L.,
            Mayes,M.D., Blackburn,M.R. and Assassi,S.
  TITLE     Downregulation of CFIm25 amplifies dermal fibrosis through
            alternative polyadenylation
  JOURNAL   J Exp Med 217 (2) (2020)
   PUBMED   31757866
  REMARK    GeneRIF: Downregulation of CFIm25 amplifies dermal fibrosis through
            alternative polyadenylation.
REFERENCE   6  (bases 1 to 1111)
  AUTHORS   Weng,T., Ko,J., Masamha,C.P., Xia,Z., Xiang,Y., Chen,N.Y.,
            Molina,J.G., Collum,S., Mertens,T.C., Luo,F., Philip,K., Davies,J.,
            Huang,J., Wilson,C., Thandavarayan,R.A., Bruckner,B.A.,
            Jyothula,S.S., Volcik,K.A., Li,L., Han,L., Li,W., Assassi,S.,
            Karmouty-Quintana,H., Wagner,E.J. and Blackburn,M.R.
  TITLE     Cleavage factor 25 deregulation contributes to pulmonary fibrosis
            through alternative polyadenylation
  JOURNAL   J Clin Invest 129 (5), 1984-1999 (2019)
   PUBMED   30830875
  REMARK    GeneRIF: CFIm25 down-regulation is a novel mechanism to elevate
            pro-fibrotic gene expression in pulmonary fibrosis
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1111)
  AUTHORS   Brumbaugh,J., Di Stefano,B., Wang,X., Borkent,M., Forouzmand,E.,
            Clowers,K.J., Ji,F., Schwarz,B.A., Kalocsay,M., Elledge,S.J.,
            Chen,Y., Sadreyev,R.I., Gygi,S.P., Hu,G., Shi,Y. and
            Hochedlinger,K.
  TITLE     Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation
            to Chromatin Signaling
  JOURNAL   Cell 172 (1-2), 106-120 (2018)
   PUBMED   29249356
  REMARK    GeneRIF: Nudt21 directs differential polyadenylation of over 1,500
            transcripts in cells acquiring pluripotency, although only a
            fraction changed protein levels. These proteins were strongly
            enriched for chromatin regulators.
            Erratum:[Cell. 2018 Jan 25;172(3):629-631. doi:
            10.1016/j.cell.2017.12.035.. PMID: 29373832]
REFERENCE   8  (bases 1 to 1111)
  AUTHORS   Sartini,B.L., Wang,H., Wang,W., Millette,C.F. and Kilpatrick,D.L.
  TITLE     Pre-messenger RNA cleavage factor I (CFIm): potential role in
            alternative polyadenylation during spermatogenesis
  JOURNAL   Biol Reprod 78 (3), 472-482 (2008)
   PUBMED   18032416
  REMARK    GeneRIF: Pre-messenger RNA cleavage factor I (CFIm): potential role
            in alternative polyadenylation during spermatogenesis.
            GeneRIF: Here we report that the CFIm subunits NUDT21/CPSF5 and
            CPSF6 are highly enriched in mouse male germ cells relative to
            somatic cells.
REFERENCE   9  (bases 1 to 1111)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 1111)
  AUTHORS   Kim,H. and Lee,Y.
  TITLE     Interaction of poly(A) polymerase with the 25-kDa subunit of
            cleavage factor I
  JOURNAL   Biochem Biophys Res Commun 289 (2), 513-518 (2001)
   PUBMED   11716503
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138118.4 and AK011688.2.
            
            On Jul 1, 2006 this sequence version replaced NM_026623.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008270.1, AK012308.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-263               AC138118.4         40680-40942
            264-975             AK011688.2         254-965
            976-1111            AC138118.4         58181-58316
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 46.05 cM"
     gene            1..1111
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /note="nudix hydrolase 21"
                     /db_xref="GeneID:68219"
                     /db_xref="MGI:MGI:1915469"
     exon            1..263
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..831
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /note="cleavage and polyadenylation specific factor 5, 25
                     kD subunit; nudix motif 21; nucleoside diphosphate-linked
                     moiety X motif 21; nudix (nucleoside diphosphate linked
                     moiety X)-type motif 21"
                     /codon_start=1
                     /product="cleavage and polyadenylation specificity factor
                     subunit 5"
                     /protein_id="NP_080899.1"
                     /db_xref="CCDS:CCDS40433.1"
                     /db_xref="GeneID:68219"
                     /db_xref="MGI:MGI:1915469"
                     /translation="MSVVPPNRSQTGWPRGVNQFGNKYIQQTKPLTLERTINLYPLTN
                     YTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTT
                     FFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPY
                     IPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQL
                     LSRFNFIYN"
     exon            264..464
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /inference="alignment:Splign:2.1.0"
     exon            465..528
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /inference="alignment:Splign:2.1.0"
     exon            529..618
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /inference="alignment:Splign:2.1.0"
     exon            619..694
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /inference="alignment:Splign:2.1.0"
     exon            695..809
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /inference="alignment:Splign:2.1.0"
     exon            810..1111
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1043..1048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /note="hexamer: ATTAAA"
     polyA_site      1070
                     /gene="Nudt21"
                     /gene_synonym="25kDa; 3110048P04Rik; 5730530J16Rik; Cpsf5"
                     /note="major polyA site"
ORIGIN      
        1 ttccggcgtg tgttccgcgc ctcttgctct cgactgttaa tggcgggtgg tggctgctga
       61 gcgggacgca gataaccgct gcccgccccg cggacgtctc cccgggcccc cgctttctgc
      121 acgtccccgg cgtcggagct gttcagcatg tctgtggtgc cgcccaatcg ctcgcagacg
      181 ggctggcccc ggggggtcaa ccagttcggc aacaagtaca tccagcagac caagcccctc
      241 accctggagc gcaccattaa tctgtacccg cttaccaatt atacttttgg tacaaaggag
      301 cccctctatg agaaggacag ctctgttgca gccagatttc agcgcatgag ggaggaattt
      361 gataagattg ggatgagaag gactgtagaa ggggttctga ttgttcatga acaccgcctg
      421 ccccacgtgc tcctgctgca gctggggaca actttcttca aattacctgg tggggaactt
      481 aacccaggag aagatgaagt tgaaggacta aaacgcttaa tgacagagat acttggtcgt
      541 caagatggag tcctgcaaga ctgggtcatt gatgactgca ttgggaactg gtggagacca
      601 aattttgaac ctcctcagta tccgtatatt cctgcacata taacaaaacc caaggaacat
      661 aagaagttgt ttctggttca gcttcaagag aaagccttgt ttgcagtccc taaaaattac
      721 aagctggtag ctgcaccatt gtttgagctg tatgacaatg caccggggta tggacccatc
      781 atttctagtc ttcctcagct gctgagcagg ttcaatttta tatacaactg aattcctgta
      841 tgcagaagta aaagaagccg tctctatgag cacagcttac acgtgtagaa gagtaactgt
      901 agaacaagtt ttggttttct cttgttccct aaattgccac caccttcctg tttgaagagt
      961 aaaatgaata tgaccgagat agtggtggaa acatgtggca gtgttcatta gcttttggtt
     1021 ctactcatac ttttttttgt acattaaaga aagtgaattt cttatttgta ttttttttta
     1081 agtttctcat taaactctga aaagtcttag a
//
